Medical Sector

The objective of the study is to assess the role of diode laser coupled with topical mitomycin C (MMC) in the management of synechia after endoscopic sinus surgery

Objective: To detect coronary artery disease in asymptomatic patients with systemic lupus erythematosus and scleroderma associated with pulmonary hypertension, and to determine whether it is focal or diffuse ischemia.

Anti-polysaccharide Ab responses in mice are often oligoclonal, and the mechanisms involved in Ag-specific clone production and selection remain poorly understood. We evaluated the relative contribution of D(H) germline content versus N nucleotide addition in a classic oligoclonal, T-independent Ab response (? 1?3 dextran [DEX]) by challenging adult TdT-sufficient (TdT(+/+)) and TdT-deficient (TdT(-/-)) gene-targeted mice, limited to the use of a single D(H) gene segment (D-limited mice), with Enterobacter cloacae. D-limited mice achieved anti-DEX-specific levels of Abs that were broadly comparable to those of wild-type (WT) BALB/c mice.

Two reversed-phase liquid chromatographic (RP-LC) methods have been developed for the determination of sitagliptin phosphate monohydrate (STG). The first method comprised the

Hyaluronic acid application has been proven to be beneficial in a number of medical disciplines. The aim of the current study was to clinically evaluate the effect of local application of hyaluronan gel in conjunction with periodontal surgery. Fourteen patients with chronic periodontitis having four interproximal intrabony defects ?3 mm) with probing depth values >5 mm were included in this split-mouth study. Following initial nonsurgical periodontal therapy and re evaluation, defects were randomly assigned to be treated with modified Widman flap (MWF) surgery in conjunction with either 0.8% hyaluronan gel (test) or placebo gel (control) application. Clinical attachment level (CAL), probing depth (PD), gingival recession (GR), plaque index (PI), and bleeding on probing (BOP) values were taken at baseline and 3 and 6 months. Differences between test and control sites were evaluated using a Wilcoxon signed-rank and a McNemar test. A Friedman and a Cochran test were used to test equal ranks over time. Statistically significant differences were noted for CAL and GR (P<0.05) in favor of the test sites. No significant differences were found regarding PD, BOP, or PI values (P>0.05). Hyaluronan gel application in conjunction with periodontal surgery appears to result in significant improvement of CAL and in a reduction in GR. Hyaluronan gel application appears to improve the clinical outcome of MWF surgery.

Purpose: This study investigated the effect of water storage on nanoleakage depth and the pattern of cervical cavities bonded with different adhesives.

Purpose: There is relatively little information available about the long-term results of total knee arthroplasty (TKA) following high tibial osteotomy. The aim of our study was to share our experiences and long-term results of TKA after a previous closing wedge high tibial osteotomy according to Wagner.

Treatment of the causes of many chronic liver diseases (CLDs) may not be possible. In this case, complications must be anticipated, prevented or at least controlled by the best available therapeutic modalities. There are three main goals for the management of portal hypertension: (i) prevention of the first episode of variceal bleeding largely by non-selective ?-adrenoceptor antagonists, which is not generally recommended in children; (ii) control of bleeding by using a stepwise approach from the least to most invasive strategies; (iii) and prevention of re-bleeding using bypass operations, with particular enthusiasm for the use of meso-Rex bypass in the pediatric population.

The management of children with chronic liver disease (CLD) mandates a multidisciplinary approach. CLDs can be classified into 'potentially' curable, treatable non-curable, and end-stage diseases. Goals pertaining to the management of CLDs can be divided into prevention or minimization of progressive liver damage in curable CLD by treating the primary cause; prevention or control of complications in treatable CLD; and prediction of the outcome in end-stage CLD in order to deliver definitive therapy by surgical procedures, including liver transplantation. Curative, specific therapies aimed at the primary causes of CLDs are, if possible, best considered by a pediatric hepatologist. Medical management of CLDs in children will be reviewed in two parts, with part I (this article) specifically focusing on 'potentially' curable CLDs.

One of the major causes of death is acute renal failure. Hemodialysis was the only way of treatment. The aim of the present study was to evaluate the therapeutic value of mesenchymal stem cells (MSC) and hepatocyte growth factor (HGF) and prediction of their mechanism of action. This study was performed on 50 female albino rats. They were divided equally into 5 groups (10 rats in each group): group (1) control group, group (2) acute renal failure (ARF) group induced by ischemia reperfusion injury, received saline, group (3) ARF group received HGF, group (4) ARF group received MSC, group (5) ARF group received both MSCs & HGF. Blood samples were collected for measurement of urea and creatinine. renal tissue specimens were collected and examined for quantitaive analysis of tumor necrosis factor-? (TNF- ?), interleukin-10(IL-10), vascular endothelial growth factor (VEGF) gene expression by Real Time PCR. Histopathological examination of renal tissue by haematoxylin and eosine and and detection of the MSCs homing in kidney tissue after its labeling with In conclusion PKH26 dye by fluorescent microscope to detect its red fluorescence.

Hop (Humulus lupulus L. Cannabaceae) is an economically important crop. In addition to its role in beer brewing, its pharmaceutical applications have been of increasing importance in recent years. Bitter acids (prenylated polyketides), prenylflavonoids and essential oils, are the primary phytochemical components that account for hop medicinal value. An integrated approach utilizing nuclear magnetic resonance (NMR) and mass spectrometry (MS) techniques was used for the first large-scale metabolite profiling in Humulus lupulus. Resins and extracts prepared from 13 hop cultivars were analysed using NMR, liquid chromatography (LC)-MS and fourier transform ion cyclotron resonance (FTICR)-MS in parallel and analysed by principal component analysis (PCA). A one pot extraction method, compatible with both MS and NMR measurement was developed to help rule out effects due to differences in extraction protocols. Under optimised conditions, we were able to simultaneously quantify and identify 46 metabolites including 18 bitter acids, 12 flavonoids, 3 terpenes, 3 fatty acids and 2 sugars. Cultivars segregation in PCA plots generated from both LC-MS and NMR data were found comparable and mostly influenced by differences in bitter acids composition among cultivars. FTICR-MS showed inconsistent PCA loading plot results which are likely due to preferential ionisation and also point to the presence of novel isoprenylated metabolites in hop.

The increasing interest in the efficacy of phytomedicines and herbal supplements but also the increase in legal requirements for safety and reliable contents of active principles drives the development of analytical methods for the quality control of complex, multi-component mixtures as found in plant extracts of value for the pharmaceutical industry. Here, we describe an ultra-performance liquid chromatography method (UPLC) coupled with quadrupole time of flight mass spectrometry (qTOF-MS) measurements for the large scale analysis of H. perforatum plant material and its commercial preparations. Under optimized conditions, we were able to simultaneously quantify and identify 21 metabolites including 4 hyperforins, 3 catechins, 3 naphthodianthrones, 5 flavonoids, 3 fatty acids and a phenolic acid. Principal components analysis (PCA) was used to ensure good analytical rigorousness and define both similarities and differences among Hypericum samples. A selection of batches from 9 commercially available H. perforatum products available on the German and Egyptian market showed variable quality, particularly in hyperforins and fatty acids. contents. PCA analysis was able to discriminate between various preparations according to its global composition, including differentiation between various batches from the same supplier. To the best of our knowledge, this study provides the first approach utilizing UPLC-MS based metabolic fingerprinting to reveal secondary metabolite compositional differences in Hypericum extract.

Objective: Neonatal sepsis is a major cause of morbidity and mortality worldwide. We analyzed the spectrum of pathogens causing neonatal sepsis in various Arabic countries

Two cyclobenzaprine hydrochloride (CZ) microsized graphite selective sensors wereinvestigated with dibutylsebacate as a plasticizer in a polymeric matrix of carboxylated polyvinyl chloride (PVC-COOH) in the case of sensor 1, based on the interaction between the drug and the dissociated COOH groups in the PVC-COOH. Sensor 2 was based on the interaction between the drug and ammonium reineckate, which acted as anionic electroactive material in the presence of polyvinyl chloride matrix.

Two microsized graphite-design sensors based on ionophore technique, polyvinyl chloride carboxylated (PVC-COOH) and ?-cyclodextrin (?-CD), are used for fabrication of two membrane sensors for the two studied drugs, metronidazole (MZ), sensor 1, and spiramycin (SP), sensor 2. Fast and stable Nernstian responses near 1x10-5- 1x10-3 M for MZ and 1x10-5-1x10-2 M for SP over pH range 5.5-7.5 for the two electrodes reveal the performance characteristics of these electrodes which have been evaluated according to IUPAC recommendations.

Introduction: The purpose of this study was to clinically evaluate miniscrew implant-supported maxillary canine retraction with corticotomy-facilitated orthodontics. Methods: The sample consisted of 13 adult patients (5 men, 8 women; mean age, 19 years) exhibiting Class II Division 1 malocclusion with increased overjet requiring the therapeutic extraction of the maxillary first premolars, with subsequent retraction of the maxillary canines.

Background: Innovative techniques created to restore gastrointestinal perineal continuity after abdominoperineal resection in patients with anorectal cancer include pseudocontinent perineal colostomy, in which the colon is pulled to the perineum and wrapped with a sleeve of stretched colon segment to act as a new sphincter.

Objectives: Detection of telomeric repeat amplification protocol (TRAP) activity, the human telomerase reverse transcriptase (hTERT), tumor suppressor gene (p53) and proliferating marker (MIB-1) in bladder specimens. Design and Methods: Specimens were obtained from 85 patients and 10 controls. TRAP technique and immunohistochemistry (IHC) method were performed.

A series of 1-(2-(4-substitutedarylidene) hydrazinyl-4-(7-chloro- quinolin-4-yl) piperazine-2,5-dione VI was designed by molecular hybridization approach and synthesized for biological evaluation. Virtual screening was carried out through docking the designed compounds into the ATP binding site of epidermal growth factor receptor (EGFR) to predict if these compounds have similar binding mode as the EGFR inhibitors. Subsequently, the compounds were examined for their cytotoxic effect on human breast cell-line (MCF-7) in which the EGFR is highly expressed. Although most of the compounds were quite effective on the breast cancer cell line examined, the compounds II, III, IV a, IVc, VIa, VIc emerged as the most active among the prepared series. Thus 7-chloro-4- (2,5-dioxo 4-substitutedarylidene piperazinoquinoline can serve as the prototype molecule for further development of a new class of EGFR Tyrosine Kinase inhibitors and anti-breast cancer agents.