Molecular Markers Associated with Salt Tolerance in Egyptian Wheats

Moghaieb, R. E. A., A. - H. A. Abdel-Hadi, and N. B. Talaat, "Molecular Markers Associated with Salt Tolerance in Egyptian Wheats", African Journal of Biotechnology, 2011.


Salinity affects plant growth by the osmotic stress of the salt around the roots, as well as by toxicitycaused by excessive accumulation of salt in leaves. In the present study, seven common (Triticumaestivum) and two durum (T. turgidum ssp. Durum) wheat genotypes were subjected to salt stress for 2weeks. Salt stress decreased leaf osmotic potential in all cultivars. The difference in osmoticadjustment between the cultivars was correlated with the concentrations of minerals examined such asNa+ and K+. The salt tolerance in the T. aestivum cultivar Gemmiza 10 and in T. durum cultivars Sohagand BeniSweif was due to higher ability to maintain osmotic potential of the cells than the othercultivars by increase in osmoticum concentration under salt stress. The genetic variation andrelationships among different wheat genotypes with different responses to salt stress were alsoinvestigated by RAPD and SSR analyses. 82 out of 118 RAPD markers detected were polymorphic(69.5%) and 42 out of 59 SSR alleles were polymorphic (71%), and can be considered as useful markersfor the wheat cultivars tested. 18 random amplified polymorphic DNAs (RAPD) markers and 13 simplesequence repeats (SSR) markers generated were found to be genotype-specific. Seven markersdistinguished the cultivar BeniSweif, six markers for the cultivar Sohag and two markers for thecultivar Gemmiza 10. These markers can be verified as being genetic markers associated with salt