Ahmed, S., M. A. Kassem, and S. Sayed, "Bilosomes as promising nanovesicular carriers for improved transdermal delivery: construction, in vitro optimization, ex vivo permeation and in vivo evaluation", International Journal of Nanomedicine, vol. 15: Dove Press, pp. 9783, 2020. Abstract
Ahmed, S., M. A. Kassem, and S. Sayed, Co-polymer mixed micelles enhanced transdermal transport of Lornoxicam: in vitro characterization, and in vivo assessment of anti-inflammatory effect and antinociceptive activity, , vol. 62, pp. 102365, 2021. AbstractWebsite

The objective of this study was to develop mixed micelles of Lornoxicam, using a Pluronic® mixture of L121 and P123. The technique used for the preparation was thin-film hydration according to a Box Behnken design with three central points for each formula. The independent variables studied were Pluronic®: drug ratio, Pluronic® L121: Pluronic® P123 ratio and amount of soya-bean lecithin. The studied dependent variables were: percent entrapment efficiency (% EE), particle size (PS), poly-dispersity index (PDI), zeta potential (ZP) and in vitro LX released (after 2, 10 h). Numerical optimization was used to statistically optimize the formulae using Design-Expert® software. Choosing the best formula was in terms of maximizing % EE, ZP (as absolute value) and in vitro release, while minimizing PS and PDI. Characterization of best formula was performed by Transmission electron microscopy, Differential scanning calorimetry, Fourier transform infrared spectroscopy, stability testing, ex vivo permeation and skin deposition studies using rat skin. The in vivo pharmacodynamics activities of the optimized formula were examined on male rats and mice and related to that of the oral commercial product. The optimized formula demonstrated to be non-irritant, with considerably improved anti-inflammatory and analgesic activities. Higher in vivo skin permeation was confirmed using confocal laser scanning microscopy. In conclusion, the obtained results proved that mixed micelles could be promising method for transdermal drug delivery.

Sayed, S., M. Abdel-Moteleb, M. M. Amin, and O. M. Khowessah, "Cubogel as potential platform for glaucoma management", Drug DeliveryDrug Delivery, vol. 28, issue 1: Taylor & Francis, pp. 293 - 305, 2021. AbstractWebsite
Sayed, S., F. M. Elsharkawy, M. M. Amin, H. A. Shamsel-Din, and ahmed b ibrahim, "Brain targeting efficiency of intranasal clozapine-loaded mixed micelles following radio labeling with Technetium-99m", Drug DeliveryDrug Delivery, vol. 28, issue 1: Taylor & Francis, pp. 1524 - 1538, 2021. AbstractWebsite
Habib, B. A., S. Sayed, and G. M. Elsayed, "Enhanced transdermal delivery of ondansetron using nanovesicular systems: Fabrication, characterization, optimization and ex-vivo permeation study-Box-Cox transformation practical example.", European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences, vol. 115, pp. 352-361, 2018 Mar 30. Abstract

This study aimed to formulate suitable nanovesicles (NVs) for transdermal delivery of Ondansetron. It also illustrated a practical example for the importance of Box-Cox transformation. A 2 full factorial design was used to enable testing transfersomes, ethosomes, and transethosomes of Ondansetron simultaneously. The independent variables (IVs) studied were sodium taurocholate amount, ethanol volume in hydration medium and sonication time. The studied dependent variables (DVs) were: particle size (PS), zeta potential (ZP) and entrapment efficiency (EE). Polynomial equations were used to study the influence of IVs on each DV. Numerical multiple response optimization was applied to select an optimized formula (OF) with the goals of minimizing PS and maximizing ZP absolute value and EE. Box-Cox transformation was adopted to enable modeling PS raised to the power of 1.2 with an excellent prediction R of 1.000. ZP and EE were adequately represented directly with prediction R of 0.9549 and 0.9892 respectively. Response surface plots helped in explaining the influence of IVs on each DV. Two-sided 95% prediction interval test and percent deviation of actual values from predicted ones proved the validity of the elucidated models. The OF was a transfersomal formula with desirability of 0.866 and showed promising results in ex-vivo permeation study.

Sayed, S., I. Elsayed, and M. M. Ismail, "Optimization of β-cyclodextrin consolidated micellar dispersion for promoting the transcorneal permeation of a practically insoluble drug.", International journal of pharmaceutics, vol. 549, issue 1-2, pp. 249-260, 2018 Oct 05. Abstract

Development of efficient ocular drug delivery system for antifungal drugs becomes a must nowadays to face and eradicate the widely spread ophthalmic fungal infections. Itraconazole, a triazole antifungal, is struggling to penetrate the cornea and subsequently, its efficacy is limited. The aim of this study was to enhance itraconazole corneal penetration through utilizing the minimum surfactant amount in presence of β-cyclodextrin which acted as a dissolution and permeation enhancer. β-Cyclodextrin consolidated micellar dispersions (CCMD) were prepared after an initial screening to select the composition of surfactant(s). The preparation was done according to a modified melt dispersion technique. The prepared CCMD were characterized through the analysis of their particle size, zeta potential and solubilization efficiency. The optimum formula was chosen based on a factorial response surface analysis and it was composed of 17:1 w/w surfactant/drug, 30:1 w/w cyclodextrin/drug ratios and 0.02% polyethylene oxide. This formula was subjected to in vitro characterization including release, imaging by transmission electron microscope, mucoadhesion, stability, in addition to the determination of the minimum inhibitory concentration. Moreover, the ex vivo/in vivo permeation, safety and efficacy profiles were determined. The optimized CCMD formula was found to be significantly safe, stable, mucoadhesive and efficient to permeate the drug through rabbits' corneas. Consequently, the optimized CCMD formulation can be a promising, safe and efficient platform for the transcorneal delivery of lipophilic drugs including most antifungals.

Sayed, S., B. A. Habib, and G. M. Elsayed, "Tri-block co-polymer nanocarriers for enhancement of oral delivery of felodipine: preparation, in vitro characterization and ex vivo permeation.", Journal of liposome research, pp. 1-11, 2017 May 25. Abstract

This study aimed to prepare, optimize and characterize novel felodipine-loaded polymeric nanomicelles, using a pluronic mixture of F127 and P123. Thin-film hydration method was adopted for the preparation of different polymeric nanomicelles (T1-T12) according to a 41.31 full factorial design. Factors studied were: Pluronic®:drug ratio (P:D ratio) (10, 20, 30 and 40 w/w) and percent of hydrophilic polymer (F127%) (33.33%, 50% and 66.67% w/w). Optimization criteria were to maximize transmittance percent (T%) and entrapment efficiency percent (EE%) and to minimize particle size (PS) and polydispersity index (PDI). The optimized formulation was further characterized by DSC, FTIR and 1H NMR studies. It was also subjected to stability testing and ex vivo permeation using rabbit intestines. Spherical nanomicelles of particle size ranging from 26.18 to 87.54 nm were successfully obtained. The optimized formulation was found to be the already prepared formulation T12 (P:D ratio of 40 and 66.67% F127) with suitable T% and EE% of 95.12% and 91.75%, respectively. DSC, FTIR and 1H NMR studies revealed felodipine (FLD) incorporation within T12 nanomicelles. T12 enhanced the ex vivo intestinal permeation of FLD when compared to a drug suspension and showed good stability. Therefore, pluronic nanomicelles could be promising for improved oral delivery of FLD.

Elsayed, I., and S. Sayed, "Tailored nanostructured platforms for boosting transcorneal permeation: Box–Behnken statistical optimization, comprehensive in vitro, ex vivo and in vivo characterization", International Journal of Nanomedicine, vol. 12: Dove Medical Press, pp. 7947 - 7962, 2017/10/30. Abstracttailored_nanostructured_platforms_for_boosting_transcorneal_permeation_box-behnken_statistical_optimization.pdfWebsite

Ocular drug delivery systems suffer from rapid drainage, intractable corneal permeation and short dosing intervals. Transcorneal drug permeation could increase the drug availability and efficiency in the aqueous humor. The aim of this study was to develop and optimize nanostructured formulations to provide accurate doses, long contact time and enhanced drug permeation. Nanovesicles were designed based on Box–Behnken model and prepared using the thin film hydration technique. The formed nanodispersions were evaluated by measuring the particle size, polydispersity index, zeta potential, entrapment efficiency and gelation temperature. The obtained desirability values were utilized to develop an optimized nanostructured in situ gel and insert. The optimized formulations were imaged by transmission and scanning electron microscopes. In addition, rheological characters, in vitro drug diffusion, ex vivo and in vivo permeation and safety of the optimized formulation were investigated. The optimized insert formulation was found to have a relatively lower viscosity, higher diffusion, ex vivo and in vivo permeation, when compared to the optimized in situ gel. So, the lyophilized nanostructured insert could be considered as a promising carrier and transporter for drugs across the cornea with high biocompatibility and effectiveness.