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Roshdy, N. K., L. A. Rashed, D. Sabry, A. A. Hassouna, and F. M. Taha, Efficacy of mesenchymal stem cells in suppression of hepatocarcinorigenesis in rats: possible role of Wnt signaling, , 2011. Abstract
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Zaahkouk, A. M. S., M. T. Abdel Aziz, A. M. Rezq, H. M. Atta, H. H. Fouad, H. H. Ahmed, D. Sabry, and M. H. Yehia, "Efficacy of a novel water-soluble curcumin derivative versus sildenafil citrate in mediating erectile function", International journal of impotence research, vol. 27, issue 1, pp. 9-15, 2015.
Aziz, M. T. A., M. F. El-Asmar, T. Mostafa, H. Atta, M. A. A. Wassef, H. H. Fouad, N. K. Roshdy, L. A. Rashed, and D. Sabry, "Effects of nitric oxide synthase and heme oxygenase inducers and inhibitors on molecular signaling of erectile function", Journal of Clinical Biochemistry and Nutrition, vol. 37, no. 3: 日本酸化ストレス学会 JCBN 事務局, pp. 103–111, 2005. Abstract
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Aziz, A. E., M. Talaat, E. Asmer, M. Farid, T. Mostafa, H. Atta, S. Mahfouz, H. Fouad, L. Rashed, D. Sabry, et al., "Effects of Losartan, HO-1 Inducers or HO-1 Inhibitors on Erectile Signaling in Diabetic Rats", The journal of sexual medicine, vol. 6, no. 12: Wiley Online Library, pp. 3254–3264, 2009. Abstract
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Aziz, M. T. A., M. F. El-Asmar, A. M. Rezq, M. A. A. Wassef, H. Fouad, N. K. Roshy, H. H. Ahmed, L. A. Rashed, D. Sabry, F. M. Taha, et al., "Effects of a novel curcumin derivative on insulin synthesis and secretion in streptozotocin-treated rat pancreatic islets in vitro", Chinese medicine, vol. 9, issue 1, pp. 1-12, 2014.
Sabry, D., M. A. S. Al-Ghussein, G. Hamdy, A. Abul-Fotouh, T. Motawi, A. Y. El Kazaz, A. Eldemery, and M. Shaker, "Effect of vitamin D therapy on interleukin-6, visfatin, and hyaluronic acid levels in chronic hepatitis C Egyptian patients", Therapeutics and clinical risk management, vol. 11, pp. 279, 2015.
Sabry, D., A. Mohamed, M. Monir, and H. A. Ibrahim, "The Effect of Mesenchymal Stem Cells Derived Microvesicles on the Treatment of Experimental CCL4 Induced Liver Fibrosis in Rats.", International journal of stem cells, vol. 12, issue 3, pp. 400-409, 2019. Abstract

Background and Objectives: The release of microvesicles (MVs) from mesenchymal stem cells (MSCs) has been implicated in intercellular communication, and may contribute to beneficial paracrine effects of stem cell-based therapies. We investigated the effect of administration of MSC-MVs on the therapeutic potential of carbon tetrachloride (CCL) induced liver fibrosis in rats.

Methods: Our work included: isolation and further identification of bone marrow MSC-MVs by transmission electron microscopy (TEM). Liver fibrosis was induced in rats by CCl4 followed by injection of prepared MSC-MVs in injured rats. The effects of MSC-MVs were evaluated by biochemical analysis of liver functions, RNA gene expression quantitation for collagen-1, transforming growth factor (TGF-), interleukin-1 (IL-1), vascular endothelial growth factor (VEGF) by real time reverse transcription PCR (RT-PCR) techniques. Finally histopathological examination of the liver tissues was assessed for all studied groups.

Results: BM-MSC-MVs treated group showed significant increase in serum albumin levels, VEGF quantitative gene expression (p<0.05), while it showed a significant decrease in serum alanine transaminase (ALT) enzyme levels, quantitative gene expression of TGF-, collagen-1, IL-1 compared to CCL fibrotic group (p<0.05). Additionally, the histopathological assessment of the liver tissues of BM-MSC-MVs treated group showed marked decrease in the collagen deposition & improvement of histopathological picture in comparison with CCL fibrotic group.

Conclusions: Our study demonstrates that BM-MSC-MVs possess anti-fibrotic, anti-inflammatory, and pro-angiogenic properties which can promote the resolution of CCL induced liver fibrosis in rats.

MT1, A. A., K. HM, E. H. A, R. NK, H. A. A. T. A. W. I. F. Rashed LA, Sabry D, Hassouna AA, T. F, and A. WI., "Effect of mesenchymal stem cells and a novel curcumin derivative on Notch1 signaling in hepatoma cell line.", Biomed Res Int, 2013. Abstract

This study was conducted to evaluate the effect of mesenchymal stem cells (MSCs) and a novel curcumin derivative (NCD) on HepG2 cells (hepatoma cell line) and to investigate their effect on Notch1 signaling pathway target genes. HepG2 cells were divided into HepG2 control group, HepG2 cells treated with MSC conditioned medium (MSCs CM), HepG2 cells treated with a NCD, HepG2 cells treated with MSCs CM and NCD, and HepG2 cells treated with MSCs CM (CM of MSCs pretreated with a NCD). Expression of Notch1, Hes1, VEGF, and cyclin D1 was assessed by real-time, reverse transcription-polymerase chain reaction (RT-PCR) in HepG2 cells. In addition, HepG2 proliferation assay was performed in all groups. Notch1 and its target genes (Hes1 and cyclin D1) were downregulated in all treated groups with more suppressive effect in the groups treated with both MSCs and NCD. Also, treated HepG2 cells showed significant decrease in cell proliferation rate. These data suggest that modulation of Notch1 signaling pathway by MSCs and/or NCD can be considered as a therapeutic target in HCC.

Abdel Aziz, M. T., S. El-Haggar, T. Mostafa, H. Atta, H. Fouad, S. Mahfouz, L. Rashed, D. Sabry, A. Senbel, and G. A. Ali, "Effect of mesenchymal stem cell penile transplantation on erectile signaling of aged rats", Andrologia, vol. 42, no. 3: Wiley Online Library, pp. 187–192, 2010. Abstract
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Mazen I Naga, M. A. Amin, Dina A Algendy, ahmed i elbadry, Mai M Fawzi, Ayman R Foda, Serag M Esmat, D. Sabry, L. A. Rashed, and M. Kamal, "Effect of Hemochromatosis gene mutation on HCV response to treatment in the Egyptian population", HEPATOLOGY, WILEY-BLACKWELL, 60, pp. 931A-932A, 2014.
Abdel, A. M. T., F. M. El-Asmar, T. Mostafa, H. Atta, H. H. Fouad, N. K. Roshdy, L. A. Rashed, E. A. Obaia, D. A. Sabry, A. A. T. Abdel, et al., "Effect of hemin and carbon monoxide releasing molecule (CORM-3) on cGMP in rat penile tissue.", The journal of sexual medicine, vol. 5, no. 2, pp. 336–343, 2008. Abstract
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Sabry, D., S. Marzouk, R. Zakaria, H. A. Ibrahim, and M. Samir, "The effect of exosomes derived from mesenchymal stem cells in the treatment of induced type 1 diabetes mellitus in rats.", Biotechnology letters, 2020. Abstract

AIM: The aim of the current study was to evaluate the therapeutic and regenerative effects of MSCs derived exosomes in the treatment of type 1 DM and to compare its effects with MSCs themselves. The experiment was done on forty albino rats grouped as follows, group (1): Ten healthy rats, group (2): Ten induced type 1 DM rats, group (3): Ten induced type 1 DM rats received exosomes intraperitoneally, and group (4): Ten induced type 1 DM rats received MSCs intraperitoneally. Serum glucose and plasma insulin levels were assessed weekly. QRT-PCR was done to assess regeneration of pancreatic beta cells by measuring insulin, Pdx1, Smad2, Smad3 and TGFβ genes. Additionally, histopathological and immune-histochemical examinations were done to confirm pancreatic tissue regeneration.

RESULTS: Regarding the assessed genes (insulin, Pdx1, Smad2, Smad3 and Tgfβ) gene expression in MSCs treated group showed significant increase compared to diabetic group (p value < 0.001) and gene expression in exosomes treated group was increased significantly compared to diabetic and MSCs treated groups (p value < 0.001). Histopathological and immune-histochemical examination revealed regeneration of pancreatic islets in both treated groups.

CONCLUSION: MSCs Derived exosomes showed superior therapeutic and regenerative results than MSCs themselves.

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Salem, N., M. Y. Salem, M. M. Elmaghrabi, M. A. Elawady, M. A. Elawady, D. Sabry, A. Shamaa, A. - H. H. Elkasapy, N. Ibrhim, and A. E. Amir, "Does vitamin C have the ability to augment the therapeutic effect of bone marrow-derived mesenchymal stem cells on spinal cord injury?", Neural regeneration research, vol. 12, issue 12, pp. 2050-2058, 2017 Dec. Abstractneuralregenres.pdf

Methylprednisolone (MP) is currently the only drug confirmed to exhibit a neuroprotective effect on acute spinal cord injury (SCI). Vitamin C (VC) is a natural water-soluble antioxidant that exerts neuroprotective effects through eliminating free radical damage to nerve cells. Bone marrow mesenchymal stem cells (BMMSCs), as multipotent stem cells, are promising candidates in SCI repair. To evaluate the therapeutic effects of MP, VC and BMMSCs on traumatic SCI, 80 adult male rats were randomly divided into seven groups: control, SCI (SCI induction by weight-drop method), MP (SCI induction, followed by administration of 30 mg/kg MP via the tail vein, once every other 6 hours, for five times), VC (SCI induction, followed by intraperitoneal administration of 100 mg/kg VC once a day, for 28 days), MP + VC (SCI induction, followed by administration of MP and VC as the former), BMMSCs (SCI induction, followed by injection of 3 × 10BMMSCs at the injury site), and BMMSCs + VC (SCI induction, followed by BMMSCs injection and VC administration as the former). Locomotor recovery was assessed using the Basso Mouse Scale. Injured spinal cord tissue was evaluated using hematoxylin-eosin staining and immunohistochemical staining. Expression of transforming growth factor-beta, tumor necrosis factor-alpha, and matrix metalloproteinase-2 genes was determined using real-time quantitative PCR. BMMSCs intervention better promoted recovery of nerve function of rats with SCI, mitigated nerve cell damage, and decreased expression of transforming growth factor-beta, tumor necrosis factor-alpha, and matrix metalloproteinase-2 genes than MP and/or VC. More importantly, BMMSCs in combination with VC induced more obvious improvements. These results suggest that VC can enhance the neuroprotective effects of BMMSCs against SCI.

Baki, N. A. M., Z. O. Nawito, N. M. S. Abdelsalam, D. Sabry, H. Elashmawy, N. A. Seleem, A. A. A. - A. Taha, and M. E. Ghobashy, "Does Intra-Articular Injection of Platelet-Rich Plasma Have an Effect on Cartilage Thickness in Patients with Primary Knee Osteoarthritis?", Current rheumatology reviews, 2021. Abstract

OBJECTIVES: To determine the effect of intra-articular injection of platelet-rich plasma (PRP) in patients with primary knee osteoarthritis (OA) by clinical evaluation and ultrasonographic (US) assessment of cartilage thickness.

PATIENTS AND METHODS: A total of 100 patients with mild to severe primary knee OA using the Kellgren-Lawrence (K-L) grading scale were included and divided into two groups. Group I included 50 patients who were given two intra-articular knee injections of PRP, 1 week apart; Group II included 50 patients who received non-steroidal anti-inflammatory drugs (NSAIDs) and chondroprotective drugs. Functional assessment of all OA patients done using the basal WOMAC score, at 2 and 6 months.US assessment of femoral condylar cartilage thickness was conducted basally and at 6 months.

RESULTS: Improvement of WOMAC score was observed at 2 and 6 months in Group I following PRP injection compared to Group II (p values < 0.001), The improvement of WOMAC in Group I occurred in all severity degrees of OA (p < 0.001). Moreover, a significant increase in cartilage thickness at the intercondylar area (ICA) at 6 months relative to baseline assessment by US in Group I (p = 0.041) was found.

CONCLUSION: Treatment with PRP injections can reduce pain and improve knee function in patients with various degrees of articular degeneration. Further studies are needed to clarify the anabolic effect of PRP on the articular cartilage.

H, F., Raziky MS, Aziz RA, S. D, Aziz GM, Ewais M, and S. AR., "Dendritic cell co-stimulatory and co-inhibitory markers in chronic HCV: an Egyptian study.", World journal of gastroenterology: WJG, vol. 19, issue 43, pp. 7711-8, 2013.
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Nadia Abdelaaty Abdelkader, D. Sabry, Mohamed Al-Ghussein, Hany Mansour Dabbous, and E. H. Allam, "Correlation between Vitamin D Receptor and Monocyte Chemotactic Protein-1 Polymorphisms and Spontaneous Bacterial Peritonitis in Decompensated Liver Disease", Journal of Gastroenterology and Hepatology Research, vol. 4, issue 11, pp. 1815-1820, 2015.
Sayyed, H. G., A. Osama, N. K. Idriss, D. Sabry, A. S. Abdelrhim, and R. Bakry, "Comparison of the therapeutic effectiveness of human CD34(+) and rat bone marrow mesenchymal stem cells on improvement of experimental liver fibrosis in Wistar rats.", International journal of physiology, pathophysiology and pharmacology, vol. 8, issue 3, pp. 128-139, 2016. Abstract

BACKGROUND AND OBJECTIVE: Human umbilical cord blood (UCB) cells and bone marrow mesenchymal stem cells (BM-MSCs) have numerous advantages as grafts for cell transplantation. We hypothesized differing impacts of human UCB cells and rat BM-MSCs on reversal of hepatic injury and revival of liver function in carbon tetrachloride (CCl4)-induced liver fibrosis.

METHODS: Forty rats were divided into 4 groups; control group, CCl4 group, CCl4/CD34(+) group and CCl4/BM-MSCs group. Blood samples were driven from rats at 4, 8 and 12 weeks to measure serum concentration of albumin and alanine aminotransferase (ALT). Quantitative expression of collagen Iα, TGF-β, α-SMA, albumin, MMP-2, MMP-9 and TNF-α were assessed by polymerase chain reaction. Histopathological examination of the liver tissue was performed. GFP labeled cells were detected in groups injected with stem cells.

RESULTS: Regarding liver function, CD34(+) were more efficient than BM-MSCs in elevating albumin (P<0.05) and reducing ALT (P<0.05) concentrations. Concerning gene expression, CD34(+) were more effective than BM-MSCs in reducing gene expressions of collagen Iα (P<0.01), TGF-β1 (P<0.01) and α-SMA (P<0.01). Both CD34(+) and BM-MSCs have the same efficacy in reducing TNF-α (P<0.001 and P<0.01, respectively). Furthermore, CD34(+) were more valuable than BM-MSCs in increasing gene expression of albumin (P<0.05) and MMP-9 (P<0.01).

CONCLUSION: Taken together; human UCB CD34(+) stem cells were more efficient in improvement of experimental liver injury than BM-MSCs. This study highlighted an important role of human UCB CD34(+) stem cells in liver fibrosis therapy.

Abdelgwad, M., M. Ewaiss, D. Sabry, W. A. Khalifa, Z. M. Altaib, and M. alhelf, "Comparative study on effect of mesenchymal stem cells and endothelial progenitor cells on treatment of experimental CCL4-induced liver fibrosis.", Archives of physiology and biochemistry, pp. 1-10, 2020. Abstract

We speculated impacts of BM-MSCs and UC-EPCs on reversal of hepatic injury induced by carbon tetrachloride (CCl4). Fifty adult rats were divided into five groups: control group, CCl4A group, CCl4B group, CCl4/BM-MSCs group and CCl4/UC-EPCs group. Blood samples were driven to measure concentration of albumin and ALT. Quantitative expression of HGF, TGF-β, MMP-2, and VEGF were assessed by PCR. Histological and immunohistochemistry examination of the liver tissue were performed. There was elevating albumin ( < .05) and reducing ALT ( < .05) concentrations in groups treated with BM-MSCs and UC-EPCs compared to untreated CCL4A&B groups. UC-EPCs treated group have significantly higher MMP-2 and VEGF ( < .01) genes expression than BM-MSCs treated group. Furthermore, UC-EPCs were more valuable than BMMSCs in increasing gene expression of HGF ( < .05) and immunohistochemistry of α-SMA and Ki-67 ( < .01). BM-MSCs have significantly lower TGF-β ( < .00) compared to UC-EPCs. This study highlighted on liver regeneration role of both UC-EPCs and BM-MSCs in liver fibrosis.

Aboushady, I. M., Z. A. Salem, D. Sabry, and A. Mohamed, "Comparative study of the osteogenic potential of mesenchymal stem cells derived from different sources", J Clin Exp Dent. , vol. 10, issue 1, pp. e7-13, 2018. dentists_2018.pdf
El-Tantawy, W. H., D. Sabry, and E. N. Abd Al Haleem, "Comparative study of antifibrotic activity of some magnesium-containing supplements on experimental liver toxicity. Molecular study.", Drug and chemical toxicology, pp. 1-10, 2016 May 5. Abstract

INTRODUCTION: Liver fibrosis is the excessive accumulation of extracellular matrix (ECM) proteins including collagen that occurs in most types of chronic liver diseases. This study aimed to investigate and compare the therapeutic efficacy of different magnesium (Mg)-containing supplements (formulations A, B, and C) on carbon tetrachloride (CCl4)-induced liver fibrosis in rats.

METHODS: Liver fibrosis was induced by intraperitoneal injection of rats with CCl4 (1:1 in olive oil, 2 mL/kg, three times/week) for 4 weeks, and then rats were orally treated with different Mg-containing supplements (formulations A, B, and C) once daily for another one month. Liver fibrosis was quantified by evaluation of expressions of Collagen I, transforming growth factor β-1 (TGFβ1), platelet-derived growth factor-C (PDGF-C), nuclear factor kappa-β (NF-κβ), and measurement of hepatic collagen (hydroxyproline) level. Also, malondialdehyde (MDA), nitric oxide (NO), glutathione (GSH) level, superoxide dismutase (SOD), and glutathione-S-transferase (GST) activities were estimated.

RESULTS: CCl4 administration significantly elevated expressions of the studied genes, hepatic hydroxyproline, MDA, and NO levels and caused depletion of GSH level, decreased SOD, and GST activities when compared with those of their corresponding control, p < 0.05. All magnesium supplements significantly inhibited expressions of the studied genes and attenuated the hepatic hydroxyproline level as compared with those of CCl4-treated group; p < 0.05; for NF-κβ, the highest inhibition was by formulations B and C. Regarding Collagen I, TGFβ1, and hepatic hydroxyproline content, the highest inhibition was by Formulation C, and Formulation A revealed highest inhibition for PDGF-C. All magnesium supplements revealed normalization of oxidant and antioxidants parameters. Histopathological examination supports the biochemical and molecular findings.

CONCLUSION: Mg supplements were effective in the treatment of hepatic CCl4-induced fibrosis-rat model.

El-Tantawy, W. H., D. Sabry, and E. N. Abd Al Haleem, "Comparative study of antifibrotic activity of some magnesium-containing supplements on experimental liver toxicity. Molecular study.", Drug and chemical toxicology, vol. 40, issue 1, pp. 47-56, 2017 Jan. Abstract

INTRODUCTION: Liver fibrosis is the excessive accumulation of extracellular matrix (ECM) proteins including collagen that occurs in most types of chronic liver diseases. This study aimed to investigate and compare the therapeutic efficacy of different magnesium (Mg)-containing supplements (formulations A, B, and C) on carbon tetrachloride (CCl4)-induced liver fibrosis in rats.

METHODS: Liver fibrosis was induced by intraperitoneal injection of rats with CCl4 (1:1 in olive oil, 2 mL/kg, three times/week) for 4 weeks, and then rats were orally treated with different Mg-containing supplements (formulations A, B, and C) once daily for another one month. Liver fibrosis was quantified by evaluation of expressions of Collagen I, transforming growth factor β-1 (TGFβ1), platelet-derived growth factor-C (PDGF-C), nuclear factor kappa-β (NF-κβ), and measurement of hepatic collagen (hydroxyproline) level. Also, malondialdehyde (MDA), nitric oxide (NO), glutathione (GSH) level, superoxide dismutase (SOD), and glutathione-S-transferase (GST) activities were estimated.

RESULTS: CCl4 administration significantly elevated expressions of the studied genes, hepatic hydroxyproline, MDA, and NO levels and caused depletion of GSH level, decreased SOD, and GST activities when compared with those of their corresponding control, p < 0.05. All magnesium supplements significantly inhibited expressions of the studied genes and attenuated the hepatic hydroxyproline level as compared with those of CCl4-treated group; p < 0.05; for NF-κβ, the highest inhibition was by formulations B and C. Regarding Collagen I, TGFβ1, and hepatic hydroxyproline content, the highest inhibition was by Formulation C, and Formulation A revealed highest inhibition for PDGF-C. All magnesium supplements revealed normalization of oxidant and antioxidants parameters. Histopathological examination supports the biochemical and molecular findings.

CONCLUSION: Mg supplements were effective in the treatment of hepatic CCl4-induced fibrosis-rat model.

Sabry, D., A. Osama, N Idriss, and M. Magdy, "Comparative study between the effects of human CD34+ and rat bone marrow mesenchymal stem cells on amelioration of CCL4 induced liver fibrosis", FEBS JOURNAL, France WILEY-BLACKWELL, 281, pp. 222-222, 2014.
Shaimaa M Masloub, Mohamed H Elmalahy, D. Sabry, Wael S Mohamed, and S. H. Ahmed, "Comparative evaluation of PLGA nanoparticle delivery system for 5-fluorouracil and curcumin on squamous cell carcinoma", Archives of oral biology, vol. 64, pp. 1-10, 2016.
Sabry, D., Olfat Noh, and M. Samir, "Comparative Evaluation for Potential Differentiation of Endothelial Progenitor Cells and Mesenchymal Stem Cells into Endothelial-Like Cells.", International journal of stem cells, vol. 9, issue 1, pp. 44-52, 2016 May 30. Abstract

Understanding the mechanisms of vascular remodeling could lead to more effective treatments for ischemic conditions. We aimed to compare between the abilities of both human Wharton jelly derived mesenchymal stem cells (hMSCs) and human cord blood endothelial progenitor cells (hEPCs) and CD34⁺ to induce angiogenesis in vitro. hMSCs, hEPCs, and CD34⁺ were isolated from human umbilical cord blood using microbead (MiniMacs). The cells characterization was assessed by flow cytometry following culture and real-time PCR for vascular endothelial growth factor receptor 2 (VEGFR2) and von Willebrand factor (vWF) to prove stem cells differentiation. The study revealed successful isolation of hEPCs, CD34⁺, and hMSCs. The hMSCs were identified by gaining CD29⁺ and CD44⁺ using FACS analysis. The hEPCs were identified by having CD133⁺, CD34⁺, and KDR. The potential ability of hEPCs and CD34⁺ to differentiate into endothelial-like cells was more than hMSCs. This finding was assessed morphologically in culture and by higher significant VEGFR2 and vWF genes expression (p<0.05) in differentiated hEPCs and CD34⁺ compared to differentiated hMSCs. hEPCs and CD34⁺ differentiation into endothelial-like cells were much better than that of hMSCs.

A, F., S. D, A. R, K. M, Allah SA, Marzouk S, A. M, Abd El Aziz R, El Badri A, K. H, et al., "Comparative diagnostic study of biomarkers using FibroMax™ and pathology for prediction of liver steatosis in patients with chronic hepatitis C virus infection: an Egyptian study.", Int J Gen Med, vol. 12, issue 6, pp. 127-34, 2013.