Publications

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2023
Henawy, A. R., A. A. Abdelhadi, A. A. b Halema, R. I. Refae, and O. S. Barakat, "Exopolysaccharide production from agro-industrial wastes by lactic acid bacteria isolated from silage", Egyptian Pharmaceutical Journal, vol. 22, pp. 403–414, 2023.
2022
Alaa, S. A., R. I. Refae, A. A. Gamil, and R. E. TAREK, "CHARACTERIZATION AND POTENTIALS OF MICROBIAL COMMUNITY IN SOIL AND WATER CONTAMINATED WITH CRUDE OIL IN EGYPT", Egyptian Journal of Chemistry, vol. 65, issue 8, pp. 367-379, 2022.
2020
Nagwa, H., H. Ibrahim, I. R. Refaea, and M. Fayez, EL-SALAM CANAL WATER AUTOCHTHONOUS MICROBIOME SELF- BIOREMEDIATES THE ENTERIC PATHOGENIC BACTERIA AND SUPPORTS THE IN-SITU LETTUCE DEVELOPMENT, , vol. 20, pp. 5561 - 5569, 2020/10/02. Abstract

El-Salam canal water is planned to cultivate an acreage of 400,000 acre-1 located in north Sinai. In the present study, chemical (pH, EC, DO, BOD, COD, NH 4 , NO 2 , NO 3 , Ca, Mg, Na, K, SAR, Cd, Cu, Fe, Zn) and bacteriological (total bacteria, total and faecal coliforms) analyses were related to the permissible limits of FAO, WHO and Mediterranean countries for irrigation water. Representatives of pathogen and PGPR isolates were secured from the canal water and rhizospheres of some plants cultivated in El-Qantara Shark fields irrigated with such water. Based on biochemical properties and 16S rRNA gene sequencing, the pathogens were identified as Escherichia fergusonii, Klebsiella pneumoniae and Shigella sonnei while PGPR were belonging to Bacillus altitudinis, Brevibacillus brevis, Paenibacillus xylanexedens and Stenotrophomonas maltophilia. These strains did possess the ability to produce the plant growth accelerating compounds, abscisic acid (15.0-45.0 µg/ 100ml), cytokinins (1.2-90.2 µg/100ml), gibberellins (2.7-4.0 mg/100ml), indole acetic acid (73.0-203.0 µg/100ml), and polysaccharides (1.1-5.6 mg/ml). They successfully antagonized the pathogen E. fergusonii in nutrient agar culture media. The lettuce growth traits were found to be PGPR inoculation-dependent rather than irrigation water type-dependent.

Maghrawy, H. H. B., O. Gomaa, H. A. E. Kareem, R. Refae, and S. ElGizawy, "Biocontrol Mechanisms of Bacillus sp. and its Application as a Bionanocomposite against Rhizoctonia solani", Journal of Nuclear Technology in Applied Science, vol. 8, issue 1, pp. 49-65, 2020.
Maghrawy, H. H., O. M. Gomaa, S. ElGizawy, R. I. Refae, and H. A. E. Kareem, "Production and characterization of antifungal compound from Bacillus sp. GRP against Rhizoctonia solani using bio-nano-fungicide", Middle East Journal of Applied Sciences, vol. 10, issue 2, pp. 279-293, 2020.
2019
El-Kader, S. F. A., G. A. El-Chaghaby, G. M. Khalafalla, R. I. Refae, and H. M. Elshishtawy, "A novel microbial consortium from sheep compost for decolorization and degradation of Congo red", Global Journal of Environmental Science and Management, vol. 5, issue 1, pp. 61-70, 2019.
2014
Refae, R. I., "Removal of heavy metals from solution using biological methods", TCSSE 3rd International Conference of Science and Technology, University of Central Florida, Florida, USA, 16th October, 2014.
2013
Ahmed, R. S., K. G. M. Allah, R. I. Refae, M. A. Ali, and M.H.Belal, "Biosorption of chrome and chemical oxygen demand reduction from tannery wastewater by aerobic activated sludge", Bull. Fac. Agric., Cairo Univ., vol. 64, pp. 204-209, 2013.
2011
Makbol, H., R. I. Refae, H. A. Eid, and O. M. Mohamed, "Biological activity of some bacterial isolates against soil borne pathogenic fungi", J. Rad. Res. Appl. Sci., vol. 4, issue 3 (A), pp. 827- 837, 2011.
Murad, H. A., R. I. Refaea, and E. M. Aly, "Utilization of UF-permeate for production of beta-galactosidase by lactic acid bacteria", Polish Journal of Microbiology, vol. 60, no. 2: -, pp. 139–144, 2011. Abstract
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2009
Gizawy, E. S., R. I. Refae, A. H. Kareem, O. M. Gomaa, and H. H. Maghrawy, "Decolorization of Congo red by Aeromonas hydrophila under microaerphilic conditions", J. Rad. Res. Appl. Sci. , vol. 2, issue 5, pp. 1027-1038, 2009.
2008
El- Kilani, R. M. M., R. I. Refae, and O. Meyer, "Enhanced bioremediation of engine oil polluted soils by nutrient application", J. Agric. Sci. Mansoura Univ., vol. 33, issue 7, pp. 5301-5313, 2008.
Refae, R. I., W. D. Saleh, and A. M. Sadic, "Microbiological studies on baby foods", J. Agric. Sci. Mansoura Univ., vol. 33, issue 9, pp. 9201-9218, 2008.
Refae, R. I., H. A. Murad, and E. M. Aly, "Production of β-galactosidase by some lactic acid bacteria grown on-permeate based medium as a waste from cheese factories", J. Agric. Sci. Mansoura Univ. , vol. 33, issue 9, pp. 6901-6912, 2008.
2007
Dawoud, I. I., R. I. Refae, and H. M. Gouda, "Effect of bacteriocines isolated from lactic acid bacteria on pathogenic bacteria", J. Agric. Sci. Zagazig Univ., vol. 22, issue 8(A), pp. 28-42, 2007.
2006
Refae, R. I., "Biodegradation of various aliphatic and aromatic hydrocarbons in sludge, compost and soil environments", BULLETIN-FACULTY OF AGRICULTURE UNIVERSITY OF CAIRO, vol. 57, no. 4: UNIVERSITY OF CAIRO, pp. 803, 2006. Abstract
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Refae, R. I., M. M. M. Ragab, T. H. Abd-El-Moity, and Y. Z. El-Sayed, "Biological control of Rhizoctonia solani damping-off of bean by Bacillus spp.", J. Agric. Sci. Mansoura Univ., vol. 31, issue 4, pp. 2321-2344, 2006.
Refae, R. I., G. Amin, H. A. Murad, and S. H. Mohamed, "Exopolysaccharide production by dairy lactic acid bacteria: Isolation, characterization of EPS-producing cultures and effect of medium supplementation and culture conditions", J. Agric. Sci. Mansoura Univ., vol. 31, issue 9, pp. 5965-5998, 2006.
2004
Higazy, A., R. I. Refae, E. A. A. El-Aal, and A. A. Awad, "BIODIVERSITY OF MARINE CYANOBACTERIA IN SODA LAKE, EGYPT I. ISOLATION AND TAXONOMIC STUDIES OF SOME UNICELLULAR AND FILAMENTOUS CULTURES", ANNALS OF AGRICULTURAL SCIENCE-CAIRO-, vol. 49: AIN SHAMS UNIVERSITY, pp. 19–40, 2004. Abstract
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Awad, A. A., R. I. Refae, E. A. A. El-Aal, and A. Higazy, "BIODIVERSITY OF MARINE CYANOBACTERIA IN SODA LAKE, EGYPT II. INFLUENCE OF SOME ENVIRONMENTAL STRESSES ON GROWTH AND N\~{} 2-FIXATION", ANNALS OF AGRICULTURAL SCIENCE-CAIRO-, vol. 49: AIN SHAMS UNIVERSITY, pp. 41–52, 2004. Abstract
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Awad, A. A., R. I. Refae, E. A. A. El-Aal, and A. Higazy, "Biological removal of dairy industrial wastes by marine cyanobacteria", J. Agric. Sci. Mansoura Univ., vol. 30, issue 11, pp. 7071-7078, 2004.
2001
Refae, R. I., K. Kloos, and O. Meyer, "Characteristics of mineral oil polluted soil and its ability of biodegradation", Annals of Agricultural Science, Ain Shams Univ.(Egypt), 2001. Abstract
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Meyer, O., R. I. Refae, and J. Baumann, "Microbial degradation of tetrachloroethylene and toluene in polluted soil", J. Agric. Sci. Mansoura Univ., vol. 26, issue 11, pp. 7005-7019, 2001.
2000
Refae, R. I., "Cadmium accumulation by microbial consortium known in Egypt as "Samakat El-Sheffa"", J. Agric. Sci. Mansoura Univ., vol. 25, issue 10, pp. 6439-6450, 2000.