@article {151389, title = {XRCC1 Gene Polymorphisms and miR-21 Expression in Patients with Colorectal Carcinoma.}, journal = {The Eurasian journal of medicine}, volume = {49}, year = {2017}, month = {2017 Jun}, pages = {132-136}, abstract = {

OBJECTIVE: The objectives of this study were to evaluate the impact of two X-ray repair cross complementing 1 (XRCC1) gene polymorphisms (Arg194Trp and Arg399Gln) on the risk of development of colorectal cancer (CRC) and to assess the expression levels of microRNA-21 (miR-21) in CRC patients.

MATERIALS AND METHODS: A case-control cross sectional study was conducted on 50 CRC patients and 50 cancer-free subjects. DNA and miR-21 were extracted from whole blood samples. The expression levels of the XRCC1 polymorphisms and miR-21 were assessed by real-time PCR in all subjects of the study.

RESULTS: Genotype analysis revealed a significant association between CRC risk and both the Arg194Trp genotype (OR=11.407, 95\% CI=4.039-32.221, p\<0.001) and the Arg399Gln genotype (OR=3.778, 95\% CI= 1.6-8.919, p=0.002). The expression levels of circulating miR-21 were able to detect CRC cases significantly (p=0.022) with a sensitivity of 82\% and a specificity of 56\% (Area under the curve (AUC)=0.633) but were unable to distinguish between early and late cases (AJCC classification) (p=0.194).

CONCLUSION: The XRCC1 Arg194Trp and Arg399Gln polymorphisms both confer high susceptibility for the development of CRC. Circulating miR-21 expression levels are a potentially diagnostic non-invasive genetic marker of CRC.

}, issn = {1308-8734}, doi = {10.5152/eurasianjmed.2017.17021}, attachments = {https://scholar.cu.edu.eg/sites/default/files/yasmine/files/final_reprint_dr._naglaa_md_article_132-136.pdf}, author = {Hanan Fouad and Dina Sabry and Morsi, Heba and Shehab, Hany and Abuzaid, Naglaa F} }