@article {244410, title = {Assessment of interleukin-18 gene polymorphism and serum levels in cutaneous lichen planus.}, journal = {European journal of medical research}, volume = {29}, year = {2024}, pages = {345}, abstract = {

BACKGROUND: Lichen planus (LP) is a chronic inflammatory disease with uncertain etiology. Interleukin-18 (IL-18) is an interferon gamma (INFγ) inducing agent. It is a pro-inflammatory cytokine that was found to play a role in the pathogenesis of some autoimmune disorders.

MATERIAL AND METHODS: This study included 50 patients with classic cutaneous lichen planus (CLP) and 50 healthy volunteers serving as controls. Venous blood samples were withdrawn from the study subjects under complete aseptic precautions. Blood samples were examined for single nucleotide polymorphisms (SNPs) of IL-18 gene at promoter -137(G/C) and -656 (G/T) using polymerase chain reaction (PCR) and IL-18 level was assessed using enzyme linked immunosorbent assay (ELISA).

RESULTS: The mean level of IL-18 was significantly higher in CLP patients (31.63 {\textpm} 4.90) compared to control subjects (13.95 {\textpm} 6.82). Significantly high levels of IL-18 were found among patients with diabetes, hypertension (p \< 0.01 in both). HCV positive patients and patients with both OLP and CLP also expressed higher levels of IL-18. Genotypic and allelic distribution at position -137(G/C) showed that the genotype GG was present at significantly higher frequency in cases (58\%) compared to controls (28.0\%). On the other hand the CC genotype at position -137 was significantly higher in the controls (28\%) as compared to CLP cases (6\%). Polymorphism of IL-18 at position -656(G/T) showed no significant difference between cases and controls. No significant difference could be detected in IL-18 level between different genotypic variants at position -137(G/C) and -656(G/T).

CONCLUSION: IL-18 may play important role in pathogenesis of LP. Elevated IL-18 levels could be part of the pro-inflammatory autoimmune process in LP. The presence of OLP, HCV, diabetes and hypertension is associated with higher production of IL-18. IL-18 promotor region -137(G/C) polymorphism might be a factor that increase the risk of development of lichen planus in Egyptian patients.

}, author = {Reham William Doss and Abdel-Aziz El-Rifaie and Roshdy, Anton Nasr and Dina Sabry} } @article {244404, title = {Bone marrow aspirate concentrate - A novel approach to alter the course of temporomandibular joint osteoarthritis (a clinical study).}, journal = {Journal of stomatology, oral and maxillofacial surgery}, volume = {125}, year = {2024}, pages = {101644}, abstract = {

INTRODUCTION: TMJ OA is characterized by severe osteocartilaginous degradation of the joint structure resulting in severe deterioration of both joint function as well as joint structure. bone marrow aspirate concentrate (BMAC) gained wide acceptance as an auspicious addition for regenerative medicine as it is confirmed to be a rich source of pluripotent mesenchymal stem cells and growth factors that produce promising relief of clinical symptoms with significant repair of the joint structure. Thus, the study aims at assessing the efficacy of bone marrow aspirate concentrate (BMAC) as a treatment modality for TMJ osteoarthritis and compare its efficacy with that of hyaluronic acid (HA).

METHODS: 24 patients were included in the present study and divided into 12 patients in each group. Joint arthrocentesis was performed to all patients followed by intra-articular BMAC injection in Group I. While Group II received HA acid injection RESULTS: A trend towards long term joint repair at 12 and 18 months follow up period was observed in the bone marrow aspirate concentrate (BMAC) group as a therapeutic modality for TMJ OA by providing necessary growth factors and anti-inflammatories that impedes the progression of the osteoarthritic degeneration. On the contrary to the viscosupplementary action of hyaluronic acid (HA) that showed relapse of patients conditions.

CONCLUSION: Bone marrow aspirate concentrate (BMAC) is able to reverse the degenerative effects of TMJ OA however,further studies are mandatory with larger population and longer follow-up time.

}, author = {Fayed, Heba Mohamed and Khairy, Maggie A and ELdahshan, Dina and Dina Sabry and Ahmed, Waheed A} } @article {244407, title = {Exosomal miR-122, miR-128, miR-200, miR-298, and miR-342 as novel diagnostic biomarkers in NAFL/NASH: Impact of LPS/TLR-4/FoxO3 pathway.}, journal = {Archiv der Pharmazie}, volume = {357}, year = {2024}, pages = {e2300631}, abstract = {

Nonalcoholic fatty liver disease (NAFLD) is a common liver disorder affecting a quarter of the global residents. Progression of NAFL into nonalcoholic steatohepatitis (NASH) may cause cirrhosis, liver cancer, and failure. Gut microbiota imbalance causes microbial components translocation into the circulation, triggering liver inflammation and NASH-related fibrosis. MicroRNAs (miRNAs) regulate gene expression via repressing target genes. Exosomal miRNAs are diagnostic and prognostic biomarkers for NAFL and NASH liver damage. Our work investigated the role of the gut microbiota in NAFLD pathogenesis via the lipopolysaccharide/toll-like receptor 4/Forkhead box protein O3 (LPS/TLR-4/FoxO3) pathway and certain miRNAs as noninvasive biomarkers for NAFL or its development to NASH. miRNA~expression levels were measured using quantitative reverse transcription polymerase chain reaction (qRT-PCR) in 50 NAFL patients, 50 NASH patients, and 50 normal controls. Plasma LPS, TLR-4, adiponectin, peroxisome proliferator-activated receptor γ (PPAR-γ), and FoxO3 concentrations were measured using enzyme-linked immunosorbent assay (ELISA). In NAFL and NASH patients, miR-122, miR-128, FoxO3, TLR-4, LPS, and PPAR-γ were upregulated while miR-200, miR-298, miR-342, and adiponectin were downregulated compared with the normal~control. The examined miRNAs might distinguish NAFL and NASH patients from the normal control~using receiver operating characteristic analysis. Our study is the first to examine these miRNAs in NAFLD. Our findings imply that these are potentially promising biomarkers for noninvasive early NAFL diagnosis and NASH progression. Understanding the LPS/TLR-4/FoxO3 pathway involvement in NAFL/NASH pathogenesis may aid disease management.

}, author = {Samy, Ahmed M and Kandeil, Mohamed A and Dina Sabry and AA Abdel-Ghany and Mahmoud, Mohamed O} } @article {244409, title = {From NAFLD to NASH: Understanding the spectrum of non-alcoholic liver diseases and their consequences.}, journal = {Heliyon}, volume = {10}, year = {2024}, pages = {e30387}, abstract = {

Non-alcoholic fatty liver disease (NAFLD) has become one of the most frequent chronic liver diseases worldwide in recent decades. Metabolic diseases like excessive blood glucose, central obesity, dyslipidemia, hypertension, and liver function abnormalities cause NAFLD. NAFLD significantly increases the likelihood of liver cancer, heart disease, and mortality, making it a leading cause of liver transplants. Non-alcoholic steatohepatitis (NASH) is a more advanced form of the disease that causes scarring and inflammation of the liver over time and can ultimately result in cirrhosis and hepatocellular carcinoma. In this review, we briefly discuss NAFLD{\textquoteright}s pathogenic mechanisms, their progression into NASH and afterward to NASH-related cirrhosis. It also covers disease epidemiology, metabolic mechanisms, glucose and lipid metabolism in the liver, macrophage dysfunction, bile acid toxicity, and liver stellate cell stimulation. Additionally, we consider the contribution of intestinal microbiota, genetics, epigenetics, and ecological factors to fibrosis progression and hepatocellular carcinoma risk in NAFLD and NASH patients.

}, author = {Samy, Ahmed M and Kandeil, Mohamed A and Dina Sabry and AA Abdel-Ghany and Mahmoud, Mohamed O} } @article {244411, title = {The Role of Intra-Articular Delivery of BM-MSCs-Derived Exosomes in Improving Osteoarthritis: Implication of // Axis.}, journal = {Discovery medicine}, volume = {36}, year = {2024}, pages = {1420-1429}, abstract = {

BACKGROUND: Bone marrow-derived mesenchymal stem cells (BM-MSCs) have recently attracted great attention due to their crucial anti-inflammatory and regenerative properties. This work aims to examine the curative impact of intra-articular injection of BM-MSCs-derived exosomes in ameliorating osteoarthritis (OA) progression in rats and to explore the interaction between circular RNA of Yes-associated protein 1 () and microRNA-21 () in the rat knee joints.

METHODOLOGY: Gene expression , , toll-like receptor-7 (), aggrecan, and collagen type II were evaluated by quantitative reverse transcription polymerase chain reaction (qRT-PCR) in the rat articular tissues. In addition, the Enzyme-linked immunosorbent assay (ELISA) technique was used to estimate the level of the inflammatory markers interleukin 4 (IL-4), interleukin 10 (IL-10), interleukin 1β (IL-1β), and tumor necrosis factor-alpha (TNF-α); and the oxidative markers glutathione (GSH), malondialdehyde (MDA) and total reactive oxygen species (ROS). Histopathological examination using Hematoxylin and Eosin (H\&E) staining of the rat articular tissue was also performed along with an estimation of the articular cartilage thickness.

RESULTS: Our results showed that BM-MSCs-derived exosomes significantly elevated gene expression level ( \< 0.05) along with subsequent downregulation of and ( \< 0.05). These effects impacted the inflammatory milieu of rat articular surfaces, where there was a significant reduction ( \< 0.05) of the pro-inflammatory and oxidative markers with significantly increased production of the anti-inflammatory and antioxidative markers ( \< 0.05). Marked elevation in aggrecan and collagen type II gene expression was also found in the treated groups ( \< 0.05).

CONCLUSION: Those data suggest that BM-MSCs-derived exosomes have a crucial role in mitigating OA symptoms and pathology progression and might be regarded as an effective as well as acceptable treatment option for OA.

}, author = {Saad El-Din, Shimaa and BasmaEmad Aboulhoda and Hassouna, Amira and Shakweer, Marwa M and Alghamdi, Mansour A and Essam, Doha and Mohamed Essam, and AlRakaf, Nawaf A and Elzahed, Hanaa M and Selmy, Asmaa and Dina Sabry and Mekawy, Dina M} } @article {244408, title = {Silymarin and MSC-exosomes ameliorate thioacetamide-evoked renal fibrosis by inhibiting TGF-β/SMAD pathway in rats.}, journal = {Molecular biology reports}, volume = {51}, year = {2024}, pages = {529}, abstract = {

BACKGROUND: TGF-β1 and SMAD3 are particularly pathogenic in the progression of renal fibrosis.

AIM: This study aimed to evaluate the kidney protective potentials of silymarin (SM) and exosomes of mesenchymal stem cells against the nephrotoxin thioacetamide (TAA) in rats.

METHODS: 32 female rats were randomly assigned into four groups: the control group, the TAA group, the TAA + SM group, and the TAA + Exosomes group. The kidney homogenates from all groups were examined for expression levels of TGF-β receptors I and II using real-time PCR, expression levels of collagen type I and CTGF proteins using ELISA, and the expression levels of nuclear SMAD2/3/4, cytoplasmic SMAD2/3, and cytoplasmic SMAD4 proteins using the western blot technique.

RESULTS: Compared to the control group, the injection of TAA resulted in a significant increase in serum levels of urea and creatinine, gene expression levels of TβRI and TβRII, protein expression levels of both collagen I and CTGF proteins, cytoplasmic SMAD2/3 complex, and nuclear SMAD2/3/4 (p-value \< 0.0001), with significantly decreased levels of the co-SMAD partner, SMAD4 (p-value \< 0.0001). Those effects were reversed considerably in both treatment groups, with the superiority of the exosomal treatment regarding the SMAD proteins and the expression levels of the TβRI gene, collagen I, and CTGF proteins returning to near-control values (p-value \> 0.05).

CONCLUSION: Using in vitro and in vivo experimental approaches, the research discovered a reno-protective role of silymarin and exosomes of BM-MSCs after thioacetamide-induced renal fibrosis in rats, with the advantage of exosomes.

}, author = {Mekawy, Dina Mohamed and Dina Sabry and Rania Mohamed Sabry and Abozeid, Naglaa F} } @article {244406, title = {TLR4, IgA and EpCAM Expression in Colorectal Cancer and Their Possible Association with Microbiota as a Pathogenic Factor; An Immunohistochemical and Genetic Study.}, journal = {Asian Pacific journal of cancer prevention : APJCP}, volume = {25}, year = {2024}, pages = {627-636}, abstract = {

BACKGROUND: The pathogenesis of inflammatory bowel disease (IBD) and colorectal cancer (CRC) is thought to be related to immune response against gut microbiota. TLR4, IgA, and EpCAM have a role in intestinal local immune response and their altered expression related to both IBD and CRC. Lipopolysaccharide (LPS) is the main activator of TLR4. The objective of this study is to evaluate the possible role of intestinal microbiota in the pathogenesis of IBD and CRC through expression of TLR4, IgA and EpCAM.

METHODS: One hundred five cases were divided into (Group 1/ Control: 10 sections of normal colonic mucosa, Group 2/CRC: 51 cases, Group 3/IBD: 44 cases). Immunohistochemistry for TLR4, IgA, and EpCAM was done. LPS was assessed in all groups. TLR4 gene and protein expression were assessed in colorectal cancer cell line by RT-PCR and immunocytochemistry.

RESULTS: There was a significant correlation between TLR4 and tumor grade (P value 0.003 and 0.01 respectively). A significant correlation was found between IgA expression and T stage (P value 0.02) and between EpCAM expression and histologic type (P value 0.02). In comparison of CRC patients to controls; there was a statistically significant different expression of TLR4 positivity, IgA positivity and EpCAM (P value \<0.001, 0.004, \<0.001 respectively). Patients with CRC were compared to colitis patients and there was a statistically significant different expression of IgA positivity and EpCAM expression (P value \<0.001). There was significant higher expression of TLR4 in CRC cell line than the fibroblast by both PCR and immunocytochemistry (P-value: 0.003 and 0.024 respectively). LPS level in CRC patients was significantly higher than the control and IBD groups (P values \<0.001 and \<0.001 respectively).

CONCLUSION: TLR4, IgA, EpCAM expression in both CRC and IBD might be related to the pathogenic role of microbiota and could represent potential prevention modalities and therapeutic targets.

}, author = {Hafez, Fatma S and Shakweer, Marwa M and Dina Sabry and Tawfik, Amany M and El-Beah, Shaimaa Mohsen and Elsheshtawy, Nadia M and Shash, Lobna S and Salama, Doaa E A and Gaballah, Ahmed} } @article {228909, title = {Bone Marrow-Derived Mesenchymal Stem Cells and -Secretase Inhibitor Treatments Suppress Amyloid-25-35-Induced Cognitive Impairment in Rat Dams and Cortical Degeneration in Offspring.}, journal = {Stem cells international}, volume = {2023}, year = {2023}, pages = {2690949}, abstract = {

Alzheimer{\textquoteright}s disease (AD) is the most frequent cause of age-related neurodegeneration and ensuing cognitive impairment. Progressive deposition of extracellular amyloid beta (A) aggregates (plaques) and intracellular hyperphosphorylated Tau protein (p-Tau) are the core pathological markers of AD but may precede clinical symptoms by many years, presenting a therapeutic window of opportunity. Females are more frequently afflicted by AD than males, necessitating evaluation of novel treatments for the female population. The current study examined the protective efficacies of intravenous bone marrow-derived mesenchymal stem cells (BM-MSCs) and oral gamma-secretase inhibitor-953 (GSI-953) during pregnancy on cognitive impairment in rat dams and neurodegeneration in offspring induced by intracerebroventricular injection of A25-35 prior to pregnancy. The A25-35 (AD) group exhibited significant ( \< 0.001) impairments in the Y-maze and novel object recognition test performance prior to conception. Histological analysis of the offspring cortex revealed substantial dendritic shrinkage and activation of microglial cells, while neurochemical analysis demonstrated significant increases in the proinflammatory cytokine interleukin-1 (IL-1) and tumor necrosis factor- (TNF-). In contrast, BM-MSC or GSI-953 treatment of dams following A25-35 injection significantly ( \< 0.001) reduced the number and size of activated microglial cells, markedly increased dendrite length, and reversed proinflammatory cytokine elevations in offspring. Moreover, BM-MSC or GSI-953 treatment reversed the A25-35-induced amyloid precursor protein and p-Tau elevations in the offspring brain; these changes were accompanied by upregulation of the brain-derived neurotrophic factor and downregulation of glycogen synthase kinase-3 in the serum and brain. Treatment with BM-MSCs or GSI-953 also reversed A25-35-induced elevations in different gene expressions in the neonatal cortex. Finally, treatment of dams with BM-MSCs or GSI-953 prevented the A25-35-induced disruption of newborn brain development. Thus, BM-MSC and GSI-953 treatments have broad-spectrum effects against A25-35-induced brain pathology, including the suppression of neural inflammation, restoration of developmental plasticity, and promotion of neurotrophic signaling.

}, author = {Gaber, Asmaa and Elbakry, Ahlam M and Aljarari, Rabab M and Jaber, Fatima A and Yasser A. Khadrawy and Dina Sabry and Abo-ELeneen, Rasha E and Osama M. Ahmed} } @article {228912, title = {Circular SERPINA3 and its target microRNA-944 as potential biomarkers in hepatitis C virus-induced hepatocellular carcinoma in Egyptian population.}, journal = {Non-coding RNA research}, volume = {8}, year = {2023}, pages = {401-412}, abstract = {

BACKGROUND: The most prevalent cancer in Egypt is hepatocellular carcinoma (HCC) mainly due to the infection with the hepatitis C virus. So it is critical to find sensitive biomarkers for early diagnosis of HCC and avoid post-operation tumor recurrence. Therefore, this research was designed to demonstrate the circSERPINA3 role in the regulation of microRNA-944 gene expression in HCV-related HCC cases and compare these results with circSERPINA3 and microRNA-944 gene expression levels in HCV-infected patients.

METHODOLOGY: Study participants were divided into three groups: healthy controls, HCV- infected, and HCV-induced HCC patients. The gene expression levels of circSERPINA3 and microRNA-944 were evaluated using Real-Time qPCR. Then the immunoblotting procedure was applied to measure the serum levels of MDM2 and E-cadherin besides, the serum concentration levels of glypican-3 and alpha-fetoprotein were measured by sandwich ELISA.

RESULTS: The gene expression level of circSERPINA3 was significantly upregulated in both HCV-infected and HCC patients causing suppression of the antitumor effect of miR-944 and showing a lower 1-year survival rate than the participants who had low circSERPINA3 gene expression levels. Subsequently, the miR-944 downstream protein, MDM2 was remarkably upregulated, exaggerating the metastasis and oxidative stress in HCC cases. Additionally, the results confirmed the downregulation of microRNA-944 improved the progression of viral hepatitis C cases to hepatocarcinogenesis through the significantly increased serum level of the metastatic marker, E-cadherin. Although alpha-fetoprotein is a common diagnostic marker used in the diagnosis of HCC, our results showed that glypican-3 had greater sensitivity and specificity and positively correlated to the IGF-1 signaling pathway of HCC cases. Moreover, the gene expression levels of circSERPINA3 and E-cadherin in both the HCV and HCV-induced HCC were significantly positively correlated.

CONCLUSION: circSERPINA3 and miR-944 were sensitive molecular markers for early diagnosis of HCC and could be prospective treatment targets for HCV-infected patients to avoid tumor recurrence in HCC cases.

}, author = {Nora M. Aborehab and Kandeil, Mohamed A and Dina Sabry and Rabie, Radwa and Ibrahim, Ibrahim T} } @article {228906, title = {Diagnostic significance of hsa_circ_0000146 and hsa_circ_0000072 biomarkers for Diabetic Kidney Disease in patients with type 2 diabetes mellitus.}, journal = {Journal of medical biochemistry}, volume = {42}, year = {2023}, pages = {239-248}, abstract = {

BACKGROUND: Diabetic Kidney Disease (DKD) is a significant challenge in healthcare. However, there are currently no reliable biomarkers for renal impairment diagnosis, prognosis, or staging in DKD patients. CircRNAs and microRNAs have emerged as noninvasive and efficient biomarkers.

METHODS: We explored Cannabinoid receptor 1 (CNR1), C reactive protein (CRP), hsa_circ_ 0000146 and 0000072, and hsa-miR-21 and 495 as diagnostic biomarkers in DKD. The serum concentrations of CRP and CNR1 were measured using ELISA. Rt-qPCR was used to evaluate the expression levels of CNR1, circRNAs, and miRNAs in 55 controls, 55 type 2 diabetes mellitus patients, and 55 DKD patients. Their diagnostic value was determined by their ROC curve. KEGG pathway was used to predict the functional mechanism of the circRNA{\textquoteright}s target genes.

RESULTS: DKD patients exhibited a significant increase in CRP and CNR1 levels and the expression of miR-21 and 495. The expression levels of circ_0000146 and 0000072 decreased in DKD patients. ROC analysis revealed that circRNAs and miRNAs alone or CNR1 and CRP have significant diagnostic potential. The functional prediction results showed the involvement of hsa_circ_0000146 and 0000072 in various pathways that regulate DKD.

CONCLUSIONS: Therefore, the examined circRNAs and miRNAs may represent a novel noninvasive biomarker for diagnosing and staging DKD.

}, author = {Badr, Amul M and Elkholy, Omayma and Mona Said and Fahim, Sally A and El-Khatib, Mohamed and Dina Sabry and Radwa M. Gaber} } @article {244399, title = {Dyslipidemia initiates keratinocytes proliferation through upregulation of lncRNA NEAT in psoriasis patients.}, journal = {Molecular biology reports}, volume = {50}, year = {2023}, pages = {7597-7604}, abstract = {

BACKGROUND: Psoriasis is a chronic inflammatory immune-mediated and hyper proliferative skin disorder that has underlying genetic factors. Psoriasis can result from interaction of cytokines between keratinocytes and T-lymphocytes. NEAT is a lncRNA involved in immune modulation and has been previously studied in cancers. This study aims to clarify the unprecedented role of NEAT in psoriasis pathogenesis.

METHODS: The study was conducted on 50 healthy control subjects and 50 psoriasis patients. Blood samples from all participants were collected for analysis of their lipid profile. qRT-PCR was done for lncRNA NEAT, TNF-α, VEGF genes expression. The levels of ROS and caspase-3 were estimated by ELISA. ROC analysis was done to detect the diagnostic value of lncRNA NEAT gene~expression.

RESULTS: Dyslipidemia is more prevalent among psoriasis patients. A significant up regulation in lncRNA NEAT, TNF-α, VEGF genes expression (p value˂0.001) in psoriasis patients in addition to significant increase in ROS and caspase-3 levels (p value˂0.001) in compare to controls. Additionally, a positive significant correlation between TNF-α, ROS, NEAT, caspase-3 and dyslipidemia. NEAT had an area under the curve (AUC) of 0.931 (95\% CI 0.844-0.978, p \< 0.001).

CONCLUSION: Dyslipidemia is an initiating signal in psoriasis pathogenesis that creates a state of chronic inflammation and oxidative stress. This state induces keratinocytes proliferation and release of NEAT with subsequent caspase-3 activation to counteract the proliferating cells. NEAT could be considered as a good diagnostic biomarker for psoriasis.

}, author = {Abeer Mostafa and Dina Sabry and Aboraia, Nesreen and Ahmed Fawzy and Abou-Elalla, Amany A} } @article {244398, title = {The Effects of Umbilical Cord Mesenchymal Stem Cells -Derived Exosomes in Oral Squamous Cell Carcinoma (In vitro Study)}, year = {2023} } @article {244412, title = {The Effects of Umbilical Cord Mesenchymal Stem Cells -Derived Exosomes in Oral Squamous Cell Carcinoma (In vitro Study).}, journal = {Asian Pacific journal of cancer prevention : APJCP}, volume = {24}, year = {2023}, pages = {2531-2542}, abstract = {

OBJECTIVE: Mesenchymal stem cells (MSCs) derived exosomes offers several advantages as a cell-free therapeutic agents. In this study, Umbilical cord mesenchymal stem cells exosomes (UC-MSCs-exos) effects on oral squamous cell carcinoma (OSCC) cell line was evaluated.

METHODS: UC-MSCs-exos were isolated and co-cultured with OSCC cells and their impact on OSCC was explored by various tests. Comet assay and western blot for cleaved caspase-3 and immunocytochemistry for caspase-8 were used for apoptosis assessment. HO-1 and Nrf2 were used to determine antioxidant levels. Tumor necrosis factor-α and interleukin-6 were assessed as inflammatory biomarkers. HOX transcript antisense intergenic long noncoding RNA (HOTAIR) expression was also evaluated.

RESULTS: In a dose-dependent manner, UC-MSCs-exos reduced the levels of pro-inflammatory cytokines (IL-6 and TNF-α) and induced apoptosis of OSCC in vitro. Meanwhile, we found that UC-MSCs-exos downregulate HOTAIR.

CONCLUSION: UC-MSCs-exos conferred a suppressive role on OSCC in vitro, highlighting a promising therapeutic role. However, the exact potentially involved molecules and molecular mechanisms need to be investigated in further studies.

}, author = {Abdelwhab, Amira and Alaa El-Din, Yasmine and Dina Sabry and Lotfy Aggour, Reham} } @article {228910, title = {The Emerging Role of Circular RNA Homeodomain Interacting Protein Kinase 3 and Circular RNA 0046367 through Wnt/Beta-Catenin Pathway on the Pathogenesis of Nonalcoholic Steatohepatitis in Egyptian Patients.}, journal = {Reports of biochemistry \& molecular biology}, volume = {11}, year = {2023}, pages = {614-625}, abstract = {

BACKGROUND: Non-alcoholic fatty liver disease is a major problem worldwide that needs non-invasive biomarkers for early diagnosis and treatment response assessment. We aimed to assess the correlation between circRNA-HIPK3 and miRNA-29a expression and its role as miRNA-29a sponge, as well as the correlation between circRNA-0046367 and miRNA-34a expression and its role as miRNA-34a sponge and their effect on regulation of the Wnt/β catenin pathway, which may provide a new target for treatment of non-alcoholic steatohepatitis.

METHODS: the research was performed on 110 participants: group (I): fifty-five healthy donors served as controls and group (II): fifty-five patients with fatty liver pattern on abdominal ultrasound. Lipid profile and liver functions were assessed. RT-PCR was performed to assess the RNAs: circRNA-HIPK3, circRNA-0046367, miRNA-29a, miRNA-34a and mRNA gene expression. ELISA was performed to determine β-catenin protein levels.

RESULTS: miRNA-34a and circRNA-HIPK3 expression were significantly greater, while miRNA-29a and circRNA-0046367 expression were significantly less, in patients than in controls. Wnt/β-catenin regulated by miRNA-29a and miRNA-34a showed a significant decrease that leads to its abnormal effect on lipid metabolism.

CONCLUSIONS: our results imply that miRNA-29a can be investigated as a target for circRNA-HIPK3, while miRNA-34a can be investigated as a target for circRNA-0046367, and that circRNA-HIPK3 and circRNA-0046367 may have emerging roles that can affect the pathogenesis of nonalcoholic steatohepatitis through the Wnt/β-catenin pathway and thus be used as therapeutic targets for the disease.

}, author = {Abdelgwad, Marwa and Zakaria, Reem and Marzouk, Samar and Dina Sabry and Ahmed, Rasha and Badary, Hedy Ayman and Mai Samir} } @article {228908, title = {LncRNA NEAT1 and MALAT1 are involved in polycystic ovary syndrome pathogenesis by functioning as competing endogenous RNAs to control the expression of PCOS-related target genes.}, journal = {Non-coding RNA research}, volume = {8}, year = {2023}, pages = {263-271}, abstract = {

Accumulating evidence has shown an abnormal expression of several non-coding RNAs in ovarian tissues which might be closely linked with the pathogenesis of PCOS. The aim of this study was to identify competing endogenous (ce) RNA network: long non-coding RNA (lncRNA), microRNA (miRNA) and their target genes: androgen receptor (AR), follistatin (FST) and insulin receptor substrate-2 (IRS-2), which are relevant to PCOS, to underline the molecular pathogenesis of PCOS and assist in early diagnosis and treatment. Bioinformatic analysis was performed to retrieve a ceRNA network: [lncRNA (NEAT1 and MALAT1) - miRNA (miR-30a-5p and miR-30d-5p) - mRNA (AR, FST and IRS-2)] linked to PCOS. Expression of the selected RNAs was examined by qPCR in peripheral blood leukocytes obtained from 73 PCOS patients (41 obese and 32 non-obese) and 31 healthy controls. PCOS patients showed significantly higher expression levels of NEAT1, miR-30a-5p, AR, FST and IRS-2, with significantly lower expression levels of MALAT1 and miR-30d-5p relative to controls especially in obese versus non-obese patients. Receiver operating characteristic (ROC) curve analysis indicated that most of the selected RNAs could serve as potential early diagnostic markers for PCOS with the highest efficiency obtained upon combining NEAT1 and miR-30d-5p or MALAT1 and miR-30a-5p with either of PCOS target genes. Moreover, all addressed RNAs had been proved as potential predictors of PCOS. The obtained data of ceRNA network raised the possibility that NEAT1 overexpression may increase the expression levels of AR, FST and IRS-2 by sponging miR-30d-5p, while low expression of MALAT1 may allow higher expression of the above genes via increasing miR-30a-5p, suggesting their involvement in PCOS pathogenesis and promising role for future diagnosis and targeted therapy.

}, author = {ElMonier, Asmaa A and Noha A El-Boghdady and Fahim, Sally A and Dina Sabry and Khaled A. Elsetohy and Amira A. Shaheen} } @article {228911, title = {Potential role of human umbilical cord stem cells-derived exosomes as novel molecular inhibitors of hepatocellular carcinoma growth.}, journal = {Apoptosis : an international journal on programmed cell death}, year = {2023}, abstract = {

Hepatocellular carcinoma (HCC) is one of the most critical cancers; thus, novel therapeutical regimens are of great need. In this study, we investigated the effects of umbilical cord mesenchymal stem cells (UC-MSCs) derived exosomes on HepG2 cell line, and the underlying mechanism to control HCC proliferation, to identify the potential clinical role of exosomes as a novel molecular therapeutic target. Proliferation, apoptosis, and angiogenesis effects were assessed together with the cell viability evaluation by MTT assay in HepG2 cells at 24/48~h. with or without UC-MSCs-derived exosomes. Gene expressions of TNF-α, caspase-3, VEGF, stromal cell-derived factor-1 (SDF-1), and CX chemokine receptor-4 (CXCR-4) were measured by quantitative real-time PCR technique. Expression of sirtuin-1 (SIRT-1) protein was detected by western blot. Treatment of HepG2 cells with UC-MSCs-derived exosomes for 24 and 48~h. demonstrated a significant reduction of cells survival compared to the control group (p \< 0.05). The SIRT-1 protein, and VEGF, SDF-1, CXCR-4 expression levels were significantly lower, TNF-α and caspase-3 expression levels were significantly higher in exosomal-treated HepG2 cells for 24 and 48~h. than those in the control group. Moreover, our findings documented that the anti-proliferative, apoptotic, and anti-angiogenic effects were achieved in a time-dependent manner in which more effects were determined after 48~h supplementation compared to 24~h (p \< 0.05). UC-MSCs-derived exosomes exert anticarcinogenic molecular effects on HepG2 cells through the involvement of SIRT-1, SDF-1, and CXCR-4. Hence, exosomes would be a potential novel therapy regimen against HCC. Large-scale studies are recommended to verify this conclusion.

}, author = {ElBadre, Hala M and El-Deek, Sahar E M and Ramadan, Haidi Karam-Allah and Elbadr, Mohamed M and Dina Sabry and Ahmed, Noran M and Ahmed, Amr M and El-Mahdy, Reham I} } @article {244405, title = {Protective efficacy of Toxoplasma gondii infected cells-derived exosomes against chronic murine toxoplasmosis.}, journal = {Acta tropica}, volume = {248}, year = {2023}, pages = {107041}, abstract = {

Exosomes were isolated from T. gondii infected human hepatoblastoma cells using the exosome isolation kit and characterized by electron microscopy and Western blotting. Exosomes adsorbed to alum adjuvant were evaluated as a potential immunizing agent against murine chronic toxoplasmosis compared to excretory secretory antigens (ESA)-alum. Mice were immunized at days 1, 15 and 29. The levels of IgG, IFN-γ, IL-4 and IL-10, CD4 and CD8 T cells were determined using sandwich enzyme-linked immunosorbent assay (sandwich ELISA) at days 14, 28 and 56 of the experiment. Then mice were infected orally with 10 cysts of T. gondii. The protective efficacy of the antigens were evaluated by counting the brain cysts and measuring the aforementioned humoral and cellular parameters 60 days post infection. The results showed that alum increased the protective efficacy of the exosomes. Immunization with exosome-alum induced both humoral and mixed Th1/Th2 cellular immune responses. Exosome-alum gave higher levels of the humoral and cellular parameters, compared to ESA-alum. After challenge infection, exosome-alum significantly reduced the brain cyst burden by 75~\% while ESA-alum gave 42~\% reduction and evoked higher humoral and cellular immune responses. Therefore, the possibility of using T. gondii infected cells-derived exosome-alum as a vaccine is a new perspective in toxoplasmosis.

}, author = {Gihan M. Tawfeek and Abou-El-Naga, Iman F and Hassan, Eman Mohamed Elsayed and Dina Sabry and Meselhey, Rasha Abdallah and Younis, Salwa Sami} } @article {228907, title = {Screening for maternal cytomegalovirus infection during pregnancy and pregnancy outcome in patients with liver disease: an observational study.}, journal = {BMC infectious diseases}, volume = {23}, year = {2023}, pages = {210}, abstract = {

BACKGROUND: Cytomegalovirus (CMV) infection among pregnant females could induce CMV hepatitis with possible changes in liver stiffness measurement (LSM) which could be reversibly increased during normal pregnancies, particularly in the third trimester. This study aimed to detect the prevalence of CMV infection among pregnant females with and without chronic liver disease and to evaluate the effects of CMV infection on LSM and pregnancy outcomes in comparison to non-CMV-infected pregnant females.

METHODS: This is an observational prospective study that included 201 pregnant ladies presented to the liver disease with pregnancy clinic, Cairo University from March 2018 to April 2019. We assessed the laboratory results, abdominal ultrasonography, LSM using ARFI elastography, and pregnancy outcomes.

RESULTS: Two hundred and one pregnant ladies were divided into ; group 1: pregnant ladies with normal pregnancy (n = 128), group 2: pregnant ladies with chronic liver diseases not related to pregnancy (n = 35), and group 3: pregnant ladies with pregnancy-related liver diseases (n = 38). Positive CMV serology (either/or, +ve CMV-IgM, IgG) was detected in 106/201 patients (52.74\%), and fifteen of them had an active infection (IgG +, IgM+, PCR+). Pregnant females with chronic liver diseases not related to pregnancy had significantly higher serum levels of CMV IgM, IgG, and PCR. Moreover, LSM had a significant correlation with CMV IgG and CM_PCR in normal pregnant ladies. Maternal mortality occurred only in pregnant females with chronic liver diseases in 5.7\% (2/35).

CONCLUSION: Maternal CMV infection carries a significant risk to pregnant females with chronic liver disease. Routine CMV screening for women planning to be pregnant, especially those with chronic liver disease could help to avoid bad maternal and fetal outcomes.

}, author = {Rasha El Etreby and Rasha Abd ELaziz and Hend Ibrahim Shousha and Hammam, Zeinab and Ayman Hany and Dina Sabry and Basma Elawady and Zayed, Naglaa and Ayman Yosry and Shereen Abdel Alem} } @article {228903, title = {Anti-arthritic activity of the flavonoids fraction of ivy leaves (Hedera helix L.) standardized extract in adjuvant induced arthritis model in rats in relation to its metabolite profile using LC/MS.}, journal = {Biomedicine \& pharmacotherapy = Biomedecine \& pharmacotherapie}, volume = {145}, year = {2022}, pages = {112456}, abstract = {

Ivy leaves (Hedera helix) is a traditional plant used for common cold, cough, and bronchial disorders and can be used for rheumatoid arthritis (RA) as an attempt in alternative medicine. RA is a chronic autoimmune disease characterized by its increasing frequency and adverse consequences. There is an urgent need for a long-term therapy that has favorable biological effects and is less expensive than the already authorized synthetic medicines. This study aimed to determine the anti-arthritic potentials of Hedera helix with determination of the bioactive fraction and discovery of its second-generation metabolites by means of LC/MS. The total ivy ethanolic extract (TIE-E), saponins fraction (Sap-F) and flavonoids fraction (Flav-F) were investigated for their in-vitro anti-arthritic effects and in-vivo by Adjuvant-induced arthritis (AIA) using Complete Freund{\textquoteright}s Adjuvant (0.1~mL, CFA) intradermal relative to the usual dose of ibuprofen (5~mg/kg). We examined the physical alterations, rheumatoid biomarkers, cytokines that cause and inhibit inflammation, markers of oxidative stress, hyaluronidase and β-glucuronidase enzyme activity. Each paw{\textquoteright}s histopathology was also evaluated. The chemical profiles of TIE-E were studied using LC/MS in both positive and negative ionization modes. TIE-E (200~mg/kg) and Flav-F (100~mg/kg) significantly (P~\< 0.05) lowered the edema of the paws, serum immunological indicators, inflammatory cytokines, degenerative enzymes, and indicators of reactive oxygen species with increasing in the anti-inflammatory cytokines. Our findings suggest that extracts of ivy leaves might be used effectively to treat rheumatoid arthritis, where its flavonoid content is responsible for that, and it is able to repress biochemical, oxidative, and pathological changes associated with (AIA) Adjuvant-induced arthritis.

}, author = {Aya A. Shokry and Riham A. El-Shiekh and Gehan Kamel and Alaa F Bakr and Dina Sabry and Amer Ramadan} } @article {228900, title = {The anti-tumour effect of induced pluripotent stem cells against submandibular gland carcinoma in rats is achieved via modulation of the apoptotic response and the expression of Sirt-1, TGF-β, and MALAT-1 in cancer cells.}, journal = {Molecular and cellular biochemistry}, volume = {477}, year = {2022}, pages = {53-65}, abstract = {

The era of induced pluripotent stem cells (iPSCs) was used as novel biotechnology to replace embryonic stem cells bypassing the ethical concerns and problems of stem cell transplant rejection. The anti-tumour potential of iPSCs against many tumours including salivary cancer was proven in previous studies. The current study aimed to investigate the contribution of the Bax, Sirt-1, TGF-β, and MALAT genes and/or their protein expression to the pathogenesis of submandibular carcinogenesis before and after iPSCs treatment. Thirty Wistar albino rats were equally assigned into three groups: group I (control), group II (Squamous cell carcinoma (SCC)): submandibular glands were injected SCC cells, and group III (SCC/iPSCs): SCC rats were treated by 5 {\texttimes} 10 iPSCs. Submandibular gland sections were subjected to histological and immunohistochemical analyses to detect mucopolysaccharides, Bax, and TGF-β expression as well as PCR quantification for TGF-β, Sirt-1, and lncRNA MALAT-1 gene expressions. Western blotting was also used to detect Sirt-1 and TGF-β protein expressions. SCC group revealed infiltration by sheets of malignant squamous cells with or without keratin pearls and inflammatory cells, in addition to upregulation of TGF-β, Sirt-1, MALAT-1, and Bax, whereas SCC/iPSCs group showed an improved submandibular histoarchitecture with the maintenance of the secretory function. Bax and TGF-β immunoexpression were significantly reduced. The upregulated TGF-β, Sirt-1, and MALAT-1 genes were significantly decreased. iPSCs protected against the experimentally induced submandibular gland carcinoma that might be achieved via their regenerative potential and their regulatory modulation of Sirt-1, TGF-β, and MALAT-1 gene/protein expressions and of the apoptotic response in cancer cells.

}, author = {Eman Mohamed Faruk and Dina Sabry and Morsi, Ahmed A and Alaa El-Din, Yasmine and Taha, Neama M and Engy Medhat} } @article {228892, title = {Assessing the bone-healing potential of bone marrow mesenchymal stem cells in jawbone osteoporosis in albino rats.}, journal = {Dental and medical problems}, volume = {59}, year = {2022}, pages = {75-83}, abstract = {

BACKGROUND: Osteoporosis is one of the most common yet difficult to treat diseases. It affects millions of people and costs the health care systems billions worldwide. All of the available kinds of pharmacological treatment have multiple side effects, which is why a need for safer treatment options has emerged.

OBJECTIVES: This study aimed to assess the bone-healing potential of bone marrow mesenchymal stem cells (BM-MSCs) in jawbone osteoporosis in Wistar albino rats.

MATERIAL AND METHODS: Osteoporosis was induced with a daily intraperitoneal injection of 200 μg/100 g dexamethasone for 1 month. The rats were then randomly distributed into 2 groups: the osteoporotic group (left untreated); and the BM-MSCs group (received an intravenous injection of 50 million cultured BM-MSCs). Half of the rats from each group were sacrificed 2 weeks and the other half 6 weeks after the introduction of treatment. Bone regeneration was assessed by means of dual-energy X-ray absorptiometry (DEXA), real-time polymerase chain reaction (RT-PCR), as well as the histopathological and histomorphometric analyses.

RESULTS: As for the 1st sacrifice time, there were no significant differences between the osteoporotic and BM-MSCs groups with regard to all parameters except for bone mineral density (BMD), which was significantly higher in the BM-MSCs group. Regarding the 2nd sacrifice time, the DEXA analysis showed a significant increase in BMD in the BM-MSCs group (p \< 0.001). The RT-PCR analysis showed a significant decrease in RANKL gene expression (p \< 0.001) and a significant increase in OPG gene expression (p \< 0.001) in the BM-MSCs group. In addition, the histopathological examination of the BM-MSCs group showed pronounced healing progress in the jawbone microarchitecture. The histomorphometric analysis also revealed that the bone area percentage significantly increased in the BM-MSCs group (p \< 0.001).

CONCLUSIONS: This study proved that BM-MSCs could be effective in the treatment of osteoporosis.

}, author = {Tasneem Soliman and Zoba Ali and Mohamed Zayed and Dina Sabry and Nermeen AbuBakr} } @article {228899, title = {Assessment of ultra-structure, viability and function of lipopolysaccharides-stimulated human dermal fibroblasts treated with chrysin and exosomes ().}, journal = {The Saudi dental journal}, volume = {34}, year = {2022}, pages = {346-354}, abstract = {

BACKGROUND: Lipopolysaccharides (LPS) stimulate production of inflammatory cytokines. Chrysin is flavonoid beneficial for treatment of inflammatory conditions. Bone marrow mesenchymal stem cell (BM-MSC) exosomes have regenerative ability in different tissues.

OBJECTIVE: To assess potential role of chrysin and BM-MSC exosomes on ultra-structure, viability and function of human~dermal~fibroblasts-adult (HDFa) stimulated by LPS.

METHODS: HDFa cells were divided into: Cells received no treatment. Cells were stimulated with LPS. LPS stimulated cells were treated with chrysin. LPS stimulated cells were treated with exosomes.

RESULTS: After 48~h, ultrastructural examination of HDFa cells in Group I revealed intact plasma membrane and numerous cytoplasmic organelles. Group II displayed destructed plasma membrane and apoptotic bodies. Group III showed intact plasma membrane with loss of its integrity at some areas. Group IV demonstrated intact plasma membrane that showed fusion with exosomes at some areas. Statistical analysis of MTT represented highest mean value of cell viability\% in Group IV followed by Groups III, I and II respectively. Statistical analysis of enzyme-linked immunosorbent assay (ELISA) showed the highest mean value of interleukin-1β (IL-1β) was in Group II followed by Groups III, IV and I, while highest mean values of interleukin-10 (IL-10), nuclear factor-erythroid 2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) proteins were in Group I, followed by Groups IV, III and II respectively.

CONCLUSIONS: LPS have harmful consequences on ultra-structure, viability and function of HDFa cells. BM-MSC exosomes have better regenerative action on inflamed fibroblasts in comparison to chrysin.

}, author = {Hassan, Rabab and Dina Sabry and Amany A. Rabea} } @article {228894, title = {Comparative study on effect of mesenchymal stem cells and endothelial progenitor cells on treatment of experimental CCL4-induced liver fibrosis.}, journal = {Archives of physiology and biochemistry}, volume = {128}, year = {2022}, pages = {1071-1080}, abstract = {

BACKGROUND: We speculated impacts of BM-MSCs and UC-EPCs on reversal of hepatic injury induced by carbon tetrachloride (CCl4). Fifty adult rats were divided into five groups: control group, CCl4A group, CCl4B group, CCl4/BM-MSCs group and CCl4/UC-EPCs group. Blood samples were driven to measure concentration of albumin and ALT. Quantitative expression of HGF, TGF-β, MMP-2, and VEGF were assessed by PCR. Histological and immunohistochemistry examination of the liver tissue were performed.

RESULTS: There was elevating albumin ( \< .05) and reducing ALT ( \< .05) concentrations in groups treated with BM-MSCs and UC-EPCs compared to untreated CCL4A\&B groups. UC-EPCs treated group have significantly higher MMP-2 and VEGF ( \< .01) genes expression than BM-MSCs treated group. Furthermore, UC-EPCs were more valuable than BMMSCs in increasing gene expression of HGF ( \< .05) and immunohistochemistry of α-SMA and Ki-67 ( \< .01). BM-MSCs have significantly lower TGF-β ( \< .00) compared to UC-EPCs.

CONCLUSION: This study highlighted on liver regeneration role of both UC-EPCs and BM-MSCs in liver fibrosis.

}, author = {Abdelgwad, Marwa and Manal Ewaiss and Dina Sabry and Warda A. Khalifa and Altaib, Zeinab M and Maha alhelf} } @article {228902, title = {Cromolyn chitosan nanoparticles reverse the DNA methylation of RASSF1A and p16 genes and mitigate DNMT1 and METTL3 expression in breast cancer cell line and tumor xenograft model in mice.}, journal = {Chemico-biological interactions}, volume = {365}, year = {2022}, pages = {110094}, abstract = {

BACKGROUND: Developing epigenetic drugs for breast cancer (BC) remains a novel therapeutic approach. Cromolyn is a mast cell stabilizer emerging as an anticancer drug; its encapsulation in chitosan nanoparticles (CSNPs) improves its effect and bioavailability. However, its effect on DNA and RNA methylation machineries has not been previously tackled.

METHODS: The possible anticancer effect of cromolyn CSNPs and its potential as an epigenetic drug was investigated in vitro using MCF-7 human BC cell line and in vivo using Ehrlich ascites carcinoma-xenograft model in mice symbolizing murine mammary adenocarcinoma. Mice were injected with a single dose of Ehrlich ascites carcinoma cells subcutaneously for the induction of tumor mass, and then randomized into three groups: control, cromolyn CSNPs (equivalent to 5~mg cromolyn/kg, i.p.) and plain CSNPs twice/week for 2 weeks.

RESULTS: Cromolyn CSNPs showed prominent anticancer effect in MCF-7~cells by reducing the cell viability percent and enhancing DNA damage in the comet assay demonstrating its apoptotic actions. Mechanistically, cromolyn CSNPs influenced potential epigenetic processes through mitigating DNA methyltransferase 1 (DNMT1) expression, reversing the hypermethylation pattern of the tumor suppressor RASSF1A and p16 genes and attenuating the expression of the RNA N-methyladenosine writer, methyltransferase-like 3 (METTL3). Cromolyn CSNPs diminished ERK1/2 phosphorylation, a possible arm influencing DNMT1 expression. In vivo, cromolyn CSNPs lessened the tumor volume and halted DNMT1 and METTL3 expression in Ehrlich carcinoma mice.

CONCLUSIONS: Cromolyn CSNPs have the premise as an epigenetic drug through inhibiting ERK1/2 phosphorylation/DNMT1/DNA methylation and possibly impacting the RNA methylation machinery via mitigating METTL3 expression.

}, author = {Tarek K. Motawi and Shohda A. EL-Maraghy and Dina Sabry and Nady, Omina M and Mahmoud A Senousy} } @article {228904, title = {Differential Sensitivity of Head and Neck Squamous Cell Carcinoma to Cisplatin, Silver Nanoparticles, and Photodynamic Therapy.}, journal = {Reports of biochemistry \& molecular biology}, volume = {11}, year = {2022}, pages = {224-237}, abstract = {

BACKGROUND: The clinical effect of photodynamic therapy (PDT) may be correlated with the degree of dysplasia of cancer tissues. The aim of this study was to compare the effects of cisplatin, silver nanoparticles (AgNps), and photodynamic therapy (PDT) using methylene blue (MB) photosensitizer on Head and Neck squamous cell carcinoma - cell line (HNSCC), Hep-2, through genes expression.

METHODS: Hep-2 cells were divided into four groups: group I as control and without any treatment, group II and III were treated by cisplatin and AgNps, respectively, and group IV were incubated with MB for four minutes followed by PDT using laser irradiation at 650 nm for 8 minutes. The resulting toxicity was assessed in cell lines using MTT cytotoxicity assay. Further, apoptosis and the response to treatment was examined via RT-qPCR.

RESULTS: MB-PDT inhibited the proliferation of Hep-2 cells. Following PDT, compared with AgNps cells and via MTT assay, a highly significant decrease was observed in cell proliferation in Cancer cells treated with AgNps and MB- PDT groups compared to cancer group cells and cancer cells treated with Cisplatin (p value\< 0.001). Mechanistically, both the mRNA and protein expression levels of , , , , , , and were found to be down regulated in Hep-2 cell line after MB-PDT.

DISCUSSION: MB-PDT effectively killed Hep-2 cells in vitro, however, under the same conditions, the susceptibilities of the cell line to cisplatin, AgNps, and MB-PDT were different. Further studies are necessary to confirm whether this difference is present in clinical oral cancer lesions.

}, author = {Abdelgwad, Marwa and Dina Sabry and Latifa Mohamed Abdelgawad and Mohamed Elroby Ali, Doaa} } @article {228895, title = {Effect of bone marrow mesenchymal stem cells-derived exosomes on diabetes-induced retinal injury: Implication of Wnt/ b-catenin signaling pathway.}, journal = {Biomedicine \& pharmacotherapy = Biomedecine \& pharmacotherapie}, volume = {154}, year = {2022}, pages = {113554}, abstract = {

BACKGROUND: Diabetic retinopathy (DR) is a serious microvascular complication of diabetes mellitus. Mesenchymal stem cells are currently studied as therapeutic strategy for management of DR. Exosomes, considered as a promising cell-free therapy option, display biological functions similar to those of their parent cells. In retinal development, Wnt/b-catenin signaling provides key cues for functional progression. The present study aimed to evaluate the potential efficacy of bone marrow-derived mesenchymal stem cell-derived exosomes (BM-MSCs-Ex) in diabetes-induced retinal injury via modulation of the Wnt/ b-catenin signaling pathway.

METHODS: Eighty-one rats were allocated into 6 groups (control, DR, DR + DKK1, DR + exosomes, DR + Wnt3a and DR + exosomes+Wnt3a). Evaluation of each group was via histopathological examination, assessment of gene and/or protein expression concerned with oxidative stress (SOD1, SOD2, Nox2, Nox4, iNOS), inflammation (TNF-α, ICAM-1, NF-κB) and angiogenesis (VEGF, VE-cadherin).

RESULTS: Results demonstrated that exosomes blocked the wnt/b-catenin pathway in diabetic retina concomitant with significant reduction of features of DR as shown by downregulation of retinal oxidants, upregulation of antioxidant enzymes, suppression of retinal inflammatory and angiogenic markers. These results were further confirmed by histopathological results, fundus examination and optical coherence tomography. Additionally, exosomes ameliorative effects abrogated wnt3a-triggered retinal injury in DR.

CONCLUSION: Collectively, these data demonstrated that exosomes ameliorated diabetes-induced retinal injury via suppressing Wnt/ b-catenin signaling with subsequent reduction of oxidative stress, inflammation and angiogenesis.

}, author = {Ebrahim, Nesrine and El-Halim, Heba Elsayed Abd and Helal, Omayma Kamel and El-Azab, Nahla El-Eraky and Badr, Omnia A M and Hassouna, Amira and Saihati, Hajir A Al and Aborayah, Nashwa Hassan and Emam, Hanan Tawfeek and El-Wakeel, Hend S and Aljasir, Mohammad and Mohamed El-Sherbiny and Sarg, Naglaa A S and Shaker, Gehan Ahmed and Ola Mostafa and Dina Sabry and Fouly, Marwa Abdel Kader and Forsyth, Nicholas Robert and Elsherbiny, Nehal M and Salim, Rabab F} } @article {228887, title = {Effect of Diacerein on HOTAIR/IL-6/STAT3, Wnt/β-Catenin and TLR-4/NF-κB/TNF-α axes in colon carcinogenesis.}, journal = {Environmental toxicology and pharmacology}, volume = {95}, year = {2022}, pages = {103943}, abstract = {

Colorectal cancer (CRC) is a common malignancy with high mortality and poor prognosis. Diacerein (DIA) is an anti-inflammatory used for treatment of osteoarthritis. We delineated some underlying molecular mechanisms of DIA{\textquoteright}s anti-carcinogenic effect in CRC using in vivo and in vitro models. Human Caco-2 cells were treated with DIA followed by MTT and Annexin V assays and CRC was experimentally induced using 1,2-dimethylhydrazine. DIA (50~mg/kg/day, orally) was administrated for 8 weeks. The MTT assay confirmed cytotoxic effect of DIA in vitro and Annexin V confirmed its apoptotic effect. DIA resulted in regression of tumour lesions with reduced colonic TLR4, NF-κB and TNF-α protein levels and down-regulated VEGF expression, confirming anti-angiogenic impact. DIA triggered caspase-3 expression and regulated Wnt/β-Catenin pathway, by apparently interrupting the IL-6/STAT3/ lncRNA HOTAIR axis. In conclusion, DIA disrupted IL-6/STAT3/ lncRNA HOTAIR axis which could offer an effective therapeutic strategy for the management of CRC.

}, author = {Eisa, Nada H and Eman Said and Khodir, Ahmed E and Dina Sabry and Ebrahim, Hasnaa Ali and Elsherbini, Dalia Mahmoud Abdelmonem and Altemani, Reem and Alnasser, Dania Mohammed and Elsherbiny, Nehal M and Mohamed El-Sherbiny} } @article {228888, title = {FOXD1-mTOR Signaling Pathway on Oral Squamous Cell Carcinoma and Its Inhibition by Rosemary Extract (Invitro-Study).}, journal = {Asian Pacific journal of cancer prevention : APJCP}, volume = {23}, year = {2022}, pages = {3071-3081}, abstract = {

BACKGROUND: FOXD1 expression in oral squamous cell carcinoma remains uncovered. The aim was to detect the anticancer effect of Rosemary Extract RE through the evaluation of FOXD1 gene expression in (OSCC) by quantitative PCR.

METHODS: OSCC cell line was served as a control group. Moreover, the OSCC cell line (SCC-15) was treated with RE (OSCC/ RE group) at 24, 48, and 72 hs time intervals. We assessed the antioxidant activity of RE by evaluation of lipid peroxidation (MDA) and superoxide dismutase (SOD) levels. The cytotoxic effects of RE were examined by MTT assay. mTOR and LC3 I/II autophagy protein markers were assessed by western blot. Apoptosis activity was assessed.

RESULTS: The study results were statistically assessed. Intergroup comparisons were analyzed, whereas intragroup comparisons were conducted utilizing one-way repeated measures ANOVA, followed by multiple pairwise paired t-tests with Bonferroni correction revealed a significant increase of FOXD1 gene expression in the control OSCC group in comparison to the OSCC/RE group (p-value \<0.001). A significant decrease of mTOR/LC3I/II proteins expression in the OSCC/RE group compared to the control OSCC group (p-value \<0.001).

CONCLUSION: FOXD1 can be considred a diagnostic biomarker for OSCC. RE inhibits autophagy of oral human cancer cells via mTOR/LC3I/II-dependent pathways and decrease caspase -3 apoptotic level.

}, author = {Alaa El-Din, Yasmine and Dina Sabry and Sahar H. Ahmed and Abbas Mohamed} } @article {228897, title = {Frequency of Genotypes and Allelic Polymorphisms of Vitamin D Receptor in Egyptian Psoriatic Patients and Their Association With Disease Severity, Immune Modulation of IL-22 Levels and The Response to Topical Calcipotriol Treatment: A Case Control Study.}, journal = {Indian journal of dermatology}, volume = {67}, year = {2022}, pages = {37-44}, abstract = {

OBJECTIVE: This study was performed to determine the genotype and allelic frequencies (polymorphisms) of the four genes of vitamin D receptor (VDR) among Egyptian psoriatic patients and healthy controls to explore their association with disease severity (PASI) score and immune modulation of IL-22 cytokine and to predict the response to topical calcipotriol treatment.

PATIENTS AND METHODS: The frequencies of the four VDR gene polymorphisms (FokI, ApaI, TaqI, and BsmI) in blood samples of 51 adult Egyptian patients with psoriasis vulgaris and 50 healthy controls were evaluated using restriction fragment length polymorphism (RFLP)-PCR. Serum levels of IL-22 were measured by ELISA.

RESULTS: The most frequent genotype (wild) in the studied patients was Apa1; AA (88.2\%) followed by Fok1; FF (47.1\%) and Taq1; TT (47\%), while Bsm1; BB genotype was (27.7\%). The most frequent allele polymorphisms either in one allele (Bb) or both alleles (bb) in psoriatic patients were 72.5\%, followed by Ff, ff (52.9\%) and Tt, tt (52.9\%). The less frequent allelic polymorphism was Aa, aa (27.7\%). Insignificant differences in the frequency of genotype (wild) and allelic polymorphisms were detected between patients and controls ( \> 0.05). A significantly higher serum concentration of IL-22 (ng/mL) was detected in patients than controls ( = 0.001). Further, 66.6\% of patients displayed a clinical response, while 33.4\% were non-responders. A significantly higher expression of TaqI polymorphism was detected in (100\%) of non-responders ( \< 0.001), which was also correlated with disease severity (r = 0.515, \< 0.01).

CONCLUSION: These results suggest that the VDR TaqI polymorphism is the only gene correlated to psoriasis susceptibility in the Egyptian population, and affects the response to topical calcipotriol treatment but does not affect IL-22 immune modulation.

}, author = {A Anani, Haneya A and Warda A. Khalifa and Althoqapy, Azza Ali and Maklad, Soheir S and Hoda Kamal and Dina Sabry and Mai Samir} } @article {228905, title = {Impact of different processing methods on the phenolics and neuroprotective activity of Duch. extracts in a D-galactose and aluminum chloride-induced rat model of aging.}, journal = {Food \& function}, volume = {13}, year = {2022}, pages = {7794-7812}, abstract = {

Age-related diseases, including dementia, are a major health concern affecting daily human life. Strawberry ( Duch.) is the most eaten fruit worldwide due to its exceptional aroma and flavor. However, it{\textquoteright}s rapid softening and decay limit its shelf-life. Freezing and boiling represent the well-known conservation methods to extend its shelf-life. Therefore, we aimed to discover the phytochemical content differences of fresh and processed strawberries associated with investigating and comparing their neuroprotective effects in a rat model of aging. Female Wistar rats were orally pretreated with fresh, boiled, and frozen methanolic extracts (250 mg kg) for 2 weeks, and then these extracts were concomitantly exposed to D-galactose [65 mg kg, subcutaneously (S/C)] and AlCl (200 mg kg, orally) for 6 weeks to develop aging-like symptoms. The results of UPLC/ESI-MS phytochemical profiling revealed 36 secondary metabolites, including phenolics, flavonoids, and their glycoside derivatives. Compared with boiled and frozen extracts, the fresh extract ameliorated the behavioral deficits including anxiety and cognitive dysfunction, upregulated brain HO-1 and Nrf2 levels, and markedly reduced caspase-3 and PPAR-γ levels. Moreover, LDH and miRNA-9, 124 and 132 protein expressions were reduced. The histological architecture of the brain hippocampus was restored and glial fibrillary acidic protein (GFAP) immunoexpression was downregulated. In conclusion, the fresh extract has neuroprotective activity that could have a promising role in ameliorating age-related neurodegeneration.

}, author = {Marwa I. Ezzat and Marwa Y. Issa and Sallam, Ibrahim E and Dalia Zaafar and Heba M. A. Khalil and Mohamed R Mousa and Dina Sabry and Aya Y. Gawish and Ahmed H. Elghandour and Engy Mohsen} } @article {228890, title = {Impact of FOXP1 rs2687201 genetic variant on the susceptibility to HCV-related hepatocellular carcinoma in Egyptians.}, journal = {Journal of biochemical and molecular toxicology}, volume = {36}, year = {2022}, pages = {e22965}, abstract = {

Hepatocellular carcinoma (HCC) constitutes a challenging health problem in Egypt due to the high incidence of hepatitis C virus (HCV) infection. Improved understanding of genetic mechanisms underlying the individual predisposition to HCC will lead to enhancements in the early diagnosis, treatment, and prevention of this disease. Transcription factor forkhead box P1 (FOXP1) is involved in the cellular processes of proliferation, differentiation, metabolism, and longevity. In addition, it has been implicated in hepatic tumorigenesis. The present study explored the association of C/A single-nucleotide polymorphism in the FOXP1 gene (rs2687201) with HCC susceptibility in HCV Egyptian patients. The study included 108 patients with HCV-dependant HCC, 86 HCV patients, and 80- age and gender-matched healthy controls. rs2687201 genotyping was performed by allelic discrimination method using TaqMan real-time PCR assays while FOXP1 gene expression and protein level were determined using qRT-PCR and enzyme-linked immunoassay, respectively. Our results revealed a significant association between FOXP1 rs2687201 and HCC risk where (A) allele was significantly more frequent in patients with HCC compared to controls (odds ratio [OR]: 1.88, 95\% confidence interval [CI]: 1.17-3.04, p = 0.01) and to HCV patients (OR: 1.85, 95\% CI: 1.62-2.94, p = 0.012). Furthermore, FOXP1 gene and protein expression levels were remarkably higher in (CA + AA) than in CC genotype carriers in a dominant model. The (CA + AA) genotype displayed a significantly shorter overall survival than the CC genotype in HCC patients. In conclusion, FOXP1 gene polymorphism rs2687201 is significantly associated with HCC, but not with HCV infection, in Egyptian patients.

}, author = {Motawi, Tarek Mohamed Kamal and Dina Sabry and Nagwa Ibrahim Shehata and William, Mira Magdy and Atef Tadros Fahim} } @article {228901, title = {In vitro evaluation of the effect of the electronic cigarette aerosol, Cannabis smoke, and conventional cigarette smoke on the properties of gingival fibroblasts/gingival mesenchymal stem cells.}, journal = {Journal of periodontal research}, volume = {57}, year = {2022}, pages = {104-114}, abstract = {

OBJECTIVE: The current study aimed to evaluate the effect of electronic cigarette (EC) aerosol, Cannabis, and conventional cigarettes smoke on gingival fibroblast/gingival mesenchymal stem cells{\textquoteright} (GF/G-MSCs) of never smokers.

MATERIAL AND METHODS: Human GF/G-MSCs (n~=~32) were isolated and characterized using light microscopy, flow cytometry, and multilineage differentiation ability. Following the application of aerosol/smoke extracts, GF/G-MSCs were evaluated for cellular proliferation; colony-forming units (CFU-F) ability; cellular viability (using the MTT assay); mitochondrial depolarization using JC-1 dye; and genes{\textquoteright} expression of ATM, p21, Oct4, and Nanog.

RESULTS: Colony-forming units and viability (OD 450~nm) were significantly reduced upon exposure to Cannabis (mean~{\textpm}~SD; 5.5~{\textpm}~1.5; p~\<~.00001, 0.47~{\textpm}~0.21; p~\<~.05) and cigarettes smoke (2.3~{\textpm}~1.2 p~\<~.00001, 0.59~{\textpm}~0.13, p~\<~.05), while EC aerosol showed no significant reduction (10.8~{\textpm}~2.5; p~=~.05, 1.27~{\textpm}~0.47; p~\>~.05) compared to the control group (14.3~{\textpm}~3, 1.33~{\textpm}~0.12). Significantly upregulated expression of ATM, Oct4, and Nanog (gene copies/GADPH) was noticed with Cannabis (1.5~{\textpm}~0.42, 0.82~{\textpm}~0.44, and 1.54~{\textpm}~0.52, respectively) and cigarettes smoke (1.52~{\textpm}~0.75, 0.7~{\textpm}~0.14, and 1.48~{\textpm}~0.79, respectively; p~\<~.05), whereas EC aerosol caused no statistically significant upregulation of these genes compared to the control group (0.63~{\textpm}~0.1, 0.31~{\textpm}~0.12, and 0.64~{\textpm}~0.46, respectively; p~\>~.05). The p21~gene was not significantly downregulated in EC aerosol (1.22~{\textpm}~0.46), Cannabis (0.71~{\textpm}~0.24), and cigarettes smokes (0.83~{\textpm}~0.54) compared to the control group (p~=~.053, analysis of variance).

CONCLUSION: Cannabis and cigarettes smoke induce DNA damage and cellular dedifferentiation and negatively affect the cellular proliferation and viability of GF/G-MSCs of never smokers, whereas EC aerosol showed a significantly lower impact on these properties.

}, author = {El-Mouelhy, Abir Tarek Mansour and Nasry, Sherine Adel and El-Dahab, Omnia Abou and Dina Sabry and Fawzy El-Sayed, Karim} } @article {228886, title = {Nano Methotrexate versus Methotrexate in Targeting Rheumatoid Arthritis.}, journal = {Pharmaceuticals (Basel, Switzerland)}, volume = {16}, year = {2022}, abstract = {

Nanomedicine has emerged as an important approach for targeting RA medication. Rheumatoid arthritis (RA) is a widespread autoimmune disorder marked by multiple inflamed joints. Gold nanoparticles (GNPs) have been demonstrated as efficacious nanocarriers due to their unique characteristics and the relative simplicity of their synthesis in varied sizes; moreover, they have the capability to alleviate several inflammatory markers. The current objective was to combine methotrexate (MTX) with GNPs to overcome MTX restrictions. GNPs were fabricated by a chemical reduction technique, utilizing sodium citrate and tween 20. The MTX-GNPs formulations were characterized in vitro by \% entrapment efficiency (\%EE), particle size, polydispersity index (PDI) zeta potential, and \% release. The MTX-GNPs formulation was administrated as an intra-articular solution, and additionally, incorporated into a Carbopol gel to investigate its anti-arthritic effectiveness and bioavailability in vivo. The results indicated that a \%EE of 87.53 {\textpm} 1.10\%, and a particle size of 60.62 {\textpm} 2.41 nm with a PDI of 0.31 {\textpm} 0.03, and a zeta potential of -27.80 {\textpm} 0.36 mV were optimal. The in vitro release of MTX from the MTX-GNPs formulation demonstrated that the MTX-GNPs formulation{\textquoteright}s release was 34.91 {\textpm} 1.96\% and considerably (p \< 0.05) lower than that of free MTX, showing a significant difference in dissolution patterns (p \< 0.05). In vivo, MTX-GNPs formulations inhibited IL-6 by 36.52\%, ACCP (63.25 \%), COMP (28.16\%), and RANKL (63.67\%), as well as elevated IL-10 by 190.18\%. Transdermal MTX-GNPs decreased IL-6 by 22.52\%, ACCP (56.63\%), COMP (52.64\%), and RANKL (79.5\%), as well as increased IL-10 by 168.37\%. Histological investigation supported these recent findings. Conclusions: Marked improvements in MTX anti-arthritic effects are seen when it is conjugated to GNPs.

}, author = {Salem, Heba F and Abd El-Maboud, Marwa Mohamed and Said, Amira S A and Salem, Mohamed Nabil and Dina Sabry and Hussain, Nadia and El-Ghafar, Omnia A M Abd and Hussein, Raghda R S} } @article {228889, title = {Prophylactic Evidence of MSCs-Derived Exosomes in Doxorubicin/Trastuzumab-Induced Cardiotoxicity: Beyond Mechanistic Target of NRG-1/Erb Signaling Pathway.}, journal = {International journal of molecular sciences}, volume = {23}, year = {2022}, abstract = {

Trastuzumab (Trz) is a humanized monoclonal antibody targeting epidermal growth factor receptor 2 (HER2; ErbB2). The combined administration of Trz and doxorubicin (DOX) has shown potent anti-cancer efficacy; however, this regimen may be accompanied by severe cardiac toxicity. Mesenchymal stem cells (MSCs)-derived exosomes are nanosized vesicles that play a crucial role in cell-cell communication and have shown efficacy in the treatment of various diseases. In this study, we aim to investigate the cardioprotective effects of MSCs-derived exosomes in a DOX/Trz- mediated cardiotoxicity model, and the possible mechanisms underlying these effects are elucidated. Forty-nine male rats were randomly assigned into four groups: Group I (control); Group II (Dox/Trz); Group III (protective group); and Group IV (curative group). Cardiac hemodynamic parameters, serum markers of cardiac injury, oxidative stress indices, and cardiac histopathology were investigated. Further, transcript profile of specific cardiac tissue injury markers, apoptotic markers, and fibrotic markers were analyzed using qRT-PCR, while the protein expressions of pAkt/Akt, pERK/ERK, pJNK/JNK, pJNK/JNK, and pSTAT3/STAT3 were evaluated by ELISA. Additionally, cardiac mirR-21 and miR-26a were assessed. A combined administration of DOX/Trz disrupted redox and Ca homeostasis in cardiac tissue induced myocardial fibrosis and myofibril loss and triggered cardiac DNA damage and apoptosis. This cardiotoxicity was accompanied by decreased NRG-1 mRNA expression, HER2 protein expression, and suppressed AKT and ERK phosphorylation, while triggering JNK phosphorylation. Histological and ultra-structural examination of cardiac specimens revealed features typical of cardiac tissue injury. Moreover, a significant decline in cardiac function was observed through biochemical testing of serum cardiac markers and echocardiography. In contrast, the intraperitoneal administration of MSCs-derived exosomes alleviated cardiac injury in both protective and curative protocols; however, superior effects were observed in the protective protocol. The results of the current study indicate the ability of MSCs-derived exosomes to protect from and attenuate DOX/Trz-induced cardiotoxicity. The NRG-1/HER2, MAPK, PI3K/AKT, PJNK/JNK, and PSTAT/STAT signaling pathways play roles in mediating these effects.

}, author = {Ebrahim, Nesrine and Al Saihati, Hajir A and Ola Mostafa and Hassouna, Amira and Abdulsamea, Sameh and El Gebaly, Eman Abd El Aziz M and Abo-Rayah, Nashwa Hassan and Dina Sabry and Mohamed El-Sherbiny and Madboly, Abdelmonem G and Hussien, Noha Ibrahim and Saadani, Raja El Hasnaoui and Ebrahim, Hasnaa Ali and Badr, Omnia A M and Elsherbiny, Nehal M and Salim, Rabab F} } @article {228893, title = {The therapeutic potential of Camel Wharton jelly mesenchymal stem cells (CWJ-MSCs) in canine chronic kidney disease model.}, journal = {Stem cell research \& therapy}, volume = {13}, year = {2022}, pages = {387}, abstract = {

BACKGROUND: Chronic kidney disease (CKD) is a worldwide health problem that its incidence increases nowadays with the increase in the risk of environmental pollution. CKD can progress to end-stage renal disease (ESRD) which usually ends fatally. This study aimed to examine the therapeutic potential of Camel Wharton jelly-mesenchymal stem cells (CWJ-MSCs) in chronic kidney disease model induced in dogs.

METHODS: CWJ-MSCs were injected directed to the kidney with ultrasonographic guidance in dogs with 5/6 nephrectomy to evaluate its therapeutic potency in such cases. Analysis of variance was applied in normally distributed quantitative variables while a non-parametric Mann-Whitney test was used for non-normally distributed quantitative variables.

RESULTS: The serum urea and creatinine in the treated group were significantly decreased transferring dogs in the treated group from stage 3 to stage 2 CKD according to the IRIS staging system. Histopathology of renal tissue revealed improving CKD lesions by increasing regeneration of degenerated tubules, maintaining the integrity of glomeruli. New vascularization with blood vessels remodeling were common findings. Periodic acid Schiff stain of renal tissue showed the integrity of renal tubules and thickness of the glomerular basement membrane. Fibrosis of cortex and medulla was lower in the treated group than in the CKD model as monitored by Mallory{\textquoteright}s trichrome stain (MTC). NGAL and KIM-1 genes expression were decreased while VEGF and EGF genes expression were increased indicating renal tissue repair.

CONCLUSIONS: CWJ-MSCs have a therapeutic potential in the CKD model induced in dogs.

}, author = {Hala MF El Miniawy and Haithem A Farghali and Marwa S. Khattab and Ibrahim A. Emam and Ibrahem, Essam M. and Dina Sabry and Ismail, Tahany A.} } @article {228896, title = {Therapeutic Potential of Mesenchymal Stem Cells versus Omega n - 3 Polyunsaturated Fatty Acids on Gentamicin-Induced Cardiac Degeneration.}, journal = {Pharmaceutics}, volume = {14}, year = {2022}, abstract = {

This study compared the cardioprotective action of mesenchymal stem cells (MSCs) and PUFAs in a rat model of gentamicin (GM)-induced cardiac degeneration. Male Wistar albino rats were randomized into four groups of eight rats each: group I (control group), group II (gentamicin-treated rats receiving gentamicin intraperitoneally (IP) at dose of 100 mg/kg/day for 10 consecutive days), group III (gentamicin and PUFA group receiving gentamicin IP at dose of 100 mg/kg/day for 10 consecutive days followed by PUFAs at a dose of 100 mg/kg/day for 4 weeks), and group IV (gentamicin and MSC group receiving gentamicin IP at dose of 100 mg/kg/day followed by a single dose of MSCs (1 {\texttimes} 10)/rat IP). Cardiac histopathology was evaluated via light and electron microscopy. Immunohistochemical detection of proliferating cell nuclear antigen (PCNA), caspase-3 (apoptosis), Bcl2, and Bax expression was performed. Moreover, cardiac malonaldehyde (MDA) content, catalase activity, and oxidative stress parameters were biochemically evaluated. Light and electron microscopy showed that both MSCs and PUFAs had ameliorative effects. Their actions were mediated by upregulating PCNA expression, downregulating caspase-3 expression, mitigating cardiac MDA content, catalase activity, and oxidative stress parameters. MSCs and PUFAs had ameliorative effects against gentamicin-induced cardiac degeneration, with MSCs showing higher efficacy compared to PUFAs.

}, author = {Meligy, Fatma Y and El-Deen Mohammed, Hanan Sharaf and Tarek M. Mostafa and Elfiky, Mohamed M and El-Sayed Mohamed Ashry, Israa and Abd-Eldayem, Ahmed M and Rizk, Nermin I and Dina Sabry and Abd Allah, Eman S H and Ahmed, Salwa Fares} } @article {228891, title = {Vitamin D3 potentiates the nephroprotective effects of vildagliptin-metformin combination in a rat model of metabolic syndrome.}, journal = {Fundamental \& clinical pharmacology}, volume = {36}, year = {2022}, pages = {306-323}, abstract = {

The current study was conducted to investigate the nephroprotective effects of vildagliptin-metformin combination in an experimental model of fructose/salt-induced metabolic syndrome (MetS). A major aim was to evaluate the potential capacity of vitamin D3 to potentiate the pleiotropic nephroprotective effects of vildagliptin-metformin combination. MetS was induced in adult male Wistar rats by adding fructose (10\%) to everyday drinking water and salt (3\%) to the diet for 6~weeks. Along with the same concentrations of fructose/salt feeding, MetS rats were then treated orally with either vildagliptin (10~mg/kg/day)-metformin (200 mg/kg/day) combination, vitamin D3 (10~μg/kg/day), or the triple therapy for a further 6~weeks. The incidence of MetS was confirmed 6~weeks after fructose/salt consumption, when the rats exhibited significant weight gain, dyslipidemia, hyperuricemia, insulin resistance, hyperinsulinemia, and impaired glucose tolerance. At the end of the 12-week experimental period, MetS rats displayed significantly deteriorated renal function, enhanced intrarenal oxidative stress and inflammation together with exaggerated renal histopathological damages and interstitial fibrosis. The study has corroborated antioxidant, anti-inflammatory, and antifibrotic effects of vildagliptin-metformin combination, vitamin D3, and the triple collaborative therapy, conferring renoprotection in the setting of MetS. Due attention has been paid to the crucial role of dipeptidyl peptidase-4 inhibition and sirtuin-1/5{\textquoteright} adenosine monophosphate-activated protein kinase activation as novel therapeutic targets to optimize renoprotection. The apparent potentiating effect, evoked upon coadministration of vitamin D3 with vildagliptin-metformin combination, may provide a cornerstone for further clinical investigations.

}, author = {Wahba, Nehal S and Abdel-Ghany, Rasha H and Salah A. Ghareib and Abdel-Aal, Mohamed and Alsemeh, Amira E and Dina Sabry} } @article {207773, title = {Adipose mesenchymal stem cells combined with platelet-rich plasma accelerate diabetic wound healing by modulating the Notch pathway.}, journal = {Stem cell research \& therapy}, volume = {12}, year = {2021}, pages = {392}, abstract = {

BACKGROUND: Diabetic foot ulceration is a serious chronic complication of diabetes mellitus characterized by high disability, mortality, and morbidity. Platelet-rich plasma (PRP) has been widely used for diabetic wound healing due to its high content of growth factors. However, its application is limited due to the rapid degradation of growth factors. The present study aimed to evaluate the efficacy of combined adipose-derived mesenchymal stem cells (ADSCs) and PRP therapy in promoting diabetic wound healing in relation to the Notch signaling pathway.

METHODS: Albino rats were allocated into 6 groups [control (unwounded), sham (wounded but non-diabetic), diabetic, PRP-treated, ADSC-treated, and PRP+ADSCs-treated groups]. The effect of individual and combined therapy was evaluated by assessing wound closure rate, epidermal thickness, dermal collagen, and angiogenesis. Moreover, gene and protein expression of key elements of the Notch signaling pathway (Notch1, Delta-like canonical Notch ligand 4 (DLL4), Hairy Enhancer of Split-1 (Hes1), Hey1, Jagged-1), gene expression of angiogenic marker (vascular endothelial growth factor and stromal cell-derived factor 1) and epidermal stem cells (EPSCs) related gene ({\ss}1 Integrin) were assessed.

RESULTS: Our data showed better wound healing of PRP+ADSCs compared to their individual use after 7 and 14 days as the combined therapy caused reepithelialization and granulation tissue formation with a marked increase in area percentage of collagen, epidermal thickness, and angiogenesis. Moreover, Notch signaling was significantly downregulated, and EPSC proliferation and recruitment were enhanced compared to other treated groups and diabetic groups.

CONCLUSIONS: These data demonstrated that PRP and ADSCs combined therapy significantly accelerated healing of diabetic wounds induced experimentally in rats via modulating the Notch pathway, promoting angiogenesis and EPSC proliferation.

}, author = {Ebrahim, Nesrine and Dessouky, Arigue A and Ola Mostafa and Hassouna, Amira and Yousef, Mohamed M and Seleem, Yasmin and El Gebaly, Eman Abd El Aziz M and Allam, Mona M and Farid, Ayman Samir and Saffaf, Bayan A and Dina Sabry and Nawar, Ahmed and Shoulah, Ahmed A and Khalil, Ahmed H. and Abdalla, Sami F and Mohamed El-Sherbiny and Elsherbiny, Nehal M and Salim, Rabab F} } @article {207771, title = {Apoptotic endocrinal toxic effects of perchlorate in human placental cells.}, journal = {Toxicology reports}, volume = {8}, year = {2021}, pages = {863-870}, abstract = {

Background: Perchlorate is a strong oxidizing agent and has many adverse health effects. This study investigated the potential oxidative, apoptotic, and endocrinal toxic effects of perchlorate in human placenta-derived mesenchymal stem cells (HP-MSCs).

Methods: HP-MSCs were treated with two doses of perchlorate (5 and 15 μg/L) for three days. The perchlorate{\textquoteright}s effects were detected by histopathological examination, aromatase/CYP19 A1 activity, reactive oxygen species production (ROS), and Caspase-3 expression.

Results: The highest perchlorate concentration (15 μg/L) caused significant placental histopathological changes. The placental cell viability was significantly affected by a significant increase in ROS generation; caspase-3 expression, and a significant reduction of CYP 19 activity. Despite the slight induction effect of the lowest perchlorate concentration (5 μg/L) on caspase 3 expression, CYP 19 activity, and ROS generation, it did not affect placental cellular viability.

Conclusion: This study suggested that perchlorate could modulate aromatase activity and placental cytotoxicity. The continuous monitoring of the actual perchlorate exposure is needed and could be cost-effective.

}, author = {Mona M. Ali and Khater, Sarah A and Fayed, Amel Ahmed and Dina Sabry and Samah F. Ibrahim} } @article {207769, title = {Does Intra-Articular Injection of Platelet-Rich Plasma Have an Effect on Cartilage Thickness in Patients with Primary Knee Osteoarthritis?}, journal = {Current rheumatology reviews}, year = {2021}, abstract = {

OBJECTIVES: To determine the effect of intra-articular injection of platelet-rich plasma (PRP) in patients with primary knee osteoarthritis (OA) by clinical evaluation and ultrasonographic (US) assessment of cartilage thickness.

PATIENTS AND METHODS: A total of 100 patients with mild to severe primary knee OA using the Kellgren-Lawrence (K-L) grading scale were included and divided into two groups. Group I included 50 patients who were given two intra-articular knee injections of PRP, 1 week apart; Group II included 50 patients who received non-steroidal anti-inflammatory drugs (NSAIDs) and chondroprotective drugs. Functional assessment of all OA patients done using the basal WOMAC score, at 2 and 6 months.US assessment of femoral condylar cartilage thickness was conducted basally and at 6 months.

RESULTS: Improvement of WOMAC score was observed at 2 and 6 months in Group I following PRP injection compared to Group II (p values \< 0.001), The improvement of WOMAC in Group I occurred in all severity degrees of OA (p \< 0.001). Moreover, a significant increase in cartilage thickness at the intercondylar area (ICA) at 6 months relative to baseline assessment by US in Group I (p = 0.041) was found.

CONCLUSION: Treatment with PRP injections can reduce pain and improve knee function in patients with various degrees of articular degeneration. Further studies are needed to clarify the anabolic effect of PRP on the articular cartilage.

}, author = {Noha M Abdel Baki and Zeinab O. Nawito and Abdelsalam, Nehal M S and Dina Sabry and Elashmawy, Hossam and Seleem, Nagy A and Taha, Azza Ali Abdel-Azeem and Mohamed El Ghobashy} } @article {207774, title = {Efficacy of Photobiomodulation and Metformin on Diabetic Cell Line of Human Periodontal Ligament Stem Cells through Pathway.}, journal = {Reports of biochemistry \& molecular biology}, volume = {10}, year = {2021}, pages = {30-40}, abstract = {

Background: Diabetes mellitus (DM) is a metabolic disorder resulting from hyperglycemia. Hyperglycemia contributes to oxidative stress, and the release of advanced glycation end products (AGEs) further promotes disease pathogenesis. Uncontrolled diabetes reflects great oral complications and affects human oral health. So, the present study aimed to assess the effects of photobiomodulation therapy (PBMT) and Metformin on proliferation and viability of human periodontal ligament stem cells (HPDLSCs) cultured in high glucose medium.

Methods: HPDLSCs were collected, isolated, and characterized and then divided into eight groups. Addition of extra glucose to diabetic groups 24 hours before cell irradiations. Metformin was added to half of the diabetic groups. Cells were irradiated with 808 nm diode laser 24, 48 hours. Cell viability was analyzed with MTT assay 24 hours post-irradiation to detect cell viability in each group. Real-time (PCR) was used to evaluate gene expression of and and the effect of PBMT on Pathway. ELISA reader was used to evaluating cell viability through (ROS, TNF-α, IL-10) protein levels after cell irradiation.

Results: Photobiomodulation at 1, 2, and 3 J/cm2 combined with metformin significantly promoted diabetic cell lines of HPDLSCs viability (in MTT assay and ELISA reader of ROS, TNF-α, IL-10 results) and gene expression of , and levels (p\< 0.05).

Conclusion: photobiomodulation with 3 J/cm combined with metformin enhanced proliferation and viability of diabetic cell lines of HPDLSCs and thus could improve differentiation and function of diabetic cell lines of HPDLSCs with minimum side effects.

}, author = {Latifa Mohamed Abdelgawad and Abd El-Hamed, Manar Mohy and Dina Sabry and Abdelgwad, Marwa} } @article {207770, title = {Neuroprotective Potential of Bone Marrow-Derived Mesenchymal Stem Cells Following Chemotherapy.}, journal = {Biomedicines}, volume = {9}, year = {2021}, abstract = {

Cisplatin (CP) is extensively used in the medical oncology field for malignancy treatment, but its use is associated with neurological side effects that compromise the patients{\textquoteright} quality of life. Cytotherapy is a new treatment strategy for tissue damage that has recently emerged. The use of bone marrow-derived mesenchymal stem cells (BM-MSCs) was investigated for its therapeutic potential against CP-induced chemobrain as well as various models of brain damage. This study was carried out to elucidate, for the first time, the role of the intravenous injection (IV) of BM-MSCs against CP-induced neurotoxicity in a rat model through investigation of the parameters of oxidative stress, inflammation, and apoptosis in brain tissue. A rat model of neurotoxicity was generated by intraperitoneal injection of 7.5 mg/kg CP while 2 {\texttimes} 10 BM-MSCs was given by IV as a therapeutic dose. Injection of CP led to a significant rise in malondialdehyde and nitric oxide levels accompanied by a marked depletion of superoxide dismutase and reduced glutathione content in brain tissue in comparison to the normal control (NC) rats. Furthermore, a remarkable rise in the brain levels of inflammatory cytokines interleukin (IL)-1β and IL-6, together with the expression of apoptotic marker caspase-3, and the downregulation of the brain expression of proliferating marker Ki-67 in brain tissue were detected in the CP group compared to the NC group. Histopathological alterations were observed in the brain tissue of the CP group. BM-MSCs mitigated the biochemical and histopathological alterations induced by CP without affecting brain cell proliferation. BM-MSCs could be used as a promising neuroprotective agent against CP-induced neurotoxicity.

}, author = {Sherif, Iman O and Al-Shaalan, Nora H and Dina Sabry} } @article {207768, title = {Nicorandil prevents the nephrotoxic effect of cyclosporine-A in albino rats through modulation of HIF-1α/VEGF/eNOS signaling.}, journal = {Canadian journal of physiology and pharmacology}, volume = {99}, year = {2021}, pages = {411-417}, abstract = {

Despite that cyclosporine-A (CsA) is a widely used immunosuppressive drug, its nephrotoxic effect limits its long-term administration. Herein we tried to investigate its renal effect on endothelial dysfunction targeting the hypoxia-inducible factor (HIF-1α)~/ vascular endothelial growth factor (VEGF)~/ endothelial nitric oxide synthase (eNOS) pathway and the possible modulation by nicorandil. Eight groups of adult male Wistar rats were included: (1) control; (2) vehicle group (received oil); (3) glibenclamide 5~mg{\textperiodcentered}kg{\textperiodcentered}day administered orally; (4) nicorandil 10~mg{\textperiodcentered}kg{\textperiodcentered}day administered orally; (5) CsA 25~mg{\textperiodcentered}kg{\textperiodcentered}day administered orally; (6) combined administration of CsA and nicorandil; (7) glibenclamide was added to CsA; and (8) both CsA and nicorandil were combined with glibenclamide. The treatment continued for six~weeks. Combined nicorandil with CsA improved renal function deterioration initiated by CsA. CsA decreased the renal expression levels (~\< 0.001) of HIF-1α, eNOS, and VEGF, inducing endothelial dysfunction and triggering inflammation, and upregulated the profibrotic marker transforming growth factor (TGF-β). Nicorandil fixed the disturbed HIF-1α/VEGF/eNOS signaling. Nicorandil corrected the renal functions, confirmed by the improved histological glomerular tuft retraction that was obvious in the CsA group, without significant influence by glibenclamide. Proper protection from CsA-induced nephrotoxicity was achieved by nicorandil. Nicorandil reversed the disturbed HIF-1α/VEGF/eNOS pathway created by CsA.

}, author = {Harb, Inas A and Ashour, Hend and Dina Sabry and El-Yasergy, Dina Fawzy and Hamza, Wael Mostafa and Abeer Mostafa} } @article {228898, title = {rs62139665 Polymorphism in the Promoter Region of EpCAM Is Associated With Hepatitis C Virus-Related Hepatocellular Carcinoma Risk in Egyptians.}, journal = {Frontiers in oncology}, volume = {11}, year = {2021}, pages = {754104}, abstract = {

Hepatocellular carcinoma (HCC) is a universal health problem that is particularly alarming in Egypt. The major risk factor for HCC is hepatitis C virus (HCV) infection which is a main burden in Egypt. The epithelial cell adhesion molecule (EpCAM) is a stem cell marker involved in the tumorigenesis and progression of many malignancies, including HCC. We investigated the association of -935 C/G single nucleotide polymorphism in EpCAM promoter region (rs62139665) with HCC risk, EpCAM expression and overall survival in Egyptians. A total of 266 patients (128 HCV and 138 HCC cases) and 117 age- and sex-matched controls participated in this study. Genotyping, performed using allelic discrimination and confirmed by sequencing, revealed a significant association between EpCAM rs62139665 and HCC susceptibility, with higher GG genotype and G allele distribution in HCC patients than in non-HCC subjects. Such association was not detected in HCV patients compared to controls. EpCAM gene expression levels, determined in blood by RT-qPCR, and its serum protein expression levels, determined by ELISA, were significantly higher in GG relative to GC+CC genotype carriers in HCV and HCC patients in a recessive model. ROC analysis of EpCAM protein levels revealed significant discriminatory power between HCC patients and non-HCC subjects, with improved diagnostic accuracy when combining α-fetoprotein and EpCAM compared to that of α-fetoprotein alone. Altogether, EpCAM rs62139665 polymorphism is significantly associated with HCC and with EpCAM gene and protein expression levels in the Egyptian population. Moreover, serum EpCAM levels may hold promise for HCC diagnosis and for improving the diagnostic accuracy of α-fetoprotein.

}, author = {Motawi, Tarek Mohamed Kamal and Sadik, Nermin Abdel Hamid and Dina Sabry and Fahim, Sally Atef and Shahin, Nancy Nabil} } @article {207772, title = {Some health effects of work-related stress among nurses working in critical care units.}, journal = {Toxicology and industrial health}, volume = {37}, year = {2021}, pages = {142-151}, abstract = {

Occupational stress is a major health problem among nurses. Critical care nurses appear to experience more stress at work compared to others. Stress is associated with multiple system disorders, hormonal, and immunological disturbances, and genetic effects. The aim of our study was the detection of health effects of work-related stress and to investigate the link between stress and immune response, alterations of hormones, and expression of micro-RNA (miRNA) among critical care nurses. An exposed 80 critical care nurses matched to 80 controls were involved in our study. Full history, psychological assessment using the General Health Questionnaire (GHQ12) and a complete clinical examination were done for both groups. Serum interleukin (IL)-6, IL-10, luteinizing hormone (LH), follicle-stimulating hormone, thyroid-stimulating hormone (TSH), free triiodothyronine, and free thyroxine (FT4) were measured by enzyme-linked immunosorbent assay, micro-RNA26, and 142 extractions. The exposed group had a mean age of 41 {\textpm} 10 years old and mean work duration of 22 {\textpm} 9.7 years, matched to 80 controls. The exposed group (32.5\%) was associated with severe psychological distress (GHQ scores \> 20) compared to only 5\% among controls. In addition, the exposed group had a significantly higher level of miRNA 26, miRNA 142, TSH, LH, and IL-6 when compared to the control group. However, there a significantly lower level of FT4 among the exposed group compared to the control group, there were no statistically significant differences between the studied participants regarging FT3,FSH and IL-10 levels. Stress is prevalent among critical care nurses and is reflected on their psychological health with an increase in inflammatory cytokines and disturbances in endocrine functions.

}, author = {Eldin, A Seif and Dina Sabry and Abdelgwad, Marwa and Ramadan, Mona Abdallah} } @article {193053, title = {Comparative study on effect of mesenchymal stem cells and endothelial progenitor cells on treatment of experimental CCL4-induced liver fibrosis.}, journal = {Archives of physiology and biochemistry}, year = {2020}, pages = {1-10}, abstract = {

We speculated impacts of BM-MSCs and UC-EPCs on reversal of hepatic injury induced by carbon tetrachloride (CCl4). Fifty adult rats were divided into five groups: control group, CCl4A group, CCl4B group, CCl4/BM-MSCs group and CCl4/UC-EPCs group. Blood samples were driven to measure concentration of albumin and ALT. Quantitative expression of HGF, TGF-β, MMP-2, and VEGF were assessed by PCR. Histological and immunohistochemistry examination of the liver tissue were performed. There was elevating albumin ( \< .05) and reducing ALT ( \< .05) concentrations in groups treated with BM-MSCs and UC-EPCs compared to untreated CCL4A\&B groups. UC-EPCs treated group have significantly higher MMP-2 and VEGF ( \< .01) genes expression than BM-MSCs treated group. Furthermore, UC-EPCs were more valuable than BMMSCs in increasing gene expression of HGF ( \< .05) and immunohistochemistry of α-SMA and Ki-67 ( \< .01). BM-MSCs have significantly lower TGF-β ( \< .00) compared to UC-EPCs. This study highlighted on liver regeneration role of both UC-EPCs and BM-MSCs in liver fibrosis.

}, author = {Abdelgwad, Marwa and Manal Ewaiss and Dina Sabry and Warda A. Khalifa and Altaib, Zeinab M and Maha alhelf} } @article {193055, title = {The effect of exosomes derived from mesenchymal stem cells in the treatment of induced type 1 diabetes mellitus in rats.}, journal = {Biotechnology letters}, year = {2020}, abstract = {

AIM: The aim of the current study was to evaluate the therapeutic and regenerative effects of MSCs derived exosomes in the treatment of type 1 DM and to compare its effects with MSCs themselves. The experiment was done on forty albino rats grouped as follows, group (1): Ten healthy rats, group (2): Ten induced type 1 DM rats, group (3): Ten induced type 1 DM rats received exosomes intraperitoneally, and group (4): Ten induced type 1 DM rats received MSCs intraperitoneally. Serum glucose and plasma insulin levels were assessed weekly. QRT-PCR was done to assess regeneration of pancreatic beta cells by measuring insulin, Pdx1, Smad2, Smad3 and TGFβ genes. Additionally, histopathological and immune-histochemical examinations were done to confirm pancreatic tissue regeneration.

RESULTS: Regarding the assessed genes (insulin, Pdx1, Smad2, Smad3 and Tgfβ) gene expression in MSCs treated group showed significant increase compared to diabetic group (p value \< 0.001) and gene expression in exosomes treated group was increased significantly compared to diabetic and MSCs treated groups (p value \< 0.001). Histopathological and immune-histochemical examination revealed regeneration of pancreatic islets in both treated groups.

CONCLUSION: MSCs Derived exosomes showed superior therapeutic and regenerative results than MSCs themselves.

}, author = {Dina Sabry and Marzouk, Samar and Zakaria, Reem and Heba A. Ibrahim and Mai Samir} } @article {193052, title = {Functional and histological evaluation of bone marrow stem cell-derived exosomes therapy on the submandibular salivary gland of diabetic Albino rats through TGFβ/ Smad3 signaling pathway.}, journal = {Heliyon}, volume = {6}, year = {2020}, pages = {e03789}, abstract = {

Background: To prevail over diabetes mellitus and its numerous complications, researchers are seeking new therapies. Exosomes are natural cargo of functional proteins and can be used as a therapeutic delivery of these molecules.

Objective: The aim of this study was to evaluate the effect of exosomes derived from bone marrow mesenchymal stem cells (BM-MSCs) as a therapeutic intervention in salivary gland diabetic complications.

Methods: Ten adult healthy male Albino rats, weighing about 150-200 g were grouped into 2 groups. Diabetic group I: consisted of 5 streptozotocin (STZ)-induced diabetic rats. Exosomes treated group II: consisted of 5 STZ-induced diabetic rats, each animal received a single injection of exosomes (100 μg/kg/dose suspended in 0.2 ml PBS) through the tail vein. All animals were sacrificed after 5 weeks from the beginning of the experiment. Submandibular salivary gland samples were excised and processed for histological, ultrastructural examination and PCR for TGFβ, Smad2 and Smad3. Blood glucose level was monitored weekly, salivary IgA and serum amylase were evaluated before and after diabetes induction and at the end of the experiment.

Results: Histological and ultrastructural results of the exosomes treated group were promising regarding the glandular and ductal elements with less fibrosis observed. Results of PCR supported the role of exosomes to inhibit the diabetic sequalae in salivary gland and its complications through inhibiting TGFβ and its related pathway via Smad2 and Smad3. Blood glucose levels were reduced. In addition, salivary glands{\textquoteright} function was improved as evidenced by reduction in serum amylase and salivary IgA.

Conclusion: BM-MSC-derived exosomes could be a novel therapeutic strategy for diabetic complications involving salivary glands.

}, author = {Nermeen AbuBakr and Tahany Haggag and Dina Sabry and Zeinab A. Salem} } @article {193049, title = {Occupational metals exposure and cognitive performance among foundry workers using tau protein as a biomarker.}, journal = {Neurotoxicology}, volume = {76}, year = {2020}, pages = {10-16}, abstract = {

INTRODUCTION: Human exposure to heavy metals is a potential risk for developing cognitive impairment. Aluminum (Al) foundry is one of industries that involve occupational exposure to different metals.

AIM OF THE WORK: to evaluate the cognitive performance of Aluminum foundry workers in relation to different metals exposure.

MATERIALS AND METHODS: a cross sectional study conducted on 75 Al foundry workers and 75 non-occupationally exposed subjects as controls. Personal interview with specially designed questionnaire, Assessment of cognitive functions done using Montreal cognitive assessment (MocA), Stress, depression and sleep were also assessed. Serum levels of Aluminum (AL), Lead (Pb), manganese (Mn), Zinc (Zn) and tau protein were measured.

RESULTS: Exposed group showed significant increase in serum levels of Aluminum, lead, Manganese and tau protein, p value \< 0.005 (mean {\textpm} SD 0.56 {\textpm} 0.18, 22.3 {\textpm} 5.01, 42.04 {\textpm} 7.4, 1.53 {\textpm} 0.58 Vs 0.36 {\textpm} 0.11, 13.4 {\textpm} 1.29, 39.4 {\textpm} 4.4, 1.03 {\textpm} 0.44 respectively) with significant decrease of zinc level compared to control (mean {\textpm} SD 46.4 {\textpm} 5.2 Vs 88.8 {\textpm} 6.04, p value 0.005). There was a significant decrease MocA scores among exposed population, (mean {\textpm} SD 24.4 {\textpm} 3.4 compared to 28.4 {\textpm} 1.3 in non exposed, p value \< 0.005). which was affected by serum levels of lead, aluminum, manganese and tau protein (β -0.165, -8.958, -.286, -2.341 respectively and p \< 0.005).Stress scores was higher in exposed workers than control but not affecting cognitive performance.

CONCLUSION: occupational exposure to metals can cause cognitive dysfunction which may be subtle, so there is a need for formal cognitive testing at baseline, and on regular intervals during working period. Serum tau protein could be used as a prognostic biomarker for the hazardous effect of occupational exposure to these metals on the neuronal cells.

}, author = {Mohammed, Rateba S and Walaa Ibrahim and Dina Sabry and Shaimaa Ibrahim El-Jaafary} } @article {193051, title = {A potential association between psoriasin to rs4819554 of IL-17RA gene polymorphism in psoriasis Egyptian patients.}, journal = {Archives of dermatological research}, volume = {312}, year = {2020}, pages = {273-281}, abstract = {

Interleukin 17 (IL-17) is one of the pro-inflammatory cytokine. Psoriasin is a noticeably over-expressed protein found in the skin lesions of psoriatic patients. Our current study was planned to examine the association of (- 947 A/G) single nucleotide polymorphism (SNP) in IL-17RA promoter region (rs4819554) with psoriasis susceptibility in Egyptian psoriatic patients. Our study included 100 patients and 100, age as well as sex matched, control groups. IL-17RA SNP association was studied using allelic discrimination. RT-qPCR and ELISA were done to assess IL-17 expression. ELISA was performed to assess psoriasin expression. Our study showed a significant association between IL-17 rs4819554 SNP and psoriasis risk, evidenced by higher G allele and AG genotype frequencies in psoriatic patients when compared to controls (allelic: OR 2.283, 95\% CI 1.321-3.946, p = 0.003, and genotype: OR 3.026, 95\% CI 1.356-6.752, p = 0.007). Additionally, serum psoriasin level was significantly increased when comparing psoriatic patients to controls (p = 0.0003). Moreover, significant increase in IL 17 gene and protein level in AA, AG psoriatic genotypes compared to the corresponding genotypes in normal control (p = 0.0004). IL-17 rs4819554 is significantly associated with psoriasis, and with psoriasin level, in the Egyptian population.

}, author = {Dina Sabry and Aboraia, Nesreen and Mai Samir} } @article {193054, title = {Predictors of recurrence and survival of hepatocellular carcinoma: A prospective study including transient elastography and cancer stem cell markers.}, journal = {Arab journal of gastroenterology : the official publication of the Pan-Arab Association of Gastroenterology}, volume = {21}, year = {2020}, pages = {95-101}, abstract = {

BACKGROUND AND STUDY AIMS: To investigate whether the measurement of liver stiffness (LSM) using fibroscan and the serum Cancer Stem Cells (CSC): Ep-CAM and cytokeratin-19, could predict the recurrence of hepatocellular carcinoma (HCC) and their impact on clinical outcome and overall survival.

PATIENTS AND METHODS: This is a prospective study, including 179 HCV-related HCC patients. All patients were treated following the BCLC guidelines. All HCC patients had transient elastography, measurements of Ep-CAM and cytokeratin-19 before and six months post-treatment. We looked for predictors of recurrence and performed a survival analysis using Kaplan-Meier estimates.

RESULTS: TACE was the most common procedure (77.1\%), followed by microwave ablation (15.6\%). Complete ablation was achieved in 97 patients; 55 of them developed HCC recurrence. After treatment, LSM increased significantly with a significant reduction in CSCs levels in complete and partial response groups. The median time to observe any recurrence was 14~months. LSM increased significantly post-treatment in patients with recurrence versus no recurrence. Higher levels of CSCs were recorded at baseline and post-treatment in patients with recurrence but without statistical significance. We used univariate analysis to predict the time of recurrence by determining baseline CK-19 and platelet levels as the key factors, while the multivariate analysis determined platelet count as a single factor. The univariate analysis for prediction of overall survival included several factors, LSM and EpCAM (baseline and post-ablation) among them, while multivariate analysis included factors such as Child score B and incomplete ablation.

CONCLUSION: Dynamic changes were observed in LSM and CSCs levels in response to HCC treatment and tumour recurrence. Child score and complete ablation are factors that significantly affect survival.

}, author = {Hend Ibrahim Shousha and Fouad, Rabab and Tamer Mahmoud Elbaz and Dina Sabry and Mohamed Mahmoud Nabeel and Ahmed Hosni Abdelmaksoud and Aisha Mahmoud El Sharkawy and Soliman, Zeinab Abdellatif and Ghada Habib and Ashraf Omar Abdelaziz} } @article {193047, title = {Preparation and appraisal of self-assembled valsartan-loaded amalgamated Pluronic F127/Tween 80 polymeric micelles: Boosted cardioprotection regulation of Mhrt/Nrf2 and Trx1 pathways in cisplatin-induced cardiotoxicity.}, journal = {Journal of drug targeting}, volume = {28}, year = {2020}, pages = {282-299}, abstract = {

This study aimed to develop valsartan (VAL)-loaded mixed micelles and investigate their cardioprotective potential and molecular mechanisms through Mhrt/Nrf2 and Trx1 pathways. VAL-loaded mixed micelles have not been elaborated and their impact on Mhrt/Nrf2 and Trx1 pathways has not been yet inspected. VAL-loaded mixed micelles were prepared, incorporating Pluronic F127 and Tween 80, adopting thin-film hydration method. The micelles were evaluated for drug entrapment efficiency, loading characteristics, particle size, morphology, drug release and micelles storage stability. The pharmacokinetic studies were explored in rats. Also, VAL suspension and mixed micelles were tested in cisplatin-induced cardiotoxicity in rats either pre to or simultaneously with cisplatin. RNA expression of lnc Mhrt and protein expression of Nrf2, Trx1, Ask1, AMPK and caspase 3, oxidative stress and cardiac injury markers besides tailed DNA\% by comet assay were assessed. Pharmacokinetic studies evoked a 3.75-fold increase in oral bioavailability as compared with VAL suspension. Overall, treatment with VAL-loaded mixed micelles was superior to VAL suspension in decreasing oxidative stress and cardiac injury markers and restoring the abnormalities occurred in Mhrt/Nrf2 and Trx1 pathways. Thus, mixed micelles would be promising nanocarrier for the engineering of VAL with reinforced pharmacokinetics and cardioprotection characteristics.

}, author = {Aboud, Heba M and Mahmoud, Mohamed O and Abdeltawab Mohammed, Marwa and Shafiq Awad, Mohammad and Dina Sabry} } @article {193050, title = {The prospective role of mesenchymal stem cells exosomes on circumvallate taste buds in induced Alzheimer{\textquoteright}s disease of ovariectomized albino rats: (Light and transmission electron microscopic study).}, journal = {Archives of oral biology}, volume = {110}, year = {2020}, pages = {104596}, abstract = {

OBJECTIVE: To elucidate the effect of Alzheimer{\textquoteright}s disease on the structure of circumvallate papilla taste buds and the possible role of exosomes on the taste buds in Alzheimer{\textquoteright}s disease.

DESIGN: Forty two ovariectomized female adult albino rats were utilized and divided into: Group I: received vehicle. Group II: received aluminum chloride to induce Alzheimer{\textquoteright}s disease. Group III: after the induction of Alzheimer{\textquoteright}s disease, each rat received single dose of exosomes then left for 4 weeks. The circumvallate papillae were prepared for examination by light and transmission electron microscope.

STATISTICAL ANALYSIS: histomorphometric data were statistically analyzed.

RESULTS: Histological examination of circumvallate papilla in Group I showed normal histological features. Group II revealed distorted features. Group III illustrated nearly normal histological features of circumvallate. Silver impregnation results showed apparently great number of heavily impregnated glossopharyngeal nerve fibers in both Groups I \& III but markedly decreased in Group II. Synaptophysin-immunoreactivity was strong in Group I, mild in Group II and moderate in Group III. The ultra-structural examination of taste bud cells revealed normal features in Group I, distorted features in Group II and almost normal features in Group III. Statistically highest mean of Synaptophysin-immunoreactivity area\% was for Group I, followed by Group III, and the least value was for Group II.

CONCLUSIONS: Alzheimer{\textquoteright}s disease has degenerative effects. Bone marrow mesenchymal stem cell (BM-MSC)-derived exosomes have the ability to improve the destructive changes induced by Alzheimer{\textquoteright}s disease.

}, author = {Hassan, Rabab and Amany A. Rabea and Alyaa Ragae and Dina Sabry} } @article {193048, title = {Sequential peeling as a monotherapy for treatment of milder forms of acne vulgaris.}, journal = {Journal of cosmetic dermatology}, volume = {19}, year = {2020}, pages = {1381-1387}, abstract = {

BACKGROUND: Glycolic acid (GA) and salicylic acid (SA) peels have been used separately for acne treatment, not as a sequential peel.

AIM: To evaluate the efficacy and safety of sequential peeling with 70\% GA and 20\% SA as a monotherapy and as an adjuvant to systemic doxycycline in treatment of mild to moderate acne and the effect on serum interleukin (IL) 17 and tissue IL-1α.

PATIENTS/METHODS: Forty-five mild to moderate acne vulgaris patients were randomly assigned into three groups. Group [A] underwent sequential application of 70\% GA followed by 20\% SA biweekly for three months. Group [B] underwent sequential peeling and doxycycline PO100~mg BD for 1~month followed by 100 OD for 2~months. Group [C] received oral doxycycline. Acne grading, lesion counting, and patient satisfaction were assessed. Serum samples and perilesional skin biopsies were obtained at onset and 2~weeks after finishing the treatment for assessment of serum IL-17 and tissue IL-1α.

RESULTS: All groups showed statistically significant decrease in acne grading and lesion count, increase in patient satisfaction, and decrease in serum IL-17 and tissue IL-1 α after treatment. There was no significant difference between the 3 groups before or after treatment, except regarding patient satisfaction after treatment, which was significantly higher in groups [A] and [B] than group [C] (P~=~.001).

CONCLUSIONS: This study recommends using sequential GA 70\% and SA 20\% peels in the treatment of mild or moderate acne vulgaris as a new cost-effective mode, with low-down time and potential safety, in noncompliant patients on medical therapy.

}, author = {Zayed, Amira Amin and Sobhi, Rehab Mohamed and El Aguizy, Randa Mohamed Saleh and Dina Sabry and Mahmoud, Sara Bahaa} } @article {168158, title = {Association of NKX2-5, GATA4, and TBX5 polymorphisms with congenital heart disease in Egyptian children.}, journal = {Molecular genetics \& genomic medicine}, year = {2019}, month = {2019 Mar 04}, pages = {e612}, abstract = {

BACKGROUND: Several genes encoding transcription factors are known to be the primary cause of congenital heart disease. NKX2-5 and GATA4 were the first congenital heart disease-causing genes identified by linkage analysis. This study designed to study the association of five single-nucleotide variants of NKX2-5, GATA4, and TBX5 genes with sporadic nonsyndromic cases of a congenital cardiac septal defect in Egyptian children.

METHODS: Venous blood samples from 150 congenital heart disease children (including a ventricular septal defect, atrial septal defect, tetralogy of Fallot, and patent ductus arteriosus) and 90 apparently healthy of matched age and sex were studied by polymerase chain reaction followed by direct sequencing in order to study two single-nucleotide variants of NKX2-5 (rs2277923, rs28936670), two single-nucleotide variants of GATA4 (rs368418329, rs56166237) and one single-nucleotide variant TBX5 (rs6489957). The distribution of genotype and allele frequency in the congenital heart diseases (CHD) group and control group were analyzed.

RESULTS: We found different genotype frequencies of the two variants of NKX2-5, as CT genotype of rs2277923 was present in 58\% and 36\% in cases and control respectively, and TT genotype present in 6\% of the cases. Also regarding missense variant rs28936670, heterozygous AG presented in 82\% of the cases. Also, we observed a five prime UTR variant rs368418329, GT (42\% of the cases) and GG (46\% of the cases) genotypes showed the most frequent presentation in cases. While regarding a synonymous variant rs56166237, GT and GG were the most presented in cases (41.4\%, 56\% respectively) in contrast to control group (20\%, 1.7\% respectively). Also, a synonymous variant in TBX5, the distribution of genotype frequency was significantly different between the CHD group and control group. CT genotype of TBX5 -rs6489957 was found in 12 ASD, 24 VSD, six PDA, three aortic coarctation and nine fallot that represent 42\% of the cases.

CONCLUSIONS: Significantly higher frequency of different allelle of five variants was observed in cases when compared to the control group, with significant risky effect for the development of septal defect. In addition to two polymorphisms of NKX2-5 (rs2277923, rs28936670) variant~in the cardiac septal defect, two variants in GATA4 (rs368418329, rs56166237) and one variant in TBX5 (rs6489957) seem to have a role in the pathogenesis of congenital heart disease.

}, issn = {2324-9269}, doi = {10.1002/mgg3.612}, author = {Behiry, Eman G and Al-Azzouny, Mahmoud A and Dina Sabry and Behairy, Ola G and Salem, Nessrine E} } @article {168148, title = {Serum L-carnitine and vitamin D levels may be low among oral sildenafil citrate non-responders.}, journal = {International journal of impotence research}, volume = {31}, year = {2019}, month = {2019 Mar}, pages = {85-91}, abstract = {

This cross-sectional comparative study aimed to compare serum L-carnitine and 25(OH)D levels between men with ED non-responding for oral sildenafil citrate and healthy volunteers. Overall, 192 men, recruited from two University Hospitals, were allocated into two equal groups of matched age; healthy potent men and men with ED non-responders for oral sildenafil citrate. Oral sildenafil citrate non-responders self-reported inadequate erectile responses after four attempts using 100 mg with the manufacturer{\textquoteright}s guidelines relative to meals, associated medications, and sexual stimulation/arousal. Exclusion criteria were: diabetes, cardiovascular disorders, beta blockers treatment, morbid obesity, thyroid disorders, post-radical prostatectomy, and hepatic/renal failure. All participants were subjected to; history taking, clinical examination, validated IIEF-5 questionnaire, estimation of serum L-carnitine by calorimetric method and serum 25(OH)D by ELISA method. Compared with potent controls, ED men non-responders for oral sildenafil citrate showed significant decreases in the mean serum L-carnitine level (16.8 {\textpm} 3.6 uM/L versus 66.3 {\textpm} 11.9 uM/L, P = 0.001), the mean serum 25(OH)D level (21.2 {\textpm} 7.1 ng/ml versus 54.6 {\textpm} 7.9 ng/mL, P = 0.001) and IIEF-5 score (7.8 {\textpm} 2.6 versus 23.9 {\textpm} 1.3). Serum L-carnitine showed significant positive correlation with IIEF-5 scores (r = 0.873, P = 001), serum 25(OH)D (r = 0.796, P = 0.001) and significant negative correlation with the age (r = -0.515, P = 0.001). Serum 25(OH)D showed significant positive correlation with IIEF-5 scores (r = 0.855, P = 0.001) and significant negative correlation with the age (r = -0.223, P = 0.005). It is concluded that normal homeostasis of serum L-carnitine and 25(OH)D play a role in male sexual health being significantly decreased in ED non-responding for oral sildenafil citrate.

}, issn = {1476-5489}, doi = {10.1038/s41443-018-0036-4}, author = {Taymour Mostafa and Laila A. Rashed and Sabry, Dina A and Osman, Ihab and Nabil, Nashaat and Kareem, Firas and Mostafa, Ingi A} } @article {168157, title = {Adipose Tissue-Derived Mesenchymal Stem Cell Modulates the Immune Response of Allergic Rhinitis in a Rat Model.}, journal = {International journal of molecular sciences}, volume = {20}, year = {2019}, month = {2019 Feb 18}, abstract = {

This study was designed to investigate the potential effects and underlying mechanism of adipose tissue-derived mesenchymal stem cells (MSCs) on allergic inflammation compared to Montelukast as an antileukotriene drug in a rat model of allergic rhinitis (AR). The effect of MSCs was evaluated in albino rats that were randomly divided into four (control, AR, AR + Montelukast, and AR + MSCs) groups. Rats of AR group were sensitized by ovalbumin (OVA) and then challenged with daily nasal drops of OVA diluted in sterile physiological saline (50 μL/nostril, 100 mg/mL, 10\% OVA) from day 15 to day 21 of treatment with/without Montelukast (1 h before each challenge) or MSCs I/P injection (1 {\texttimes} 10$^{6}$ MCSs; weekly for three constitutive weeks). Both Montelukast and MSCs treatment started from day 15 of the experiment. At the end of the 5th week, blood samples were collected from all rats for immunological assays, histological, and molecular biology examinations. Both oral Montelukast and intraperitoneal injection of MSCs significantly reduced allergic symptoms and OVA-specific immunoglobulin E (IgE), IgG1, IgG2a and histamine as well as increasing prostaglandin E2 (PGE2). Further analysis revealed that induction of nasal innate cytokines, such as interleukin (IL)-4 and TNF-α; and chemokines, such as CCL11 and vascular cell adhesion molecule-1 (VCAM-1), were suppressed; and transforming growth factor-β (TGF-β) was up-regulated in Montelukast and MSCs-treated groups with superior effect to MSCs, which explained their underlying mechanism. In addition, the adipose tissue-derived MSCs-treated group had more restoring effects on nasal mucosa structure demonstrated by electron microscopical examination.

}, issn = {1422-0067}, doi = {10.3390/ijms20040873}, author = {Ebrahim, Nesrine and Mandour, Yasser Mohammad Hassan and Farid, Ayman Samir and Nafie, Ebtesam and Mohamed, Amira Zaky and Safwat, Miriam and Radwa Taha and Dina Sabry and Sorour, Safwa M and Refae, Ahmed} } @article {168156, title = {Anti-proliferative and anti-apoptotic potential effects of epigallocatechin-3-gallate and/or metformin on hepatocellular carcinoma cells: in vitro study.}, journal = {Molecular biology reports}, year = {2019}, month = {2019 Feb 01}, abstract = {

The effects of epigallocatechin-3-gallate (EGCG) and metformin single treatment have been tested against hepatocellular carcinoma (HCC). This study aimed to assess the combination effects of EGCG and metformin on proliferation and apoptosis of HepG2cells and identified new potential molecular targets. The effect of EGCG and metformin against cell proliferation in HepG2 was determined using MTT assay. Reverse transcription polymerase chain reaction was applied to examine the gene expression of cyclin D1, lncRNA-AF085935, caspase-3, survivin and VEGF. The level of protein expression of glypican-3 was assessed by western blot. In HepG2 cells, EGCG and metformin combination treatment exhibited high significant effect against tumor proliferation. It significantly reduced cyclin D1, lncRNA-AF085935, glypican-3 and promoted apoptosis through increasing caspase3 and decreasing survivin compared to control cells. Moreover, EGCG and metformin treated cells showed decreased expression levels of VEGF. Our study provided new insights of the anticarcinogenic effects of EGCG and metformin on HCC through their effects on glypican-3 and lncRNA-AF085935.

}, issn = {1573-4978}, doi = {10.1007/s11033-019-04653-6}, author = {Dina Sabry and Abdelaleem, Omayma O and El Amin Ali, Amani M and Rehab A. Mohammed and Abdel-Hameed, Nehal D and Hassouna, Amira and Warda A. Khalifa} } @article {168150, title = {Role of miRNA-210, miRNA-21 and miRNA-126 as diagnostic biomarkers in colorectal carcinoma: impact of HIF-1α-VEGF signaling pathway.}, journal = {Molecular and cellular biochemistry}, year = {2019}, month = {2018 Oct 24}, abstract = {

Colorectal cancer (CRC) is a major cause of death worldwide. Novel non-invasive, high diagnostic value screening test is urgently needed to improve survival rate, treatment and prognosis. Stable, small, circulating microRNA (miRNA) offers unique opportunities for the early diagnosis of several diseases. It acts as tumor oncogenes or suppressors and involve in cell death, survival, and metastasis. Communication between miRNA and carcinogenesis is critical but it still not clear and needs further investigation. The aim of our study is to evaluate the role of miR-210, miR-21, miR-126, as non-invasive diagnostic biomarkers for screening, early detection of CRC, studying their correlation with prognostic variables, and clarifying the roles of miRNAs on HIF-1α-VEGF signaling pathway. The expression of miR-210, miR-21 and miR-126 was performed using qRT-PCR in adenocarcinoma (no = 35), adenomas (no = 51), and neoplasm free controls (no = 101). Serum levels of VEGF and HIF-1α was determined by ELISA Kit. The results show that the expression of miR-210, miR-21, VEGF, HIF-1α was significantly up-regulated while that miRNA-126 was down-regulated in both adenocarcinoma and adenomas compared with controls (p \< 0.001 for each). No significant difference was noted comparing patients with adenocarcinoma and adenomas. The three miRNAs correlated with VEGF, HIF-α. The miR-210 and miR-21 associated with TNM classification and clinical staging of adenocarcinoma (p \< 0.001) and they show high diagnostic value with sensitivity and specificity 88.6\%, 90.1\% and 91.4\%, 95.0\% respectively. Our study revealed that circulating miR-210, miR-21 were up-regulated while miR-126 was down-regulated in CRC and adenomas patients, they all correlated with TNM staging and they had high diagnostic value. HIF-1α VEGF signaling pathways regulated by miRNAs played a role in colon cancer initiation. To the best of our knowledge, this is the first study of this miRNAs panel in CRC in our community. These data suggested that these biomarkers could be a potential novel, non-invasive marker for early diagnosis, screening and predicting prognosis of CRC. Understanding the molecular functions by which miRNAs affect cancer and understanding its roles in modulating the signaling output of VEGF might be fruitful in reducing the incidence and slowing the progression of this dark malignancy.

}, issn = {1573-4919}, doi = {10.1007/s11010-018-3462-1}, author = {Dina Sabry and El-Deek, Sahar E M and Maher, Moataz and El-Baz, Mona A H and El-Bader, Hala M and Eman Amer and Elham A. Hassan and Wael Fathy and El-Deek, Heba E M} } @article {168154, title = {Secreted protein acidic and rich in cysteine gene variants: Impact on susceptibility and survival of hepatocellular carcinoma patients.}, journal = {Journal of gastroenterology and hepatology}, year = {2019}, month = {2018 Nov 13}, abstract = {

BACKGROUND AND AIM: Secreted protein acidic and rich in cysteine (SPARC) is a glycoprotein involved in extracellular matrix remodeling, which regulates cell growth. It could be involved in hepatic fibrogenesis related to chronic inflammations, hepatocellular carcinoma (HCC) angiogenesis, and tumor progression. We aimed to study the expressions of single nucleotide polymorphisms in the SPARC gene and their impact on susceptibility and survival of HCC patients.

METHODS: We conducted a case-control study on 200 HCC patients and 50 matched healthy controls. All patients were subjected to laboratory investigations, ultrasound, and real-time polymerase chain reaction to detect the genetic polymorphisms (rs3210714, rs11950384, and rs7719521) in the SPARC gene in the blood.

RESULTS: One hundred sixty (80\%) patients were men with a mean age of 43~years. The SPARC gene showed a significant higher prevalence of rs3210714 mutation (i.e. AA or AG) and a significant lower prevalence of rs11950384 mutation (i.e. AA or AC) among HCC patients in comparison with controls (83\% vs 22\%, P~<=~0.001) and (65.5 vs 86\%, P~=~0.005), respectively, while rs7719521 mutation did not reach significance. On univariate and multivariate analyses, elder age and having at least one copy of the mutant rs3210714 were associated with a significantly increased risk of HCC (P~\<~0.001 for both), whereas the presence of at least one copy of the mutant rs11950384 carried a significantly reduced risk of having HCC (P~\<~0.01). Overall survival did not differ significantly between any of the SPARC gene mutation groups.

CONCLUSIONS: The SPARC gene polymorphisms had a diverse impact on the susceptibility of HCC due to its ability to inhibit or promote tumor progression. SPARC gene polymorphisms were not related to survival of our HCC patients, and probably, this needs further analysis of other SPARC gene nucleotides.

}, issn = {1440-1746}, doi = {10.1111/jgh.14541}, author = {Samar Kamal Darweesh and Abd Alziz, Rasha Ahmed and Heba Omar and Dina Sabry and Wael Fathy} } @article {193038, title = {The Effect of Mesenchymal Stem Cells Derived Microvesicles on the Treatment of Experimental CCL4 Induced Liver Fibrosis in Rats.}, journal = {International journal of stem cells}, volume = {12}, year = {2019}, pages = {400-409}, abstract = {

Background and Objectives: The release of microvesicles (MVs) from mesenchymal stem cells (MSCs) has been implicated in intercellular communication, and may contribute to beneficial paracrine effects of stem cell-based therapies. We investigated the effect of administration of MSC-MVs on the therapeutic potential of carbon tetrachloride (CCL) induced liver fibrosis in rats.

Methods: Our work included: isolation and further identification of bone marrow MSC-MVs by transmission electron microscopy (TEM). Liver fibrosis was induced in rats by CCl4 followed by injection of prepared MSC-MVs in injured rats. The effects of MSC-MVs were evaluated by biochemical analysis of liver functions, RNA gene expression quantitation for collagen-1, transforming growth factor (TGF-), interleukin-1 (IL-1), vascular endothelial growth factor (VEGF) by real time reverse transcription PCR (RT-PCR) techniques. Finally histopathological examination of the liver tissues was assessed for all studied groups.

Results: BM-MSC-MVs treated group showed significant increase in serum albumin levels, VEGF quantitative gene expression (p\<0.05), while it showed a significant decrease in serum alanine transaminase (ALT) enzyme levels, quantitative gene expression of TGF-, collagen-1, IL-1 compared to CCL fibrotic group (p\<0.05). Additionally, the histopathological assessment of the liver tissues of BM-MSC-MVs treated group showed marked decrease in the collagen deposition \& improvement of histopathological picture in comparison with CCL fibrotic group.

Conclusions: Our study demonstrates that BM-MSC-MVs possess anti-fibrotic, anti-inflammatory, and pro-angiogenic properties which can promote the resolution of CCL induced liver fibrosis in rats.

}, author = {Dina Sabry and Abbas Mohamed and Monir, Manar and Heba A. Ibrahim} } @article {193039, title = {The expression of long non coding RNA genes is associated with expression with polymorphisms of HULC rs7763881 and MALAT1 rs619586 in hepatocellular carcinoma and HBV Egyptian patients.}, journal = {Journal of cellular biochemistry}, volume = {120}, year = {2019}, pages = {14645-14656}, abstract = {

Long noncoding RNAs (lncRNAs), highly upregulated liver cancer (HULC), metastasis-associated lung adenocarcinoma transcript 1 (MALAT1), lncRNA-AF085935, and lncRNA-uc003wbd have been implicated in hepatocellular carcinoma (HCC). Single-nucleotide polymorphism (SNP) in HULC and MALAT1 are associated with HCC susceptibility. However, association between these SNPs and lncRNA-AF085935 and lncRNA-uc003wbd expression and their potential clinical value in differentiating HCC from both hepatitis B virus (HBV)-infected Egyptian patients and the healthy specimens have not been explored yet. In the present study, SNPs rs7763881 in HULC and rs619586 in MALAT1 were genotyped in 70 HBV-positive HCC, 70 HBV patients, and 70 healthy controls in Egyptian population and the level of serum lncRNA-AF085935 and lncRNA-uc003wbd of all the subjects was assayed by quantitative real-time polymerase chain reaction. HULC rs7763881 AC/CC genotype was significantly associated with decreased HCC risk. Similarly, AG/GG of MALAT1 rs619586 was associated with decreased HCC risk with a borderline significance. Serum lncRNA-AF085935 and lncRNA-uc003wbd levels were upregulated in HBV-positive HCC and HBV patients vs controls and discriminated these groups by receiver operating characteristic analysis. Patients carrying AC/CC genotype of rs7763881 and AG/GG of rs619586 had lower serum lncRNA-AF085935 and lncRNA-uc003wbd levels compared with AA genotype. In conclusion, genetic variants of lncRNA HULC and lncRNA MALAT1 are associated with the decreased susceptibility to HCC in HBV-persistent carriers and are correlated with serum lncRNA-AF085935 and lncRNAuc003wbd levels, two potential noninvasive diagnostic biomarkers for HCC.

}, author = {Tarek M. K. Motawi and Shohda A. EL-Maraghy and Dina Sabry and Mehana, Noha A} } @article {193043, title = {Extract Alleviates Methotrexate-Induced Renal Injury: New Impact on PI3K/Akt/mTOR Signaling and MALAT1 Expression.}, journal = {Biomolecules}, volume = {9}, year = {2019}, abstract = {

Renal injury induced by the chemotherapeutic agent methotrexate (MTX) is a serious adverse effect that has limited its use in the treatment of various clinical conditions. The antioxidant activity of extract (GB) was reported to mitigate renal injury induced by MTX. Our research was conducted to examine the nephroprotective role of GB versus MTX-induced renal injury for the first time through its impact on the regulation of phosphatidylinositol 3-kinase/protein kinase B/ mammalian target of rapamycin (PI3K/Akt/mTOR) signaling together with the renal level of TGF-β mRNA and long non-coding RNA-metastasis-associated lung adenocarcinoma transcript-1 (MALAT1) expression. A group of adult rats was intraperitoneally (ip) injected with MTX 20 mg/kg as a single dose to induce kidney injury (MTX group). The other group of rats was orally administered with GB 60 mg/kg every day for 10 days (GB+ MTX group). The MTX increased the serum creatinine and urea levels, renal TGF-β mRNA and MALAT1 expression, in addition to dysregulation of the PI3K/Akt/mTOR signaling when compared with normal control rats that received saline only (NC group). Moreover, renal damage was reported histopathologically in the MTX group. The GB ameliorated the renal injury induced by MTX and reversed the changes of these biochemical analyses. The involvement of PI3K/Akt/mTOR signaling and downregulation of TGF-β mRNA and MALAT1 renal expressions were firstly reported in the nephroprotective molecular mechanism of GB versus MTX-induced renal injury.

}, author = {Sherif, Iman O and Al-Shaalan, Nora H and Dina Sabry} } @article {193041, title = {In Vitro Characterization and Evaluation of Silver Nanoparticles Cytotoxicity on Human {\textquotedblleft}Liver and Breast{\textquotedblright} Cancer Cells Versus Normal Melanocytes }, journal = {The Egyptian journal of histology}, volume = {42}, year = {2019}, pages = {755-766}, author = {Dina Sabry Abdelfattah and Rania Sayed and Mostafa-Hedeab, Gomaa and Ali, Hanan HM} } @article {193042, title = {Mesenchymal stem cells transplantation attenuates experimentally induced brain injury after neonatal hypoxia by different two routes of administrations }, journal = {Biocell}, volume = {43}, year = {2019}, pages = {21-28}, author = {Ebrahim, Nesrine and Eman Ehsan and Eman Abdel Ghany and Dina Sabry and Ashraf Shamaa} } @article {193035, title = {rs2267531, a promoter SNP within glypican-3 gene in the X chromosome, is associated with hepatocellular carcinoma in Egyptians.}, journal = {Scientific reports}, volume = {9}, year = {2019}, pages = {6868}, abstract = {

Hepatocellular carcinoma (HCC) is a major health concern in Egypt owing to the high prevalence of hepatitis C virus (HCV) infection. HCC incidence is characterized by obvious male predominance, yet the molecular mechanisms behind this gender bias are still unidentified. Functional variations in X-linked genes have more impact on males than females. Glypican-3 (GPC3) gene, located in the Xq26 region, has lately emerged as being potentially implicated in hepatocellular carcinogenesis. The current study was designed to examine the association of -784 G/C single nucleotide polymorphism (SNP) in GPC3 promoter region (rs2267531) with HCC susceptibility in male and female Egyptian HCV patients. Our results revealed a significant association between GPC3 and HCC risk in both males and females, evidenced by higher C allele and CC/C genotype frequencies in HCC patients when compared to controls. However, no such association was found when comparing HCV patients to controls. Moreover, GPC3 gene and protein expression levels were significantly higher in CC/C than in GG/G genotype carriers in males and females. The CC/C genotype exhibited a significant shorter overall survival than GG/G genotype in HCC patients. In conclusion, GPC3 rs2267531 on the X chromosome is significantly associated with HCC, but not with HCV infection, in the Egyptian population.

}, author = {Motawi, Tarek Mohamed Kamal and Sadik, Nermin Abdel Hamid and Dina Sabry and Shahin, Nancy Nabil and Fahim, Sally Atef} } @article {193037, title = {Serum L-carnitine and vitamin D levels may be low among oral sildenafil citrate non-responders.}, journal = {International journal of impotence research}, volume = {31}, year = {2019}, pages = {85-91}, abstract = {

This cross-sectional comparative study aimed to compare serum L-carnitine and 25(OH)D levels between men with ED non-responding for oral sildenafil citrate and healthy volunteers. Overall, 192 men, recruited from two University Hospitals, were allocated into two equal groups of matched age; healthy potent men and men with ED non-responders for oral sildenafil citrate. Oral sildenafil citrate non-responders self-reported inadequate erectile responses after four attempts using 100 mg with the manufacturer{\textquoteright}s guidelines relative to meals, associated medications, and sexual stimulation/arousal. Exclusion criteria were: diabetes, cardiovascular disorders, beta blockers treatment, morbid obesity, thyroid disorders, post-radical prostatectomy, and hepatic/renal failure. All participants were subjected to; history taking, clinical examination, validated IIEF-5 questionnaire, estimation of serum L-carnitine by calorimetric method and serum 25(OH)D by ELISA method. Compared with potent controls, ED men non-responders for oral sildenafil citrate showed significant decreases in the mean serum L-carnitine level (16.8 {\textpm} 3.6 uM/L versus 66.3 {\textpm} 11.9 uM/L, P = 0.001), the mean serum 25(OH)D level (21.2 {\textpm} 7.1 ng/ml versus 54.6 {\textpm} 7.9 ng/mL, P = 0.001) and IIEF-5 score (7.8 {\textpm} 2.6 versus 23.9 {\textpm} 1.3). Serum L-carnitine showed significant positive correlation with IIEF-5 scores (r = 0.873, P = 001), serum 25(OH)D (r = 0.796, P = 0.001) and significant negative correlation with the age (r = -0.515, P = 0.001). Serum 25(OH)D showed significant positive correlation with IIEF-5 scores (r = 0.855, P = 0.001) and significant negative correlation with the age (r = -0.223, P = 0.005). It is concluded that normal homeostasis of serum L-carnitine and 25(OH)D play a role in male sexual health being significantly decreased in ED non-responding for oral sildenafil citrate.

}, author = {Taymour Mostafa and Laila A. Rashed and Sabry, Dina A and Osman, Ihab and Nabil, Nashaat and Kareem, Firas and Mostafa, Ingi A} } @article {168145, title = {Genetic Variants in nicotinamide-N-methyltransferase (NNMT) gene are related to the stage of non-alcoholic fatty liver disease diagnosed by controlled attenuation parameter (CAP)-fibroscan.}, journal = {Journal of gastrointestinal and liver diseases : JGLD}, volume = {27}, year = {2018}, month = {2018 Sep}, pages = {265-272}, abstract = {

BACKGROUND AND AIMS: Various genetic polymorphisms play a key-role in the pathogenesis of NAFLD and progression to NASH with fibrosis to cirrhosis. We aimed to study the association between single-nucleotide polymorphisms (SNPs) in NNMT gene, namely rs694539 and the development of different stages of NAFLD diagnosed by controlled attenuation parameter (CAP) of FibroScan Echosens{\textregistered}.

METHODS: Transient elastography (FibroScan{\textregistered}) with controlled attenuation parameter (CAP) measurement was performed in 81 NAFLD patients (35 of them with liver biopsy) and 80 non-NAFLD controls. The accuracy of CAP and FibroScan for the detection and quantification of hepatic steatosis/fibrosis, respectively, was assessed based on liver biopsy aspect. Genetic variants of NNMT gene rs694539 were analyzed using a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).

RESULTS: According to BMI (kg/m2), among the patients, 17 (21\%) were overweight, 56 (69.1\%) obese, and 8 (9.9\%) morbidly obese. CAP and FibroScan diagnosed steatosis/fibrosis correlated significantly with liver biopsy. There was a significant association between polymorphisms of rs694539-NNMT gene and NAFLD presence and stages. The mutant type (AA-genotype) was found in 33\% NAFLD patients versus 1.2\% controls (P\<0.001), whereas the wild type (GG-genotype) was present in 21\% versus 63.8\% controls (P\<0.001). Moreover, the AA-genotype significantly correlated with the steatosis degree by CAP but not the fibrosis degree by FibroScan. Multivariate regression analysis of all the independent risk factors showed non-significant correlations with the degree of steatosis on CAP. However, by using a stepwise approach, waist circumference showed significance as an independent predictor of NAFLD.

CONCLUSIONS: Polymorphisms in rs694539-NNMT gene (mutant AA-genotype) could be a genetic risk factor for developing NAFLD and NASH (indicating susceptibility for progression and complications). Individuals with wild type (GG-genotype) are at less risk of NAFLD development. CAP and FibroScan efficiently diagnosed steatosis and fibrosis.

}, issn = {1842-1121}, doi = {10.15403/jgld.2014.1121.273.wsh}, author = {Hasan, Eman M and Abd Al Aziz, Rasha A and Dina Sabry and Darweesh, Samar K. and Badary, Hedy A and Elsharkawy, Aisha and Abouelkhair, Mahmood M and Ayman Yosry} } @article {168151, title = {Role of miRNA-210, miRNA-21 and miRNA-126 as diagnostic biomarkers in colorectal carcinoma: impact of HIF-1α-VEGF signaling pathway.}, journal = {Molecular and cellular biochemistry}, year = {2018}, month = {2018 Oct 24}, abstract = {

Colorectal cancer (CRC) is a major cause of death worldwide. Novel non-invasive, high diagnostic value screening test is urgently needed to improve survival rate, treatment and prognosis. Stable, small, circulating microRNA (miRNA) offers unique opportunities for the early diagnosis of several diseases. It acts as tumor oncogenes or suppressors and involve in cell death, survival, and metastasis. Communication between miRNA and carcinogenesis is critical but it still not clear and needs further investigation. The aim of our study is to evaluate the role of miR-210, miR-21, miR-126, as non-invasive diagnostic biomarkers for screening, early detection of CRC, studying their correlation with prognostic variables, and clarifying the roles of miRNAs on HIF-1α-VEGF signaling pathway. The expression of miR-210, miR-21 and miR-126 was performed using qRT-PCR in adenocarcinoma (no = 35), adenomas (no = 51), and neoplasm free controls (no = 101). Serum levels of VEGF and HIF-1α was determined by ELISA Kit. The results show that the expression of miR-210, miR-21, VEGF, HIF-1α was significantly up-regulated while that miRNA-126 was down-regulated in both adenocarcinoma and adenomas compared with controls (p \< 0.001 for each). No significant difference was noted comparing patients with adenocarcinoma and adenomas. The three miRNAs correlated with VEGF, HIF-α. The miR-210 and miR-21 associated with TNM classification and clinical staging of adenocarcinoma (p \< 0.001) and they show high diagnostic value with sensitivity and specificity 88.6\%, 90.1\% and 91.4\%, 95.0\% respectively. Our study revealed that circulating miR-210, miR-21 were up-regulated while miR-126 was down-regulated in CRC and adenomas patients, they all correlated with TNM staging and they had high diagnostic value. HIF-1α VEGF signaling pathways regulated by miRNAs played a role in colon cancer initiation. To the best of our knowledge, this is the first study of this miRNAs panel in CRC in our community. These data suggested that these biomarkers could be a potential novel, non-invasive marker for early diagnosis, screening and predicting prognosis of CRC. Understanding the molecular functions by which miRNAs affect cancer and understanding its roles in modulating the signaling output of VEGF might be fruitful in reducing the incidence and slowing the progression of this dark malignancy.

}, issn = {1573-4919}, doi = {10.1007/s11010-018-3462-1}, author = {Dina Sabry and El-Deek, Sahar E M and Maher, Moataz and El-Baz, Mona A H and El-Bader, Hala M and Eman Amer and Elham A. Hassan and Wael Fathy and El-Deek, Heba E M} } @article {168149, title = {Potential therapeutic effects of induced pluripotent stem cells on induced salivary gland cancer in experimental rats.}, journal = {Biotechnic \& histochemistry : official publication of the Biological Stain Commission}, year = {2018}, month = {2018 Oct 19}, pages = {1-8}, abstract = {

Salivary gland neoplasms exhibit complex histopathology in a variety of tumor types and treatment options depend largely on the stage of the cancer. Induced pluripotent stem cells (iPS) have been investigated for treating induced salivary gland cancer and for restoring salivary gland function. We investigated iPS treatment for salivary gland cancer both in vitro and in vivo. For our study in vitro, we re-programmed human skin fibroblasts to form iPS cells using a plasmid containing Oct4, Sox2, L-MYC and LIN28. For our study in vivo, we used 30 white male albino rats divided into the following groups of 10: group 1 (control): rats were injected with phosphate-buffered saline (PBS), group 2 induced squamous cell carcinoma (SCC): rat submandibular glands were injected with squamous carcinoma cells (SCC), group 3 (induced SCC/iPS): SCC treated rats treated with 5~{\texttimes}~106 iPS cells. Submandibular glands from rats of all groups were examined histologically and real time PCR was performed for amylase, and COX I and COX II gene expression. We confirmed that submandibular gland specimens included tumor tissue before starting treatment with iPS. iPS treated cases exhibited regeneration of salivary glands, although minor degenerative and vascularization changes remained. The acinar cells regained their proper organization, but continued to exhibit abnormal activity including hyperchromatism. iPS cells may be useful for treating salivary gland carcinomas.

}, issn = {1473-7760}, doi = {10.1080/10520295.2018.1508747}, author = {Alaa El-Din, Yasmine and Dina Sabry and Abdelrahman, Amal Hassan and Safa Fathy} } @article {168155, title = {Mesenchymal Stem Cell-Derived Exosomes Ameliorated Diabetic Nephropathy by Autophagy Induction through the mTOR Signaling Pathway.}, journal = {Cells}, volume = {7}, year = {2018}, month = {2018 Nov 22}, abstract = {

BACKGROUND: Diabetic nephropathy (DN) is a serious complication of diabetes mellitus and a common cause of end-stage renal disease. Autophagy has a defensive role against kidney damage caused by hyperglycemia. Mesenchymal stem cell (MSC)-derived exosomes are currently considered as a new promising therapy for chronic renal injury. However, the renal-protective mechanism of exosomes on DN is not completely understood. We examined the potential role of MSC-derived exosomes for enhancement of autophagy activity and their effect on DN. In our study, we used five groups of rats: control; DN; DN treated with exosomes; DN treated with 3-methyladenine (3-MA) and chloroquine (inhibitors of autophagy); and DN treated with 3-methyladenine (3-MA), chloroquine, and exosome groups. We assessed renal function, morphology, and fibrosis. Moreover, ratios of the autophagy markers mechanistic target of rapamycin (mTOR), Beclin-1, light chain-3 (LC3-II), and LC3-II/LC3-I were detected. Additionally, electron microscopy was used for detection of autophagosomes.

RESULTS: Exosomes markedly improved renal function and showed histological restoration of renal tissues, with significant increase of LC3 and Beclin-1, and significant decrease of mTOR and fibrotic marker expression in renal tissue. All previous effects were partially abolished by the autophagy inhibitors chloroquine and 3-MA.

CONCLUSION: We conclude that autophagy induction by exosomes could attenuate DN in a rat model of streptozotocin-induced diabetes mellitus.

}, issn = {2073-4409}, doi = {10.3390/cells7120226}, author = {Ebrahim, Nesrine and Ahmed, Inas A and Hussien, Noha I and Dessouky, Arigue A and Farid, Ayman Samir and Elshazly, Amal M and Ola Mostafa and Gazzar, Walaa Bayoumie El and Sorour, Safwa M and Seleem, Yasmin and Ahmed M. Hussein and Dina Sabry} } @article {168152, title = {Interplay Between Helicobacter pylori Infection, Interleukin-11, and Leukemia Inhibitory Factor in Gastric Cancer Among Egyptian Patients.}, journal = {Journal of interferon \& cytokine research : the official journal of the International Society for Interferon and Cytokine Research}, volume = {38}, year = {2018}, month = {2018 Nov}, pages = {517-525}, abstract = {

Helicobacter pylori is a ubiquitous Gram-negative bacterium, that is responsible for gastric mucosal inflammation. It is the most common risk factor for gastric cancer (GC). The current study aimed to investigate the association between interleukin-11 (IL-11) and leukemia inhibitory factor (LIF) levels among H. pylori-infected Egyptian patients with gastritis and GC. One hundred forty-seven patients with gastric lesions were endoscopically biopsied and assessed using rapid urease test and immunohistochemistry. Quantitative real-time polymerase chain reaction was done for the detection of H. pylori load in gastric biopsies and detection of LIF as well as IL-11 relative gene expression. The mean values of H. pylori load, LIF, and IL-11 were significantly elevated in GC patients compared to gastritis group (P \< 0.0001). A positive significant correlation was detected between mucosal levels of LIF, IL-11, and H. pylori load in both groups. Both LIF and IL-11 had the same pattern of expression in gastric tissues with different types of gastritis and different types and grades of gastric carcinoma. This report could clarify the molecular events associated with the immune response against H. pylori infection and H. pylori-associated pathology. Therefore, development of immunotherapy strategies against H. pylori-induced cytokines becomes inevitable.

}, issn = {1557-7465}, doi = {10.1089/jir.2018.0065}, author = {Dina Sabry and Abdelaleem, Omayma O and Hefzy, Enas M and Amal A. Ibrahim and Ahmed, Tarek I and Hassan, Essam A and Abdel-Hameed, Nehal D and Khalil, Mahmoud AF} } @article {151390, title = {Association of SIRT-1 Gene Polymorphism and Vitamin D Level in Egyptian Patients With Rheumatoid Arthritis.}, journal = {Journal of clinical medicine research}, volume = {10}, year = {2018}, month = {2018 Mar}, pages = {189-195}, abstract = {

Background: We investigated SIRT-1 genetic variant and its association with vitamin D level in Egyptian patients with rheumatoid arthritis (RA).

Methods: Seventy Egyptian subjects were enrolled in our study and divided into two groups: RA group (n = 50 patients) and healthy control group (n = 20 subjects). Five milliliter blood sample was withdrawn from each subject followed by laboratory investigation and DNA extraction for SIRT-1 gene polymorphism assessment (rs7895833 A\>G, rs7069102 C\>G and rs2273773 C\>T) and vitamin D level expression.

Results: There was statistically significant difference between rheumatoid cases and controls with regard to vitamin D level with 88\% of cases showing insufficient vitamin D versus all controls showing sufficient level. SIRT-1 different SNPs rs2273773, rs7895833and rs7069102 genotype frequencies were statistically significant in RA compared to control group (P = 0.001). There was no statistically significant difference between different genotypes of rs2273773, rs7895833 and rs7069102 with regard to vitamin D level.

Conclusion: We concluded that there is a strong association between SIRT-1 polymorphism genotyping and RA. Vitamin D level was insufficient in Egyptian patients with RA.

}, issn = {1918-3003}, doi = {10.14740/jocmr3067e}, attachments = {https://scholar.cu.edu.eg/sites/default/files/yasmine/files/sirt_1_vitamin_d_polymorphism.pdf}, author = {Dina Sabry and Kaddafy, Shereen Rashad and Abdelaziz, Ahmed Ali and Nassar, Abdelfattah Kasem and Rayan, Mohamed Moneer and Sadek, Sadek Mostafa and Abou-Elalla, Amany A} } @article {157512, title = {Human mesenchymal stem cell-derived extracellular vesicles/estrogen combined therapy safely ameliorates experimentally induced intrauterine adhesions in a female rat model.}, journal = {Stem cell research \& therapy}, volume = {9}, year = {2018}, month = {2018 Jun 28}, pages = {175}, abstract = {

BACKGROUND: Mesenchymal stem cells (MSCs) have diverse functions in regulating injury and inflammation through the secretion of extracellular vesicles (EVs).

METHODS: In this study, we investigated the systemic administration of extracellular vesicles derived from human umbilical cord mesenchymal stem cells (UCMSCs-EVs) as a therapeutic agent for intrauterine adhesions (IUAs) caused by endometrial injury. Additionally, we investigated the therapeutic impact of both UCMSCs-EVs and estrogen either separately or in combination in a rat model. The inflammation, vascularization, proliferation, and extent of fibrosis were assessed by a histopathological and immunohistochemical assessment using transforming growth factor (TGF)-β as a fibrotic marker and vascular endothelial growth factor (VEGF) as a vascular marker. Additionally, quantitative real-time polymerase chain reaction (qRT-PCR) was used to analyze the expression of tumor necrosis factor (TNF)-α, interleukin (IL)-1, IL-6 (inflammatory cytokines), CD140b (a marker of endometrial stem cells), and RUNX2 (an antifibrotic factor). Finally, Western blotting was used to evaluate collagen I and β-actin expression.

RESULTS: The therapeutic groups treated with either UCMSCs-EVs alone or combined with estrogen exhibited a significant decrease in inflammation and fibrosis (TNF-α, TGF-β, IL-1, IL-6, RUNX2, and collagen-I) as well as a significant decrease in vascularization (VEGF) compared with the untreated rats with IUAs. The most significant results were obtained in animals with IUAs that received a combined therapy of UCMSCs-EVs and estrogen.

CONCLUSIONS: We conclude that the synergistic action of human UCMSCs-EVs combined with estrogen provides a highly effective alternative regenerative agent in IUA treatment.

}, issn = {1757-6512}, doi = {10.1186/s13287-018-0924-z}, author = {Ebrahim, Nesrine and Ola Mostafa and El Dosoky, Rania Ebrahim and Ahmed, Inas A and Ahmed S. Saad and Abeer Mostafa and Dina Sabry and Ibrahim, Khalid Abdelaziz and Farid, Ayman Samir} } @article {157513, title = {The role of mesenchymal stem cells in chemotherapy-induced gonadotoxicity.}, journal = {Stem cell research \& therapy}, volume = {9}, year = {2018}, month = {2018 Jul 18}, pages = {196}, abstract = {

BACKGROUND: The therapeutic potential of bone marrow-derived mesenchymal stem cells (BM-MSCs) against cisplatin-induced nephrotoxicity has been reported, however, its efficacy in gonadotoxicity still has not been addressed. Herein, we investigated the effect of BM-MSCs in cisplatin-induced testicular toxicity and its underlying mechanism of action.

METHODS: Thirty male Sprague-Dawley rats were divided into a control group: injected with phosphate-buffered saline (PBS) intraperitoneal (ip), a cisplatin group: injected with a single dose of 7~mg/kg cisplatin ip to induce gonadotoxicity and a BM-MSCs group: received cisplatin ip followed by BM-MSCs injection 1 day after cisplatin. In testicular tissues, malondialdehyde (MDA), superoxide dismutase (SOD), and reduced glutathione (GSH) levels were assessed. Additionally, gene expressions of inducible nitric oxide synthase (iNOS), caspase-3, and p38 mitogen-activated protein kinase (MAPK) were measured. The testicular tumor necrosis factor alpha (TNF-α) protein contents and Bcl-2 associated X protein (BAX) expression were determined. Histopathology of testicular tissues was examined.

RESULTS: Cisplatin injection showed a significant decrease in GSH and SOD testicular levels besides a significant increase of MDA and TNF-α testicular levels and upregulation of testicular gene expressions of iNOS, caspase-3, and p38-MAPK in comparison to the control group. Moreover, a marked increase in BAX protein expression was observed in the cisplatin group when compared with the control one. Histopathological examination exhibited significant seminiferous tubules atrophy in cisplatin-treated rats.

CONCLUSIONS: The BM-MSCs injection significantly repaired the testicular injury and improved both biochemical and histopathological changes. The MSCs mitigated the gonadotoxicity induced by cisplatin through antioxidative, anti-inflammatory, and antiapoptotic mechanisms.

}, issn = {1757-6512}, doi = {10.1186/s13287-018-0946-6}, author = {Sherif, Iman O and Dina Sabry and Abdel-Aziz, Azza and Sarhan, Osama M} } @article {168153, title = {Adipose mesenchymal stem cells-derived exosomes attenuate retina degeneration of streptozotocin-induced diabetes in rabbits.}, journal = {Journal of circulating biomarkers}, volume = {7}, year = {2018}, month = {2018 Jan-Dec}, pages = {1849454418807827}, abstract = {

This study aimed to evaluate the effect of mesenchymal stem cells (MSCs)-derived exosomes in retina regeneration of experimentally induced diabetes mellitus (DM) in a rabbit model. Exosomes are extracellular vesicles that contain many microRNAs (micRNAs), mRNAs, and proteins from their cells of origin. DM was induced by intravenous (IV) injection of streptozotocin in rabbits. MSCs were isolated from adipose tissue of rabbits. Exosomes were extracted from MSCs by ultracentrifugation. Exosomes were injected by different routes (IV, subconjunctival (SC), and intraocular (IO)). Evaluation of the treatment was carried out by histopathological examination of retinal tissues and assessment of micRNA-222 expression level in retinal tissue by real-time polymerase chain reaction. Histologically, by 12 weeks following SC exosomal treatment, the cellular components of the retina were organized in well-defined layers, while IO exosomal injection showed well-defined retinal layers which were obviously similar to layers of the normal retina. However, the retina appeared after IV exosomal injection as irregular ganglionic layer with increased thickness. MicRNA-222 expression level was significantly reduced in diabetic controls when compared to each of healthy controls and other diabetic groups with IV, SC, and IO routes of injected exosomes (0.06 {\textpm} 0.02 vs. 0.51 {\textpm} 0.07, 0.28 {\textpm} 0.08, 0.48 {\textpm} 0.06, and 0.42 {\textpm} 0.11, respectively). We detected a significant negative correlation between serum glucose and retinal tissue micRNA-222 expression level ( = -0.749, = 0.001). We can associate the increased expression of micRNA-222 with regenerative changes of retina following administration of MSCs-derived exosomes. The study demonstrates the potency of rabbit adipose tissue-derived MSCs exosomes in retinal repair. So, exosomes are considered as novel therapeutic vectors in MSCs-based therapy through its role in shuttling of many factors including micRNA-222.

}, issn = {1849-4544}, doi = {10.1177/1849454418807827}, author = {Safwat, A and D. Sabry and Ragiae, A and Amer, E and Mahmoud, R H and Shamardan, R M} } @article {168147, title = {Role of mesenchymal stem cells exosomes derived microRNAs; miR-136, miR-494 and miR-495 in pre-eclampsia diagnosis and evaluation.}, journal = {Archives of biochemistry and biophysics}, volume = {659}, year = {2018}, month = {2018 Dec 01}, pages = {13-21}, abstract = {

BACKGROUND: Pre-eclampsia (PE) is one of the most threatening pregnancy complications. So far neither a secure, competent therapy for PE nor effective biomarkers for a premature discovery has been achieved. However, currently, the use of released microRNAs (miRNAs) as potential biomarkers and therapy targets for various diseases is the dominating area of research. The aim of our study was to identify miRNAs 136, 494 and 495 genes expression in exosomes of peripheral blood compared to umbilical cord mesenchymal stem cells (UCMSCs) conditioned media released exososomes in patients with PE, as valuable markers for PE early prediction.

METHODS: Blood samples were collected from 100 patients with PE and 100 control with normal pregnancies. Thirty fresh umbilical cord samples of women with healthy pregnancies (n = 15) and PE patients (n = 15) were retrieved during caesarean deliveries and UCMSCs were isolated from Wharton jelly. The expression of miRNAs 136, 494 and 495 in exosomes of peripheral blood and UCMSCs conditioned media was measured using quantitative real-time PCR method. Unpaired t-test, Pearson correlation test and Receiver operator characteristic (ROC) analysis were used for data analysis.

RESULTS: Our study revealed a significantly higher expression levels of miRNAs 136, 494 and 495 in exosomes of peripheral blood and matched with UCMSCs released exosomes from patients with PE compared to normal pregnancies (p = 0.000). In peripheral blood of PE, they were 6.4, 3.9 and 2.1 folds higher, respectively. ROC analysis revealed that the sensitivity and specificity values of miRNA-136 were 95\% and 100\%, respectively, with a cut-off value of 2.55. The sensitivity and specificity values of miRNA-494 were 86\% and 95\%, respectively, with a cut-off value of 0.47. The sensitivity and specificity values of miRNA-495 were 90\% and 83\%, respectively, with a cut-off value of 1.287.

CONCLUSION: Our findings suggest that exosomes derived miRNA-136, miRNA-494 and miRNA-495 could be promising circulating biomarkers in early detection of PE. Furthermore, UCMSCs released exosomes miRNA-136, miRNA-494 and miRNA-495 genes expression confirmed peripheral blood results analysis.

}, issn = {1096-0384}, doi = {10.1016/j.abb.2018.09.023}, author = {Tarek M. K. Motawi and Dina Sabry and Maurice, Nadine W and Sherine M. Rizk} } @article {168146, title = {Association of SPARC gene polymorphisms rs3210714 and rs7719521 with VEGF expression and utility of Nottingham Prognostic Index scoring in breast cancer in a sample of Egyptian women.}, journal = {Molecular biology reports}, volume = {45}, year = {2018}, month = {2018 Dec}, pages = {2313-2324}, abstract = {

Breast cancer is the most common malignancy in women. To our knowledge, there is no single study conducted on the role of secreted protein acidic and rich in cysteine (SPARC) gene polymorphism in breast cancer risk or prognosis. The present study aims to investigate the probable role of SPARC genetic polymorphisms in development of breast cancer; their correlation with immunohistochemical expression of VEGF; and their association with breast cancer prognosis in the Egyptian population. The study sample included 238 Egyptian females who were divided into two groups: breast cancer group (118 patients) and healthy control group (120 subjects). SPARC gene single nucleotide polymorphisms rs3210714 and rs7719521 were genotyped. Allelic and genotypic frequencies were determined in both groups and association with ductal breast carcinoma, clinicopathological and prognostic characters were determined. For SPARC rs3210714, a significant difference was observed in the codominant model and both A and G alleles{\textquoteright} frequencies between breast cancer patients and control group (P \< 0.001). For rs7719521, a significant difference in codominant and dominant models as well as in both A and C alleles{\textquoteright} frequencies between breast cancer and control groups (P \< 0.001) was observed. A significant relation was found between SPARC rs3210714 and rs7719521, and immunohistochemical expression of VEGF (P = 0.046 and P = 0.027, respectively). SPARC rs7719521 showed a significant association with Nottingham Prognostic Index (NPI) (P = 0.032). The present study revealed that SPARC rs3210714 and rs7719521 polymorphisms are associated with breast cancer risk and its prognosis. Therefore, these SNPs may be useful in predicting the increased risk of breast cancer.

}, issn = {1573-4978}, doi = {10.1007/s11033-018-4394-2}, author = {Bawazeer, Sultan and Dina Sabry and Mahmoud, Rania H and Elhanbuli, Hala M and Yassen, Noha N and Abdelhafez, Marwa N} } @article {157515, title = {Treatment Efficiency of Different Routes of Bone Marrow-Derived Mesenchymal Stem Cell Injection in Rat Liver Fibrosis Model.}, journal = {Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology}, volume = {48}, year = {2018}, month = {2018 Aug 16}, pages = {2161-2171}, abstract = {

BACKGROUND/AIMS: The most appropriate route for bone marrow-derived mesenchymal stem cell (BM-MSC) transplantation in the management of liver fibrosis remains controversial. This study investigated the therapeutic efficacy of intravenous and intrasplenic BM-MSC transplantation on carbon tetrachloride (CCl4)-induced rat liver fibrosis.

METHODS: Fifty rats were divided into 5 groups (n = 10 rats per group): healthy control group, CCl4 group, CCl4/ recovery group, CCl4/BM-MSC intravenous group, and CCl4/BM-MSC intrasplenic group. BM-MSCs were isolated, labeled with green fluorescent protein (GFP), and injected into fibrotic rats either intravenously or intrasplenically. Gene expression of interleukins (IL-1β and IL-6), interferon (INF)-γ, hepatic growth factor, and the hepatocyte-specific marker cytokeratin 18 was estimated by quantitative real-time reverse transcription-polymerase chain reaction. Vascular endothelial growth factor and connective tissue growth factor was detected by western blot analysis and enzyme-linked immunosorbent assay, respectively. At 2 weeks after intravenous and intrasplenic BM-MSC injections, GFP-positive cells were detected in liver tissue.

RESULTS: Both routes achieved a similar enhancement of liver function, which was confirmed by histopathological examination. The intravenous route was more effective than the intrasplenic route in reducing gene expression levels of IL-1β, IL-6, and INF-γ. However, fibrotic changes were still observed in the recovery group.

CONCLUSION: Intravenous BM-MSC injection was an efficient and appropriate route for BM-MSC transplantation for the management of liver fibrosis.

}, issn = {1421-9778}, doi = {10.1159/000492558}, author = {Idriss, Naglaa K and Sayyed, Hayam G and Osama, Amany and Dina Sabry} } @article {157514, title = {Heme oxygenase and iron status in exosomes of psoriasis patients.}, journal = {Archives of dermatological research}, year = {2018}, month = {2018 Aug 13}, abstract = {

Psoriasis is an autoimmune skin disease characterized by hyperproliferation of keratinocytes due to interplay between keratinocytes and immune cells. Iron status plays an important role in modifying the function of the immune system. Heme oxygenase (HO), heme-degrading enzyme, plays important role in protective response to oxidative cellular stress. We aimed in this study to map the iron status and HO levels and declare the role HO enzyme in iron homeostasis and immune-modulation in psoriasis. Fifty-one patients with psoriasis and 50 age- and sex-matched healthy controls were enrolled in this study. 5~mL blood sample was withdrawn from each subject. Hepcidin, iron soluble transferring receptor (sTfR), and total iron binding capacity (TIBC) were estimated using ELISA technique and, HO-1 gene level was detected using RT-PCR (reverse transcription-polymerase chain reaction). Iron levels, TIBC, and hepcidin were significantly lower in cases compared to controls. On the contrary, sTfR and HO-1 were significantly over-expressed in cases compared to controls (p \< 0.05 in all). HO-1 expression negatively correlated with PASI score and disease extent (\%) (r~=~- 0.614-, p = 0.001; r~=~- 0.807-, p = 0.001 respectively). There were no significant associations between HO-1 expression and iron, TIBC, hepcidin, sTfR levels (p \> 0.05 in all). Iron supplements for the patients with psoriasis are important to maintain haematopoiesis. The induction of HO-1 might have be a promising approach for the treatment of psoriasis through antioxidant ability, immunomodulatory role as well as its role in heme synthesis.

}, issn = {1432-069X}, doi = {10.1007/s00403-018-1852-6}, author = {El-Rifaie, Abdel-Aziz A and Dina Sabry and Doss, Reham W and Kamal, Mai A and Abd El Hassib, Dalia M} } @article {151391, title = {Comparative study of the osteogenic potential of mesenchymal stem cells derived from different sources}, journal = {J Clin Exp Dent. }, volume = {10}, year = {2018}, pages = {e7-13}, attachments = {https://scholar.cu.edu.eg/sites/default/files/yasmine/files/dentists_2018.pdf}, author = {Iman M. Aboushady and Zeinab A. Salem and Dina Sabry and Abbas Mohamed} } @article {151394, title = {Influence of Vitamin D Receptor Gene Polymorphisms on Response to Pegylated Interferon in Chronic Hepatitis B Egyptian Patients }, journal = {Reports of Biochemistry \& Molecular Biology }, volume = {6}, year = {2018}, pages = {186-196}, attachments = {https://scholar.cu.edu.eg/sites/default/files/yasmine/files/rbmb.pdf}, author = {Mostafa-Hedeab, Gomaa and Dina Sabry and Ghada Mostafa Abdelaziz and Manal Ewaiss and Nagla Adli and Wael Fathy} } @article {151392, title = {Preclinical Assessment of the Proliferation Capacity of Gingival and Periodontal Ligament Stem Cells from Diabetic Patients}, journal = {Open Access Macedonian Journal of Medical Sciences}, year = {2018}, pages = {1-6}, attachments = {https://scholar.cu.edu.eg/sites/default/files/yasmine/files/moustafa_paper.pdf}, author = {Mostafa Assem and Kamal, Samia and Dina Sabry and Nadia Soliman and Riham M. Aly} } @article {151388, title = {Combination of Obestatin and Bone Marrow Mesenchymal Stem Cells Prevents Aggravation of Endocrine Pancreatic Damage in Type II Diabetic Rats.}, journal = {International journal of stem cells}, volume = {10}, year = {2017}, month = {2017 Nov 30}, pages = {129-143}, abstract = {

One of the new promising therapies in treatment of diabetes mellitus is mesenchymal stem cells (MSCs) which have an interesting therapeutic potentiality based on their paracrine effect and transdifferentiation potentiality. Also obestatin improves the generation of functionalcells/islet-like cell clusters in vitro, suggesting implications for cell-based replacement therapy in diabetes. So the aim of this study was to evaluate the effect of combination of both MSCs and obestatin on an experimental model of type II diabetes mellitus (T2DM). Sixty male rats were divided into; group I (control group), group II (T2DM group) induced by administration of high fat diet (HFD) and injection of streptozotocin (STZ) in low dose, group III (T2DM treated with MSCs), group IV (T2DM treated with obestatin), group V (T2DM treated with MSCs and obestatin). Fasting blood glucose, C-peptide, insulin and lipid profile were measured. HOMA-IR and HOMA-were calculated. Pancreatic expression of insulin, glucagon like peptide -1 (GLP-1) and pancreatic duodenal homeobox 1 (Pdx1) mRNA levels were measured. In addition pancreatic histological changes, insulin and Bax were analyzed by immunohistochemical examination of islets of Langerhans. Diabetic rats showed significant increase in HOMA-IR, serum glucose and lipid profile levels with significant decrease in insulin, HOMA-, GLP-1 and Pdx1 levels. MSCs and obestatin caused significant improvement in all parameters with more significant improvement in combined therapy. The protective effects afforded by MSCs and obestatin may derive from improvement of the metabolic profile, antiapoptosis and by increase in pancreatic GLP-1and Pdx1 gene expression.

}, issn = {2005-3606}, doi = {10.15283/ijsc17035}, attachments = {https://scholar.cu.edu.eg/sites/default/files/yasmine/files/diabetes_and_obostatin.pdf}, author = {Hussien, Noha I and Ebrahim, Nesrine and Mohammed, Ola M and Dina Sabry} } @article {133546, title = {Cardioprotective Effects of Wharton Jelly Derived Mesenchymal Stem Cell Transplantation in a Rodent Model of Myocardial Injury.}, journal = {International journal of stem cells}, volume = {10}, year = {2017}, month = {2017 May 30}, pages = {48-59}, abstract = {

Background: Whartons jelly-derived mesenchymal stem cells are a valuable alternative source that possess multipotent properties, easy to obtain and available in large scale compared to BMMSCs. We investigated the possibility of cardiac function improvement post isoproterenol induced cardiac injury in a rat model following human WJMSCs transplantation.

Materials and Methods: MSCs were extracted and cultured from cord WJ, characterized by morphology, Immunophenotyping and differentiation to osteoblast and adipocytes. WJMSCs were labeled with PKH2 linker dye. Wistar rats were divided into control group, ISO group (injected with 2 doses of isoproterenol) to induce myocardial injury and ISO group transplanted with labelled WJMSCs. ECG, electrocardiographic patterns, cardiac marker enzymes, tracing of labeled MSCs and immunohistochemical analysis of myocardial cryosections were studied.

Results and Conclusions: WJ derived MSCs were expanded for more than 14 passages while maintaining their undifferentiated state, were positive for MSC markers and were able to differentiate into adipocyte and osteoblast. We demonstrated that intravenously administered WJMSCs were capable of homing predominently in the ischemic myocardium. Cardiac markers were positively altered in stem cell treated group compared to ISO group. ECG and ECHO changes were improved with higher survival rate. WJMSCs could differentiate into cardiac-like cells (positive for cardiac specific proteins) in vivo. WJMSCs infusion promoted cardiac protection and reduced mortality, emphasizing a promising therapeutic role for myocardial insufficiency.

}, issn = {2005-3606}, doi = {10.15283/ijsc16063}, author = {Taghrid Gaafar and Wael Attia and Shereen Mahmoud and Dina Sabry and AbdElAziz, Osama and DINA RASHEED and Hala Hamza} } @article {133550, title = {Angiotensin (1-7) ameliorates the structural and biochemical alterations of ovariectomy-induced osteoporosis in rats via activation of ACE-2/Mas receptor axis.}, journal = {Scientific reports}, volume = {7}, year = {2017}, month = {2017 May 23}, pages = {2293}, abstract = {

The local and systemic renin angiotensin system (RAS) influences the skeletal system micro-structure and metabolism. Studies suggested angiotensin 1-7 (Ang(1-7)) as the beneficial RAS molecule via Mas receptor activation. This study examines the function of Ang(1-7) in bone micro-architecture and metabolism in an ovariectomized (OVX) rodent model of osteoporosis. OVX rats showed structural and bone metabolic degeneration in parallel with suppressed expressions of the angiotensin converting enzyme-2 (ACE-2)/Ang(1-7)/Mas components. The infusion of Ang(1-7) markedly alleviated the altered bone metabolism and significantly enhanced both trabecular (metaphyseal) and cortical (metaphyseal-diaphyseal) morphometry. Urinary and bones minerals were also improved in OVX rats by Ang(1-7). The infusion of the heptapeptide enhanced ACE-2/Mas receptor expressions, while down-regulated AngII, ACE, and AngII type-1 receptor (AT1R) in OVX animals. Moreover, Ang(1-7) markedly improved osteoprotegerin (OPG) and lowered receptor activator NF-κB ligand (RANKL) expressions. The defensive properties of Ang(1-7) on bone metabolism, structure and minerals were considerably eradicated after blockage of Mas receptor with A-779. Ang(1-7)-induced up-regulated ACE-2/Ang(1-7)/Mas cascade and OPG expressions were abolished and the expressions of ACE/AngII/AT1R and RANKL were provoked by A-779. These findings shows for the first time the novel valuable therapeutic role of Ang(1-7) on bone health and metabolism through the ACE-2/Mas cascade.

}, issn = {2045-2322}, doi = {10.1038/s41598-017-02570-x}, author = {Abuohashish, Hatem M and Ahmed, Mohammed M and Dina Sabry and Mahmoud M. Khattab and Al-Rejaie, Salim S} } @article {133548, title = {ACE-2/Ang1-7/Mas cascade mediates ACE inhibitor, captopril, protective effects in estrogen-deficient osteoporotic rats.}, journal = {Biomedicine \& pharmacotherapy = Biomedecine \& pharmacotherapie}, volume = {92}, year = {2017}, month = {2017 May 19}, pages = {58-68}, abstract = {

The local role of the renin angiotensin system (RAS) was documented recently beside its conventional systemic functions. Studies showed that the effector angiotensin II (AngII) alters bone health, while inhibition of the angiotensin converting enzyme (ACE-1) preserved these effects. The newly identified Ang1-7 exerts numerous beneficial effects opposing the AngII. Thus, the current study examines the role of Ang1-7 in mediating the osteo-preservative effects of ACEI (captopril) through the G-protein coupled Mas receptor using an ovariectomized (OVX) rat model of osteoporosis. 8 weeks after the surgical procedures, captopril was administered orally (40mgkg(-1) d(-1)), while the specific Mas receptor blocker (A-779) was delivered at infusion rate of 400ngkg(-1)min(-1) for 6 weeks. Bone metabolic markers were measured in serum and urine. Minerals concentrations were quantified in serum, urine and femoral bones by inductive coupled plasma mass spectroscopy (ICP-MS). Trabecular and cortical morphometry was analyzed in the right distal femurs using micro-CT. Finally, the expressions of RAS peptides, enzymes and receptors along with the receptor activator of NF-κB ligand (RANKL) and osteoprotegerin (OPG) were determined femurs heads. OVX animals markedly showed altered bone metabolism and mineralization along with disturbed bone micro-structure. Captopril significantly restored the metabolic bone bio-markers and corrected Ca(2+) and P values in urine and bones of estrogen deficient rats. Moreover, the trabecular and cortical morphometric features were repaired by captopril in OVX groups. Captopril also improved the expressions of ACE-2, Ang1-7, Mas and OPG, while abolished OVX-induced up-regulation of ACE-1, AngII, Ang type 1 receptor (AT1R) and RANKL. Inhibition of Ang1-7 cascade by A-779 significantly eradicated captopril protective effects on bone metabolism, mineralization and micro-structure. A-779 also restored OVX effects on RANKL expression and ACE-1/AngII/AT1R cascade and down-regulated OPG expression and ACE-2/Ang1-7/Mas pathway. In line with the clinical observations of the bone-preservative properties following ACE-1 inhibition, local activation of ACE-2/Ang1-7/Mas signaling and suppressed osteoclastogenesis seem responsible for the osteo-preservative effect of captopril, which could offers a potential therapeutic value in treatment of disabling bone and skeletal muscular diseases.

}, issn = {1950-6007}, doi = {10.1016/j.biopha.2017.05.062}, author = {Abuohashish, Hatem M and Ahmed, Mohammed M and Dina Sabry and Mahmoud M. Khattab and Al-Rejaie, Salim S} } @article {133544, title = {Role of LncRNA-AF085935, IL-10 and IL-17 in Rheumatoid Arthritis Patients With Chronic Hepatitis C.}, journal = {Journal of clinical medicine research}, volume = {9}, year = {2017}, month = {2017 May}, pages = {416-425}, abstract = {

BACKGROUND: The current study aimed at testing the effect of corticosteroid therapy on serum levels of interleukin-10 (IL-10) and IL-17 as well as lncRNA-AF085935 in patients of rheumatoid arthritis (RA) associated with hepatitis C virus (HCV) and evaluating the usefulness of using these parameters to predict the therapeutic efficacy of steroids in these patients.

METHODS: Thirty healthy control subjects and 65 chronic HCV patients with RA were included in our study. Patients were subjected to clinical examination, abdominal ultrasound, and liver biopsy and received 6-methyl-prednisolone (PDN) 16 mg/day for 48 weeks. Blood samples were collected from all subjects and serum was separated to assess IL-10 and IL-17 by ELISA and HCV RNA and lncRNA-AF085935 by qRT-PCR.

RESULTS: Our study revealed that there were significant increases in serum levels of IL-10, IL-17 and lncRNA-AF085935 in RA patients associated with HCV compared with healthy control subjects. Also there were significant increases in serum levels of IL-10 and HCV RNA and a significant decrease in serum level of IL-17 in patients after corticosteroid therapy, while lncRNA-AF085935 is not significantly changed.

CONCLUSION: LncRNA-AF085935 might be a useful candidate biomarker for the early detection of RA associated with HCV, providing potential new strategies for early screening and therapy of these patients. IL-17 is a non-invasive prognostic marker to predict the efficacy of corticosteroid therapy in RA patients associated with chronic hepatitis C.

}, issn = {1918-3003}, doi = {10.14740/jocmr2896w}, author = {Dina Sabry and Azza El Amir and Mahmoud, Rania Hosny and Abdelaziz, Ahmed Ali and Wael Fathy} } @article {133545, title = {Role of LncRNA-AF085935, IL-10 and IL-17 in Rheumatoid Arthritis Patients With Chronic Hepatitis C.}, journal = {Journal of clinical medicine research}, volume = {9}, year = {2017}, month = {2017 May}, pages = {416-425}, abstract = {

BACKGROUND: The current study aimed at testing the effect of corticosteroid therapy on serum levels of interleukin-10 (IL-10) and IL-17 as well as lncRNA-AF085935 in patients of rheumatoid arthritis (RA) associated with hepatitis C virus (HCV) and evaluating the usefulness of using these parameters to predict the therapeutic efficacy of steroids in these patients.

METHODS: Thirty healthy control subjects and 65 chronic HCV patients with RA were included in our study. Patients were subjected to clinical examination, abdominal ultrasound, and liver biopsy and received 6-methyl-prednisolone (PDN) 16 mg/day for 48 weeks. Blood samples were collected from all subjects and serum was separated to assess IL-10 and IL-17 by ELISA and HCV RNA and lncRNA-AF085935 by qRT-PCR.

RESULTS: Our study revealed that there were significant increases in serum levels of IL-10, IL-17 and lncRNA-AF085935 in RA patients associated with HCV compared with healthy control subjects. Also there were significant increases in serum levels of IL-10 and HCV RNA and a significant decrease in serum level of IL-17 in patients after corticosteroid therapy, while lncRNA-AF085935 is not significantly changed.

CONCLUSION: LncRNA-AF085935 might be a useful candidate biomarker for the early detection of RA associated with HCV, providing potential new strategies for early screening and therapy of these patients. IL-17 is a non-invasive prognostic marker to predict the efficacy of corticosteroid therapy in RA patients associated with chronic hepatitis C.

}, issn = {1918-3003}, doi = {10.14740/jocmr2896w}, author = {Dina Sabry and Azza El Amir and Mahmoud, Rania Hosny and Abdelaziz, Ahmed Ali and Wael Fathy} } @article {151389, title = {XRCC1 Gene Polymorphisms and miR-21 Expression in Patients with Colorectal Carcinoma.}, journal = {The Eurasian journal of medicine}, volume = {49}, year = {2017}, month = {2017 Jun}, pages = {132-136}, abstract = {

OBJECTIVE: The objectives of this study were to evaluate the impact of two X-ray repair cross complementing 1 (XRCC1) gene polymorphisms (Arg194Trp and Arg399Gln) on the risk of development of colorectal cancer (CRC) and to assess the expression levels of microRNA-21 (miR-21) in CRC patients.

MATERIALS AND METHODS: A case-control cross sectional study was conducted on 50 CRC patients and 50 cancer-free subjects. DNA and miR-21 were extracted from whole blood samples. The expression levels of the XRCC1 polymorphisms and miR-21 were assessed by real-time PCR in all subjects of the study.

RESULTS: Genotype analysis revealed a significant association between CRC risk and both the Arg194Trp genotype (OR=11.407, 95\% CI=4.039-32.221, p\<0.001) and the Arg399Gln genotype (OR=3.778, 95\% CI= 1.6-8.919, p=0.002). The expression levels of circulating miR-21 were able to detect CRC cases significantly (p=0.022) with a sensitivity of 82\% and a specificity of 56\% (Area under the curve (AUC)=0.633) but were unable to distinguish between early and late cases (AJCC classification) (p=0.194).

CONCLUSION: The XRCC1 Arg194Trp and Arg399Gln polymorphisms both confer high susceptibility for the development of CRC. Circulating miR-21 expression levels are a potentially diagnostic non-invasive genetic marker of CRC.

}, issn = {1308-8734}, doi = {10.5152/eurasianjmed.2017.17021}, attachments = {https://scholar.cu.edu.eg/sites/default/files/yasmine/files/final_reprint_dr._naglaa_md_article_132-136.pdf}, author = {Hanan Fouad and Dina Sabry and Morsi, Heba and Shehab, Hany and Abuzaid, Naglaa F} } @article {133547, title = {Comparative study of antifibrotic activity of some magnesium-containing supplements on experimental liver toxicity. Molecular study.}, journal = {Drug and chemical toxicology}, volume = {40}, year = {2017}, month = {2017 Jan}, pages = {47-56}, abstract = {

INTRODUCTION: Liver fibrosis is the excessive accumulation of extracellular matrix (ECM) proteins including collagen that occurs in most types of chronic liver diseases. This study aimed to investigate and compare the therapeutic efficacy of different magnesium (Mg)-containing supplements (formulations A, B, and C) on carbon tetrachloride (CCl4)-induced liver fibrosis in rats.

METHODS: Liver fibrosis was induced by intraperitoneal injection of rats with CCl4 (1:1 in olive oil, 2 mL/kg, three times/week) for 4 weeks, and then rats were orally treated with different Mg-containing supplements (formulations A, B, and C) once daily for another one month. Liver fibrosis was quantified by evaluation of expressions of Collagen I, transforming growth factor β-1 (TGFβ1), platelet-derived growth factor-C (PDGF-C), nuclear factor kappa-β (NF-κβ), and measurement of hepatic collagen (hydroxyproline) level. Also, malondialdehyde (MDA), nitric oxide (NO), glutathione (GSH) level, superoxide dismutase (SOD), and glutathione-S-transferase (GST) activities were estimated.

RESULTS: CCl4 administration significantly elevated expressions of the studied genes, hepatic hydroxyproline, MDA, and NO levels and caused depletion of GSH level, decreased SOD, and GST activities when compared with those of their corresponding control, p \< 0.05. All magnesium supplements significantly inhibited expressions of the studied genes and attenuated the hepatic hydroxyproline level as compared with those of CCl4-treated group; p \< 0.05; for NF-κβ, the highest inhibition was by formulations B and C. Regarding Collagen I, TGFβ1, and hepatic hydroxyproline content, the highest inhibition was by Formulation C, and Formulation A revealed highest inhibition for PDGF-C. All magnesium supplements revealed normalization of oxidant and antioxidants parameters. Histopathological examination supports the biochemical and molecular findings.

CONCLUSION: Mg supplements were effective in the treatment of hepatic CCl4-induced fibrosis-rat model.

}, keywords = {Animals, Antioxidants, Biomarkers, Carbon Tetrachloride, Dietary Supplements, Female, Liver, Liver Cirrhosis, Experimental, Liver Function Tests, Magnesium, NF-kappa B, Nitric Oxide, Organ Size, Rats, Wistar, Transforming Growth Factor beta1}, issn = {1525-6014}, doi = {10.3109/01480545.2016.1172083}, author = {El-Tantawy, Walid Hamdy and Dina Sabry and Abd Al Haleem, Ekram Nemr} } @article {125829, title = {Aryl hydrocarbon receptor (AhR) rs2066853 gene polymorphism association with infertile oligoasthenoteratozoospermic men and seminal oxidative stress.}, journal = {Environmental science and pollution research international}, year = {2017}, month = {2017 Feb 04}, abstract = {

This study aimed to assess the association between aryl hydrocarbon receptor (AhR) rs2066853 gene polymorphism with infertile oligoasthenoteratozoospermic (OAT) men and seminal oxidative stress (OS). A total of 170 Egyptian men were allocated according to their semen analysis into fertile normozoospermic controls (n~=~50) and infertile OAT men (n~=~120). They were subjected to history taking, clinical examination, semen analysis, estimation of seminal glutathione peroxidase (GPx), and malondialdehyde (MDA). AhR rs2066853 gene polymorphism was identified in the blood by PCR-RFLP. Comparing infertile OAT men with fertile controls, AhR rs2066853 genotypes showed decreased prevalence for wild homozygous genotype GG (35.8 vs 56\%) and for heterozygous genotype GA (17.5 vs 30\%) and an increased prevalence for homozygous genotype AA (46.7 vs 14\%). Distribution of alleles of AhR rs2066853 among OAT men compared with fertile men showed decreased prevalence of G allele (44.6 vs 71\%) and an increased prevalence of A allele (55.4 vs 29\%). Seminal MDA demonstrated significant increase whereas seminal GPx demonstrated significant decrease in cases with AA and GA/AA genotypes compared to cases with GG genotype. It is concluded that there is a significant association between AhR rs2066853 genotype polymorphism with decreased sperm parameters as well as increased seminal oxidative stress in infertile OAT men.

}, issn = {1614-7499}, doi = {10.1007/s11356-017-8519-2}, author = {Taymour Mostafa and Hanan Fouad and Nabil, Nashaat and Laila Rashed and Dina Sabry and Abougabal, Khadiga and Gendy, Bolis S} } @article {151387, title = {Does vitamin C have the ability to augment the therapeutic effect of bone marrow-derived mesenchymal stem cells on spinal cord injury?}, journal = {Neural regeneration research}, volume = {12}, year = {2017}, month = {2017 Dec}, pages = {2050-2058}, abstract = {

Methylprednisolone (MP) is currently the only drug confirmed to exhibit a neuroprotective effect on acute spinal cord injury (SCI). Vitamin C (VC) is a natural water-soluble antioxidant that exerts neuroprotective effects through eliminating free radical damage to nerve cells. Bone marrow mesenchymal stem cells (BMMSCs), as multipotent stem cells, are promising candidates in SCI repair. To evaluate the therapeutic effects of MP, VC and BMMSCs on traumatic SCI, 80 adult male rats were randomly divided into seven groups: control, SCI (SCI induction by weight-drop method), MP (SCI induction, followed by administration of 30 mg/kg MP via the tail vein, once every other 6 hours, for five times), VC (SCI induction, followed by intraperitoneal administration of 100 mg/kg VC once a day, for 28 days), MP + VC (SCI induction, followed by administration of MP and VC as the former), BMMSCs (SCI induction, followed by injection of 3 {\texttimes} 10BMMSCs at the injury site), and BMMSCs + VC (SCI induction, followed by BMMSCs injection and VC administration as the former). Locomotor recovery was assessed using the Basso Mouse Scale. Injured spinal cord tissue was evaluated using hematoxylin-eosin staining and immunohistochemical staining. Expression of transforming growth factor-beta, tumor necrosis factor-alpha, and matrix metalloproteinase-2 genes was determined using real-time quantitative PCR. BMMSCs intervention better promoted recovery of nerve function of rats with SCI, mitigated nerve cell damage, and decreased expression of transforming growth factor-beta, tumor necrosis factor-alpha, and matrix metalloproteinase-2 genes than MP and/or VC. More importantly, BMMSCs in combination with VC induced more obvious improvements. These results suggest that VC can enhance the neuroprotective effects of BMMSCs against SCI.

}, issn = {1673-5374}, doi = {10.4103/1673-5374.221163}, attachments = {https://scholar.cu.edu.eg/sites/default/files/yasmine/files/neuralregenres.pdf}, author = {Salem, Nesrine and Mohamed Y Salem and Elmaghrabi, Mohammed M and Elawady, Moataz A and Elawady, Mona A and Dina Sabry and Ashraf Shamaa and Elkasapy, Abdel-Haleem H and Ibrhim, Noha and Azza El Amir} } @article {133549, title = {The ACE-2/Ang1-7/Mas cascade enhances bone structure and metabolism following angiotensin-II type 1 receptor blockade.}, journal = {European journal of pharmacology}, volume = {807}, year = {2017}, month = {2017 Apr 22}, pages = {44-55}, abstract = {

The renin angiotensin system (RAS) regulates numerous systemic functions and is expressed locally in skeletal tissues. Angiotensin1-7 (Ang1-7) is a beneficial member of the RAS, and the therapeutic effects of a large number of angiotensin receptors blockers (ARBs) are mediated by an Ang1-7-dependent cascade. This study examines whether the reported osteo-preservative effects of losartan are mediated through the angiotensin converting enzyme2 (ACE-2)/Ang1-7/Mas pathway in ovariectomized (OVX) rats. Sham and OVX animals received losartan (10mg/kg/d p.o.) for 6 weeks. A specific Mas receptor blocker (A-779) was delivered via mini-osmotic pumps during the losartan treatment period. Serum and urine bone metabolism biomarker levels were measured. Bone trabecular and cortical morphometry were quantified in distal femurs, whereas mineral contents were estimated in ashed bones, serum and urine. Finally, the expression of RAS components, the receptor activator of NF-κB ligand (RANKL) and osteoprotegerin (OPG) was determined. Losartan significantly improved the elevated bone metabolism marker levels and altered trabecular and cortical structures in OVX animals, and restored normal urinary and skeletal mineral levels. Mas receptor inhibition significantly abolished all osteo-protective effects of losartan and enhanced the deleterious effects of OVX. Losartan enhanced OVX-induced up-regulation of ACE-1, AngII, angiotensin type 1 (AT1) receptor and RANKL expression, and increased ACE-2, Ang1-7, Mas and OPG expression in OVX animals. However, A-779 significantly eradicated the effects of losartan on RAS components and RANKL/OPG expression. Thus, Ang1-7 are involved in the osteo-preservative effects of losartan via Mas receptor, which may add therapeutic value to this well-known antihypertensive agent.

}, issn = {1879-0712}, doi = {10.1016/j.ejphar.2017.04.031}, author = {Abuohashish, Hatem M and Ahmed, Mohammed M and Dina Sabry and Mahmoud M. Khattab and Al-Rejaie, Salim S} } @article {119161, title = {Molecular mechanisms underlying fibrosis and elastin destruction in childhood interstitial lung diseases.}, journal = {Pathophysiology : the official journal of the International Society for Pathophysiology}, year = {2016}, month = {2016 Sep 21}, abstract = {

OBJECTIVE: This study aimed to evaluate fibrosis and elastin destruction in childhood interstitial lung disease (chILD) patients.

METHODS: Sixty patients and twenty healthy children were recruited. On admission, evaluation of chILD severity was made using Fan chILD score. Participants provided urine and blood samples. Plasma levels of transforming growth factor (TGF)-β1, connective tissue growth factor (CCN2), soluble factor related apoptosis (sFas) and long non-coding RNAs and urinary levels of desmosine/urinary creatinine (UDes/UCr) were measured.

RESULTS: In patients, clinical findings were crackles (100.00\%), tachypnea (65.00\%), cardiomegaly (45.00\%), digital clubbing (43.30\%), cough (33.00\%), cyanosis (26.70\%), hepatomegaly (28.30\%) and wheezes (23.30\%). Categorizing of the patients with Fan chILD clinical score revealed that most patients 33.30\% scored (3, symptomatic with abnormal saturation/cyanosis during exercise) then 28.30\% scored (5, symptomatic with clinical and echocardiographic features of pulmonary hypertension), 18.30\% scored (2, symptomatic with normal room air saturations), 15.00\% scored (1, asymptomatic) and 5.00\% scored (4, symptomatic with abnormal room air saturation/cyanosis at rest). TGF-β1, CCN2, sFas, lncrRNA-2700086A05Rik relative gene expression and UDes/UCr levels were higher in patients than controls (P=0.002, P=0.001, P=0.001, P=0.001, P=0.001, respectively). In patients, significant positive correlations were found between TGF-β1 and CCN2, sFas, UDes/UCr; between CCN2 and both sFas and UDes/UCr; between UDes/UCr and sFas. Morbidity and mortality rates were 46.70\% and 10.00\%, respectively.

CONCLUSION: Markers of fibrosis (TGF-β1, sFas, CCN2) and elastin destruction (UDes/UCr) were increased in chILD especially in patients with long disease duration. So blockage of their pathways signals may offer novel therapeutic targets.

}, issn = {0928-4680}, doi = {10.1016/j.pathophys.2016.09.001}, author = {Hamed, Enas A and El-Saied, Mostafa M and Saad, Khaled and Yousef, Hazem Abu-Zeid and Amany O. Mohamed and Dina Sabry} } @article {121043, title = {Production of endothelial progenitor cells obtained from human Wharton{\textquoteright}s jelly using different culture conditions.}, journal = {Biotechnic \& histochemistry : official publication of the Biological Stain Commission}, volume = {91}, year = {2016}, month = {2016 Nov}, pages = {532-539}, abstract = {

Endothelial progenitor cells (EPC) participate in revascularization and angiogenesis. EPC can be cultured in vitro from mononuclear cells of peripheral blood, umbilical cord blood or bone marrow; they also can be transdifferentiated from mesenchymal stem cells (MSC). We isolated EPCs from Wharton{\textquoteright}s jelly (WJ) using two methods. The first method was by obtaining MSC from WJ and characterizing them by flow cytometry and their adipogenic and osteogenic differentiation, then applying endothelial growth differentiating media. The second method was by direct culture of cells derived from WJ into endothelial differentiating media. EPCs were characterized by morphology, Dil-LDL uptake/UEA-1 immunostaining and testing the expression of endothelial markers by flow cytometry and RT-PCR. We found that MSC derived from WJ differentiated into endothelial-like cells using simple culture conditions with endothelium induction agents in the medium.

}, issn = {1473-7760}, doi = {10.1080/10520295.2016.1250284}, author = {Zayed, S A and T.M. Gaafar and Samy, R M and D. Sabry and AS Nasr and Maksoud, Fa Abdel} } @article {121044, title = {Production of endothelial progenitor cells obtained from human Wharton{\textquoteright}s jelly using different culture conditions.}, journal = {Biotechnic \& histochemistry : official publication of the Biological Stain Commission}, volume = {91}, year = {2016}, month = {2016 Nov}, pages = {532-539}, abstract = {

Endothelial progenitor cells (EPC) participate in revascularization and angiogenesis. EPC can be cultured in vitro from mononuclear cells of peripheral blood, umbilical cord blood or bone marrow; they also can be transdifferentiated from mesenchymal stem cells (MSC). We isolated EPCs from Wharton{\textquoteright}s jelly (WJ) using two methods. The first method was by obtaining MSC from WJ and characterizing them by flow cytometry and their adipogenic and osteogenic differentiation, then applying endothelial growth differentiating media. The second method was by direct culture of cells derived from WJ into endothelial differentiating media. EPCs were characterized by morphology, Dil-LDL uptake/UEA-1 immunostaining and testing the expression of endothelial markers by flow cytometry and RT-PCR. We found that MSC derived from WJ differentiated into endothelial-like cells using simple culture conditions with endothelium induction agents in the medium.

}, issn = {1473-7760}, doi = {10.1080/10520295.2016.1250284}, author = {Zayed, S A and T.M. Gaafar and Samy, R M and D. Sabry and AS Nasr and Maksoud, Fa Abdel} } @article {112290, title = {Comparative study of antifibrotic activity of some magnesium-containing supplements on experimental liver toxicity. Molecular study.}, journal = {Drug and chemical toxicology}, year = {2016}, month = {2016 May 5}, pages = {1-10}, abstract = {

INTRODUCTION: Liver fibrosis is the excessive accumulation of extracellular matrix (ECM) proteins including collagen that occurs in most types of chronic liver diseases. This study aimed to investigate and compare the therapeutic efficacy of different magnesium (Mg)-containing supplements (formulations A, B, and C) on carbon tetrachloride (CCl4)-induced liver fibrosis in rats.

METHODS: Liver fibrosis was induced by intraperitoneal injection of rats with CCl4 (1:1 in olive oil, 2 mL/kg, three times/week) for 4 weeks, and then rats were orally treated with different Mg-containing supplements (formulations A, B, and C) once daily for another one month. Liver fibrosis was quantified by evaluation of expressions of Collagen I, transforming growth factor β-1 (TGFβ1), platelet-derived growth factor-C (PDGF-C), nuclear factor kappa-β (NF-κβ), and measurement of hepatic collagen (hydroxyproline) level. Also, malondialdehyde (MDA), nitric oxide (NO), glutathione (GSH) level, superoxide dismutase (SOD), and glutathione-S-transferase (GST) activities were estimated.

RESULTS: CCl4 administration significantly elevated expressions of the studied genes, hepatic hydroxyproline, MDA, and NO levels and caused depletion of GSH level, decreased SOD, and GST activities when compared with those of their corresponding control, p \< 0.05. All magnesium supplements significantly inhibited expressions of the studied genes and attenuated the hepatic hydroxyproline level as compared with those of CCl4-treated group; p \< 0.05; for NF-κβ, the highest inhibition was by formulations B and C. Regarding Collagen I, TGFβ1, and hepatic hydroxyproline content, the highest inhibition was by Formulation C, and Formulation A revealed highest inhibition for PDGF-C. All magnesium supplements revealed normalization of oxidant and antioxidants parameters. Histopathological examination supports the biochemical and molecular findings.

CONCLUSION: Mg supplements were effective in the treatment of hepatic CCl4-induced fibrosis-rat model.

}, issn = {1525-6014}, doi = {10.3109/01480545.2016.1172083}, author = {El-Tantawy, Walid Hamdy and Dina Sabry and Abd Al Haleem, Ekram Nemr} } @article {117237, title = {Comparative Evaluation for Potential Differentiation of Endothelial Progenitor Cells and Mesenchymal Stem Cells into Endothelial-Like Cells.}, journal = {International journal of stem cells}, volume = {9}, year = {2016}, month = {2016 May 30}, pages = {44-52}, abstract = {

Understanding the mechanisms of vascular remodeling could lead to more effective treatments for ischemic conditions. We aimed to compare between the abilities of both human Wharton jelly derived mesenchymal stem cells (hMSCs) and human cord blood endothelial progenitor cells (hEPCs) and CD34$^{+}$ to induce angiogenesis in vitro. hMSCs, hEPCs, and CD34$^{+}$ were isolated from human umbilical cord blood using microbead (MiniMacs). The cells characterization was assessed by flow cytometry following culture and real-time PCR for vascular endothelial growth factor receptor 2 (VEGFR2) and von Willebrand factor (vWF) to prove stem cells differentiation. The study revealed successful isolation of hEPCs, CD34$^{+}$, and hMSCs. The hMSCs were identified by gaining CD29$^{+}$ and CD44$^{+}$ using FACS analysis. The hEPCs were identified by having CD133$^{+}$, CD34$^{+}$, and KDR. The potential ability of hEPCs and CD34$^{+}$ to differentiate into endothelial-like cells was more than hMSCs. This finding was assessed morphologically in culture and by higher significant VEGFR2 and vWF genes expression (p\<0.05) in differentiated hEPCs and CD34$^{+}$ compared to differentiated hMSCs. hEPCs and CD34$^{+}$ differentiation into endothelial-like cells were much better than that of hMSCs.

}, issn = {2005-3606}, doi = {10.15283/ijsc.2016.9.1.44}, author = {Dina Sabry and Olfat Noh, and Mai Samir} } @article {108991, title = {Hepatitis C Viral Kinetic Changes in a Retrospective Cohort Study of Chronic Hepatitis C Virus Egyptian Patients on Pegylated Interferon and Ribavirin Therapy.}, journal = {Journal of interferon \& cytokine research : the official journal of the International Society for Interferon and Cytokine Research}, volume = {36}, year = {2016}, month = {2016 Mar}, pages = {149-58}, abstract = {

The aim of this study was to determine the relative importance of the kinetics of antiviral response compared to baseline host and virological factors for predicting treatment outcome. A retrospective analysis of 285 chronic hepatitis C virus (HCV) patients, encompassing genotypes 4 treated with peginterferon alpha-2a and ribavirin, was performed. Baseline characteristics were compared across HCV genotypes and pretreatment factors associated with rapid virological response (RVR) were identified. The relative significance of RVR compared to other baseline factors for predicting sustained virological response was analyzed by multiple logistic regression analysis. Ninety-seven percent of the patients harbored HCV genotype 4a patients. The positive predictive value (PPV) of RVR for end-of-treatment response (ETR) was 88\% and of early virological response (EVR) was 85\%, which means that achievement of both RVR and EVR is a good positive predictive factor of response. The negative predictive value (NPV) of RVR for ETR was low and equals 26.77\%, which means that approximately two-thirds of patients were able to achieve ETR despite not experiencing RVR, which means RVR is a bad negative predictive factor of response. The NPV of EVR for ETR was high and equals 90\%, which means that only 10\% of patients were able to achieve an ETR despite not experiencing EVR, which explains that EVR is a very good negative predictive factor of response. In univariate logistic regression analysis, which included the following: female gender, alanine aminotransferase, aspartate transaminase, α-fetoprotein, baseline HCV-RNA levels, grade of activity, stage of fibrosis, and positive HCV-RNA, by polymerase chain reaction at week 4, none of the previous factors was a significant independent factor of failure of response to treatment. The current study demonstrated that a viremia at week 4 has a good PPV, but it has a very low NPV. The NPV of EVR was more robust for ETR (90\%). EVR is regarded as a robust indicator of treatment outcome, and a 12-week stopping rule for patients is strongly evident.

}, issn = {1557-7465}, doi = {10.1089/jir.2015.0099}, author = {Esmat, Gamal Eldeen and Wafa Al Akel and Rasha Ahmed Abdel Aziz and Al Sayed Taha, Ahmed and Dina Sabry and Laila A. Rashed and Mostafa, Aya and El Kazaz, Amany Y and Sahar H. Ahmed} } @article {108686, title = {Therapeutic efficacy of amniotic membrane stem cells and adipose tissue stem cells in rats with chemically induced ovarian failure.}, journal = {Journal of advanced research}, volume = {7}, year = {2016}, month = {2016 Mar}, pages = {233-41}, abstract = {

The present study was conducted to compare between the therapeutic efficacies of human amniotic membrane-derived stem cells (hAM-MSCs) vs. adipose tissue derived stem cells (AD-MSCs) in cyclophosphamide (CTX)-induced ovarian failure in rats. Forty-eight adult female rats were included in the study; 10 rats were used as control group. Thirty-eight rats were injected with CTX to induce ovarian failure and divided into four groups: ovarian failure (IOF) (IOF group), IOF~+~phosphate buffer saline (PBS group), IOF~+~hAM-MSCs group and IOF~+~AD-MSCs group. Serum levels of FSH and estradiol (E2) were assessed. Histopathological examination of the ovarian tissues was performed and quantitative gene expressions of Oct-4, Stra8 and integrin beta-1 genes were conducted by quantitative real time PCR. Results showed that IOF and IOF~+~PBS rat groups exhibited decreased ovarian follicles, increased interstitial fibrosis with significant decrease of serum E2, significant increase serum FSH level and significant down-regulation of Stra8 and integrin beta-1. In hAM-MSCs and AD-MSCs rat groups, there were increased follicles and corpora with evident the presence of oocytes, significant increase in serum E2, significant decrease in serum FSH levels (in hAM-MSCs treated group only) and significant up-regulation of the three studied genes with higher levels in hAM-MSCs treated rats group when compared to AD-MSCs treated rats group. In Conclusion, administration of either hAM-derived MSCs or AD-MSCs exerts a significant therapeutic efficacy in chemotherapy induced ovarian insult in rats. hAM-MSCs exert higher therapeutic efficacy as compared to AD-MSCs.

}, issn = {2090-1232}, doi = {10.1016/j.jare.2015.05.002}, author = {Hanan Fouad and Dina Sabry and Khaled Elsetohy and Naglaa Fathy} } @article {117235, title = {SIRT-1expression is associated with expression of NANOG in patients with colorectal adenocarcinoma.}, journal = {Cancer biomarkers : section A of Disease markers}, volume = {17}, year = {2016}, month = {2016 Jun 24}, pages = {155-163}, abstract = {

AIMS: The study aimed to investigate the quantitative expression of NANOG, p38 α , NCF2, ELF and TGF-β genes in patients with colorectal adenocarcinoma, adenoma and normal colonic tissue and their correlation with SIRT-1 protein level expression.

METHOD: This study enrolled one hundred sixty seven patients; group A: 87 patients with colonoscopic findings of no adenoma or adenocarcinoma and group B: 80 patients with colorectal mass. Consecutive colonoscopic examinations were conducted, and tissue samples were taken from the colonic lesions/masses. Total RNA was isolated and mRNA expression level of NANOG, mitogen activated p38α , Neutrophil Cytosol Factor 2 (NCF2), Embryonic Liver Fodrin (ELF) and Transforming Growth Factor Beta (TGF-β) genes were quantified by qRT-PCR. Sirt-1 protein expression level was assessed by quantitative western blot.

RESULTS: There were significantly high level of mRNA transcripts expression of the genes studied in patients with adenocarcinoma and adenoma compared with normal tissue (P value \< 0.01), NANOG, NCF2, ELF and TGF-β at a cut of \> 0.314, \> 0.392, 0.349 and 0.333 respectively showed sensitivity (96.5\%, 98.8\%, 95.3\%, 98.8\%) and specificity of (95.3\%, 92.6\%, 89.5\%, 93.8\%) respectively in diagnosing colonic adenocarcinoma. Sirt-1 protein level was significantly highly expressed in colorectal adenocarcinoma compared to normal and adenoma colonic tissue and positively correlated with NANOG.

CONCLUSION: Over expression of NANOG, p38α , NCF2, ELF and TGF-β genes in both cases of adenocarcinoma and adenoma could have a diagnostic value. SIRT-1 and NANOG are high correlated biological markers for diagnosis and prognosis follow up in patients with adenocarcinoma.

}, issn = {1875-8592}, doi = {10.3233/CBM-160626}, author = {Osama, Amany and Dina Sabry and Hassany, Sahar M and Abdelmoneim, Soha Saoud and Sabry, Abeer} } @article {112166, title = {Molecular assessment of Vitamin D receptor polymorphism as a valid predictor to the response of Interferon/Ribavirin based therapy in Egyptian patients with Chronic Hepatitis C.}, journal = {Journal of digestive diseases}, year = {2016}, month = {2016 Apr 29}, abstract = {

BACKGROUND/AIM: Egypt has the highest prevalence rate of Hepatitis C virus (HCV) infection around the globe, where, chronic hepatits C (CHC) is considered a major health problem. The standard treatment of CHC is combination therapy of pegylated interferon and ribavirin. Successful treatment and sustained virological response (SVR) are only achieved in 30\% of patients. Major adverse effects and high cost of the treatment makes predicting the treatment output is an important approach. The aim of this study to find an association between Vitamin D concentration and vitamin D receptor (VDR) polymorphisms with achieving SVR.

METHODS: This is a case control study in which; 250 patients recruited and were divided into 3 groups (100 CHC patients who achieved SVR, 100 CHC patients who did not achieve SVR, and 50 apparently healthy individuals as control). Blood samples were collected to measure vitamin D concentration and 4 VDR polymorphisms (FokI, ApaI, TaqI, and BsmI) were detected using RFLP-PCR.

RESULTS: Non responders were found to have significantly low vitamin D concentration compared to responders and control groups. Concerning VDR polymorphisms, both FokI and TaqI polymorphisms were associated to successful treatment.

CONCLUSION: Vitamin D concentration, FokI, and TaqI may be considered as one of the predictors for the response of CHC patients to combination of pegylated interferon and ribvirin therapy. This article is protected by copyright. All rights reserved.

}, issn = {1751-2980}, doi = {10.1111/1751-2980.12353}, author = {Abdelsalam, Ahmed and Laila Rashed and Salman, Tarek and Hammad, Lamiaa and Dina Sabry} } @article {120457, title = {Comparison of the therapeutic effectiveness of human CD34(+) and rat bone marrow mesenchymal stem cells on improvement of experimental liver fibrosis in Wistar rats.}, journal = {International journal of physiology, pathophysiology and pharmacology}, volume = {8}, year = {2016}, month = {2016}, pages = {128-139}, abstract = {

BACKGROUND AND OBJECTIVE: Human umbilical cord blood (UCB) cells and bone marrow mesenchymal stem cells (BM-MSCs) have numerous advantages as grafts for cell transplantation. We hypothesized differing impacts of human UCB cells and rat BM-MSCs on reversal of hepatic injury and revival of liver function in carbon tetrachloride (CCl4)-induced liver fibrosis.

METHODS: Forty rats were divided into 4 groups; control group, CCl4 group, CCl4/CD34(+) group and CCl4/BM-MSCs group. Blood samples were driven from rats at 4, 8 and 12 weeks to measure serum concentration of albumin and alanine aminotransferase (ALT). Quantitative expression of collagen Iα, TGF-β, α-SMA, albumin, MMP-2, MMP-9 and TNF-α were assessed by polymerase chain reaction. Histopathological examination of the liver tissue was performed. GFP labeled cells were detected in groups injected with stem cells.

RESULTS: Regarding liver function, CD34(+) were more efficient than BM-MSCs in elevating albumin (P\<0.05) and reducing ALT (P\<0.05) concentrations. Concerning gene expression, CD34(+) were more effective than BM-MSCs in reducing gene expressions of collagen Iα (P\<0.01), TGF-β1 (P\<0.01) and α-SMA (P\<0.01). Both CD34(+) and BM-MSCs have the same efficacy in reducing TNF-α (P\<0.001 and P\<0.01, respectively). Furthermore, CD34(+) were more valuable than BM-MSCs in increasing gene expression of albumin (P\<0.05) and MMP-9 (P\<0.01).

CONCLUSION: Taken together; human UCB CD34(+) stem cells were more efficient in improvement of experimental liver injury than BM-MSCs. This study highlighted an important role of human UCB CD34(+) stem cells in liver fibrosis therapy.

}, author = {Sayyed, Hayam G and Osama, Amany and Idriss, Naglaa K and Dina Sabry and Abdelrhim, Azza S and Bakry, Rania} } @article {117236, title = {Umbilical cord mesenchymal stem cells derived extracellular vesicles can safely ameliorate the progression of chronic kidney diseases.}, journal = {Biomaterials research}, volume = {20}, year = {2016}, month = {2016}, pages = {21}, abstract = {

BACKGROUND: Bio-products from stem/progenitor cells, such as extracellular vesicles, are likely a new promising approach for reprogramming resident cells in both acute and chronic kidney disease. Forty CKD patients stage III and IV (eGFR 15-60~mg/ml) have been divided into two groups; twenty patients as treatment group "A" and twenty patients as a matching placebo group "B". Two doses of MSC-derived extracellular vesicles had been administered to patients of group "A". Blood urea, serum creatinine, urinary albumin creatinine ratio (UACR) and estimated glomerular filtration rate (eGFR) have been used to assess kidney functions and TNF-α, TGF-β1 and IL-10 have been used to assess the amelioration of the inflammatory immune activity.

RESULTS: Participants in group A exhibited significant improvement of eGFR, serum creatinine level, blood urea and UACR. Patients of the treatment group "A" also exhibited significant increase in plasma levels of TGF-β1, and IL-10 and significant decrease in plasma levels of TNF-α. Participants of the control group B did not show significant improvement in any of the previously mentioned parameters at any time point of the study period.

CONCLUSION: Administration of cell-free cord-blood mesenchymal stem cells derived extracellular vesicles (CF-CB-MSCs-EVs) is safe and can ameliorate the inflammatory immune reaction and improve the overall kidney function in grade III-IV CKD patients.

}, issn = {2055-7124}, doi = {10.1186/s40824-016-0068-0}, author = {Nassar, Wael and Mervat El-Ansary and Dina Sabry and Mostafa, Mostafa A and Tarek Fayad and Kotb, Esam and Temraz, Mahmoud and Saad, Abdel-Naser and Essa, Wael and Adel, Heba} } @article {111796, title = {Braf, Kras and Helicobacter pylori epigenetic changes-associated chronic gastritis in Egyptian patients with and without gastric cancer.}, journal = {World journal of microbiology and biotechnology}, volume = {32}, year = {2016}, pages = {92}, author = {Sabry D and Ahmed R and Abdalla S and Fathy W, and Eldemery A, and Elamir A} } @article {108074, title = {Circulating Endothelial Cells and Endothelial Function Predict Major Adverse Cardiac Events and Early Adverse Left Ventricular Remodeling in Patients With ST-Segment Elevation Myocardial Infarction}, journal = {Journal of interventional cardiology}, volume = {29}, year = {2016}, pages = {89-98}, author = {Hamid, Magdy Abdel and Sameh W. G. Bakhoum and Yasser Sharaf, and Dina Sabry and Ahmed T El-Gengehe, and Ahmed Abdel-Latif} } @article {108071, title = {Comparative evaluation of PLGA nanoparticle delivery system for 5-fluorouracil and curcumin on squamous cell carcinoma}, journal = {Archives of oral biology}, volume = {64}, year = {2016}, pages = {1-10}, author = {Shaimaa M Masloub, and Mohamed H Elmalahy, and Dina Sabry and Wael S Mohamed, and Sahar H. Ahmed} } @article {108077, title = {Influence of delta virus infection on the virologic status in Egyptian patients with chronic Hepatitis B virus genotype D}, journal = {J Med Virol.}, volume = {88}, year = {2016}, pages = {837-42}, author = {Fouad, Rabab and Mahmoud Abdo and Hadeel Gamal El deen and Dina Sabry and Mira Atef, and Ahmed, Rasha and Zayed, Naglaa} } @article {108170, title = {Therapeutic efficacy of amniotic membrane stem cells and adipose tissue stem cells in rats with chemically induced ovarian failure}, journal = {Journal of Advanced Research}, year = {2016}, author = {Hanan Fouad and Dina Sabry and Khaled Elsetohy and Naglaa Fathy} } @article {108072, title = {Therapeutic efficacy of differentiated versus undifferentiated mesenchymal stem cells in experimental type I diabetes in rat}, journal = {Biochemistry and Biophysics Reports}, volume = {5}, year = {2016}, pages = {468-475}, author = {M.A. Wassef and H. Fouad and D. Sabry and N Afifi, and Abbas, AM and W Mostafa and S. H. Ahmed} } @article {108078, title = { Dina Sabry [HTML] from nih.gov Low-density lipoprotein receptor genetic polymorphism in chronic hepatitis C virus Egyptian patients affects treatment response}, journal = {World journal of gastroenterology: WJG}, volume = {21}, year = {2015}, pages = {11141}, author = {Mazen Naga and Amin, Mona and Dina Algendy and Ahmed Elbadry and May Fawzi and Ayman Foda and Serag Esmat and Dina Sabry and Laila Rashed and Samia Gabal and Manal Kamal} } @article {108080, title = {CLINICAL SIGNIFICANCE OF SERUM N-TERMINAL PRO C-TYPE NATRIURETIC PEPTIDE IN HEPATITIS C-RELATED CHRONIC LIVER DISEASES.}, journal = {Journal of the Egyptian Society of Parasitology}, volume = {45}, year = {2015}, pages = {219-26}, author = {NADIA A. ABDELKADER and MONA ZAKI and WESSAM E. SAAD and Gehan Hamdy and Dina Sabry} } @article {108076, title = {Correlation between Vitamin D Receptor and Monocyte Chemotactic Protein-1 Polymorphisms and Spontaneous Bacterial Peritonitis in Decompensated Liver Disease}, journal = {Journal of Gastroenterology and Hepatology Research}, volume = {4}, year = {2015}, pages = {1815-1820}, author = {Nadia Abdelaaty Abdelkader, and Dina Sabry and Mohamed Al-Ghussein, and Hany Mansour Dabbous, and Enas Hassan Allam} } @article {108070, title = {Effect of vitamin D therapy on interleukin-6, visfatin, and hyaluronic acid levels in chronic hepatitis C Egyptian patients}, journal = {Therapeutics and clinical risk management}, volume = {11}, year = {2015}, pages = {279}, author = {Dina Sabry and Mohamed A. S. Al-Ghussein and Gehan Hamdy and Abul-Fotouh, Amr and Tarek Motawi and El Kazaz, Amany Y and Ahmed Eldemery and Marwa Shaker} } @article {108068, title = {Efficacy of a novel water-soluble curcumin derivative versus sildenafil citrate in mediating erectile function}, journal = {International journal of impotence research}, volume = {27}, year = {2015}, pages = {9-15}, author = {A.M.S Zaahkouk and Abdel Aziz, M.T and A.M. Rezq and Atta, HM and H.H. Fouad and Ahmed, H. H. and D. Sabry and M.H. Yehia} } @article {108067, title = {Endothelial nitric oxide synthase gene polymorphism relationship with semen parameters and oxidative stress in infertile oligoasthenoteratozoospermic men}, journal = {Urology}, volume = {85}, year = {2015}, pages = {1058-1061}, author = {Taymour Mostafa and Laila A. Rashed and Nabil, Nashaat and Hanan Fouad and Dina Sabry and Dalia M El-Saied} } @article {107410, title = {Endothelial progenitor cells regenerate infracted myocardium with neovascularisation development.}, journal = {Journal of Advanced Research}, volume = {6}, year = {2015}, pages = {133{\textendash}144}, abstract = {

We achieved possibility of isolation, characterization human umbilical cord blood endothelial progenitor cells (EPCs), examination potency of EPCs to form new blood vessels and differentiation into cardiomyoctes in canines with acute myocardial infarction (AMI). EPCs were separated and cultured from umbilical cord blood. Their phenotypes were confirmed by uptake of double stains dioctadecyl tetramethylindocarbocyanine-labeled acetylated LDL and FITC-labeled Ulex europaeus agglutinin 1 (DILDL-UEA-1). EPCs of cord blood were counted. Human VEGFR-2 and eNOS from the cultured EPCs were assessed by qPCR. Human EPCs was transplanted intramyocardially in canines with AMI. ECG and cardiac enzymes (CK-MB and Troponin I) were measured to assess severity of cellular damage. Histopathology was done to assess neovascularisation. Immunostaining was done to detect EPCs transdifferentiation into cardiomyocytes in peri-infarct cardiac tissue. qPCR for human genes (hVEGFR-2, and eNOS) was done to assess homing and angiogenic function of transplanted EPCs. Cultured human cord blood exhibited an increased number of EPCs and significant high expression of hVEGFR-2 and eNOS genes in the culture cells. Histopathology showed increased neovascularization and immunostaining showed presence of EPCs newly differentiated into cardiomyocyte-like cells. Our findings suggested that hEPCs can mediate angiogenesis and differentiate into cardiomyoctes in canines with AMI.

Abbreviations
CTO, chronic total occlusion; CAG, coronary angiography; AMI, acute myocardial infarction; DILDL-FITC labeled UEA-11, 1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine-labeled acetylated LDL (DiLDL,) and FITC-labeled Ulex europaeus agglutinin-1; MVD, multivessel disease; CFU, colony forming unit

}, author = {M.T. Abd El Aziza and E.A. Abd El Nabia and M. Abd El Hamidc and D. Sabrya and H.M. Attaa and L.A. Raheda and A. Shamaae and S. Mahfouzf} } @article {108066, title = {Impact of vitamin D supplementation on sustained virological response in chronic hepatitis C genotype 4 patients treated by pegylated interferon/ribavirin}, journal = {Journal of Interferon \& Cytokine Research}, volume = {35}, year = {2015}, pages = {49-54}, author = {Gamal Esmat and Maissa El Raziky and Elsharkawy, Aisha and Dina Sabry and Mohamed Hassany, A and Amal Ahmed and Assem, Noha and El Kassas, Mohamad and Wahid D} } @article {108075, title = {Maternal chronic oral infection with periodontitis and pericoronitis as a possible risk factor for preeclampsia in Egyptian pregnant women (microbiological and serological study)}, journal = {Future Dental Journal}, volume = {1}, year = {2015}, pages = {23-32}, author = {Lobna A Aly, and Hala El- Menoufy and Rehab Tarek Elsharkawy, and Mona Z Zaghloul, and Dina Sabry} } @article {108069, title = {Molecular signalling of a novel curcumin derivative versus Tadalafil in erectile dysfunction}, journal = {Andrologia}, volume = {47}, year = {2015}, pages = {616-625}, author = {Abdel Aziz, M.T and A.M. Rezq and Atta, HM and H. Fouad and AM Zaahkouk, and Ahmed, H. H. and D. Sabry and HM Yehia} } @article {108169, title = {Potential proliferative effect of lipopolysaccharide preconditioning on human umbilical cord blood-derived endothelial progenitor cells}, journal = {African Journal of Biotechnology}, volume = {14}, year = {2015}, pages = {1167-1173}, author = {SH Ahmad, and Dina Sabry and Olfat Noh, and Mai Samir} } @article {108168, title = {Potential therapeutic effect of amniotic stem cell transplantation on ovarian function and folliculogenesis in rats with induced ovarian failure}, journal = {Journal of Evidence-Based Women{\textquoteright}s Health Journal Society}, volume = {5}, year = {2015}, pages = {99-110}, author = {Hanan Fouad and Dina Sabry and Khaled Elsetohy and Naglaa Fathy and DINA OMAR} } @article {108171, title = {SP746ASSESSMENT OF HEPATIC FIBROSIS BY FIBROSCAN IN EGYPTIAN CHRONIC HEMODIALYSIS PATIENTS WITH HCV GENOTYPE 4}, journal = {Nephrology Dialysis Transplantation}, volume = {30}, year = {2015}, pages = {iii624-iii624}, author = {Bahaa Moustafa Zayed, and Mohamd Megdy and Dina Sabry} } @article {108178, title = {ARE RENALASE RS2576178 AND RS10887800 POLYMORPHISMS ASSOCIATED WITH PREGNANCY INDUCED HYPERTENSION?}, journal = {WORLD JOURNAL OF PHARMACY AND PHARMACEUTICAL SCIENCES}, volume = {3}, year = {2014}, pages = {177-192}, author = {Khaled A. Elsetohy and Mohamed A. S. Al-Ghussein and Dina Sabry and Adel M. Nada and Ashraf A. Eldaly and Amr H. Wahba} } @article {108191, title = {Bridging defects in chronic spinal cord injury using peripheral nerve grafts combined with a chitosan-laminin scaffold and enhancing regeneration through them by co-transplantation with bone-marrow-derived mesenchymal stem cells: case series of 14 patient}, journal = {J Spinal Cord Med.}, volume = {37}, year = {2014}, pages = {54-71}, author = {Amr SM and Gouda A and Koptan WT and Galal AA and Abdel-Fattah DS and Rashed LA and Atta HM and Abdel-Aziz MT.} } @proceedings {108172, title = {Circulating EPCs predicts the occurrence of major adverse cardiac events and early adverse remodeling in patients with STEMI}, journal = {EUROPEAN HEART JOURNAL}, year = {2014}, pages = {651-651}, publisher = {35}, address = {OXFORD UNIV PRESS}, author = {M. Abdelhamid and Y Sharaf, and Bakhoum, S and D. Sabry and Talaat, A.} } @proceedings {108174, title = {Comparative study between the effects of human CD34+ and rat bone marrow mesenchymal stem cells on amelioration of CCL4 induced liver fibrosis}, journal = {FEBS JOURNAL}, year = {2014}, pages = {222-222}, publisher = {281}, address = {France WILEY-BLACKWELL}, author = {D. Sabry and Osama, A. and N Idriss, and Magdy, M.} } @proceedings {108175, title = {Effect of Hemochromatosis gene mutation on HCV response to treatment in the Egyptian population}, journal = {HEPATOLOGY}, year = {2014}, pages = {931A-932A}, publisher = {60}, address = {WILEY-BLACKWELL}, author = {Mazen I Naga, and Amin, Mona A and Dina A Algendy, and ahmed i elbadry and Mai M Fawzi, and Ayman R Foda, and Serag M Esmat, and Dina Sabry and Laila A. Rashed and Manal Kamal} } @article {108186, title = {Effects of a novel curcumin derivative on insulin synthesis and secretion in streptozotocin-treated rat pancreatic islets in vitro}, journal = {Chinese medicine}, volume = {9}, year = {2014}, pages = {1-12}, author = {Aziz, Mohammed Talaat Abdel and El-Asmar, Mohammed Farid and Rezq, Ameen Mahmoud and Wassef, Mohammed Abdel Aziz and Hanan Fouad and Nagwa Kamal Roshy and Ahmed, Hanan Hosni and Laila Ahmed Rashed and Dina Sabry and Fatma Mohammed Taha and Hassouna, Amira} } @article {108187, title = {Efficiency of systemic versus intralesional bone marrow-derived stem cells in regeneration of oral mucosa after induction of formocresol induced ulcers in dogs}, journal = {Dental research journal}, volume = {11}, year = {2014}, pages = {212}, author = {Lobna A Aly, and Hala El- Menoufy and Hesham S Sadeq, and Alyaa Ragae and Dina Sabry} } @article {108179, title = {IN VITRO AND IN VIVO EVALUATION ACTIVITIES OF EACH MESENCHYMAL STEM CELLS, DOXORUBICIN AND LPS OR COMBINED ON BREAST CARCINOMA}, journal = {WORLD JOURNAL OF PHARMACY AND PHARMACEUTICAL SCIENCES}, volume = {3}, year = {2014}, pages = {157-176}, author = {Abdel Aziz, Mohamed T and Dina Sabry and Mohamed A. S. Al-Ghussein and Abeer M A Soliman and Ayman A Hassan} } @proceedings {108177, title = {Influence of Delta Virus Infection on the Clinical Spectrum, Serologic and Virologic Status in Chronic Hepatitis B Virus Genotype D}, journal = {HEPATOLOGY}, year = {2014}, pages = {971A-972A}, publisher = {60}, address = { WILEY-BLACKWELL}, author = {Omar, Rabab F. and Ahmed, Rasha and Dina Sabry and Mahmoud Abdel Alim, and Mira Atef, and Zayed, Naglaa} } @article {108183, title = {Molecular detection of monocyte chemotactic protein-1 polymorphism in spontaneous bacterial peritonitis patients}, journal = {World journal of gastroenterology: WJG}, volume = {20}, year = {2014}, pages = {11793-11799}, author = {Maysa Kamal Salama and Dina Sabry and MA Al-Ghussein, and Ahmed, Rasha and Allah, Sayed Abd and Taha, Fatma Mohamed and Wael Fathy and Wadie, Miriam Safwat and Nabih, Mona and Abul-Fotouh, Amr and Tarneem Darwish} } @proceedings {108182, title = {P1101 THE VITAMIN D RECEPTOR FOKI GENE POLYMORPHISM IS ASSOCIATED WITH POOR RESPONSE TO TREATMENT IN CHRONIC HEPATITIS C GENOTYPE 4}, journal = {HEPATOLOGY}, year = {2014}, pages = {S445}, publisher = {60}, address = {WILEY-BLACKWELL}, author = {Shehab, H and D. Sabry and M Mokhtar, and Mabrouk, M. and Elakel, W. and Tawfik, M and Y Korriem, and Esmat, G.} } @article {108184, title = {Pilot study on molecular quantitation and sequencing of endometrial cytokines gene expression and their effect on the outcome of in vitro fertilization (IVF) cycle}, journal = {Journal of Advanced Research}, volume = {5}, year = {2014}, pages = {595-600}, author = {D. Sabry and O. Nouh and Marzouk, S and A. Hassouna} } @article {108185, title = {Potential therapeutic utility of mesenchymal stem cells in inflammatory bowel disease in mice}, journal = {International immunopharmacology}, volume = {22}, year = {2014}, pages = {515-521}, author = {Ahmed G Abdel Salam, and Hazem M Ata, and Tarek M Salman and Laila A. Rashed and Dina Sabry and Mona F. Schaalan} } @proceedings {108176, title = {The Role Of Genetic Polymorphism Of LDL Receptor In Egyptian Population Infected With Chronic HCV, Regarding Response To Treatment}, journal = {HEPATOLOGY}, year = {2014}, pages = {922A-922A}, publisher = {60}, address = { WILEY-BLACKWELL}, author = {Mazen I Naga, and Amin, Mona A and Dina A Algendy, and ahmed i elbadry and Mai M Fawzi, and Ayman R Foda, and Serag M Esmat, and Dina Sabry and Laila A. Rashed and Manal Kamal and Samia M. Gabal} } @proceedings {108173, title = {Statin treatment increases the circulating endothelial progenitor cells and improve endothelial function in patients with coronary artery diseases}, journal = {EUROPEAN HEART JOURNAL}, year = {2014}, pages = {1154-1154}, publisher = {35}, address = {OXFORD UNIV PRESS}, author = {M. Abdelhamid and D. Sabry and D Gharib, and Abdel Gawad, M} } @article {108180, title = {Vitamin D and IL28B genotyping as predictors for antiviral therapy: a retrospective study in Egyptian HCV genotype 4a}, journal = {Tropical Journal of Pharmaceutical Research}, volume = {13}, year = {2014}, pages = {1725-1732}, author = {Nadia Abdelaaty Abdelkader, and Soha Saoud Abdelmoniem, and Dina Sabry and Amin Mohamad Abdelbaky, and Maram M Mahdy, and Zaky, Eman and Wessam Elsayed} } @article {108189, title = {Comparative diagnostic study of biomarkers using FibroMax{\texttrademark} and pathology for prediction of liver steatosis in patients with chronic hepatitis C virus infection: an Egyptian study.}, journal = {Int J Gen Med}, volume = {12}, year = {2013}, pages = {127-34}, author = {Fouad A and Sabry D and Ahmed R and Kamal M and Allah SA, and Marzouk S, and Amin M and Abd El Aziz R, and El Badri A, and Khattab H and Helmy D.} } @article {108188, title = {Dendritic cell co-stimulatory and co-inhibitory markers in chronic HCV: an Egyptian study.}, journal = {World journal of gastroenterology: WJG}, volume = {19}, year = {2013}, pages = {7711-8}, author = {Fouad H and Raziky MS, and Aziz RA, and Sabry D and Aziz GM, and Ewais M, and Sayed AR.} } @article {36420, title = {Effect of mesenchymal stem cells and a novel curcumin derivative on Notch1 signaling in hepatoma cell line.}, journal = {Biomed Res Int}, year = {2013}, abstract = {

This study was conducted to evaluate the effect of mesenchymal stem cells (MSCs) and a novel curcumin derivative (NCD) on HepG2 cells (hepatoma cell line) and to investigate their effect on Notch1 signaling pathway target genes. HepG2 cells were divided into HepG2 control group, HepG2 cells treated with MSC conditioned medium (MSCs CM), HepG2 cells treated with a NCD, HepG2 cells treated with MSCs CM and NCD, and HepG2 cells treated with MSCs CM (CM of MSCs pretreated with a NCD). Expression of Notch1, Hes1, VEGF, and cyclin D1 was assessed by real-time, reverse transcription-polymerase chain reaction (RT-PCR) in HepG2 cells. In addition, HepG2 proliferation assay was performed in all groups. Notch1 and its target genes (Hes1 and cyclin D1) were downregulated in all treated groups with more suppressive effect in the groups treated with both MSCs and NCD. Also, treated HepG2 cells showed significant decrease in cell proliferation rate. These data suggest that modulation of Notch1 signaling pathway by MSCs and/or NCD can be considered as a therapeutic target in HCC.

}, attachments = {https://scholar.cu.edu.eg/sites/default/files/yasmine/files/bmri2013-129629.pdf}, author = {Abdel Aziz MT1 and Khaled HM and El Hindawi A and Roshdy NK and Rashed LA, Sabry D, Hassouna AA, Taha F, Ali WI. and Hassouna AA, and Taha F and Ali WI.} } @article {36425, title = {Pilot study on molecular quantitation and sequencing of endometrial cytokines gene expression and their effect on the outcome of in vitro fertilization (IVF) cycle}, year = {2013}, attachments = {https://scholar.cu.edu.eg/sites/default/files/yasmine/files/jare_251_1.pdf}, author = {D. Sabrya and O. Nouhb and S. Marzouka and A. Hassounaa} } @article {36419, title = {Short-term evaluation of autologous transplantation of bone marrow-derived mesenchymal stem cells in patients with cirrhosis: Egyptian study}, journal = {Clin Transplant.}, volume = {27}, year = {2013}, pages = {607-12}, abstract = {

Stem cell-based therapy has received attention as a possible alternative to organ transplantation. The aim of this study was to assess the safety and efficacy of autologous transplantation of bone marrow (BM)-derived stromal cells in post-HCV liver cirrhosis patients.
METHODOLOGY:
10 {\texttimes} 10(6) of isolated human bone marrow (HBM)-stromal cells in 10 mL normal saline were injected in the spleen of 20 patients with end-stage liver cirrhosis guided by the ultrasonography, and then patients were followed up on monthly basis for six months.
RESULTS:
A statistically significant decrease was detected in the total bilirubin, aspartate transaminase (AST), alanine transaminase (ALT) (p-value\<0.01), prothrombin time (PT), and international normalized ratio (INR) levels (p-value\<0.05), while a statistically significant increase in the albumin and PC (p-value\<0.05) after follow-up.
CONCLUSION:
This study suggested the safety, feasibility, and efficacy of the intrasplenic injection of autologous BM stromal cells in improving liver function in Egyptian patients with cirrhosis.
{\textcopyright} 2013 John Wiley \& Sons A/S.

}, author = {Amin MA1 and Sabry D and Rashed LA and Aref WM and el-Ghobary MA and Farhan MS and Fouad HA and Youssef YA.} } @article {108190, title = {Signaling mechanisms of a water soluble curcumin derivative in experimental type 1 diabetes with cardiomyopathy.}, journal = {Diabetol Metab Syndr.}, volume = {12}, year = {2013}, pages = {13}, author = {Aziz MT and El Ibrashy IN, and Mikhailidis DP and Rezq AM and Wassef MA and Fouad HH and Ahmed HH and Sabry DA, and Shawky HM, and Hussein RE.} } @article {roshdy2011efficacy, title = {Efficacy of mesenchymal stem cells in suppression of hepatocarcinorigenesis in rats: possible role of Wnt signaling}, year = {2011}, note = {n/a}, abstract = {n/a}, author = {Nagwa K Roshdy and Laila A. Rashed and Dina Sabry and Amira A Hassouna and Fatma M Taha} } @article {mokbel2011homing, title = {Homing and reparative effect of intra-articular injection of autologus mesenchymal stem cells in osteoarthritic animal model}, journal = {BMC musculoskeletal disorders}, volume = {12}, number = {1}, year = {2011}, note = {n/a}, pages = {259}, publisher = {BioMed Central Ltd}, abstract = {n/a}, author = {Abir N. Mokbel and El Tookhy, Omar S and Ashraf A. Shamaa and Laila A. Rashed and Dina Sabry and Abeer M. Elsayed} } @article {haleem2010clinical, title = {The Clinical Use of Human Culture{\textendash}Expanded Autologous Bone Marrow Mesenchymal Stem Cells Transplanted on Platelet-Rich Fibrin Glue in the Treatment of Articular Cartilage Defects A Pilot Study and Preliminary Results}, journal = {Cartilage}, volume = {1}, number = {4}, year = {2010}, note = {n/a}, pages = {253{\textendash}261}, publisher = {SAGE Publications}, abstract = {n/a}, author = {Haleem, Amgad M and El Singergy, Abdel Aziz and Dina Sabry and Hazem M Atta and Laila A. Rashed and Chu, Constance R and El Shewy, Mohammed T and Azzam, Akram and Mohammed T. Abdel Aziz} } @article {abdel2010effect, title = {Effect of mesenchymal stem cell penile transplantation on erectile signaling of aged rats}, journal = {Andrologia}, volume = {42}, number = {3}, year = {2010}, note = {n/a}, pages = {187{\textendash}192}, publisher = {Wiley Online Library}, abstract = {n/a}, author = {Abdel Aziz, M.T and S. El-Haggar and Mostafa, T and Atta, H. and H. Fouad and S. Mahfouz and L. Rashed and D. Sabry and A. Senbel and G.A. Ali} } @article {abdel2010heme, title = {Heme oxygenase enzyme activity in seminal plasma of oligoasthenoteratozoospermic males with varicocele}, journal = {Andrologia}, volume = {42}, number = {4}, year = {2010}, note = {n/a}, pages = {236{\textendash}241}, publisher = {Wiley Online Library}, abstract = {n/a}, author = {Abdel Aziz, M.T and Mostafa, T and Atta, H. and O. Kamal and M. Kamel and H. Hosni and L. Rashed and D. Sabry and F. Waheed} } @article {el2010role, title = {The role of bone marrow-derived mesenchymal stem cells in treating formocresol induced oral ulcers in dogs}, journal = {Journal of oral pathology \& medicine}, volume = {39}, number = {4}, year = {2010}, note = {n/a}, pages = {281{\textendash}289}, publisher = {Wiley Online Library}, abstract = {n/a}, author = {El-Menoufy, H and Aly, LAA and Aziz, MTA and Atta, HM and N.K. Roshdy and L.A. Rashed and D. Sabry} } @article {aziz2009effects, title = {Effects of Losartan, HO-1 Inducers or HO-1 Inhibitors on Erectile Signaling in Diabetic Rats}, journal = {The journal of sexual medicine}, volume = {6}, number = {12}, year = {2009}, note = {n/a}, pages = {3254{\textendash}3264}, publisher = {Wiley Online Library}, abstract = {n/a}, author = {Abd El Aziz and Talaat, Mohamed and Asmer, El and Farid, Mohamed and Taymour Mostafa and Hazem Atta and Soheir Mahfouz and Hanan Fouad and Laila Rashed and Dina Sabry and others} } @article {abdel2009putative, title = {Putative role of carbon monoxide signaling pathway in penile erectile function}, journal = {The journal of sexual medicine}, volume = {6}, number = {1}, year = {2009}, note = {n/a}, pages = {49{\textendash}60}, publisher = {Wiley Online Library}, abstract = {n/a}, author = {Abdel Aziz, Mohamed T and Taymour Mostafa and Hazem Atta and Wassef, Mohamed A and Hanan H Fouad and Laila A. Rashed and Dina Sabry} } @article {abdel2008effect, title = {Effect of hemin and carbon monoxide releasing molecule (CORM-3) on cGMP in rat penile tissue.}, journal = {The journal of sexual medicine}, volume = {5}, number = {2}, year = {2008}, note = {n/a}, pages = {336{\textendash}343}, abstract = {n/a}, author = {Abdel, Aziz MT and El-Asmar, M Farid and Taymour Mostafa and Hazem Atta and H.H. Fouad and N.K. Roshdy and L.A. Rashed and E.A. Obaia and D.A. Sabry and Abdel, Aziz AT and others} } @article {aziz2008role, title = {The role of PDE5 inhibitors in heme oxygenase{\textendash}cGMP relationship in rat cavernous tissues}, journal = {The Journal of Sexual Medicine}, volume = {5}, number = {7}, year = {2008}, note = {n/a}, pages = {1636{\textendash}1645}, publisher = {Wiley Online Library}, abstract = {n/a}, author = {Aziz, M and Taymour Mostafa and Hazem Atta and Laila Rashed and Marzouk, Samar A and Obaia, Eman M and Dina Sabry and Amira A Hassouna and Amal M. El-Shehaby and Aziz, Ahmed T Abdel} } @article {aziz2007assessment, title = {Assessment of heme oxygenase-1 (HO-1) activity in the cavernous tissues of sildenafil citrate-treated rats}, journal = {Asian journal of andrology}, volume = {9}, number = {3}, year = {2007}, note = {n/a}, pages = {377{\textendash}381}, publisher = {Wiley Online Library}, abstract = {n/a}, author = {Abd El Aziz and Talaat, M and Al-Asmar, M Farid and Taymour Mostafa and Hazem Atta and Laila Rashed and Dina Sabry and Ashour, Shedeed and Abdel Aziz, Ahmed T} } @article {abdel2007oral, title = {Oral phosphodiesterase-5 inhibitors: effect of heme oxygenase inhibition on cGMP signalling in rat cavernous tissue}, journal = {Andrologia}, volume = {39}, number = {2}, year = {2007}, note = {n/a}, pages = {66{\textendash}70}, publisher = {Wiley Online Library}, abstract = {n/a}, author = {Abdel Aziz, M.T and Mostafa, T and Atta, H. and L. Rashed and S.A. Marzouk and E.M. Obaia and D. Sabry and A.A. Hassouna and A.M. El-Shehaby and Abdel Aziz, AT} } @article {aziz2007original, title = {ORIGINAL RESEARCH{\textemdash}BASIC SCIENCE: Heme Oxygenase vs. Nitric Oxide Synthase in Signaling Mediating Sildenafil Citrate Action}, journal = {The journal of sexual medicine}, volume = {4}, number = {4ii}, year = {2007}, note = {n/a}, pages = {1098{\textendash}1107}, publisher = {Wiley Online Library}, abstract = {n/a}, author = {Abd El Aziz and Talaat, M and El-Asmer, Mohamed Farid and Taymour Mostafa and Mostafa, Samia and Hazem Atta and Abdel Aziz Wassef, M and Hanan Fouad and Laila Rashed and Dina Sabry and others} } @article {abdel2007therapeutic, title = {Therapeutic potential of bone marrow-derived mesenchymal stem cells on experimental liver fibrosis}, journal = {Clinical biochemistry}, volume = {40}, number = {12}, year = {2007}, note = {n/a}, pages = {893{\textendash}899}, publisher = {Elsevier}, abstract = {n/a}, author = {Abdel Aziz, M.T and Atta, HM and S. Mahfouz and H.H. Fouad and N.K. Roshdy and Ahmed, H. H. and L.A. Rashed and D. Sabry and A.A. Hassouna and Hasan, NM} } @article {aziz2005effects, title = {Effects of nitric oxide synthase and heme oxygenase inducers and inhibitors on molecular signaling of erectile function}, journal = {Journal of Clinical Biochemistry and Nutrition}, volume = {37}, number = {3}, year = {2005}, note = {n/a}, pages = {103{\textendash}111}, publisher = {日本酸化ストレス学会 JCBN 事務局}, abstract = {n/a}, author = {Aziz, Mohammed Talaat Abdel and El-Asmar, Mohammed Farid and Taymour Mostafa and Hazem Atta and Wassef, Mohammed Abdel Aziz and Fouad, Hanan Hassan and Nagwa Kamal Roshdy and Laila Ahmed Rashed and Dina Sabry} }