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Abdel Aziz, M. T., A. M. Rezq, H. M. Atta, H. Fouad, AM Zaahkouk, H. H. Ahmed, D. Sabry, and H. M. Yehia, "Molecular signalling of a novel curcumin derivative versus Tadalafil in erectile dysfunction", Andrologia, vol. 47, issue 6, pp. 616-625, 2015.
Hamed, E. A., M. M. El-Saied, K. Saad, H. A. - Z. Yousef, A. O. Mohamed, and D. Sabry, "Molecular mechanisms underlying fibrosis and elastin destruction in childhood interstitial lung diseases.", Pathophysiology : the official journal of the International Society for Pathophysiology, 2016 Sep 21. Abstract

OBJECTIVE: This study aimed to evaluate fibrosis and elastin destruction in childhood interstitial lung disease (chILD) patients.

METHODS: Sixty patients and twenty healthy children were recruited. On admission, evaluation of chILD severity was made using Fan chILD score. Participants provided urine and blood samples. Plasma levels of transforming growth factor (TGF)-β1, connective tissue growth factor (CCN2), soluble factor related apoptosis (sFas) and long non-coding RNAs and urinary levels of desmosine/urinary creatinine (UDes/UCr) were measured.

RESULTS: In patients, clinical findings were crackles (100.00%), tachypnea (65.00%), cardiomegaly (45.00%), digital clubbing (43.30%), cough (33.00%), cyanosis (26.70%), hepatomegaly (28.30%) and wheezes (23.30%). Categorizing of the patients with Fan chILD clinical score revealed that most patients 33.30% scored (3, symptomatic with abnormal saturation/cyanosis during exercise) then 28.30% scored (5, symptomatic with clinical and echocardiographic features of pulmonary hypertension), 18.30% scored (2, symptomatic with normal room air saturations), 15.00% scored (1, asymptomatic) and 5.00% scored (4, symptomatic with abnormal room air saturation/cyanosis at rest). TGF-β1, CCN2, sFas, lncrRNA-2700086A05Rik relative gene expression and UDes/UCr levels were higher in patients than controls (P=0.002, P=0.001, P=0.001, P=0.001, P=0.001, respectively). In patients, significant positive correlations were found between TGF-β1 and CCN2, sFas, UDes/UCr; between CCN2 and both sFas and UDes/UCr; between UDes/UCr and sFas. Morbidity and mortality rates were 46.70% and 10.00%, respectively.

CONCLUSION: Markers of fibrosis (TGF-β1, sFas, CCN2) and elastin destruction (UDes/UCr) were increased in chILD especially in patients with long disease duration. So blockage of their pathways signals may offer novel therapeutic targets.

Salama, M. K., D. Sabry, MA Al-Ghussein, R. Ahmed, S. A. Allah, F. M. Taha, W. Fathy, M. S. Wadie, M. Nabih, A. Abul-Fotouh, et al., "Molecular detection of monocyte chemotactic protein-1 polymorphism in spontaneous bacterial peritonitis patients", World journal of gastroenterology: WJG, vol. 20, issue 33, pp. 11793-11799, 2014.
Abdelsalam, A., L. Rashed, T. Salman, L. Hammad, and D. Sabry, "Molecular assessment of Vitamin D receptor polymorphism as a valid predictor to the response of Interferon/Ribavirin based therapy in Egyptian patients with Chronic Hepatitis C.", Journal of digestive diseases, 2016 Apr 29. Abstract

BACKGROUND/AIM: Egypt has the highest prevalence rate of Hepatitis C virus (HCV) infection around the globe, where, chronic hepatits C (CHC) is considered a major health problem. The standard treatment of CHC is combination therapy of pegylated interferon and ribavirin. Successful treatment and sustained virological response (SVR) are only achieved in 30% of patients. Major adverse effects and high cost of the treatment makes predicting the treatment output is an important approach. The aim of this study to find an association between Vitamin D concentration and vitamin D receptor (VDR) polymorphisms with achieving SVR.

METHODS: This is a case control study in which; 250 patients recruited and were divided into 3 groups (100 CHC patients who achieved SVR, 100 CHC patients who did not achieve SVR, and 50 apparently healthy individuals as control). Blood samples were collected to measure vitamin D concentration and 4 VDR polymorphisms (FokI, ApaI, TaqI, and BsmI) were detected using RFLP-PCR.

RESULTS: Non responders were found to have significantly low vitamin D concentration compared to responders and control groups. Concerning VDR polymorphisms, both FokI and TaqI polymorphisms were associated to successful treatment.

CONCLUSION: Vitamin D concentration, FokI, and TaqI may be considered as one of the predictors for the response of CHC patients to combination of pegylated interferon and ribvirin therapy. This article is protected by copyright. All rights reserved.

Ebrahim, N., E. Ehsan, E. A. Ghany, D. Sabry, and A. Shamaa, "Mesenchymal stem cells transplantation attenuates experimentally induced brain injury after neonatal hypoxia by different two routes of administrations ", Biocell, vol. 43, issue 1, pp. 21-28, 2019.
Ebrahim, N., I. A. Ahmed, N. I. Hussien, A. A. Dessouky, A. S. Farid, A. M. Elshazly, O. Mostafa, W. B. E. Gazzar, S. M. Sorour, Y. Seleem, et al., "Mesenchymal Stem Cell-Derived Exosomes Ameliorated Diabetic Nephropathy by Autophagy Induction through the mTOR Signaling Pathway.", Cells, vol. 7, issue 12, 2018 Nov 22. Abstract

BACKGROUND: Diabetic nephropathy (DN) is a serious complication of diabetes mellitus and a common cause of end-stage renal disease. Autophagy has a defensive role against kidney damage caused by hyperglycemia. Mesenchymal stem cell (MSC)-derived exosomes are currently considered as a new promising therapy for chronic renal injury. However, the renal-protective mechanism of exosomes on DN is not completely understood. We examined the potential role of MSC-derived exosomes for enhancement of autophagy activity and their effect on DN. In our study, we used five groups of rats: control; DN; DN treated with exosomes; DN treated with 3-methyladenine (3-MA) and chloroquine (inhibitors of autophagy); and DN treated with 3-methyladenine (3-MA), chloroquine, and exosome groups. We assessed renal function, morphology, and fibrosis. Moreover, ratios of the autophagy markers mechanistic target of rapamycin (mTOR), Beclin-1, light chain-3 (LC3-II), and LC3-II/LC3-I were detected. Additionally, electron microscopy was used for detection of autophagosomes.

RESULTS: Exosomes markedly improved renal function and showed histological restoration of renal tissues, with significant increase of LC3 and Beclin-1, and significant decrease of mTOR and fibrotic marker expression in renal tissue. All previous effects were partially abolished by the autophagy inhibitors chloroquine and 3-MA.

CONCLUSION: We conclude that autophagy induction by exosomes could attenuate DN in a rat model of streptozotocin-induced diabetes mellitus.

Lobna A Aly, H. E. - Menoufy, Rehab Tarek Elsharkawy, Mona Z Zaghloul, and D. Sabry, "Maternal chronic oral infection with periodontitis and pericoronitis as a possible risk factor for preeclampsia in Egyptian pregnant women (microbiological and serological study)", Future Dental Journal, vol. 1, issue 1, pp. 23-32, 2015.