Basic Science Sector

Three plant species (CakilemaritimaScop., Senecioglaucus L. and RumexpictusForssk) were selected from three blacksand habitats along the Mediterranean coast in Egypt for cytogenetic studies and to recognizes the mechanism by whichplants withstand high concentration of the absorbed radionuclides through determination of the electrophoratic bandingpattern of protein and amino acid profiles. The study showed that exposure of the study plant species to soil radionuclidescauses decrease in the percentage of prophase and prophase to metaphase ratio, while the percentage of anaphase and telophaseincreases with soil radioactivity. The results revealed chromosomal aberrations, e.g., C-metaphase, star metaphase,chromosome stickiness at metaphase stage, C-anaphase and chromosomal breaks at the different mitotic stages with fluctuationin the index of mitotic phases. High radionuclide content of plants causes alterations in the bands relative mobility andintensities, expression of new proteins and suppression of some proteins.

Amphetamines (AMP) are potent psychostimulants and commonly used drugs of abuse. Its chronic administration creates tolerance and addiction and also associated with neurotoxicity and hepatocellular damage through oxidative stress.

A series of novel polyfunctionalized acyclic and heterocyclic dye precursors andtheir respective azo (hydrazone) counterpart dyes and dye precursors based on conjugateenaminones and/or enaminonitrile moieties were synthesized. The dyes and their precursorsare based on 2-cyano-N-(3-cyano-4,5,6,7-tetrahydrobenzo[b]thiophen-2-yl)-acetamide,2-ethoxycarbonyl-N-(3-cyano-4,5,6,7-tetrahydrobenzo[b]thiophen-2-yl)

Mycoplasmae have the ability to colonize the respiratory and reproductive tracts of bovine. Consequently, these microorganisms can be associated with infertility and pneumonia. A total of 555 cows and 130 buffalo's samples (nasal swabs, lung tissues, tracheal swabs, bronchial lymph nodes and vaginal swabs) was collected from different Egyptian governorates and investigated. Mycoplasmaisolates collected from the respiratory tract of cattle and buffaloes were typed as M. bovis(2.7 and 1.7%, respectively) and other Mycoplasma species (10.8 and 4.2%, respectively). M. boviswas typed from vaginal swabs of the examined cows and buffaloes with incidence of 2.2 and 10 %, respectively, while the incidence of M. bovigenitaliumwas 13.3 and 10 %, respectively. Treatment of Mycoplasma diseases is difficult since Mycoplasma species lack a cell wall and are thus resistant to some commonly used antibiotics. Exposure of M. bovisand M. bovigenitaliumisolates to 1.1Ie against five antibiotics including erythromycin, gentamicin, tetracycline, streptomycin and lincomycin in comparison to reference strains revealed that lincomycin, erythromycin and streptomycin are more effective and able to get rid of M. bovisand M. bovigenitalium.

Novel and effective electrochemical sensor for the determination of morphine (MO) in 0.04 mol L-1 universal buffer solution (pH 7.4) is introduced using gold nanoparticles electrodeposited on a Nafion modified carbon paste electrode. The effect of various experimental parameters including pH, scan rate and accumulation time on the voltammetric response of MO was investigated. At the optimum conditions, the concentration of MO was determined using differential pulse voltammetry (DPV) in a linear range of 2.0 x 10-7 to 2.6 x 10-4 mol L-1 with a correlation coefficient of 0.999, and a detection limit of 13.3 x 10-10 mol L-1, respectively. The effect of common interferences on the current response of morphine namely ascorbic acid (AA) and uric acid (UA) is studied. The modified electrode can be used for the determination of MO spiked into urine samples, and excellent recovery results were obtained.

A mixture of polyphenol components was isolated from the fruits of C. annuum L. cv. Cupra, C. annuum L. cv. Orange glory, and C. annuum L. cv. ST4712 (CLST), via 70% methanol extraction followed by column chromatography over silica gel. The polyphenol components of the mixture were analyzed via HPLC-MS/MS and compared with the reported data. Three cinnamic acid derivatives and five flavonoid components in the fruits of the three varieties were identified for the first time in this study. The antioxidant activity and anticancer effect of the polyphenol mixtures of the three fruits were determined. The antioxidant and anticancer activities of CLST were substantially higher than those of C. annuum L. cv. Cupra and C. annuum L. cv. Orange glory. The high activities of CLST were attributed to the much higher concentration of quercetin derivatives in CLST.

Spinetoram is a second-generation member of the spinosyn class, all members of which have been shown to be effective in insect control via a novel mode of action. Spinetoram is a mixture of 3'-O-ethyl-5, 6-dihydro spinosyn J (XDE-175-J) and 3'-O-ethyl spinosyn L (XDE-175-L). In order to establish a determination method for the analysis of spinetoram residues in crops, commercial product (5% suspension concentrate spinetoram) was applied to two leafy vegetables (Garland chrysanthemum and Aster scaber) on different spraying schedules. The analytical method used herein was based on a reversed-phase separation on a C(18) column, isocratic elution and UV detection. The analytes were confirmed via tandem mass spectrometry. The method was linear over a concentration range of 0.05-10 ppm with a correlation coefficient in excess of 0.9998. The recoveries of XDE-175-J and XDE-175-L from the two vegetables ranged between 86.04 and 98.87% at spiking levels of 1 and 5 ppm. The relative standard deviations were no more than 7% for all recovery tests conducted herein. The calculated limits of detection and quantification were 0.01 and 0.03 ppm for both XDE-175-J and XDE-175-L. The levels of residues in two vegetables treated under a fixed schedule in the greenhouse were 6.21-0.55 ppm (maximum residue limit (MRL) = 7 ppm). In sum, this method constitutes an easy and reliable technique for the determination of spinetoram in leafy vegetables.

In this study, a multiresidue analytical method for the detection of 37 pesticides in a soil matrix was developed and validated. The soil sample was fortified with a known quantity of pesticides at two different concentration levels (0.1 and 0.01 µg/g) and the analytes were extracted via a liquid–solid extraction method. The pesticides were separated on an HP5 capillary column and were analyzed with a gas chromatograph coupled to a nitrogen–phosphorous detector (GC-NPD). Method validation was accomplished with good linearity (r2 = 0.994–0.999) within a considerable range of concentrations. Satisfactory recoveries (70.5–110.4%) were obtained with 32 pesticides at both spiking concentration levels, whereas five pesticides—dimepiperate, buprofezin, prometryn, pirimicarb, and fludioxonil—were recovered at relatively low levels (43.6–61.8%). The applicability of the method was demonstrated by analyzing field samples collected from 24 different sites around Yeongsan and Sumjin rivers in the Republic of Korea. No residues of the selected pesticides were detected in any of the samples. The developed method could be employed as a simple and cost-effective method for the routine detection and analysis of 37 pesticides in soil samples.

The acidic herbicides are an important class of chemical compounds that are used to control a variety of weeds that threaten many crops. Owing to their low microbial activity levels, the acidic herbicides exhibit a residual activity remaining for periods of up to several months in soils and water. The principal objective of this study was to develop an analytical method based on liquid–liquid and solid-phase extraction followed by HPLC, for the determination of 2,4-D in paddy field water. The residues were verified via tandem mass spectrometry (MS/MS) in negative-ion electrospray ionization (ESI) mode. Linearity was good over a concentration range of 1–100 µg/L with a correlation coefficient (r2) of 0.999. The mean recovery rates of triplicate results ranged from 85.2 to 90.85%. The limits of detection and quantitation were 0.4 and 1.0 µg/L, respectively. The method proposed herein was applied to field samples acquired from Hampyung and Sunchang counties, Republic of Korea. The analyte was detected at a concentration range of 6.8–12.8 and 3.55–24.0 µg/L, respectively.

In this study, we describe a newly developed and simple analytical method using high-performance liquid chromatography coupled to fluorescence detector (HPLC-FLD) for the determination of enrofloxacin, ciprofloxacin, and danofloxacin in porcine plasma. A single-step sample preparation, including extraction with acidic acetonitrile, coagulation with ammonium acetate, and centrifugation made possible the direct analysis of plasma samples without the need for any further cleanup procedure. The developed method was validated with specificity, linearity, limit of detection (LOD), limit of quantitation (LOQ), accuracy, and precision. All results were fully adequate. In the study using porcine plasma incurring enrofloxacin, the developed method proved capable of quantifying concentrations below its maximum residue limit, and its time-course residues were excreted within the 10-day withdrawal time.

To prevent engine failure it is essential to change lubricating oil regularly before it loses its protective properties. It is also necessary to monitor the physical and chemical conditions of the oil to reliably determine the optimum oil-change intervals. The present work focuses on studying evolution of the cyanide (CN) and carbon (C2) molecular spectral emission lines in the laser induced breakdown spectra of lubricating oil as a function of its consumption.

This study was conducted to examine the efficacy of fish oil supplementation in male wistar rat colon carcinogenesis.In order to induce colon cancer, the rats were given a weekly subcutaneous injection of 1,2-Dimethyl hydrazine (DMH) at a dose of 20 mg/kg b.w. for five weeks. Afterwards, some of the rats ingested fish oil for either 4 weeks (DMH-FO4 group), or 17 weeks (DMH-FO17 group). The remaining rats continued without any supplementation for the same 4 weeks (DMH4 group), or 17 weeks (DMH17 group). Another two groups of rats did not receive the DMH and were given fish oil (FO17 group) or a normal diet only and considered as the control group (CN group). At the end of the experiment, the rats were sacrificed; and were subsequently subjected to biochemical and molecular biological analyses as well as histopathological examinations. The results showed increased levels of lactate dehydrogenase (LDH), malondialdehyde (MDA) and alkaline phoshatase (ALP) activities in the DMH rats compared to the control. The liver and colonic changes that were induced by DMH were significantly improved through fish oil supplementation in the DMH-FO17 group. The molecular analysis revealed that DMH treatment induced the expression alterations of genes p53, p27 and p21 and increased DNA band patterns related to cancer, while both FO17 and DMH-FO17 groups showed much better results. A histopathological examination of the DMH17 group revealed colon adenocarcinoma and several lesions in rat liver tissues. An improvement in the histopathological picture was seen in the livers and colons of groups DMH- FO17. In conclusion, the present results demonstrated the anti-carciongenic effect of herring fish oil against DMH induced colon carcinogenesis in rats. The inhibitory effect ofFO was due to the modulation of elevated biochemical parameters, DNA damage and histopathological lesions caused by DMH.

Rapeseed is one of the most important oilseed crops cultivated in many parts of the world and used as a source of edible oil for human consumption. The present work reports some phytochemical and nutritional potentials of different rapeseed cultivars with a view to exploit their benefits for human nutrition. Five rapeseed cultivars namely; pactol, silvo, topas, serw 4 and serw 6 were analyzed for their phytochemical constituents (such as glutathione, ascorbate, phenolic, flavonoid contents) and some antioxidant enzymes activity (i.e. GPX; guaiacol peroxidase, APX; ascorbate peroxidase, CAT; catalase, SOD; superoxide dismutase) together with their protein profile. Among all cultivars significant variations in glutathione, ascorbate, phenolic and flavonoid contents were observed. The highest DPPH radical scavenging activity of the extract was observed in topas cultivar. Minor variations were noticed in SDS-PAGE protein profile. The results of the study suggest the phytochemical and nutritional potentials of B. napus seeds for human and other animal uses due to their extensive consumption in the world

To study the effect of different concentration of lead in drinking water on nucleic acid contents, nuclease activities and GOT in different tissues. It was attempts to reduce toxic effects of lead on survive of the environment especially human and rats by using pectin and chitin natural compounds in rat diets.

Dispersal ability and field progeny production of augmentative released biological control agents depend on ecological adaptations of the particular species or strains used. Four species of the egg parasitoid genus Trichogramma were compared aiming to select suitable candidates for control of lepidopteran olive pests.

The reaction kinetics of epoxy resin cured with stoichiometric amounts of amine-like structure and loaded with different levels of Cloisite 25A (C25A), has been studied by dynamic differential scanning calorimetry (DSC) to investigate the effect of the nano-organoclay particles on the kinetic parameters of the cure reaction.

Aspartame is one of the most widely used artificial sweeteners in over 90 countries worldwide. It is ahighly intensity sweetener added to a large variety of food, most commonly found in low caloriebeverages, desserts and table top sweeteners added to tea or coffee. The present study examinedwhether the daily oral administration of ASP (40 mg/kg) for 2, 4 and 6 weeks induce oxidative stress inthe liver and kidney of male albino rats. Lipid peroxidation (LPO), glutathione reduced (GSH) levels aswell as the activities of superoxide dismutase (SOD), glutathione-S-transferase (GST) and catalase (CAT) enzymes were determined. A significant increase in LPO levels was obtained in the liver tissueafter 4 and 6 weeks of ASP administration while there was a significant decrease in LPO level after 2weeks followed by a significant increase in the renal tissue at the end of the 6 weeks. SOD activitysignificantly decreased in the liver tissue after 2, 4 and 6 weeks of treatment. Also, there was asignificant decrease in SOD activity after 2 and 4 weeks in the renal tissue. CAT activity significantly decreased in the liver tissue after 2 and 4 weeks of ASP administration. Regarding to GSH content, there was a significant decrease in the liver tissue after 2, 4 and 6 weeks which was accompanied by asignificant increase in GST activity after 4 and 6 weeks of ASP administration. In conclusion, ASP may induce an oxidative stress in the liver and kidney of male albino rats.

The present study aimed to investigate the possibility of involved oxidative stress due to the oraladministration of either ciprofloxacin or levofloxacin (under therapeutic level) in the three brain regions, cerebral cortex, hippocampus and striatum of male albino rats weighing

As incidence of diabetes is increasing worldwide, besides it is associated with complications, the present studyaimed to investigate the effect of a traditional botanical, ginseng on diabetes induced alterations in kidneyfunctions. Thirty male rats were used in the study by randomly allocating them into three groups, each of ten rats,namely the control group, diabetes group (D), and diabetes+ ginseng group (DG). The latter two groups wererendered diabetic by I/P injection of streptozotocin (50 mg/kg). Daily ginseng extract was administered orally 100( mg/kg BW), one week post streptozotocin (STZ) injection. Ninety days post STZ injection; rats weresacrificed, where serum and kidneys were obtained for determination of metabolic profile, serum electrolytes,kidney function tests, renal tissue enzymes, and renal antioxidant status, together with histopathology. Theobtained results revealed a modest improvement in metabolic profile due to ginseng extract administration.However, the kidney functions were greatly improved as evidenced by amelioration of urea nitrogen, creatinine,total protein concentrations and serum electrolytes. Also an increase was noted in renal tissue enzymes andantioxidants with a decrease in malondialdehyde and renal pathology. In conclusion, ginseng extract may be ofsupportive treatment to combat diabetes complications.