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Fouad, H., D. Sabry, H. Morsi, H. Shehab, and N. F. Abuzaid, "XRCC1 Gene Polymorphisms and miR-21 Expression in Patients with Colorectal Carcinoma.", The Eurasian journal of medicine, vol. 49, issue 2, pp. 132-136, 2017 Jun. Abstractfinal_reprint_dr._naglaa_md_article_132-136.pdf

OBJECTIVE: The objectives of this study were to evaluate the impact of two X-ray repair cross complementing 1 (XRCC1) gene polymorphisms (Arg194Trp and Arg399Gln) on the risk of development of colorectal cancer (CRC) and to assess the expression levels of microRNA-21 (miR-21) in CRC patients.

MATERIALS AND METHODS: A case-control cross sectional study was conducted on 50 CRC patients and 50 cancer-free subjects. DNA and miR-21 were extracted from whole blood samples. The expression levels of the XRCC1 polymorphisms and miR-21 were assessed by real-time PCR in all subjects of the study.

RESULTS: Genotype analysis revealed a significant association between CRC risk and both the Arg194Trp genotype (OR=11.407, 95% CI=4.039-32.221, p<0.001) and the Arg399Gln genotype (OR=3.778, 95% CI= 1.6-8.919, p=0.002). The expression levels of circulating miR-21 were able to detect CRC cases significantly (p=0.022) with a sensitivity of 82% and a specificity of 56% (Area under the curve (AUC)=0.633) but were unable to distinguish between early and late cases (AJCC classification) (p=0.194).

CONCLUSION: The XRCC1 Arg194Trp and Arg399Gln polymorphisms both confer high susceptibility for the development of CRC. Circulating miR-21 expression levels are a potentially diagnostic non-invasive genetic marker of CRC.

Fouad, H., D. Sabry, K. Elsetohy, N. Fathy, and D. I. N. A. OMAR, "Potential therapeutic effect of amniotic stem cell transplantation on ovarian function and folliculogenesis in rats with induced ovarian failure", Journal of Evidence-Based Women’s Health Journal Society, vol. 5, issue 3, pp. 99-110, 2015.
Fouad, H., D. Sabry, K. Elsetohy, and N. Fathy, "Therapeutic efficacy of amniotic membrane stem cells and adipose tissue stem cells in rats with chemically induced ovarian failure", Journal of Advanced Research, 2016.
Fouad, H., D. Sabry, K. Elsetohy, and N. Fathy, "Therapeutic efficacy of amniotic membrane stem cells and adipose tissue stem cells in rats with chemically induced ovarian failure.", Journal of advanced research, vol. 7, issue 2, pp. 233-41, 2016 Mar. Abstract

The present study was conducted to compare between the therapeutic efficacies of human amniotic membrane-derived stem cells (hAM-MSCs) vs. adipose tissue derived stem cells (AD-MSCs) in cyclophosphamide (CTX)-induced ovarian failure in rats. Forty-eight adult female rats were included in the study; 10 rats were used as control group. Thirty-eight rats were injected with CTX to induce ovarian failure and divided into four groups: ovarian failure (IOF) (IOF group), IOF + phosphate buffer saline (PBS group), IOF + hAM-MSCs group and IOF + AD-MSCs group. Serum levels of FSH and estradiol (E2) were assessed. Histopathological examination of the ovarian tissues was performed and quantitative gene expressions of Oct-4, Stra8 and integrin beta-1 genes were conducted by quantitative real time PCR. Results showed that IOF and IOF + PBS rat groups exhibited decreased ovarian follicles, increased interstitial fibrosis with significant decrease of serum E2, significant increase serum FSH level and significant down-regulation of Stra8 and integrin beta-1. In hAM-MSCs and AD-MSCs rat groups, there were increased follicles and corpora with evident the presence of oocytes, significant increase in serum E2, significant decrease in serum FSH levels (in hAM-MSCs treated group only) and significant up-regulation of the three studied genes with higher levels in hAM-MSCs treated rats group when compared to AD-MSCs treated rats group. In Conclusion, administration of either hAM-derived MSCs or AD-MSCs exerts a significant therapeutic efficacy in chemotherapy induced ovarian insult in rats. hAM-MSCs exert higher therapeutic efficacy as compared to AD-MSCs.

Fouad, R., M. Abdo, H. G. E. deen, D. Sabry, Mira Atef, R. Ahmed, and N. Zayed, "Influence of delta virus infection on the virologic status in Egyptian patients with chronic Hepatitis B virus genotype D", J Med Virol., vol. 88, issue 5, pp. 837-42, 2016.