PCR Assays for Detecting Major Pathogens of Mastitis in Milk Samples
- Citation:
- Amin, A. S., R. H. Hamouda, and A. A. A. Abdel-All, "PCR Assays for Detecting Major Pathogens of Mastitis in Milk Samples", World Journal of Dairy & Food Sciences, 2011.
Abstract:
Bovine milk samples were collected from cases of clinical and sub-clinical mastitis, respectively andexamined bacteriologically and by simplex and multiplex PCR assays for detection of Staphylococcus aureus,Escherichia coli and Streptococcus agalactiae. Escherichia coli was the most common bacteria detected inthe collected samples from clinical mastitis cases followed by Staphylococcus aureus and Streptococcusagalactiae; while Staphylococcus aureus was the most common bacteria followed by Escherichia coli andStreptococcus agalactiae in samples collected from sub-clinical mastitis cases. Compared with the culturalmethod, the simplex and multiplex PCR assays are less time consuming. It took less than 24 hours to becompleted, while identification of bacteria to the species levels by conventional microbiological and biochemical
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