Detection of Mycoplasma bovis and Mycoplasma bovigenitalium in Cattle andBuffalo in Egypt Using Dot ELISA and PCR with Anti -Microbial Trials

Citation:
SA., Marouf, K. F. Mohamed, J., and E. L. - J. akee, "Detection of Mycoplasma bovis and Mycoplasma bovigenitalium in Cattle andBuffalo in Egypt Using Dot ELISA and PCR with Anti -Microbial Trials", European Journal of Biological Sciences, vol. 25, pp. 136-146, 2011.

Abstract:

Mycoplasmae have the ability to colonize the respiratory and reproductive tracts of bovine. Consequently, these microorganisms can be associated with infertility and pneumonia. A total of 555 cows and 130 buffalo's samples (nasal swabs, lung tissues, tracheal swabs, bronchial lymph nodes and vaginal swabs) was collected from different Egyptian governorates and investigated. Mycoplasmaisolates collected from the respiratory tract of cattle and buffaloes were typed as M. bovis(2.7 and 1.7%, respectively) and other Mycoplasma species (10.8 and 4.2%, respectively). M. boviswas typed from vaginal swabs of the examined cows and buffaloes with incidence of 2.2 and 10 %, respectively, while the incidence of M. bovigenitaliumwas 13.3 and 10 %, respectively. Treatment of Mycoplasma diseases is difficult since Mycoplasma species lack a cell wall and are thus resistant to some commonly used antibiotics. Exposure of M. bovisand M. bovigenitaliumisolates to 1.1Ie against five antibiotics including erythromycin, gentamicin, tetracycline, streptomycin and lincomycin in comparison to reference strains revealed that lincomycin, erythromycin and streptomycin are more effective and able to get rid of M. bovisand M. bovigenitalium.

Notes:

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