Kadry, W., M. Eldeftar, Y. Nassar, A. Abou-El-Fetouh, and M. M. Hakam, "Clinical, volumetric and densitometric evaluation of tissue engineered constructs for secondary alveolar cleft reconstruction: A randomized clinical trial.", Journal of cranio-maxillo-facial surgery : official publication of the European Association for Cranio-Maxillo-Facial Surgery, vol. 49, issue 12, pp. 1141-1150, 2021. Abstract

The objective of this study was to assess the efficacy of tissue engineered (TE) constructs compared to the standard autogenous anterior iliac crest (AIC) bone graft in secondary maxillary alveolar cleft reconstruction. This clinical trial was registered on (clinical trials.gov); the trial registry number is: NCT03563495. Patients with unilateral alveolar clefts were allocated on the basis of simple randomization. Selected patients were randomly divided into two equal groups: the AIC group (control) received autogenous particulate bone graft from anterior iliac crest, whereas the TE group received tissue engineered construct. Assessment included the newly formed bone volume and density at the grafted cleft sites, which were measured immediately, 6 and 12 months postoperatively on axial computed tomograms. The study population included 10 patients; each group comprised 5 patients. There was no statistically significant difference between the 2 groups regarding the mean volume of the newly formed bone (p = 0.91 at 6 months, p = 0.994 at 1 year) and the mean density of the newly formed bone (p = 0.364 at 6 months, p = 0.073 at 1 year). However, there was a significant increase of TE graft density from 6 months to 1 year (p = 0.048). Within the limitations of the study it seems that the tissue engineered construct (collagen/osteogenically differentiated bone marrow-derived mesenchymal stem cells) might be an alternative to autogenous bone for unilateral secondary alveolar cleftgrafting.

W.K.Hafez, S.A.Seif, H. Shawky, and M.M.Hakam, "Platelet rich fibrin as a membrane for coverage of immediate implants: Case-series study on eight patients", Tanta Dental Journal, vol. 12, issue 3, pp. 203-210, 2015. AbstractWebsite


To evaluate the efficacy of platelet rich fibrin as a membrane for coverage of immediate implants in the maxillary anterior region.

Patients and methods

Twelve implants were inserted into eight adult patients indicated for extraction & immediate implant insertion in one or more of the upper anterior teeth. A venous blood sample of 10 cc was obtained for each single implant. After implant placement, the peri-implant defect was filled with a mixture of autogenous bone (collected from the chin) and fibrin. A platelet rich fibrin membrane was used to cover the implant site. Clinical & radiographic evaluation was performed immediately, 3 and 6 months postoperatively to evaluate soft tissue healing & crestal bone stability.


After 6 months, the marginal bone was stable in 83% of cases. Clinically, good soft tissue coverage was obtained. Radiographically, bone height showed a statistically significant decrease in the distal side while there was no statistically significant decrease at the mesial side. The change in defect depth was limited to 2 mm.


Platelet rich fibrin provided good soft tissue coverage over the immediate implants and it enhanced bone stability. The technique was easy to perform with good esthetic results.

Hafez", "W. K. M., "M. M. Hakam", "M. M.Dahaba", and "S. A. Seif", "Evaluation of marginal bone stability around immediate implants", Cairo Dental Journal, vol. 28, pp. 1487:1494, 2012.