Ouf, S. A., J. Rasheed, K. Ahmad, Z. Khan, K. Wajid, M. Nadeem, S. Tehreem, T. Abbas, H. Bashir, A. Ashfaq, et al., Nutritional Imbalance, Toxicology and Deficiency Potential of Livestock, , vol. 71, pp. 51 - 62, 2020/12/04. Abstract

This study investigated the changes in the enzymatic and biochemical profiles of lactating, non-lactating, and young buffaloes during different sampling. In the present research thirty buffaloes (Nili-Ravi) were selected and divided into three categories lactating, non-lactating, and young. Four samplings were performed in different seasons (summer, autumn, winter, and spring), and 10 blood serum samples were collected from each category of Nili-Ravi buffaloes during each sampling period. Higher glucose, urea, creatinine, and mycotoxins (AFB1, ZEA, OTA) values were found during summer sampling season, higher SGPT (ALT) and SGOT (AST) values were found during the autumn season, higher cholesterol, alkaline phosphatase and uric acid values were found during the winter season.

Ouf, S. A., J. Rasheed, K. Ahmad, Z. Khan, K. Wajid, M. Nadeem, S. Tehreem, T. Abbas, H. Bashir, A. Ashfaq, et al., Nutritional Imbalance, Toxicology and Deficiency Potential of Livestock, , vol. 71, pp. 51 - 62, 2020/12/04. Abstract

This study investigated the changes in the enzymatic and biochemical profiles of lactating, non-lactating, and young buffaloes during different sampling. In the present research thirty buffaloes (Nili-Ravi) were selected and divided into three categories lactating, non-lactating, and young. Four samplings were performed in different seasons (summer, autumn, winter, and spring), and 10 blood serum samples were collected from each category of Nili-Ravi buffaloes during each sampling period. Higher glucose, urea, creatinine, and mycotoxins (AFB1, ZEA, OTA) values were found during summer sampling season, higher SGPT (ALT) and SGOT (AST) values were found during the autumn season, higher cholesterol, alkaline phosphatase and uric acid values were found during the winter season.

Ouf, S. A., S. M. Gomha, M. Eweis, A. S. Ouf, I. A. A. Sharawy, and S. A. Alharbi, "Antidermatophytic activity of some newly synthesized arylhydrazonothiazoles conjugated with monoclonal antibody.", Scientific reports, vol. 10, issue 1, pp. 20863, 2020. Abstract

A new series of 5-arylhydrazonothiazole derivatives 5a-d has been synthesized, elucidated, and evaluated for their antidermatophytic activity. The minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) of the newly synthesized products were investigated against 18 dermatophyte fungal isolates related to Epidermophyton floccosum, Microsporum canis, and Trichophyton rubrum. The morphological alterations induced by the synthesized derivatives singly or conjugated with the monoclonal antibody were examined on spores of T. rubrum using a scanning electron microscope. The efficacy of synthesized derivative 5a applied at its respective MFC alone or conjugated with anti-dermatophyte monoclonal antibody 0014 in skin infection treatment of guinea pigs due to inoculation with one of the examined dermatophytes, in comparison with fluconazole as standard reference drug was evaluated. In an in vivo experiment, the efficiency of 5a derivative conjugated with the antibody induced 100% healing after 45 days in the case of T. rubrum and M. canis-infected guinea pigs.

Ouf, S. A., and E. Ali, Does the treatment of dried herbs with ozone as a fungal decontaminating agent affect the active constituents?, , vol. 277, pp. 116715, 2021/02/01. Abstract

Herbs and spices are food crops susceptible to contamination by toxigenic fungi. Ozone, as a decontamination approach in the industry, has attractive benefits over traditional food preservation practices. A contribution to the studying of ozone as an antifungal and antmycotoxigenic agent in herbs and spices storage processes is achieved in this research. Nine powdered sun-dried herbs and spices were analyzed for their fungal contamination. The results indicate that licorice root and peppermint leaves were found to have the highest population of fungi while black cumin and fennel record the lowest population. The most dominant fungal genera are Aspergillus, Penicillium, Fusarium, and Rhizopus. Ozone treatment was performed at a concentration of 3 ppm applied for exposure times of 0, 30, 90, 150, 210, and 280 minutes. After 280 minutes of exposure to ozone, the reduction of fungal count ranged from 96.39 to 98.26%. The maximum reduction in spore production was achieved in the case of A. humicola and Trichderma viride exposed for 210 minutes ozone gas. There was a remarkable reduction in the production of the total mycotoxin, reaching 24.15% in aflatoxins for the 150 minutes-treated inoculum in the case of A. flavus. The total volume of essential oil of chamomile and peppermint was reduced by 57.14 and 26.67%, respectively, when exposed to 3ppm.For 280 minutes
In conclusion, fumigation with ozone gas can be used as a suitable method for achieving sanitation and decreasing microbial load in herbs and spices. Still, it is crucial to provide precautions on ozone's effect on major active constituents before recommending this method for industrial application.

Alhilal, M., Y. Y. or Sulaiman, S. Alhilal, S. Gomha, and S. A. Ouf, Antifungal Activity of New Diterpenoid Alkaloids Isolated by Different Chromatographic Methods from Delphinium peregrinum L. var. eriocarpum Boiss, , vol. 26, pp. 1 - 10, 2021/03/04. Abstract
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Ouf, S. A., A. Q. Alsarrani, A. A. Al-Adly, M. K. Ibrahim, and M. H. Ismail, "Evaluation of low-intensity laser radiation on stimulating cholesterol degrading activity of Streptomyces fradiae Part III cholesterol oxidase purification and application", African Journal of Microbiology Research, vol. 6, issue 7, no. 7: Academic Journals, pp. 1509–1519, 2012. Abstract

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Ouf, S. A., T. A. Moussa, A. M. Abd-Elmegeed, and S. R. Eltahlawy, "Anti-fungal potential of ozone against some dermatophytes", Brazilian Journal of Microbiology, vol. 47, issue 3: Elsevier, pp. 697-702, 2016. Abstract

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Khalil, N. K., M. I. Ali, S. A. Ouf, and M. N. Abd El-Ghany, "Characterization of Aspergillus flavus NG 85 laccase and its dye decolorization efficiency. ", Research Journal of Pharmaceutical, Biological and Chemical Sciences , vol. 7, issue 4, pp. 829-817, 2016.
Ali, M. I. A., S. A. Ouf, N. M. Khalil, and M. N. Abd El-Ghany, "Biosynthesis of laccase by Aspergillus flavus NG85 isolated from Saint Catherine protectorate.", Egyptian Journal of Botany, vol. 55, issue 1, pp. 127-147, 2015. Abstract

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Ouf, S. A., T. A. Moussa, A. M. Abd-Elmegeed, and S. R. Eltahlawy, "Dermatophytosis in special patient populations", Journal of Coastal life Medicine, vol. 4, issue 4, pp. 324-326, 2016. Abstract

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