@article {10.1111/lam.13727,
	title = {{Evaluation of the biological activities of β-glucan isolated from Lentinula edodes}},
	journal = {Letters in Applied Microbiology},
	volume = {75},
	number = {2},
	year = {2022},
	note = {n/a},
	month = {08},
	pages = {317-329},
	abstract = {<p>{Lentinula edodes (shiitake) is an edible mushroom that contains many constituents and β-glucan is considered a major polysaccharide. This study, therefore, aimed to characterize β-glucan and evaluate its activities. Fresh fruit bodies of L. edodes were used for β-glucan extraction and followed by analyses via Fourier transform infrared, nuclear magnetic resonance and high performance liquid chromatography confirming its characteristic structure. For evaluating biological activities of β-glucan, different concentrations (0{\textperiodcentered}1{\textendash}3{\textperiodcentered}5 mg ml-1) were assessed. The optimal dose was 3{\textperiodcentered}5 mg ml-1 that showed the highest scavenging radical ability (75{\textperiodcentered}3\%) confirms antioxidant activity, strong inhibition of the peroxyl radical (80{\textperiodcentered}9\%) to inhibit lipid peroxidation, elevation the inhibition percentage of both α-amylase (73{\textperiodcentered}4\%) and α-glucosidase (70{\textperiodcentered}3\%) indicates the antidiabetic properties, and highest AFB1 reduction (88\%) which ensured the aflatoxin-detoxifying ability. In addition, antifungal activity of β-glucan was evaluated to inhibit sporulation process in Aspergillus niger and recorded with minimum inhibitory concentration of 2{\textperiodcentered}5 mg ml-1 and minimum fungicidal concentration of 3 mg ml-1. In a dose-dependent manner, higher concentration of β-glucan affects viability of tumour cells concomitant induces potent anti-cancer immune responses and inhibited the activity of topoisomerase I which are considered an important target for cancer chemotherapy. Therefore, L. edodes-β-glucan has the potential to act as a suggestive agent for antioxidant, antidiabetic, antifungal activity and aflatoxin detoxification.Significance and Impact of the Study: The isolated β-glucan was demonstrated as a potential molecule that could be used as an anti-oxidant, anti-lipid peroxidation, anti-diabetic, anti-fungal, aflatoxin-detoxifying and anti-tumour agent for biomedical applications. }</p>
},
	issn = {0266-8254},
	doi = {10.1111/lam.13727},
	url = {https://doi.org/10.1111/lam.13727},
	author = {Yehia, R.S.}
}
