@article {120457, title = {Comparison of the therapeutic effectiveness of human CD34(+) and rat bone marrow mesenchymal stem cells on improvement of experimental liver fibrosis in Wistar rats.}, journal = {International journal of physiology, pathophysiology and pharmacology}, volume = {8}, year = {2016}, month = {2016}, pages = {128-139}, abstract = {

BACKGROUND AND OBJECTIVE: Human umbilical cord blood (UCB) cells and bone marrow mesenchymal stem cells (BM-MSCs) have numerous advantages as grafts for cell transplantation. We hypothesized differing impacts of human UCB cells and rat BM-MSCs on reversal of hepatic injury and revival of liver function in carbon tetrachloride (CCl4)-induced liver fibrosis.

METHODS: Forty rats were divided into 4 groups; control group, CCl4 group, CCl4/CD34(+) group and CCl4/BM-MSCs group. Blood samples were driven from rats at 4, 8 and 12 weeks to measure serum concentration of albumin and alanine aminotransferase (ALT). Quantitative expression of collagen Iα, TGF-β, α-SMA, albumin, MMP-2, MMP-9 and TNF-α were assessed by polymerase chain reaction. Histopathological examination of the liver tissue was performed. GFP labeled cells were detected in groups injected with stem cells.

RESULTS: Regarding liver function, CD34(+) were more efficient than BM-MSCs in elevating albumin (P\<0.05) and reducing ALT (P\<0.05) concentrations. Concerning gene expression, CD34(+) were more effective than BM-MSCs in reducing gene expressions of collagen Iα (P\<0.01), TGF-β1 (P\<0.01) and α-SMA (P\<0.01). Both CD34(+) and BM-MSCs have the same efficacy in reducing TNF-α (P\<0.001 and P\<0.01, respectively). Furthermore, CD34(+) were more valuable than BM-MSCs in increasing gene expression of albumin (P\<0.05) and MMP-9 (P\<0.01).

CONCLUSION: Taken together; human UCB CD34(+) stem cells were more efficient in improvement of experimental liver injury than BM-MSCs. This study highlighted an important role of human UCB CD34(+) stem cells in liver fibrosis therapy.

}, author = {Sayyed, Hayam G and Osama, Amany and Idriss, Naglaa K and Dina Sabry and Abdelrhim, Azza S and Bakry, Rania} }