Comparative Molecular and Conventional Detection Methods of BabesiaEqui (B

Citation:
Ibrahim, A. K., I. S. Gamil, A. - E. A. A. baky, M. M. Hussein, and A. A. Tohamy, "Comparative Molecular and Conventional Detection Methods of BabesiaEqui (B", Global Veterinaria, vol. 7, issue 2, pp. 201-210, 2011.

Abstract:

Theileriaequi is a tick-borne hemoprotozoan parasite and one of the causative agents of equinepiroplasmosis. Blood is collected from a total of 100 horses. Three diagnostic methods: microscopicalexamination, enzyme linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR), were comparedin detecting T. equi. The results showed that the prevalence rate of T. equi in examined horses was 18%, 30%and 26% by using microscopic examination, ELISA and PCR, respectively.Clinical, hematological andbiochemical studies were evaluated in examined horses. Significant decrease in hematological parameters wasobserved along with an increase in some biochemical parameters. The results of PCR assay showed itsimportance in the diagnosis of the carrier infected cases more than with the conventional techniques.The microscopic examination was used only in acute cases of the disease.

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