Sherif Mehanny, PhD

Tour the lab, bench assignments - Sterile technique, microplate reader/spectrophotometer software demo - Aseptic technique, cell mass determination, and microscopy - Isolation of plasmid DNA, restriction digests, agarose gel electrophoresis - Polymerase chain reaction (PCR) - Bacterial transformation using electroporation and calcium heat shock - Batch growth and nutrient uptake 
Fermentation - Cell lysis and protein purification by ammonium sulfate fractionation - Protein purification by gel filtration chromatography - Protein purification by affinity chromatography -Enzyme kinetics