Bio

  • Master thesis: "STUDIES ON MASTITIS IN DAIRY HERDS"

Name: SOLIMAN MOHAMMED SOLIMAN HASEN
Nationality: Egyptian
Birth date: 7/2/1983, Cairo
Degree: Master, 2009
Specification: Infectious Diseases
Supervisors :
Prof. Abou-Zeid Abd El-Megid Abou-Zeid
Prof. Magdy Mahmoud El-Sayed
Prof. Mahmoud Esam Hatem
Thesis Title :
"STUDIES ON MASTITIS IN DAIRY CATTLE"
Abstract
Total number of 415 QMS of subclinical, clinical mastitis and healthy control milk samples was collected from cows. The percentage of subclinical mastitis was 73.71 %, while that of clinical mastitis was 7.5 %. The relationship between the SCC and the bacteriological examination of the milk samples was studied as well as the correlation between CMT and the bacteriological examination of milk samples. Sensitivity and specificity of both CMT and SCC were studied. Sensitivity of SCC was 90.1% while specificity was 72.7 % and so its accuracy was 89.6 %. Sensitivity of CMT was 87.1% while specificity was 70%. Sensitivity of the isolated bacteria were studied and found that the most effective antibiotic(s) are Amoxicillin / Clavulanic acid, Gentamicin, Chloramphenicol and Cloxacillin were the most effective antibiotics. Regarding the influence of infectious agents associated with mastitis E.coli was the most prevalent isolated species followed by CNS, K. pneumoniae, S. aureus and Pseudomonas.

 

  • PhD Thesis: "MOLECULAR-BIOLOGICAL STUDIES ON BOVINE MASTITIS"

Name: Soliman Mohammed Soliman Hasan
Nationality: Egyptian
Birth date: 7/2/1983, Cairo
Degree: PhD, 2014
Specification: Infectious Diseases
Supervisors:
Prof. Amal Abd El-Aziz Hasan El-Molla
Prof. Samia Abd El-Hamid Ahmed
Prof. Walid Sayed Ahmed Awad
Thesis Title:
"MOLECULAR-BIOLOGICAL STUDIES ON BOVINE MASTITIS"
Abstract
This study was carried out on 1281 Friesian dairy cows in private dairy farms and small stock holders located in Port-Said, Qaliubiya, Damietta, Monofia and those located on the Cairo-Alexandria Desert Road to study the mastitis problem with special reference was done to Staphylococcal mastitis. Clinical examination, CMT and SCC, PCR and gene typing in addition to sensitivity against some antibiotics were done in this study. 91 cows with 335 QMS were examined bacteriologically. They recorded that 122 QMS were positive while 231 QMS were negative for bacteriological examination. 97 strains of S. aureus, 17 strains of CNS and eight strains of streptococci isolated from positive QMS. PCR was used for direct detection of S. aureus in raw milk as a rapid diagnostic tool. Genotyping of virulence factors encoding genes of S. aureus was done. Coagulase gene, Thermonuclease gene, gene segment encoding the immunoglobulin G binding region and the Staphylococcal enterotoxin genes group (SEs) {A, C, D, G, H, I, J and L} categorizing the pathogenic virulent S. aureus into enterotoxigenic and non- enterotoxigenic. Sensitivity test against some antibiotics was done for both S. aureus and CNS. S. aureus was sensitive to Gentamycin and Amoxicillin /Calvulanic acid, while CNS strains were sensitive to Amoxicillin /Calvulanic acid and Cloxacillin.
Keywords: Mastitis, Cows, S. aureus, PCR, Genotypes, Virulence genes, Milk.