Mousa, W. M.,
"Evaluation of cercarial antigen for the serodiagnosis of fasciolosis in experimentally and naturally infected sheep",
Veterinary Parasitology, vol. 97, no. 1, pp. 47 - 54, 2001.
AbstractThe value of cercarial antigen for diagnosis of experimental and natural sheep fasciolosis was studied by enzyme linked immunosorbent assay (ELISA) and enzyme linked immunotransfer blot (EITB). In ELISA, the antibody levels of experimentally infected sheep with Fasciola gigantica appeared at 2 weeks post infection (PI), gradually increased till 7 weeks \{PI\} and nearly remained at the same level from 7 to 13 weeks \{PI\} (the end of experiment). Also, the sensitivity and specificity of cercarial antigen for diagnosis of naturally sheep fasciolosis were 100 and 90%, respectively. In EITB, in the sheep experimentally infected with F. gigantica, the band of 32.5 kDa molecular weight polypeptide appeared at 2 weeks \{PI\} and continued till the end of experiment. Also, the cercarial antigen recognized 32.5 kDa molecular weight band with all sera from naturally infected sheep with fasciolosis (n=25). This band did not cross-react when tested with sera from infected sheep with Cysticercus tenuicollus (n=20). This study suggests that, the 32.5 kDa molecular weight polypeptide could be used as sensitive and specific epitope for the serodiagnosis of sheep fasciolosis.
Selim, S. A., M. O. El-Shazly, E. A. Nasr, A. L. El-Naggar, A. W. M. Abdul-Razik, and F.A.,
"Evaluation of enzyme linked immunosorbent assay (ELISA) and pathological findings in the diagnosis of tuberculosis in camel (Camelus dromedarius) in Egypt.",
Bulletin of Animal Health and Production in Africa, vol. 49, no. 2, pp. 84–94, 2001.
Abstractn/a
c Khaled, H. M. a, I. a Abdel-salam, M. a Abdel-gawad, A. A. Metwally, S. a El-demerdash, M. a El-didi, A. a Morsi, and L. b Ishak,
"Evaluation of the BTA tests for the detection of bilharzial related bladder cancer: The Cairo experience",
European Urology, vol. 39, no. 1, pp. 91-94, 2001.
AbstractObjective: To evaluate the clinical performance of the BTA stat test and the BTA TRAK assay in the diagnosis of bilharzia-related bladder cancer and to calculate a new 'Egyptian' cut-off value for the BTA TRAK (quantitative) assay. Methods: Urine samples of 149 individuals were tested for the presence of the human complement factor H-related protein, the antigen detected by the BTA stat and BTA TRAK tests. The group consisted of 53 healthy volunteers, 20 patients with active bilharziasis, 11 patients with other urologic disorders including prostate cancer, and 65 patients with histologically proven bladder cancer. All samples were obtained prior to surgery or therapy. Results: The BTA stat test was positive in 64 of 65 samples from patients with bladder cancer, for an overall sensitivity of 99%. With a BTA TRAK assay cut-off of 60 U/ml (set at 97% specificity in the healthy population), the sensitivity of the TRAK assay was 94%. There was no statistically significant difference between the sensitivities of the two BTA tests in patients diagnosed with squamous cell carcinoma and those with transitional cell carcinoma. The overall specificity of the BTA stat test was 67% ranging from 15% in patients with bilharziasis to 94% in healthy volunteers. The overall specificity of the TRAK assay was 66%, again with negative results in 15% of the patients with bilharziasis. Conclusions: The BTA stat test and TRAK tests are extremely sensitive in the detection of bladder cancer in the Egyptian population. Positive results (85%) are also observed in patients with active bilharziasis, which often leads to bladder cancer. Longitudinal follow-up of these positive cases is needed to determine whether these positive results are false or predictive of bladder cancer. Copyright © 2001 S. Karger AG, Basel.
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