Basic Science Sector

Citation:

Mohammadi-Sangcheshmeha, A., E. Heldaa, N. Ghanem, F. Rings, D. Salilew-Wondim, D. Tesfaye, H. Sieme, K. Schellander, and M. Hoelker, "G6PDH-Activity In Equine Oocytes Correlates with Morphology, Expression of Candidate Genes For Viability, and Preimplantative in Vitro Development", Theriogenology, 2011.

Abstract:

Efficiencies for in vitro production of equine embryos are still low due to highly variable developmental competences of equine immature oocytes. In contrast to the equine, in vitro developmental competence of immature oocytes has been predicted successfully by the activity of glucose-6-phosphate dehydrogenase (G6PDH) indicated by brilliant cresyl blue (BCB) dye in a range of different species. Therefore, the aim of the present study was to test the association between G6PDH activity in equine oocytes with: (1) cumulus morphology and oocyte properties in terms of diameter and volume; (2) maturational competence; (3) gene expression of certain molecular markers; and (4) in vitro embryo development after intracytoplasmic sperm injection. Equine oocytes were exposed to BCB

Notes:

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