ISSN: ‎2008-4226, Abbreviation: Anal. Bioanal. Electrochem.


1 Analytical chemistry department, Faculty of pharmacy, Cairo University, Cairo, Egypt

2 Pharmaceutical chemistry department, Faculty of pharmaceutical science and pharmaceutical industries, Future University in Egypt, Cairo, Egypt


Direct drug determination without any pervious treatment steps is the most environmentally friendly method of analysis. Traditional analysis methods usually involve a pre-treatment step before analysis and this consumes time and organic solvents. This work describes direct potentiometric method by using ion selective electrode to determine Montelukast Sodium in its pure drug substance and in tablet formulation and for inline monitoring of its release from its tablet form without any sample pretreatment. A Sensor was fabricated using PVC based membrane containing tetradodecyl ammonium bromide (TDB) being as an anionic exchanger and 2-nitrophenyl-octyl-ether (2-NPOE) being a plasticizer. The validation of the proposed method was done according to International union of pure and applied chemistry recommendations, in which the proposed sensor show a linear dynamic range from 1.0×10-6 to 1.0×10-2 mol/L. The proposed sensor was applied to determine Montelukast sodium in bulk powder, tablets dosage form with no extraction. The sensor was also used as bench-top real-time analyser for in process tracking of Montelukast sodium concentration during monitoring of its dissolution behaviour, under U.S. Food and Drug Administration dissolution regulations, with clear discrimination from its common excipients. Results obtained by the proposed potentiometric method were compared with those obtained by the official HPLC method.


[1] B. E.Conway, Ottawa Press 3 (1995) 41.
[2] la Guardia, S. Garrigues, Handbook of green analytical chemistry, Wiley Online Libary (2012).
[3] V. V. Coşofreţ, TrAC, Trends Anal. Chem. 10 (1991) 298.
[4] R.I. Stefan, G.E. Baiulescu, and H.Y. Aboul-enien, Crit. Rev. Anal. Chem. 27 (1997) 307.
[5] V. K. Gupta, A. Nayak, S. Agarwal, and B. Singhal, Comb. Chem. High Throughput Screen. 14 (2011) 284.
[6] I. Narin, S. Sarioglan, and B. Anilanmert, and H. Sari, J. Solution Chem. 39 (2010) 1582.
[7] V. Choudhari, A. Kale, S. Abnawe, V. Gawli, and N. Patil, Int. J. Pharm. Tech. Res. 2 (2010) 4.
[8] Y. Shakalisava, and F. Regan, J. Sep. Sci. 31 (2008) 1137.
[9] J. Roman, M. D. Malesuik, A. Jablonski, and M. Steppe, Die Pharmazie-An Int. J. Pharm. Sci. 65 (2010) 645.
[10] S. Flor, M. Huala Juan, V. Tripodi, and S. Lucangioli, Electrophoresis 37 (2016) 2420.
[11] S. A. Patel, D. J. Patel, and N. J. Patel, Int. Res. J. Pharm. 2 (2011) 154.
[12] M. S. Arayne, N. Sultana, and F. Hussain, J. Anal. Chem. 64 (2009) 690.
[13] K. Pallavi, and S. Babu, Int. J. Adv. Pharm. Biol. Chem. 1 (2012) 104.
[14] Q. N. Rashid, A. H. M. Nasief, Tikret, J. Pharm. Sci. 14 (2019) 18.
[15] S. S. Patil, S. Atul, S. Bavaskar, S. N. Mandrupkar, P. N Dhabale, and B. S. Kuchekar, J. Pharm. Res. 2 (2009) 714.
[16] P. V. Adsule, K. Sisodiya, A. G. Swami, V. P. Choudhari, and B. S. Kuchekar Adsule, Int. J. Pharm. Sci. Rev. Res. 12 (2012) 106.
[17] S. Bhagade, Int. J. Pharm. Sci. Res. 4 (2013) 4432.
[18] J. V. Kumar, D. Ramachandran, K. Sushma, and S. Vijay, Orient J. Chem. 26 (2010) 293.
[19] L. K. Garg, B. R. Kumar, S. S. Sait, and T. Krishnamurthy, Int. J. Pharm. Sci. Rev. Res. 7 (2011) 69.
[20] I. A. lsarra, N. Y. Khalil, M. Sultan, R. Al-Ashban, and F. Belal, Die Pharmazie-An Int. J. Pharm. Sci. 60 (2005) 823.
[21] I. A. Alsarra, I. A., Saudi Pharm J. 12 (2004) 136.
[22] R. M. Singh, P. K. Saini, S. C. Mathur, G. N. Singh, and B. Lal, Ind. J. Pharm. Sci. 72 (2010) 235.
[23] A. K. Shakya, T. A .Arafat, N. M. Hakooz, A. N. Abuawwad, H. Al-Hroub, and M. Melhim, Acta Chromatographica 26 (2014) 457.
[24] M. Thummisetty, J. R. Sama, V. S. N. Rao, and P. Reddanna, J. Pharm. Sci. Res. 3 (2011) 1373.
[25] S. Alireza, Z. Afshin, M. F. Seyed, K. Arash, and M. Babak, J. Bioequiv. Availab. 2 (2010) 135.
[26] D. G. Phadtare, A. R. Pawar, R. R. Kulkarni, G. K. Patil, Asian J. Res. Chem. 9 (2016) 339.
[27] A. B. Eldin, A. A. Shalaby, and M. El-Tohamy, Acta Pharm. Sci. 53 (2011) 45.
[28] A. S. Rathore, L. Sathiyanarayanan, and K. R. Mahadik, Pharm. Anal. Acta (2010) 2153.
[29] R. Sane, A. Menezes, M. Mote, A. Moghe, and G. Gundi, JPC-Journal of Planar Chromatography-Modern TLC 17 (2004) 75.
[30] J. Roman, A. R. Breier, and M. Steppe, J. Chrom. Sci. 49 (2011) 540.
[31] I. Alsarra, M. Al-Omar, E. A. Gadkariem, and F. Belal, Il Farmaco 60 (2005) 563.
[32] A. F. Alghamdi, Portugaliae Electrochim. Acta 32 (2014) 51.
[33] G. Yıldız, Z. Aydoğmuş, and J. M. Kauffmann, Electroanalysis 25 (2013) 1796.
[34] B. Çölkesen, F. Öztürk, and P. E. Erden, J. Braz. Chem. Soc. 27 (2016) 849.
[35] Pharmacopeia, U.S. Official Monographs, Montelukast Sodium. 38th edition (2015).
[36] IUPAC, Analytical chemistry division, commission on analytical nomenclature. Pure Appl.Chem.72 (2000) 1851.
[37] E. Lindner, and Y. Umezawa, Pure Appl. Chem. 80 (2008) 85.
[38] J. Sekula, J. Everaert, H. Bohets, B. Vissers, M. Pietraszkiewicz, O. Pietraszkiewicz, F. D. Prez, K. Vanhoutte, P. Prus, and L. J. Nagels, Anal. Chem. 78 (2006) 3772.
[39] H. Ibrahim, Y. M. Issa, H. M. and Abu-Shawish, J. Pharm. Biomed. Anal. 44 (2007) 8.
[40] T. Masadome, J. Yang, and T. Imato, Microchim. Acta 144 (2004) 217.
[41] I. Švancara, K. Vytras, J. Barek, and J. Zima, Crit. Rev. Anal. Chem. 31 (2001) 311.
[42] S. Ogawara, J. L.Carey III, X. U. Zou, and P. Bühlmann, ACS Sens. 1 (2015) 95.
[43] FDA, U.S., Guidance for industry: Dissolution testing of immediate-release soild oral dosage forms. Food and Drug Administration, Centre for Drug Evaluation and Research (CDER) (1997).
[44] International Conference on Harmonization (ICH), Q2B: Validation of Analytical Procedures: Methodology, 62, US FDA Federal Register, 1997.
[45] T. O'hara, A. Dunne, J. Butler, J. Devane, and IVIVR, Pharm. Sci. Technol. Today 1 (1998) 214.
[46] N. Yuksel, A. E. Kanık, and T. Baykara, Int. J. Pharm. 209 (2000) 57.
[47] A. Gałuszka, Z. M. Migaszewski, P. Konieczka, and J.Namieśnik, TrAC, Trends Anal. Chem. 37 (2012) 61.