@article {228887,
	title = {Effect of Diacerein on HOTAIR/IL-6/STAT3, Wnt/β-Catenin and TLR-4/NF-κB/TNF-α axes in colon carcinogenesis.},
	journal = {Environmental toxicology and pharmacology},
	volume = {95},
	year = {2022},
	pages = {103943},
	abstract = {<p>Colorectal cancer (CRC) is a common malignancy with high mortality and poor prognosis. Diacerein (DIA) is an anti-inflammatory used for treatment of osteoarthritis. We delineated some underlying molecular mechanisms of DIA{\textquoteright}s anti-carcinogenic effect in CRC using in vivo and in vitro models. Human Caco-2 cells were treated with DIA followed by MTT and Annexin V assays and CRC was experimentally induced using 1,2-dimethylhydrazine. DIA (50~mg/kg/day, orally) was administrated for 8 weeks. The MTT assay confirmed cytotoxic effect of DIA in vitro and Annexin V confirmed its apoptotic effect. DIA resulted in regression of tumour lesions with reduced colonic TLR4, NF-κB and TNF-α protein levels and down-regulated VEGF expression, confirming anti-angiogenic impact. DIA triggered caspase-3 expression and regulated Wnt/β-Catenin pathway, by apparently interrupting the IL-6/STAT3/ lncRNA HOTAIR axis. In conclusion, DIA disrupted IL-6/STAT3/ lncRNA HOTAIR axis which could offer an effective therapeutic strategy for the management of CRC.</p>
},
	author = {Eisa, Nada H and Eman Said and Khodir, Ahmed E and Dina Sabry and Ebrahim, Hasnaa Ali and Elsherbini, Dalia Mahmoud Abdelmonem and Altemani, Reem and Alnasser, Dania Mohammed and Elsherbiny, Nehal M and Mohamed El-Sherbiny}
}
