gene methylation status in chronic lymphocytic leukemia.

Attia, H. R. M., M. Hamed Ibrahim, S. A. H. El-Aziz, N. M. Hassan, R. A. Osman, H. A. Hagag, M. E. Yassa, A. H. Abdelrahman, I. I. Salama, and M. E. Sobeih, "gene methylation status in chronic lymphocytic leukemia.", Future science OA, vol. 6, issue 7, pp. FSO583, 2020.


BACKGROUND: We aimed to investigate gene expression pattern and to explore its methylation heterogeneity in chronic lymphocytic leukemia (CLL).

PATIENTS & METHODS: Eighty one CLL patients and 75 healthy subjects were enrolled and prognostic evaluation of patients was assessed. q-realtime PCR was performed using Applied Biosystems, TaqMan gene expression assay. gene-specific CpG methylation was investigated in real time using pyrosequencing technology.

RESULTS: was differentially expressed in CLL patients. The CpG sites-1, 2 and 3 showed significantly higher mean levels than healthy controls (p = <0.001, 0.007 and 0.009). Significant association between CpG site-1 and CLL has been detected using age-adjusted logistic regression (p < 0.001).

CONCLUSION: Hypermethylation at gene CpG sites (1,2,3) is a characteristic feature in CLL.