@article {153548,
	title = {Anticancer potentiality of lignan rich fraction of six Flaxseed cultivars.},
	journal = {Scientific reports},
	volume = {8},
	year = {2018},
	month = {2018 Jan 11},
	pages = {544},
	abstract = {<p>The objective of our study is to highlight the therapeutic effect and mechanism of action by which purified Flaxseed hydrolysate (PFH) which is a lignan rich fraction exerts its anticancer activity on a human breast cancer cell line (T47D) and in mice bearing tumor. HPLC analysis of PFH of six flaxseed cultivars had shown that PFH of the cultivar Giza 9 (PFH-G9) contains the highest concentration of SDG (81.64 mg/g). The in vitro cytotoxic potentiality of PFH{\textquoteright}s of six flaxseed cultivars was screened against a panel of~human cancer cell lines. PFH -G9 showed the most significant cytotoxic activity against ER-receptor positive breast cell lines MCF7 and T47D with IC 13.8 and 15.8 {\textmu}g/ml, respectively. Moreover, PFH-G9 reduced the expression of the metastasis marker, 1-α, metalloproteinases and vascular endothelial growth factor (VEGF), one of the most potent stimulators of angiogenesis, while it increased the caspase-3 dependent apoptosis. Our study also showed that dietary intake of 10\% of Giza 9 Flaxseeds (FS), fixed oil (FSO) or Flax meal (FSM) twice daily for 3 weeks in mice-bearing solid Ehrlich ascites carcinoma (EAC) resulted in reducing the tumor volume, the expression of estrogen, insulin growth factor, progesterone, VEGF and MMP-2, but enhanced expression of caspase-3.</p>
},
	issn = {2045-2322},
	doi = {10.1038/s41598-017-18944-0},
	author = {Shahira M. Ezzat and Samia A. Shouman and Abeer ElKhoely and Yasmin M Attia and Elsesy, Mohamed S and El Senousy, Amira S and Mouchira A Choucry and Sabah H. Elgayed and El Sayed, Abeer A and Essam Abdelsattar and El Tanbouly, Nebal}
}
