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Salama, M. K., D. Sabry, MA Al-Ghussein, R. Ahmed, S. A. Allah, F. M. Taha, W. Fathy, M. S. Wadie, M. Nabih, A. Abul-Fotouh, et al., "Molecular detection of monocyte chemotactic protein-1 polymorphism in spontaneous bacterial peritonitis patients", World journal of gastroenterology: WJG, vol. 20, issue 33, pp. 11793-11799, 2014.
Abdelsalam, A., L. Rashed, T. Salman, L. Hammad, and D. Sabry, "Molecular assessment of Vitamin D receptor polymorphism as a valid predictor to the response of Interferon/Ribavirin based therapy in Egyptian patients with Chronic Hepatitis C.", Journal of digestive diseases, 2016 Apr 29. Abstract

BACKGROUND/AIM: Egypt has the highest prevalence rate of Hepatitis C virus (HCV) infection around the globe, where, chronic hepatits C (CHC) is considered a major health problem. The standard treatment of CHC is combination therapy of pegylated interferon and ribavirin. Successful treatment and sustained virological response (SVR) are only achieved in 30% of patients. Major adverse effects and high cost of the treatment makes predicting the treatment output is an important approach. The aim of this study to find an association between Vitamin D concentration and vitamin D receptor (VDR) polymorphisms with achieving SVR.

METHODS: This is a case control study in which; 250 patients recruited and were divided into 3 groups (100 CHC patients who achieved SVR, 100 CHC patients who did not achieve SVR, and 50 apparently healthy individuals as control). Blood samples were collected to measure vitamin D concentration and 4 VDR polymorphisms (FokI, ApaI, TaqI, and BsmI) were detected using RFLP-PCR.

RESULTS: Non responders were found to have significantly low vitamin D concentration compared to responders and control groups. Concerning VDR polymorphisms, both FokI and TaqI polymorphisms were associated to successful treatment.

CONCLUSION: Vitamin D concentration, FokI, and TaqI may be considered as one of the predictors for the response of CHC patients to combination of pegylated interferon and ribvirin therapy. This article is protected by copyright. All rights reserved.

Ebrahim, N., E. Ehsan, E. A. Ghany, D. Sabry, and A. Shamaa, "Mesenchymal stem cells transplantation attenuates experimentally induced brain injury after neonatal hypoxia by different two routes of administrations ", Biocell, vol. 43, issue 1, pp. 21-28, 2019.
Ebrahim, N., I. A. Ahmed, N. I. Hussien, A. A. Dessouky, A. S. Farid, A. M. Elshazly, O. Mostafa, W. B. E. Gazzar, S. M. Sorour, Y. Seleem, et al., "Mesenchymal Stem Cell-Derived Exosomes Ameliorated Diabetic Nephropathy by Autophagy Induction through the mTOR Signaling Pathway.", Cells, vol. 7, issue 12, 2018 Nov 22. Abstract

BACKGROUND: Diabetic nephropathy (DN) is a serious complication of diabetes mellitus and a common cause of end-stage renal disease. Autophagy has a defensive role against kidney damage caused by hyperglycemia. Mesenchymal stem cell (MSC)-derived exosomes are currently considered as a new promising therapy for chronic renal injury. However, the renal-protective mechanism of exosomes on DN is not completely understood. We examined the potential role of MSC-derived exosomes for enhancement of autophagy activity and their effect on DN. In our study, we used five groups of rats: control; DN; DN treated with exosomes; DN treated with 3-methyladenine (3-MA) and chloroquine (inhibitors of autophagy); and DN treated with 3-methyladenine (3-MA), chloroquine, and exosome groups. We assessed renal function, morphology, and fibrosis. Moreover, ratios of the autophagy markers mechanistic target of rapamycin (mTOR), Beclin-1, light chain-3 (LC3-II), and LC3-II/LC3-I were detected. Additionally, electron microscopy was used for detection of autophagosomes.

RESULTS: Exosomes markedly improved renal function and showed histological restoration of renal tissues, with significant increase of LC3 and Beclin-1, and significant decrease of mTOR and fibrotic marker expression in renal tissue. All previous effects were partially abolished by the autophagy inhibitors chloroquine and 3-MA.

CONCLUSION: We conclude that autophagy induction by exosomes could attenuate DN in a rat model of streptozotocin-induced diabetes mellitus.

Lobna A Aly, H. E. - Menoufy, Rehab Tarek Elsharkawy, Mona Z Zaghloul, and D. Sabry, "Maternal chronic oral infection with periodontitis and pericoronitis as a possible risk factor for preeclampsia in Egyptian pregnant women (microbiological and serological study)", Future Dental Journal, vol. 1, issue 1, pp. 23-32, 2015.
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Sabry, D., O. O. Abdelaleem, E. M. Hefzy, A. A. Ibrahim, T. I. Ahmed, E. A. Hassan, N. D. Abdel-Hameed, and M. A. F. Khalil, "Interplay Between Helicobacter pylori Infection, Interleukin-11, and Leukemia Inhibitory Factor in Gastric Cancer Among Egyptian Patients.", Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research, vol. 38, issue 11, pp. 517-525, 2018 Nov. Abstract

Helicobacter pylori is a ubiquitous Gram-negative bacterium, that is responsible for gastric mucosal inflammation. It is the most common risk factor for gastric cancer (GC). The current study aimed to investigate the association between interleukin-11 (IL-11) and leukemia inhibitory factor (LIF) levels among H. pylori-infected Egyptian patients with gastritis and GC. One hundred forty-seven patients with gastric lesions were endoscopically biopsied and assessed using rapid urease test and immunohistochemistry. Quantitative real-time polymerase chain reaction was done for the detection of H. pylori load in gastric biopsies and detection of LIF as well as IL-11 relative gene expression. The mean values of H. pylori load, LIF, and IL-11 were significantly elevated in GC patients compared to gastritis group (P < 0.0001). A positive significant correlation was detected between mucosal levels of LIF, IL-11, and H. pylori load in both groups. Both LIF and IL-11 had the same pattern of expression in gastric tissues with different types of gastritis and different types and grades of gastric carcinoma. This report could clarify the molecular events associated with the immune response against H. pylori infection and H. pylori-associated pathology. Therefore, development of immunotherapy strategies against H. pylori-induced cytokines becomes inevitable.

Mostafa-Hedeab, G., D. Sabry, G. M. Abdelaziz, M. Ewaiss, N. Adli, and W. Fathy, "Influence of Vitamin D Receptor Gene Polymorphisms on Response to Pegylated Interferon in Chronic Hepatitis B Egyptian Patients ", Reports of Biochemistry & Molecular Biology , vol. 6, issue 2, pp. 186-196, 2018. rbmb.pdf
Fouad, R., M. Abdo, H. G. E. deen, D. Sabry, Mira Atef, R. Ahmed, and N. Zayed, "Influence of delta virus infection on the virologic status in Egyptian patients with chronic Hepatitis B virus genotype D", J Med Virol., vol. 88, issue 5, pp. 837-42, 2016.
Omar, R. F., R. Ahmed, D. Sabry, Mahmoud Abdel Alim, Mira Atef, and N. Zayed, "Influence of Delta Virus Infection on the Clinical Spectrum, Serologic and Virologic Status in Chronic Hepatitis B Virus Genotype D", HEPATOLOGY, WILEY-BLACKWELL, 60, pp. 971A-972A, 2014.
Abdelfattah, D. S., R. Sayed, G. Mostafa-Hedeab, and H. H. M. Ali, "In Vitro Characterization and Evaluation of Silver Nanoparticles Cytotoxicity on Human “Liver and Breast” Cancer Cells Versus Normal Melanocytes ", The Egyptian journal of histology, vol. 42, issue 3, pp. 755-766, 2019.
Abdel Aziz, M. T., D. Sabry, M. A. S. Al-Ghussein, A. M. A. Soliman, and A. A. Hassan, "IN VITRO AND IN VIVO EVALUATION ACTIVITIES OF EACH MESENCHYMAL STEM CELLS, DOXORUBICIN AND LPS OR COMBINED ON BREAST CARCINOMA", WORLD JOURNAL OF PHARMACY AND PHARMACEUTICAL SCIENCES, vol. 3, issue 8, pp. 157-176, 2014.
Esmat, G., M. E. Raziky, A. Elsharkawy, D. Sabry, A. Mohamed Hassany, A. Ahmed, N. Assem, M. El Kassas, and W. D, "Impact of vitamin D supplementation on sustained virological response in chronic hepatitis C genotype 4 patients treated by pegylated interferon/ribavirin", Journal of Interferon & Cytokine Research, vol. 35, issue 1, pp. 49-54, 2015.
H
Ebrahim, N., O. Mostafa, R. E. El Dosoky, I. A. Ahmed, A. S. Saad, A. Mostafa, D. Sabry, K. A. Ibrahim, and A. S. Farid, "Human mesenchymal stem cell-derived extracellular vesicles/estrogen combined therapy safely ameliorates experimentally induced intrauterine adhesions in a female rat model.", Stem cell research & therapy, vol. 9, issue 1, pp. 175, 2018 Jun 28. Abstract

BACKGROUND: Mesenchymal stem cells (MSCs) have diverse functions in regulating injury and inflammation through the secretion of extracellular vesicles (EVs).

METHODS: In this study, we investigated the systemic administration of extracellular vesicles derived from human umbilical cord mesenchymal stem cells (UCMSCs-EVs) as a therapeutic agent for intrauterine adhesions (IUAs) caused by endometrial injury. Additionally, we investigated the therapeutic impact of both UCMSCs-EVs and estrogen either separately or in combination in a rat model. The inflammation, vascularization, proliferation, and extent of fibrosis were assessed by a histopathological and immunohistochemical assessment using transforming growth factor (TGF)-β as a fibrotic marker and vascular endothelial growth factor (VEGF) as a vascular marker. Additionally, quantitative real-time polymerase chain reaction (qRT-PCR) was used to analyze the expression of tumor necrosis factor (TNF)-α, interleukin (IL)-1, IL-6 (inflammatory cytokines), CD140b (a marker of endometrial stem cells), and RUNX2 (an antifibrotic factor). Finally, Western blotting was used to evaluate collagen I and β-actin expression.

RESULTS: The therapeutic groups treated with either UCMSCs-EVs alone or combined with estrogen exhibited a significant decrease in inflammation and fibrosis (TNF-α, TGF-β, IL-1, IL-6, RUNX2, and collagen-I) as well as a significant decrease in vascularization (VEGF) compared with the untreated rats with IUAs. The most significant results were obtained in animals with IUAs that received a combined therapy of UCMSCs-EVs and estrogen.

CONCLUSIONS: We conclude that the synergistic action of human UCMSCs-EVs combined with estrogen provides a highly effective alternative regenerative agent in IUA treatment.

Mokbel, A. N., O. S. El Tookhy, A. A. Shamaa, L. A. Rashed, D. Sabry, and A. M. Elsayed, "Homing and reparative effect of intra-articular injection of autologus mesenchymal stem cells in osteoarthritic animal model", BMC musculoskeletal disorders, vol. 12, no. 1: BioMed Central Ltd, pp. 259, 2011. Abstract
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Esmat, G. E., W. A. Akel, R. A. A. Aziz, A. Al Sayed Taha, D. Sabry, L. A. Rashed, A. Mostafa, A. Y. El Kazaz, and S. H. Ahmed, "Hepatitis C Viral Kinetic Changes in a Retrospective Cohort Study of Chronic Hepatitis C Virus Egyptian Patients on Pegylated Interferon and Ribavirin Therapy.", Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research, vol. 36, issue 3, pp. 149-58, 2016 Mar. Abstract

The aim of this study was to determine the relative importance of the kinetics of antiviral response compared to baseline host and virological factors for predicting treatment outcome. A retrospective analysis of 285 chronic hepatitis C virus (HCV) patients, encompassing genotypes 4 treated with peginterferon alpha-2a and ribavirin, was performed. Baseline characteristics were compared across HCV genotypes and pretreatment factors associated with rapid virological response (RVR) were identified. The relative significance of RVR compared to other baseline factors for predicting sustained virological response was analyzed by multiple logistic regression analysis. Ninety-seven percent of the patients harbored HCV genotype 4a patients. The positive predictive value (PPV) of RVR for end-of-treatment response (ETR) was 88% and of early virological response (EVR) was 85%, which means that achievement of both RVR and EVR is a good positive predictive factor of response. The negative predictive value (NPV) of RVR for ETR was low and equals 26.77%, which means that approximately two-thirds of patients were able to achieve ETR despite not experiencing RVR, which means RVR is a bad negative predictive factor of response. The NPV of EVR for ETR was high and equals 90%, which means that only 10% of patients were able to achieve an ETR despite not experiencing EVR, which explains that EVR is a very good negative predictive factor of response. In univariate logistic regression analysis, which included the following: female gender, alanine aminotransferase, aspartate transaminase, α-fetoprotein, baseline HCV-RNA levels, grade of activity, stage of fibrosis, and positive HCV-RNA, by polymerase chain reaction at week 4, none of the previous factors was a significant independent factor of failure of response to treatment. The current study demonstrated that a viremia at week 4 has a good PPV, but it has a very low NPV. The NPV of EVR was more robust for ETR (90%). EVR is regarded as a robust indicator of treatment outcome, and a 12-week stopping rule for patients is strongly evident.

Abdel Aziz, M. T., T. Mostafa, H. Atta, O. Kamal, M. Kamel, H. Hosni, L. Rashed, D. Sabry, and F. Waheed, "Heme oxygenase enzyme activity in seminal plasma of oligoasthenoteratozoospermic males with varicocele", Andrologia, vol. 42, no. 4: Wiley Online Library, pp. 236–241, 2010. Abstract
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El-Rifaie, A. - A. A., D. Sabry, R. W. Doss, M. A. Kamal, and D. M. Abd El Hassib, "Heme oxygenase and iron status in exosomes of psoriasis patients.", Archives of dermatological research, 2018 Aug 13. Abstract

Psoriasis is an autoimmune skin disease characterized by hyperproliferation of keratinocytes due to interplay between keratinocytes and immune cells. Iron status plays an important role in modifying the function of the immune system. Heme oxygenase (HO), heme-degrading enzyme, plays important role in protective response to oxidative cellular stress. We aimed in this study to map the iron status and HO levels and declare the role HO enzyme in iron homeostasis and immune-modulation in psoriasis. Fifty-one patients with psoriasis and 50 age- and sex-matched healthy controls were enrolled in this study. 5 mL blood sample was withdrawn from each subject. Hepcidin, iron soluble transferring receptor (sTfR), and total iron binding capacity (TIBC) were estimated using ELISA technique and, HO-1 gene level was detected using RT-PCR (reverse transcription-polymerase chain reaction). Iron levels, TIBC, and hepcidin were significantly lower in cases compared to controls. On the contrary, sTfR and HO-1 were significantly over-expressed in cases compared to controls (p < 0.05 in all). HO-1 expression negatively correlated with PASI score and disease extent (%) (r = - 0.614-, p = 0.001; r = - 0.807-, p = 0.001 respectively). There were no significant associations between HO-1 expression and iron, TIBC, hepcidin, sTfR levels (p > 0.05 in all). Iron supplements for the patients with psoriasis are important to maintain haematopoiesis. The induction of HO-1 might have be a promising approach for the treatment of psoriasis through antioxidant ability, immunomodulatory role as well as its role in heme synthesis.

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Hasan, E. M., R. A. Abd Al Aziz, D. Sabry, S. K. Darweesh, H. A. Badary, A. Elsharkawy, M. M. Abouelkhair, and A. Yosry, "Genetic Variants in nicotinamide-N-methyltransferase (NNMT) gene are related to the stage of non-alcoholic fatty liver disease diagnosed by controlled attenuation parameter (CAP)-fibroscan.", Journal of gastrointestinal and liver diseases : JGLD, vol. 27, issue 3, pp. 265-272, 2018 Sep. Abstract

BACKGROUND AND AIMS: Various genetic polymorphisms play a key-role in the pathogenesis of NAFLD and progression to NASH with fibrosis to cirrhosis. We aimed to study the association between single-nucleotide polymorphisms (SNPs) in NNMT gene, namely rs694539 and the development of different stages of NAFLD diagnosed by controlled attenuation parameter (CAP) of FibroScan Echosens®.

METHODS: Transient elastography (FibroScan®) with controlled attenuation parameter (CAP) measurement was performed in 81 NAFLD patients (35 of them with liver biopsy) and 80 non-NAFLD controls. The accuracy of CAP and FibroScan for the detection and quantification of hepatic steatosis/fibrosis, respectively, was assessed based on liver biopsy aspect. Genetic variants of NNMT gene rs694539 were analyzed using a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).

RESULTS: According to BMI (kg/m2), among the patients, 17 (21%) were overweight, 56 (69.1%) obese, and 8 (9.9%) morbidly obese. CAP and FibroScan diagnosed steatosis/fibrosis correlated significantly with liver biopsy. There was a significant association between polymorphisms of rs694539-NNMT gene and NAFLD presence and stages. The mutant type (AA-genotype) was found in 33% NAFLD patients versus 1.2% controls (P<0.001), whereas the wild type (GG-genotype) was present in 21% versus 63.8% controls (P<0.001). Moreover, the AA-genotype significantly correlated with the steatosis degree by CAP but not the fibrosis degree by FibroScan. Multivariate regression analysis of all the independent risk factors showed non-significant correlations with the degree of steatosis on CAP. However, by using a stepwise approach, waist circumference showed significance as an independent predictor of NAFLD.

CONCLUSIONS: Polymorphisms in rs694539-NNMT gene (mutant AA-genotype) could be a genetic risk factor for developing NAFLD and NASH (indicating susceptibility for progression and complications). Individuals with wild type (GG-genotype) are at less risk of NAFLD development. CAP and FibroScan efficiently diagnosed steatosis and fibrosis.

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AbuBakr, N., T. Haggag, D. Sabry, and Z. A. Salem, "Functional and histological evaluation of bone marrow stem cell-derived exosomes therapy on the submandibular salivary gland of diabetic Albino rats through TGFβ/ Smad3 signaling pathway.", Heliyon, vol. 6, issue 4, pp. e03789, 2020. Abstract

Background: To prevail over diabetes mellitus and its numerous complications, researchers are seeking new therapies. Exosomes are natural cargo of functional proteins and can be used as a therapeutic delivery of these molecules.

Objective: The aim of this study was to evaluate the effect of exosomes derived from bone marrow mesenchymal stem cells (BM-MSCs) as a therapeutic intervention in salivary gland diabetic complications.

Methods: Ten adult healthy male Albino rats, weighing about 150-200 g were grouped into 2 groups. Diabetic group I: consisted of 5 streptozotocin (STZ)-induced diabetic rats. Exosomes treated group II: consisted of 5 STZ-induced diabetic rats, each animal received a single injection of exosomes (100 μg/kg/dose suspended in 0.2 ml PBS) through the tail vein. All animals were sacrificed after 5 weeks from the beginning of the experiment. Submandibular salivary gland samples were excised and processed for histological, ultrastructural examination and PCR for TGFβ, Smad2 and Smad3. Blood glucose level was monitored weekly, salivary IgA and serum amylase were evaluated before and after diabetes induction and at the end of the experiment.

Results: Histological and ultrastructural results of the exosomes treated group were promising regarding the glandular and ductal elements with less fibrosis observed. Results of PCR supported the role of exosomes to inhibit the diabetic sequalae in salivary gland and its complications through inhibiting TGFβ and its related pathway via Smad2 and Smad3. Blood glucose levels were reduced. In addition, salivary glands' function was improved as evidenced by reduction in serum amylase and salivary IgA.

Conclusion: BM-MSC-derived exosomes could be a novel therapeutic strategy for diabetic complications involving salivary glands.

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Sherif, I. O., N. H. Al-Shaalan, and D. Sabry, "Extract Alleviates Methotrexate-Induced Renal Injury: New Impact on PI3K/Akt/mTOR Signaling and MALAT1 Expression.", Biomolecules, vol. 9, issue 11, 2019. Abstract

Renal injury induced by the chemotherapeutic agent methotrexate (MTX) is a serious adverse effect that has limited its use in the treatment of various clinical conditions. The antioxidant activity of extract (GB) was reported to mitigate renal injury induced by MTX. Our research was conducted to examine the nephroprotective role of GB versus MTX-induced renal injury for the first time through its impact on the regulation of phosphatidylinositol 3-kinase/protein kinase B/ mammalian target of rapamycin (PI3K/Akt/mTOR) signaling together with the renal level of TGF-β mRNA and long non-coding RNA-metastasis-associated lung adenocarcinoma transcript-1 (MALAT1) expression. A group of adult rats was intraperitoneally (ip) injected with MTX 20 mg/kg as a single dose to induce kidney injury (MTX group). The other group of rats was orally administered with GB 60 mg/kg every day for 10 days (GB+ MTX group). The MTX increased the serum creatinine and urea levels, renal TGF-β mRNA and MALAT1 expression, in addition to dysregulation of the PI3K/Akt/mTOR signaling when compared with normal control rats that received saline only (NC group). Moreover, renal damage was reported histopathologically in the MTX group. The GB ameliorated the renal injury induced by MTX and reversed the changes of these biochemical analyses. The involvement of PI3K/Akt/mTOR signaling and downregulation of TGF-β mRNA and MALAT1 renal expressions were firstly reported in the nephroprotective molecular mechanism of GB versus MTX-induced renal injury.

Motawi, T. M. K., S. A. EL-Maraghy, D. Sabry, and N. A. Mehana, "The expression of long non coding RNA genes is associated with expression with polymorphisms of HULC rs7763881 and MALAT1 rs619586 in hepatocellular carcinoma and HBV Egyptian patients.", Journal of cellular biochemistry, vol. 120, issue 9, pp. 14645-14656, 2019. Abstract

Long noncoding RNAs (lncRNAs), highly upregulated liver cancer (HULC), metastasis-associated lung adenocarcinoma transcript 1 (MALAT1), lncRNA-AF085935, and lncRNA-uc003wbd have been implicated in hepatocellular carcinoma (HCC). Single-nucleotide polymorphism (SNP) in HULC and MALAT1 are associated with HCC susceptibility. However, association between these SNPs and lncRNA-AF085935 and lncRNA-uc003wbd expression and their potential clinical value in differentiating HCC from both hepatitis B virus (HBV)-infected Egyptian patients and the healthy specimens have not been explored yet. In the present study, SNPs rs7763881 in HULC and rs619586 in MALAT1 were genotyped in 70 HBV-positive HCC, 70 HBV patients, and 70 healthy controls in Egyptian population and the level of serum lncRNA-AF085935 and lncRNA-uc003wbd of all the subjects was assayed by quantitative real-time polymerase chain reaction. HULC rs7763881 AC/CC genotype was significantly associated with decreased HCC risk. Similarly, AG/GG of MALAT1 rs619586 was associated with decreased HCC risk with a borderline significance. Serum lncRNA-AF085935 and lncRNA-uc003wbd levels were upregulated in HBV-positive HCC and HBV patients vs controls and discriminated these groups by receiver operating characteristic analysis. Patients carrying AC/CC genotype of rs7763881 and AG/GG of rs619586 had lower serum lncRNA-AF085935 and lncRNA-uc003wbd levels compared with AA genotype. In conclusion, genetic variants of lncRNA HULC and lncRNA MALAT1 are associated with the decreased susceptibility to HCC in HBV-persistent carriers and are correlated with serum lncRNA-AF085935 and lncRNAuc003wbd levels, two potential noninvasive diagnostic biomarkers for HCC.

Aziza, A. E. M. T., A. E. E. A. Nabia, A. E. M. Hamidc, D. Sabrya, H. M. Attaa, L. A. Raheda, A. Shamaae, and S. Mahfouzf, "Endothelial progenitor cells regenerate infracted myocardium with neovascularisation development.", Journal of Advanced Research, vol. 6, issue 2, pp. 133–144, 2015. Abstract

We achieved possibility of isolation, characterization human umbilical cord blood endothelial progenitor cells (EPCs), examination potency of EPCs to form new blood vessels and differentiation into cardiomyoctes in canines with acute myocardial infarction (AMI). EPCs were separated and cultured from umbilical cord blood. Their phenotypes were confirmed by uptake of double stains dioctadecyl tetramethylindocarbocyanine-labeled acetylated LDL and FITC-labeled Ulex europaeus agglutinin 1 (DILDL-UEA-1). EPCs of cord blood were counted. Human VEGFR-2 and eNOS from the cultured EPCs were assessed by qPCR. Human EPCs was transplanted intramyocardially in canines with AMI. ECG and cardiac enzymes (CK-MB and Troponin I) were measured to assess severity of cellular damage. Histopathology was done to assess neovascularisation. Immunostaining was done to detect EPCs transdifferentiation into cardiomyocytes in peri-infarct cardiac tissue. qPCR for human genes (hVEGFR-2, and eNOS) was done to assess homing and angiogenic function of transplanted EPCs. Cultured human cord blood exhibited an increased number of EPCs and significant high expression of hVEGFR-2 and eNOS genes in the culture cells. Histopathology showed increased neovascularization and immunostaining showed presence of EPCs newly differentiated into cardiomyocyte-like cells. Our findings suggested that hEPCs can mediate angiogenesis and differentiate into cardiomyoctes in canines with AMI.

Abbreviations
CTO, chronic total occlusion; CAG, coronary angiography; AMI, acute myocardial infarction; DILDL-FITC labeled UEA-11, 1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine-labeled acetylated LDL (DiLDL,) and FITC-labeled Ulex europaeus agglutinin-1; MVD, multivessel disease; CFU, colony forming unit

Mostafa, T., L. A. Rashed, N. Nabil, H. Fouad, D. Sabry, and D. M. El-Saied, "Endothelial nitric oxide synthase gene polymorphism relationship with semen parameters and oxidative stress in infertile oligoasthenoteratozoospermic men", Urology, vol. 85, issue 5, pp. 1058-1061, 2015.
Lobna A Aly, H. E. - Menoufy, Hesham S Sadeq, A. Ragae, and D. Sabry, "Efficiency of systemic versus intralesional bone marrow-derived stem cells in regeneration of oral mucosa after induction of formocresol induced ulcers in dogs", Dental research journal, vol. 11, issue 2, pp. 212, 2014.
Abdelgawad, L. M., M. M. Abd El-Hamed, D. Sabry, and M. Abdelgwad, "Efficacy of Photobiomodulation and Metformin on Diabetic Cell Line of Human Periodontal Ligament Stem Cells through Pathway.", Reports of biochemistry & molecular biology, vol. 10, issue 1, pp. 30-40, 2021. Abstract

Background: Diabetes mellitus (DM) is a metabolic disorder resulting from hyperglycemia. Hyperglycemia contributes to oxidative stress, and the release of advanced glycation end products (AGEs) further promotes disease pathogenesis. Uncontrolled diabetes reflects great oral complications and affects human oral health. So, the present study aimed to assess the effects of photobiomodulation therapy (PBMT) and Metformin on proliferation and viability of human periodontal ligament stem cells (HPDLSCs) cultured in high glucose medium.

Methods: HPDLSCs were collected, isolated, and characterized and then divided into eight groups. Addition of extra glucose to diabetic groups 24 hours before cell irradiations. Metformin was added to half of the diabetic groups. Cells were irradiated with 808 nm diode laser 24, 48 hours. Cell viability was analyzed with MTT assay 24 hours post-irradiation to detect cell viability in each group. Real-time (PCR) was used to evaluate gene expression of and and the effect of PBMT on Pathway. ELISA reader was used to evaluating cell viability through (ROS, TNF-α, IL-10) protein levels after cell irradiation.

Results: Photobiomodulation at 1, 2, and 3 J/cm2 combined with metformin significantly promoted diabetic cell lines of HPDLSCs viability (in MTT assay and ELISA reader of ROS, TNF-α, IL-10 results) and gene expression of , and levels (p< 0.05).

Conclusion: photobiomodulation with 3 J/cm combined with metformin enhanced proliferation and viability of diabetic cell lines of HPDLSCs and thus could improve differentiation and function of diabetic cell lines of HPDLSCs with minimum side effects.