Chlamydophila psittaci and Chlamydophila Pecorum Infections in Goats and Sheep
- Citation:
- Osman, K. M., H. A. Ali, J. A. ElJakee, and H. M. Galal, "Chlamydophila psittaci and Chlamydophila Pecorum Infections in Goats and Sheep", Scientific and Technical Review, vol. 30, issue 3, pp. 393-348, 2011.
Abstract:
The aim of this study was to determine the epidemiology of chlamydiosis in free ranging asymptomatic and diarrhoeic sheep and goats. Fecal swabs were examined for the presence of chlamydiae by culture in Vero cells, chick embryo, Gimenez staining, direct fluorescein-conjugated monoclonal antibody staining and immunoperoxidase. Chlamydial DNA was then used to identify specific DNA by omp2 gene using the PCR-RFLP. The asymptomatic goats were 50% positive for the presence of the omp2 gene of the family Chlamydiaceae which were totally Cp. psittaci (100%). The percentage of the family Chlamydiaceae in diseased goats was 16.2% which were also identified to be positive for Cp. psittaci (100%). The asymptomatic sheep revealed that 6.7% were positive for the presence of the omp2 gene of the family Chlamydiaceae and to be 100% positive for Cp. psittaci. On the other hand, 42.9% of the samples that were collected from the diseased sheep were positive for the family Chlamydiaceae of which 25.7% were Cp. psittaci and 4.8% Cp. pecorum.
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