@article {117235, title = {SIRT-1expression is associated with expression of NANOG in patients with colorectal adenocarcinoma.}, journal = {Cancer biomarkers : section A of Disease markers}, volume = {17}, year = {2016}, month = {2016 Jun 24}, pages = {155-163}, abstract = {

AIMS: The study aimed to investigate the quantitative expression of NANOG, p38 α , NCF2, ELF and TGF-β genes in patients with colorectal adenocarcinoma, adenoma and normal colonic tissue and their correlation with SIRT-1 protein level expression.

METHOD: This study enrolled one hundred sixty seven patients; group A: 87 patients with colonoscopic findings of no adenoma or adenocarcinoma and group B: 80 patients with colorectal mass. Consecutive colonoscopic examinations were conducted, and tissue samples were taken from the colonic lesions/masses. Total RNA was isolated and mRNA expression level of NANOG, mitogen activated p38α , Neutrophil Cytosol Factor 2 (NCF2), Embryonic Liver Fodrin (ELF) and Transforming Growth Factor Beta (TGF-β) genes were quantified by qRT-PCR. Sirt-1 protein expression level was assessed by quantitative western blot.

RESULTS: There were significantly high level of mRNA transcripts expression of the genes studied in patients with adenocarcinoma and adenoma compared with normal tissue (P value \< 0.01), NANOG, NCF2, ELF and TGF-β at a cut of \> 0.314, \> 0.392, 0.349 and 0.333 respectively showed sensitivity (96.5\%, 98.8\%, 95.3\%, 98.8\%) and specificity of (95.3\%, 92.6\%, 89.5\%, 93.8\%) respectively in diagnosing colonic adenocarcinoma. Sirt-1 protein level was significantly highly expressed in colorectal adenocarcinoma compared to normal and adenoma colonic tissue and positively correlated with NANOG.

CONCLUSION: Over expression of NANOG, p38α , NCF2, ELF and TGF-β genes in both cases of adenocarcinoma and adenoma could have a diagnostic value. SIRT-1 and NANOG are high correlated biological markers for diagnosis and prognosis follow up in patients with adenocarcinoma.

}, issn = {1875-8592}, doi = {10.3233/CBM-160626}, author = {Osama, Amany and Dina Sabry and Hassany, Sahar M and Abdelmoneim, Soha Saoud and Sabry, Abeer} }