Shiba, H. F., M. K. El-Ghamrawy, I. A. E. - M. Shaheen, R. A. E. - G. Ali, and S. M. Mousa,
"Glutathione S-Transferase Gene Polymorphisms (GSTM1, GSTT1, and GSTP1) in Egyptian Pediatric Patients with Sickle Cell Disease",
Pediatric and Developmental Pathology, vol. 17, issue 4: The Society for Pediatric Pathology and the Paediatric Pathology Society, pp. 265 - 270, 2014.
AbstractABSTRACT Sickle cell disease (SCD) complications are associated with oxidative stress. Glutathione S-transferases (GSTs) are a group of enzymes that protect against oxidative stress. The aims of this study was to evaluate the prevalence of GSTM1, GSTT1, and GSTP1 gene polymorphisms among homozygous sickle cell anemia patients and to investigate the possible association between the presence of these polymorphisms and SCD severity and complications. Genotyping the polymorphisms in GSTT1 and GSTM1 genes was performed using the multiplex polymerase chain reaction (PCR) method. The GSTP1 ILe105Val polymorphism was determined using PCR?restriction fragment length polymorphism. GSTM1 null genotype was significantly associated with increased risk of severe vaso-occlusive crises (VOC) (odds ratio ?=? 1.52, 95% confidence interval ?=? 0.42?5.56, P ?=? 0.005). We found no significant association between GST genotypes and frequency of sickle cell?related pain, transfusion frequency, disease severity, or hydroxyurea treatment. GSTM1 gene polymorphism may be associated with risk of severe VOC among Egyptian SCD patients.ABSTRACT Sickle cell disease (SCD) complications are associated with oxidative stress. Glutathione S-transferases (GSTs) are a group of enzymes that protect against oxidative stress. The aims of this study was to evaluate the prevalence of GSTM1, GSTT1, and GSTP1 gene polymorphisms among homozygous sickle cell anemia patients and to investigate the possible association between the presence of these polymorphisms and SCD severity and complications. Genotyping the polymorphisms in GSTT1 and GSTM1 genes was performed using the multiplex polymerase chain reaction (PCR) method. The GSTP1 ILe105Val polymorphism was determined using PCR?restriction fragment length polymorphism. GSTM1 null genotype was significantly associated with increased risk of severe vaso-occlusive crises (VOC) (odds ratio ?=? 1.52, 95% confidence interval ?=? 0.42?5.56, P ?=? 0.005). We found no significant association between GST genotypes and frequency of sickle cell?related pain, transfusion frequency, disease severity, or hydroxyurea treatment. GSTM1 gene polymorphism may be associated with risk of severe VOC among Egyptian SCD patients.
Mousa, S., S. Mostafa, I. Shahenn, and E. Elnoshokaty,
"Detection of trisomy 4 and 10 in Egyptian pediatric patients with acute lymphoblastic leukemia",
Clin Lab., vol. 60, issue 4, pp. 609-14, 2014.
El-Mahgoub, E. R., E. Ahmed, R. A. - E. A. Afifi, M. - A. Kamal, and S. M. Mousa,
"Mesenchymal Stem Cells from Pediatric Patients with Aplastic Anemia: Isolation, Characterization, Adipogenic, and Osteogenic Differentiation",
Fetal & Pediatric Pathology, vol. 33, no. 1, pp. 9-15, 2014.
Abstract
Nasef, A., M. Ibrahim, N. Riad, and S. Mousa,
"Plasma annexin A5, anti-annexin A5 antibodies and annexin A5 polymorphism in Egyptian female patients with systemic lupus erythematosus and antiphospholipid syndrome.",
Clin Lab., vol. 60, issue 1, pp. 133-7, 2014.
El-Din, M. - A. K., M. S. Farhan, R. I. E. Shiha, R. M. H. El-Kaffas, and S. M. Mousa,
"Frequency of CYP2C9 and VKORC1 Gene Polymorphisms and Their Influence on Warfarin Dose in Egyptian Pediatric Patients",
Pediatric Drugs, vol. 16, no. 4: Springer International Publishing, pp. 337-341, 2014.
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