Basic Science Sector

Citation:

Hussiena, E. M., M. M. Ibrahima, Y. M. Issab, F. M. Abdel-Gawada, and S. Barakata, "Application of TLC- Densitometry, First Derivative and Ratio Derivative Spectrophotometry for the Determination of Eprosartan Mesylate and Hydrochlorothiazide in Pharmaceutical Preparations", International Journal of Drug Development and Research, 2011.

Abstract:

Three sensitive and accurate methods are described for the direct determination of eprosartan mesylate (EPM) and hydrochlorothiazide (HCT) in bulk powder and combined dosage form without prior separation. The first method is a first derivative spectrophotometry (1D) using a zerocrossing technique of measurement at 240.7 nm for EPM and at 233.4 nm for HCT. The second method is the first derivative of ratio spectrophotometry (1DD) where the amplitudes were measured at 237.0 nm for EPM and at 277.0 nm for HCT. The third method is based on TLCseparation of the two drugs followed by densitometric measurements of their spots at 290 and 270

Notes:

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