Rania Darwish

Citation:

Darwish, R. K., D. I. Ramadan, A. M. Mohy, H. A. Raafat, H. E. - S. A. Youssef, and S. M. El-Kateb, "Interleukin-1 β gene polymorphisms in Egyptian patients with rheumatoid arthritis", Comparative Clinical Pathology, vol. 22, issue 1, pp. 1672-1676, 2013.

Abstract:

The present study aimed at evaluating the role of
IL-1β −511 promoter and IL-1β +3953 exon 5 gene polymorphisms
in the risk of developing rheumatoid arthritis
(RA) and its correlation to disease activity in Egyptian
patients. Thirty-two patients having RA as defined by
American College of Rheumatology and 20 healthy control
subjects were included. All were subjected to DNA analysis
for IL-1β −511and IL-1β +3953 gene polymorphisms using
PCR-RFLP technique with restriction enzymes AvaI and
TaqI, respectively. No special pattern of association could
be detected either between IL-1β −511 and +3953 gene
polymorphisms and disease susceptibility or between the
polymorphisms and the disease activity parameters represented
by disease activity score 28 (DAS 28), ESR, and
number of swollen and tender joints. Allele distribution
failed as well to detect any association with either disease
susceptibility or activity; however, a trend towards positive
association involving IL-1β −511 C allele was observed (P=
0.054). Our results suggest that IL-1β −511 and IL-1β +3953
gene polymorphisms do not influence the susceptibility to
acquire RA in our population with no relation to disease
activity. The complex role of the genetic factors in RA and
the magnitude of the effects will require further thorough
confirmations in other populations and on larger samples to
acknowledge this issue.