Sherine S. Diab

Three green, simple, precise, accurate and sensitive spectrophotometric methods were developed for the determination of a binary mixture of lidocaine hydrochloride (LDC) and cetylpyridinium chloride (CPC) in the presence of dimethylaniline (DMA). In the three methods, the interference of DMA spectrum is eliminated using the ratio subtraction method. Method (A) depended on determining LDC and CPC by measuring the first derivative of the ratio spectra (DD) at 271.0 and 268.4 nm, respectively. Method (B) was the ratio difference (RD), based on dividing the absorption spectrum of the binary mixture by a standard spectrum of CPC or LDC, then measuring the amplitude difference of the ratio spectra (∆P) between 231.2 and 240.0 nm for LDC and between 242.8 and 258.0 nm for CPC. Method (C) based on the application of dual wavelength coupled with the isoabsorptive point method. This was achieved by measuring the absorbance difference (∆A) between 243.0 and 268.6 nm for the determination of LDC, followed by application of isoabsorptive point method comprised of measurement the total content of the mixture of LDC and CPC at their isoabsorptive point at 240.0 nm. The content of CPC was obtained by subtraction. The specificity of the developed methods was investigated by analyzing laboratory prepared mixtures containing different ratios of LDC and CPC in presence of DMA. The proposed methods displayed useful analytical characteristics for the determination of LDC and CPC in bulk powder and their combined dosage form. The obtained results were statistically compared with those obtained by the official methods, showing no significant difference with respect to accuracy and precision.

Four, accurate, precise, and sensitive spectrophotometric methods are developed for the simultaneous determination of a ternary mixture containing amlodipine besylate (AM), olmesartan medoxomil (OL) and hydrochlorothiazide (HZ), where AM is determined at its λmax 364.6 nm (0D), while (OL) and (HZ) are determined by different methods. Method (A) depends on determining OL and HZ by measuring the second derivative of the ratio spectra (2DD) at 254.4 and 338.6 nm, respectively. Method (B) is first derivative of the double divisor ratio spectra (D-1DD) at 260.4 and 273.0 nm for OL and HZ, respectively. Method (C) based on successive spectrophotometric resolution technique (SSRT). The technique starts with the ratio subtraction method then measuring OL and HZ at their isoabsorptive point at 260.0 nm, while HZ is measured using the amplitude of first derivative at 335.2 nm. Method (D) is mean centering of the ratio spectra (MCR) at 252.0 nm and 220.0 nm for OL and HZ, respectively. The specificity of the developed methods is investigated by analyzing laboratory prepared mixtures containing different ratios of the three drugs and their combined dosage form. The obtained results are statistically compared with those obtained by the official or reported methods, showing no significant difference with respect to accuracy and precision at p = 0.05.