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Soliman, L. A., R. A. Zayed, D. Omran, F. Said, S. K. Darweesh, D. M. Ghaith, R. E. Etreby, M. S. Barakat, M. M. Bendary, D. Z. Zaky, et al., "Apelin Association with Hepatic Fibrosis and Esophageal Varices in Patients with Chronic Hepatitis C Virus", American Journal of Tropical Medicine and Hygiene, vol. 107, issue 1, pp. 190-197, 2022.
Osman, R., S. Fathy, H. A. N. A. N. SHAFIK, N. Momen, R. Kandil, and R. Zayed, "Study of epigenetic regulation of H3K27me3 in acute myeloid leukemia", International Journal of Laboratory hematology, vol. 44, pp. e111-e114, 2022.
Zayed, R. A., D. Omran, and A. A. Zayed, "COVID-19 clinical and laboratory diagnosis overview ", Journal of the Egyptian Public Health Association, vol. 96, issue 1, pp. 25, 2021.
Zayed, R. A., Z. El-Saadany, H. A. N. A. N. N. O. U. R. RASLAN, M. Ghareeb, D. Ibraheem, M. Rashed, R. Kandil, and O. Y. Abdeldayem, "IL-17 A and IL-17 F single nucleotide polymorphisms and acute myeloid leukemia susceptibility and response to induction therapy in Egypt", Meta Gene, vol. 26, 2020.
Fateen, M. A., D. E. M. Demerdash, R. A. Zayed, and M. M. Mattar, "Role of physical function in predicting short-term treatment outcome in Egyptian acute myeloid leukemia patients: a single center experience ", Hematology, Transfusion and Cell Therapy, vol. 41, issue 1, pp. 17-24, 2019.
Wifi, M. - N., R. A. Zayed, N. E. V. I. N. E. FOUAD, ahmed y hassan, M. A. Hussien, and M. G. Sokar, "Association of serum growth differentiation factor 15 and hepatocellular carcinoma in Egyptian patients", The Egyptian Journal of Internal Medicine, vol. 31, pp. 57-63, 2019.
Youssef, R., O. A. M. Zeid, N. E. S. R. I. N. SAMIR, R. A. Zayed, and D. Omran, "Interferon alpha, gamma, and lambda 2: are they possible culprits in the association between psoriasis and hepatitis C virus?", Journal of the Egyptian Women's Dermatologic Society, vol. 16, pp. 51-55, 2019.
Attallah, A. M., M. S. Albannan, M. M. Omran, R. Zayed, S. Saif, A. Farid, M. Hassany, A. Yosry, and D. Omran, "A panel of a mitogenic (PDGF), biochemical (albumin) and demographic (age) parameters for the non-invasive assessment of hepatic fibrosis", Br J Biomed Sci., vol. 76, issue 3, pp. 105-110, 2019.
Zayed, R. A., S. A. Elhamid, and D. Khamess, "The Association of Transforming Growth Factor-β1 (TGF-β1) Gene Polymorphism and Susceptibility to Aplastic Anemia in Egyptian Patients", International Journal of Pharmaceutical and Clinical Research, vol. 10, issue 11, pp. 249-251, 2018. the_association_of_transforming_growth_factor-b1_tgf-b1_gene.pdf
Omran, D., A. Yosry, S. K. Darweesh, M. M. Nabeel, M. El-Beshlawey, S. Saif, A. Fared, M. Hassany, and R. A.Zayed, "Enhanced liver fibrosis test using ELISA assay accurately discriminates advanced stage of liver fibrosis as determined by transient elastography fibroscan in treatment naı¨ve chronic HCV patients", Clin Exp Med, vol. 18, issue 1, pp. 45-50, 2018.
Omran, D., R. A. Zayed, M. M. Nabeel, L. Mobarak, Z. Zakaria, A. Farid, M. Hassany, S. Saif, M. Mostafa, O. K. Saad, et al., "Evaluating Diagnostic Accuracy of Noninvasive Tests in Assessment of Significant Liver Fibrosis in Chronic Hepatitis C Egyptian Patients", Viral Immunol. , vol. 31, issue 4, pp. 314-320, 2018.
Attallah, A. M., D. Omran, M. M. Omran, M. A. Abdelrazek, R. Zayed, R. E. Essawey, S. Saif, A. Farid, M. Hassany, A. Yosry, et al., "Extracellular Matrix Proteins Substantiate IL-28B T allele Effect on Histological Outcome of Chronic Hepatitis C", Ann Hepatol. , vol. 17, issue 4, pp. 569-576, 2018.
Attallah, A. M., D. Omran, M. M. Omran, M. S. Albannan, R. A. Zayed, S. Saif, A. Farid, M. Hassany, and A. Yosry, "Fibro-Mark: a panel of laboratory parameters for predicting significant fibrosis in chronic hepatitis C patients", Br J Biomed Sci., vol. 75, issue 1, pp. 19-23, 2018.
Omran, D., M. Alboraie, R. A. Zayed, M. - N. Wifi, mervat naguib, M. Eltabbakh, M. Abdellah, A. F. Sherief, S. Maklad, H. H. Eldemellawy, et al., "Towards hepatitis C virus elimination: Egyptian experience, achievements and limitations", World J Gastroenterol , vol. 24, issue 38, pp. 4330-4340, 2018.
Abou-Elew, H. H., I. Youssry, S. Hefny, R. H. Hashem, N. E. V. I. N. E. FOUAD, and R. A. Zayed, "βS globin gene haplotype and the stroke risk among Egyptian children with sickle cell disease", Hematology, vol. 23, issue 6, pp. 362-367, 2018.
Zayed, R. A., D. Omran, D. A. Mokhtar, Z. ZAKARIA, S. Ezzat, M. A. Soliman, L. Mobarak, H. El-Sweesy, and G. Emam, "Association of Toll-Like Receptor 3 and Toll-Like Receptor 9 Single Nucleotide Polymorphisms with Hepatitis C Virus Infection and Hepatic Fibrosis in Egyptian Patients.", Am J Trop Med Hyg., vol. 96, issue 3, pp. 720-726, 2017.
Zayed, R. A., D. Omran, A. A. Zayed, and L. O. Elmessery, "Determinants of Infection Outcome in HCV-genotype 4", Viral Immunology, vol. 30 , issue 8, pp. 560-567, 2017.
Zayed, R. A., M. A. Eltaweel, S. K. Botros, and M. A. Zaki, "MN1 and PTEN gene expression in acute myeloid leukemia", Cancer Biomarkers, vol. 18, issue 2, pp. 177-182, 2017.
Zayed, R. A., S. M. Abdel-Hamid, and H. El-Lithy, "The association of cytokine genes polymorphisms and susceptibility to aplastic anemia in Egyptian patients", Hematology, vol. 21, issue 2, pp. 106-112, 2016.
Mansour, I., R. A. Zayed, F. Said, and L. A. Latif, "Indoleamine 2,3-dioxygenase and regulatory T cells in acute myeloid leukemia", Hematology, vol. 21, issue 8, pp. 447-453, 2016.
Zayed, R. A., M. El-Ghamrawy, H. A. Alwakeel, and N. Esh, "Impact of circulating erythrocyte-derived microparticles on coagulation activation in sickle cell disease", Comparative Clinical Pathology, vol. 24, issue 5, pp. 1123-1128, 2015.
Gabr, H., and R. A. Zayed, "Mesenchymal Stem Cell Infusion in Chronic Renal Failure Patients", Journal of Medical and Bioengineering, vol. 4, issue 4, pp. 329-331, 2015.
Omran, D., S. Hamdy, S. Tawfik, S. Esmat, D. ’aA. Saleh, and R. A. Zayed, "Association of Interferon-γ Inducible Protein-10 Pretreatment Level and Sustained Virological Response in HCV-Positive Egyptian Patients.", Annals of Clinical & Laboratory Science, vol. 44, issue 2, pp. 169-174, 2014. Abstract

Background. The response to antiviral therapy in HCV infected patients depends on several
predictive factors; however, the ability to achieve sustained virological response is still limited to around
60% of the patients infected with the HCV-4 genotype. Increased serum and intrahepatic interferon -γ
inducible protein 10 (IP-10) levels in patients with chronic hepatitis C have been described. The aim of
the work was to study the impact of pretreatment serum IP-10 level on the antiviral treatment outcome
in a group of Egyptian patients infected with HCV. Materials and methods. The study included 80 treatment
naive HCV patients. Serum IP-10 levels were determined by an enzyme linked immunosorbent assay
before therapy was introduced. Serum samples were examined twice by Real-Time PCR after complete
course of therapy for detection of HCV RNA; at the end of the antiviral therapy and six months later to
detect sustained virological response (SVR). Results. 57 patients (71%) achieved SVR while 23 (29%) patients were non-responders (NR). Pretreatment serum IP-10 levels were significantly lower in patients who achieved SVR than in NR (p=0.000). Conclusion. Low pretreatment serum IP-10 is a favorable predictive of response to antiviral HCV therapy in Egyptian patients.

Gabr, H., R. Zayed, A. ElBeshlawy, L. Hegazy, R. Fawzy, M. Samir, and H. Mosa, "Differentiation of bone marrow hematopoietic stem cells into FVIII-producing hepatocytes: approach to hemophilia treatment", Comp Clin Pathol , vol. 23, pp. 193-198, 2014. Abstract

Hemophilia is caused by a single-gene defect resulting in familial bleeding disorder. Small increase in
gene products could transform a severe form of hemophilia into a mild one. Stem cells from extrahepatic sources are being considered for clinical applications in liver cell therapy as they possess high in vitro culture potential and could be used in transplant procedures. We studied the differentiation
of bone marrow hemapoietic stem cells (BM-HSCs) from hemophilia patients' relatives into FVIII-producing hepatocytes aiming to expand patients' donor options for partial replacement of mutant liver cells by healthy cells in hemophilia A patients which could manage the severity of the bleeding disorder. BM-HSCs from hemophilic families were cultured in liquid culture containing hepatocyte growth factor for 6 days. Differentiation into hepatocytes was evaluated by alpha-fetoprotein (AFP) expression using mmunocytochemistry. Functional evaluation of transdifferentiation into hepatic lineage was done through albumin synthesis in culture supernatant using microalbumin assay kit, factor VIII activity by one stage clotting assay and expression of FVIII mRNA by RT-PCR. BM-HSCs-derived hepatocytes showed positive
AFP expression with a mean of 11 %. Functional tests performed showed their ability to produce albumin and perform FVIII activity. Also, FVIII mRNA expression was detected. Inducing the differentiation of BM-HSCs by in vitro manipulation may become a valuable tool to provide a cell source for hepatocyte transplant procedures for treatment of hemophilia patients.

El-Bassiouni, N. E., E. L. O. Messery, R. A. Zayed, O. B. Metwally, M. Y. Zahran, O. M. Mahmoud, R. A. Ibrahim, and E. A. E. Bassiouny, "Tissue factor expression on blood monocytes in patients with hepatitis C virus-induced chronic liver disease", Comp Clin Pathol , vol. 23, pp. 1159-1166, 2014. Abstract

Chronic liver disease (CLD) is a worldwide common pathology characterised by an inflammatory and fibrotic process leading to progressive evolution from chronic hepatitis to cirrhosis.Monocytes play a crucial role in the pathogenesis of inflammation and fibrosis in chronic liver diseases. Activated monocytes increase the expression of tissue factor, a key glycoprotein that participates in haemostatic and nflammatory processes. This study aims to assess the expression of tissue factor on activated peripheral blood monocytes in patients with hepatitis C virus (HCV)-induced CLD in relation to the degree of hepatic insufficiency and haemostatic imbalance. The current study included 60 patients with HCV induced
CLD, categorised after Child–Pugh into four groups: Child A, B, C and C during acute attack of haematemesis, 15 patients each, and 15 healthy subjectswere included as normal controls. Immunophenotype characterization was carried out by flow cytometric analysis for identification of monocytes tissue factor expression (CD142) on activated blood monocytes population (CD11b and CD14) in different groups studied. Data demonstrated significant increase (p<0.05) in the expression of each of CD11b, CD14 and CD142 revealing monocytes activation and increased expression of tissue factor
on peripheral blood monocytes in different groups of patients especially cases during acute attack of haematemesis, compared to healthy subjects. Increased monocytes tissue factor expression in patients with HCV may play a key role in the intensification of the inflammatory and immunological processes
in conjunction with activation of the coagulation mechanisms. The interaction of all these phenomena may trigger bleeding by perturbing the unstable haemostasis in frail patients with chronic liver disease.