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A, F., S. D, A. R, K. M, Allah SA, Marzouk S, A. M, Abd El Aziz R, El Badri A, K. H, et al., "Comparative diagnostic study of biomarkers using FibroMax™ and pathology for prediction of liver steatosis in patients with chronic hepatitis C virus infection: an Egyptian study.", Int J Gen Med, vol. 12, issue 6, pp. 127-34, 2013.
Abdel, A. M. T., F. M. El-Asmar, T. Mostafa, H. Atta, H. H. Fouad, N. K. Roshdy, L. A. Rashed, E. A. Obaia, D. A. Sabry, A. A. T. Abdel, et al., "Effect of hemin and carbon monoxide releasing molecule (CORM-3) on cGMP in rat penile tissue.", The journal of sexual medicine, vol. 5, no. 2, pp. 336–343, 2008. Abstract
Abdel Aziz, M. T., T. Mostafa, H. Atta, O. Kamal, M. Kamel, H. Hosni, L. Rashed, D. Sabry, and F. Waheed, "Heme oxygenase enzyme activity in seminal plasma of oligoasthenoteratozoospermic males with varicocele", Andrologia, vol. 42, no. 4: Wiley Online Library, pp. 236–241, 2010. Abstract
Abdel Aziz, M. T., A. M. Rezq, H. M. Atta, H. Fouad, AM Zaahkouk, H. H. Ahmed, D. Sabry, and H. M. Yehia, "Molecular signalling of a novel curcumin derivative versus Tadalafil in erectile dysfunction", Andrologia, vol. 47, issue 6, pp. 616-625, 2015.
Abdel Aziz, M. T., T. Mostafa, H. Atta, L. Rashed, S. A. Marzouk, E. M. Obaia, D. Sabry, A. A. Hassouna, A. M. El-Shehaby, and A. T. Abdel Aziz, "Oral phosphodiesterase-5 inhibitors: effect of heme oxygenase inhibition on cGMP signalling in rat cavernous tissue", Andrologia, vol. 39, no. 2: Wiley Online Library, pp. 66–70, 2007. Abstract
Abdel Aziz, M. T., S. El-Haggar, T. Mostafa, H. Atta, H. Fouad, S. Mahfouz, L. Rashed, D. Sabry, A. Senbel, and G. A. Ali, "Effect of mesenchymal stem cell penile transplantation on erectile signaling of aged rats", Andrologia, vol. 42, no. 3: Wiley Online Library, pp. 187–192, 2010. Abstract
Abdel Aziz, M. T., H. M. Atta, S. Mahfouz, H. H. Fouad, N. K. Roshdy, H. H. Ahmed, L. A. Rashed, D. Sabry, A. A. Hassouna, and N. M. Hasan, "Therapeutic potential of bone marrow-derived mesenchymal stem cells on experimental liver fibrosis", Clinical biochemistry, vol. 40, no. 12: Elsevier, pp. 893–899, 2007. Abstract
Abdel Aziz, M. T., T. Mostafa, H. Atta, M. A. Wassef, H. H. Fouad, L. A. Rashed, and D. Sabry, "Putative role of carbon monoxide signaling pathway in penile erectile function", The journal of sexual medicine, vol. 6, no. 1: Wiley Online Library, pp. 49–60, 2009. Abstract
Abdelfattah, D. S., R. Sayed, G. Mostafa-Hedeab, and H. H. M. Ali, "In Vitro Characterization and Evaluation of Silver Nanoparticles Cytotoxicity on Human “Liver and Breast” Cancer Cells Versus Normal Melanocytes ", The Egyptian journal of histology, vol. 42, issue 3, pp. 755-766, 2019.
Abdelgawad, L. M., M. M. Abd El-Hamed, D. Sabry, and M. Abdelgwad, "Efficacy of Photobiomodulation and Metformin on Diabetic Cell Line of Human Periodontal Ligament Stem Cells through Pathway.", Reports of biochemistry & molecular biology, vol. 10, issue 1, pp. 30-40, 2021. Abstract

Background: Diabetes mellitus (DM) is a metabolic disorder resulting from hyperglycemia. Hyperglycemia contributes to oxidative stress, and the release of advanced glycation end products (AGEs) further promotes disease pathogenesis. Uncontrolled diabetes reflects great oral complications and affects human oral health. So, the present study aimed to assess the effects of photobiomodulation therapy (PBMT) and Metformin on proliferation and viability of human periodontal ligament stem cells (HPDLSCs) cultured in high glucose medium.

Methods: HPDLSCs were collected, isolated, and characterized and then divided into eight groups. Addition of extra glucose to diabetic groups 24 hours before cell irradiations. Metformin was added to half of the diabetic groups. Cells were irradiated with 808 nm diode laser 24, 48 hours. Cell viability was analyzed with MTT assay 24 hours post-irradiation to detect cell viability in each group. Real-time (PCR) was used to evaluate gene expression of and and the effect of PBMT on Pathway. ELISA reader was used to evaluating cell viability through (ROS, TNF-α, IL-10) protein levels after cell irradiation.

Results: Photobiomodulation at 1, 2, and 3 J/cm2 combined with metformin significantly promoted diabetic cell lines of HPDLSCs viability (in MTT assay and ELISA reader of ROS, TNF-α, IL-10 results) and gene expression of , and levels (p< 0.05).

Conclusion: photobiomodulation with 3 J/cm combined with metformin enhanced proliferation and viability of diabetic cell lines of HPDLSCs and thus could improve differentiation and function of diabetic cell lines of HPDLSCs with minimum side effects.

Abdelgwad, M., M. Ewaiss, D. Sabry, W. A. Khalifa, Z. M. Altaib, and M. alhelf, "Comparative study on effect of mesenchymal stem cells and endothelial progenitor cells on treatment of experimental CCL4-induced liver fibrosis.", Archives of physiology and biochemistry, pp. 1-10, 2020. Abstract

We speculated impacts of BM-MSCs and UC-EPCs on reversal of hepatic injury induced by carbon tetrachloride (CCl4). Fifty adult rats were divided into five groups: control group, CCl4A group, CCl4B group, CCl4/BM-MSCs group and CCl4/UC-EPCs group. Blood samples were driven to measure concentration of albumin and ALT. Quantitative expression of HGF, TGF-β, MMP-2, and VEGF were assessed by PCR. Histological and immunohistochemistry examination of the liver tissue were performed. There was elevating albumin ( < .05) and reducing ALT ( < .05) concentrations in groups treated with BM-MSCs and UC-EPCs compared to untreated CCL4A&B groups. UC-EPCs treated group have significantly higher MMP-2 and VEGF ( < .01) genes expression than BM-MSCs treated group. Furthermore, UC-EPCs were more valuable than BMMSCs in increasing gene expression of HGF ( < .05) and immunohistochemistry of α-SMA and Ki-67 ( < .01). BM-MSCs have significantly lower TGF-β ( < .00) compared to UC-EPCs. This study highlighted on liver regeneration role of both UC-EPCs and BM-MSCs in liver fibrosis.

Abdelhamid, M., D. Sabry, D Gharib, and M. Abdel Gawad, "Statin treatment increases the circulating endothelial progenitor cells and improve endothelial function in patients with coronary artery diseases", EUROPEAN HEART JOURNAL, OXFORD UNIV PRESS, 35, pp. 1154-1154, 2014.
Abdelhamid, M., Y Sharaf, S. Bakhoum, D. Sabry, and A. Talaat, "Circulating EPCs predicts the occurrence of major adverse cardiac events and early adverse remodeling in patients with STEMI", EUROPEAN HEART JOURNAL, OXFORD UNIV PRESS, 35, pp. 651-651, 2014.
ABDELKADER, N. A. D. I. A. A., M. O. N. A. ZAKI, W. E. S. S. A. M. E. SAAD, G. Hamdy, and D. Sabry, "CLINICAL SIGNIFICANCE OF SERUM N-TERMINAL PRO C-TYPE NATRIURETIC PEPTIDE IN HEPATITIS C-RELATED CHRONIC LIVER DISEASES.", Journal of the Egyptian Society of Parasitology, vol. 45, issue 2, pp. 219-26, 2015.
Abdelsalam, A., L. Rashed, T. Salman, L. Hammad, and D. Sabry, "Molecular assessment of Vitamin D receptor polymorphism as a valid predictor to the response of Interferon/Ribavirin based therapy in Egyptian patients with Chronic Hepatitis C.", Journal of digestive diseases, 2016 Apr 29. Abstract

BACKGROUND/AIM: Egypt has the highest prevalence rate of Hepatitis C virus (HCV) infection around the globe, where, chronic hepatits C (CHC) is considered a major health problem. The standard treatment of CHC is combination therapy of pegylated interferon and ribavirin. Successful treatment and sustained virological response (SVR) are only achieved in 30% of patients. Major adverse effects and high cost of the treatment makes predicting the treatment output is an important approach. The aim of this study to find an association between Vitamin D concentration and vitamin D receptor (VDR) polymorphisms with achieving SVR.

METHODS: This is a case control study in which; 250 patients recruited and were divided into 3 groups (100 CHC patients who achieved SVR, 100 CHC patients who did not achieve SVR, and 50 apparently healthy individuals as control). Blood samples were collected to measure vitamin D concentration and 4 VDR polymorphisms (FokI, ApaI, TaqI, and BsmI) were detected using RFLP-PCR.

RESULTS: Non responders were found to have significantly low vitamin D concentration compared to responders and control groups. Concerning VDR polymorphisms, both FokI and TaqI polymorphisms were associated to successful treatment.

CONCLUSION: Vitamin D concentration, FokI, and TaqI may be considered as one of the predictors for the response of CHC patients to combination of pegylated interferon and ribvirin therapy. This article is protected by copyright. All rights reserved.

Aboud, H. M., M. O. Mahmoud, M. Abdeltawab Mohammed, M. Shafiq Awad, and D. Sabry, "Preparation and appraisal of self-assembled valsartan-loaded amalgamated Pluronic F127/Tween 80 polymeric micelles: Boosted cardioprotection regulation of Mhrt/Nrf2 and Trx1 pathways in cisplatin-induced cardiotoxicity.", Journal of drug targeting, vol. 28, issue 3, pp. 282-299, 2020. Abstract

This study aimed to develop valsartan (VAL)-loaded mixed micelles and investigate their cardioprotective potential and molecular mechanisms through Mhrt/Nrf2 and Trx1 pathways. VAL-loaded mixed micelles have not been elaborated and their impact on Mhrt/Nrf2 and Trx1 pathways has not been yet inspected. VAL-loaded mixed micelles were prepared, incorporating Pluronic F127 and Tween 80, adopting thin-film hydration method. The micelles were evaluated for drug entrapment efficiency, loading characteristics, particle size, morphology, drug release and micelles storage stability. The pharmacokinetic studies were explored in rats. Also, VAL suspension and mixed micelles were tested in cisplatin-induced cardiotoxicity in rats either pre to or simultaneously with cisplatin. RNA expression of lnc Mhrt and protein expression of Nrf2, Trx1, Ask1, AMPK and caspase 3, oxidative stress and cardiac injury markers besides tailed DNA% by comet assay were assessed. Pharmacokinetic studies evoked a 3.75-fold increase in oral bioavailability as compared with VAL suspension. Overall, treatment with VAL-loaded mixed micelles was superior to VAL suspension in decreasing oxidative stress and cardiac injury markers and restoring the abnormalities occurred in Mhrt/Nrf2 and Trx1 pathways. Thus, mixed micelles would be promising nanocarrier for the engineering of VAL with reinforced pharmacokinetics and cardioprotection characteristics.

Aboushady, I. M., Z. A. Salem, D. Sabry, and A. Mohamed, "Comparative study of the osteogenic potential of mesenchymal stem cells derived from different sources", J Clin Exp Dent. , vol. 10, issue 1, pp. e7-13, 2018. dentists_2018.pdf
AbuBakr, N., T. Haggag, D. Sabry, and Z. A. Salem, "Functional and histological evaluation of bone marrow stem cell-derived exosomes therapy on the submandibular salivary gland of diabetic Albino rats through TGFβ/ Smad3 signaling pathway.", Heliyon, vol. 6, issue 4, pp. e03789, 2020. Abstract

Background: To prevail over diabetes mellitus and its numerous complications, researchers are seeking new therapies. Exosomes are natural cargo of functional proteins and can be used as a therapeutic delivery of these molecules.

Objective: The aim of this study was to evaluate the effect of exosomes derived from bone marrow mesenchymal stem cells (BM-MSCs) as a therapeutic intervention in salivary gland diabetic complications.

Methods: Ten adult healthy male Albino rats, weighing about 150-200 g were grouped into 2 groups. Diabetic group I: consisted of 5 streptozotocin (STZ)-induced diabetic rats. Exosomes treated group II: consisted of 5 STZ-induced diabetic rats, each animal received a single injection of exosomes (100 μg/kg/dose suspended in 0.2 ml PBS) through the tail vein. All animals were sacrificed after 5 weeks from the beginning of the experiment. Submandibular salivary gland samples were excised and processed for histological, ultrastructural examination and PCR for TGFβ, Smad2 and Smad3. Blood glucose level was monitored weekly, salivary IgA and serum amylase were evaluated before and after diabetes induction and at the end of the experiment.

Results: Histological and ultrastructural results of the exosomes treated group were promising regarding the glandular and ductal elements with less fibrosis observed. Results of PCR supported the role of exosomes to inhibit the diabetic sequalae in salivary gland and its complications through inhibiting TGFβ and its related pathway via Smad2 and Smad3. Blood glucose levels were reduced. In addition, salivary glands' function was improved as evidenced by reduction in serum amylase and salivary IgA.

Conclusion: BM-MSC-derived exosomes could be a novel therapeutic strategy for diabetic complications involving salivary glands.

Abuohashish, H. M., M. M. Ahmed, D. Sabry, M. M. Khattab, and S. S. Al-Rejaie, "ACE-2/Ang1-7/Mas cascade mediates ACE inhibitor, captopril, protective effects in estrogen-deficient osteoporotic rats.", Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, vol. 92, pp. 58-68, 2017 May 19. Abstract

The local role of the renin angiotensin system (RAS) was documented recently beside its conventional systemic functions. Studies showed that the effector angiotensin II (AngII) alters bone health, while inhibition of the angiotensin converting enzyme (ACE-1) preserved these effects. The newly identified Ang1-7 exerts numerous beneficial effects opposing the AngII. Thus, the current study examines the role of Ang1-7 in mediating the osteo-preservative effects of ACEI (captopril) through the G-protein coupled Mas receptor using an ovariectomized (OVX) rat model of osteoporosis. 8 weeks after the surgical procedures, captopril was administered orally (40mgkg(-1) d(-1)), while the specific Mas receptor blocker (A-779) was delivered at infusion rate of 400ngkg(-1)min(-1) for 6 weeks. Bone metabolic markers were measured in serum and urine. Minerals concentrations were quantified in serum, urine and femoral bones by inductive coupled plasma mass spectroscopy (ICP-MS). Trabecular and cortical morphometry was analyzed in the right distal femurs using micro-CT. Finally, the expressions of RAS peptides, enzymes and receptors along with the receptor activator of NF-κB ligand (RANKL) and osteoprotegerin (OPG) were determined femurs heads. OVX animals markedly showed altered bone metabolism and mineralization along with disturbed bone micro-structure. Captopril significantly restored the metabolic bone bio-markers and corrected Ca(2+) and P values in urine and bones of estrogen deficient rats. Moreover, the trabecular and cortical morphometric features were repaired by captopril in OVX groups. Captopril also improved the expressions of ACE-2, Ang1-7, Mas and OPG, while abolished OVX-induced up-regulation of ACE-1, AngII, Ang type 1 receptor (AT1R) and RANKL. Inhibition of Ang1-7 cascade by A-779 significantly eradicated captopril protective effects on bone metabolism, mineralization and micro-structure. A-779 also restored OVX effects on RANKL expression and ACE-1/AngII/AT1R cascade and down-regulated OPG expression and ACE-2/Ang1-7/Mas pathway. In line with the clinical observations of the bone-preservative properties following ACE-1 inhibition, local activation of ACE-2/Ang1-7/Mas signaling and suppressed osteoclastogenesis seem responsible for the osteo-preservative effect of captopril, which could offers a potential therapeutic value in treatment of disabling bone and skeletal muscular diseases.

Abuohashish, H. M., M. M. Ahmed, D. Sabry, M. M. Khattab, and S. S. Al-Rejaie, "The ACE-2/Ang1-7/Mas cascade enhances bone structure and metabolism following angiotensin-II type 1 receptor blockade.", European journal of pharmacology, vol. 807, pp. 44-55, 2017 Apr 22. Abstract

The renin angiotensin system (RAS) regulates numerous systemic functions and is expressed locally in skeletal tissues. Angiotensin1-7 (Ang1-7) is a beneficial member of the RAS, and the therapeutic effects of a large number of angiotensin receptors blockers (ARBs) are mediated by an Ang1-7-dependent cascade. This study examines whether the reported osteo-preservative effects of losartan are mediated through the angiotensin converting enzyme2 (ACE-2)/Ang1-7/Mas pathway in ovariectomized (OVX) rats. Sham and OVX animals received losartan (10mg/kg/d p.o.) for 6 weeks. A specific Mas receptor blocker (A-779) was delivered via mini-osmotic pumps during the losartan treatment period. Serum and urine bone metabolism biomarker levels were measured. Bone trabecular and cortical morphometry were quantified in distal femurs, whereas mineral contents were estimated in ashed bones, serum and urine. Finally, the expression of RAS components, the receptor activator of NF-κB ligand (RANKL) and osteoprotegerin (OPG) was determined. Losartan significantly improved the elevated bone metabolism marker levels and altered trabecular and cortical structures in OVX animals, and restored normal urinary and skeletal mineral levels. Mas receptor inhibition significantly abolished all osteo-protective effects of losartan and enhanced the deleterious effects of OVX. Losartan enhanced OVX-induced up-regulation of ACE-1, AngII, angiotensin type 1 (AT1) receptor and RANKL expression, and increased ACE-2, Ang1-7, Mas and OPG expression in OVX animals. However, A-779 significantly eradicated the effects of losartan on RAS components and RANKL/OPG expression. Thus, Ang1-7 are involved in the osteo-preservative effects of losartan via Mas receptor, which may add therapeutic value to this well-known antihypertensive agent.

Abuohashish, H. M., M. M. Ahmed, D. Sabry, M. M. Khattab, and S. S. Al-Rejaie, "Angiotensin (1-7) ameliorates the structural and biochemical alterations of ovariectomy-induced osteoporosis in rats via activation of ACE-2/Mas receptor axis.", Scientific reports, vol. 7, issue 1, pp. 2293, 2017 May 23. Abstract

The local and systemic renin angiotensin system (RAS) influences the skeletal system micro-structure and metabolism. Studies suggested angiotensin 1-7 (Ang(1-7)) as the beneficial RAS molecule via Mas receptor activation. This study examines the function of Ang(1-7) in bone micro-architecture and metabolism in an ovariectomized (OVX) rodent model of osteoporosis. OVX rats showed structural and bone metabolic degeneration in parallel with suppressed expressions of the angiotensin converting enzyme-2 (ACE-2)/Ang(1-7)/Mas components. The infusion of Ang(1-7) markedly alleviated the altered bone metabolism and significantly enhanced both trabecular (metaphyseal) and cortical (metaphyseal-diaphyseal) morphometry. Urinary and bones minerals were also improved in OVX rats by Ang(1-7). The infusion of the heptapeptide enhanced ACE-2/Mas receptor expressions, while down-regulated AngII, ACE, and AngII type-1 receptor (AT1R) in OVX animals. Moreover, Ang(1-7) markedly improved osteoprotegerin (OPG) and lowered receptor activator NF-κB ligand (RANKL) expressions. The defensive properties of Ang(1-7) on bone metabolism, structure and minerals were considerably eradicated after blockage of Mas receptor with A-779. Ang(1-7)-induced up-regulated ACE-2/Ang(1-7)/Mas cascade and OPG expressions were abolished and the expressions of ACE/AngII/AT1R and RANKL were provoked by A-779. These findings shows for the first time the novel valuable therapeutic role of Ang(1-7) on bone health and metabolism through the ACE-2/Mas cascade.

Ahmed G Abdel Salam, Hazem M Ata, T. M. Salman, L. A. Rashed, D. Sabry, and M. F. Schaalan, "Potential therapeutic utility of mesenchymal stem cells in inflammatory bowel disease in mice", International immunopharmacology, vol. 22, issue 2, pp. 515-521, 2014.
Alaa El-Din, Y., D. Sabry, A. H. Abdelrahman, and S. Fathy, "Potential therapeutic effects of induced pluripotent stem cells on induced salivary gland cancer in experimental rats.", Biotechnic & histochemistry : official publication of the Biological Stain Commission, pp. 1-8, 2018 Oct 19. Abstract

Salivary gland neoplasms exhibit complex histopathology in a variety of tumor types and treatment options depend largely on the stage of the cancer. Induced pluripotent stem cells (iPS) have been investigated for treating induced salivary gland cancer and for restoring salivary gland function. We investigated iPS treatment for salivary gland cancer both in vitro and in vivo. For our study in vitro, we re-programmed human skin fibroblasts to form iPS cells using a plasmid containing Oct4, Sox2, L-MYC and LIN28. For our study in vivo, we used 30 white male albino rats divided into the following groups of 10: group 1 (control): rats were injected with phosphate-buffered saline (PBS), group 2 induced squamous cell carcinoma (SCC): rat submandibular glands were injected with squamous carcinoma cells (SCC), group 3 (induced SCC/iPS): SCC treated rats treated with 5 × 106 iPS cells. Submandibular glands from rats of all groups were examined histologically and real time PCR was performed for amylase, and COX I and COX II gene expression. We confirmed that submandibular gland specimens included tumor tissue before starting treatment with iPS. iPS treated cases exhibited regeneration of salivary glands, although minor degenerative and vascularization changes remained. The acinar cells regained their proper organization, but continued to exhibit abnormal activity including hyperchromatism. iPS cells may be useful for treating salivary gland carcinomas.

Ali, M. M., S. A. Khater, A. A. Fayed, D. Sabry, and S. F. Ibrahim, "Apoptotic endocrinal toxic effects of perchlorate in human placental cells.", Toxicology reports, vol. 8, pp. 863-870, 2021. Abstract

Background: Perchlorate is a strong oxidizing agent and has many adverse health effects. This study investigated the potential oxidative, apoptotic, and endocrinal toxic effects of perchlorate in human placenta-derived mesenchymal stem cells (HP-MSCs).

Methods: HP-MSCs were treated with two doses of perchlorate (5 and 15 μg/L) for three days. The perchlorate's effects were detected by histopathological examination, aromatase/CYP19 A1 activity, reactive oxygen species production (ROS), and Caspase-3 expression.

Results: The highest perchlorate concentration (15 μg/L) caused significant placental histopathological changes. The placental cell viability was significantly affected by a significant increase in ROS generation; caspase-3 expression, and a significant reduction of CYP 19 activity. Despite the slight induction effect of the lowest perchlorate concentration (5 μg/L) on caspase 3 expression, CYP 19 activity, and ROS generation, it did not affect placental cellular viability.

Conclusion: This study suggested that perchlorate could modulate aromatase activity and placental cytotoxicity. The continuous monitoring of the actual perchlorate exposure is needed and could be cost-effective.