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El-Zaher, A. A., and M. A. Mahrouse, "A validated spectrofluorimetric method for the determination of nifuroxazide through coumarin formation using experimental design", Chemistry Central Journal, vol. 7:90, pp. 1-12, 2013.
Elkady, E. F., R. I. El-Bagary, N. A. Farid, and N. F. Youssef, "Validated spectrofluorimetric methods for the determination of apixaban and tirofiban hydrochloride in pharmaceutical formulations", Spectrochim. Acta - Part A Mol. Biomol. Spectrosc, vol. 174, 2017.
Salama, E. N. N., M. Y. Salem, A. E. L. M. Halim, and A. L. E. Fattah, "Validated spectrophotometric and spectrofluorimetric stability-indicating methods for the determination of veralipride through charge transfer complexation", Journal of Pharmacy Research, vol. 4, issue 4, 2011.
El–Ansary, A. L., N. N. Salama, A. F. M. Attia, H. B. Hassib, and M. A. Mohamed, "Validated Spectrophotometric Methods for Determination of Escitalopram Through Study of Charge Transfer and Ion Pair Complexation", Journal of Chimica Acta, vol. 2, pp. 119-128, 2013.
Lotfy, H. M., M. A. Hegazy, S. Mowaka, and E. H. Mohamed, "Validated spectrophotometric methods for simultaneous determination of Omeprazole, Tinidazole and Doxycycline in their ternary mixture", Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy , vol. 153, pp. 321–332, 2016.
M.Lotfy, H., M. A. Hegazy, S. Mowaka, and E. H. Mohamed, "Validated spectrophotometric methods for simultaneous determination of Omeprazole, Tinidazole and Doxycycline in their ternary mixture", Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy (2016) 321–332, vol. 153 , pp. 321–332, 2016.
Al-Alamein, A. A. M., H. A. Merey, R. E. A. Kalla, and A. E. E. Gendy, "Validated Spectrophotometric Methods for Simultaneous Determination of Sulphadoxine and trimethoprim in a Veterinary Pharmaceutical Dosage Form", Research Journal of pharmacy and Technology , vol. 13, issue 11, pp. 5151-5157, 2020.
Merey, H. A., N. K. Ramadan, S. S. Diab, and A. A. Moustafa, "Validated Spectrophotometric Methods for Simultaneous Determination of Lercanidipine HCl and Enalapril Maleate in their Binary Mixture", Der Pharma Chemica, vol. 9, issue 16, pp. 33-41, 2017. sherine.pdf
Merey, H. A., N. K. Ramadan, S. S. Diab, and A. A. Moustafa, "Validated Spectrophotometric Methods for Simultaneous Determination of Lercanidipine HCl and Enalapril MALEATE in their Binary Mixture", Der Pharma Chemica, vol. 9, issue 16, pp. 33-41, 2017.
Lotfy, H. M., M. A.Hegazy, S. Mowaka, and E. H. Mohamed, "Validated spectrophotometric methods for simultaneous determination of Omeprazole, Tinidazole and Doxycycline in their ternary mixture", spectrochimica acta part A molecular and biomolecular spectroscopy, vol. 153, pp. 321-332, 2016. sca_ekram.pdf
Ekram Hany, M. Hegazy, hayam lotfy, and S. Mowaka, "Validated spectrophotometric methods for simultaneous determination of Omeprazole, Tinidazole and Doxycycline in their ternary mixture", spectrochimica acta part A molecular and biomolecular spectroscopy, vol. 153, pp. 321-332, 2016. sca_ekram2.pdf
Lotfy, H. M., M. A. Hegazy, S. Mowaka, and E. H. Mohamed, "Validated spectrophotometric methods for simultaneous determination of Omeprazole, Tinidazole and Doxycycline in their ternary mixture", Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, vol. 153: Elsevier, pp. 321-332, 2016. Abstract
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Alamein, A. A. A. M., H. A. Merey, R. E. A. Kalla, and A. E. E. Gendy, "Validated Spectrophotometric Methods for Simultaneous Determination of Sulphadoxine and trimethoprim in a Veterinary Pharmaceutical Dosage Form", Research J. Pharm. and Tech. , vol. 13, issue 11, pp. 5151-5157, 2020.
Khattab, F. I., N. K. Ramadan, M. A. Hegazy, M. A. Al-Ghobashy, and N. S. Ghoniem, "Validated Spectrophotometric Methods for Simultaneous Determination of Troxerutin and Carbazochrome in dosage form", Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy: Elsevier, 2015. Abstract

Four simple, accurate, sensitive and precise spectrophotometric methods were developed and validated for simultaneous determination of Troxerutin (TXN) and Carbazochrome (CZM) in their bulk powders, laboratory prepared mixtures and pharmaceutical dosage forms. Method A is first derivative spectrophotometry (D1) where TXN and CZM were determined at 294 and 483.5 nm, respectively. Method B is first derivative of ratio spectra (DD1) where the peak amplitude at 248 for TXN and 439 nm for CZM were used for their determination. Method C is ratio subtraction (RS); in which TXN was determined at its λmax (352 nm) in the presence of CZM which was determined by D1 at 483.5 nm. While, method D is mean centering of the ratio spectra (MCR) in which the mean centered values at 300 nm and 340.0 nm were used for the two drugs in a respective order. The two compounds were simultaneously determined in the concentration ranges of 5.00–50.00 μg mL−1 and 0.5–10.0 μg mL−1 for TXN and CZM, respectively. The methods were validated according to the ICH guidelines and the results were statistically compared to the manufacturer’s method.

Hegazy, M. A., M. A. Al-Ghobashy, N. samir Ghoneim, F. I. Khattab, and N. Khamis, "Validated spectrophotometric methods for simultaneous determination of troxerutin and carbazochrome in dosage form", spectrochimica acta part A molecular and biomolecular spectroscopy, vol. 139, pp. 206-213, 2015. nermin_sca.pdf
Khattab, F. I., N. K. Ramadan, M. A. Hegazy, M. A. Al-Ghobashy, and N. S. Ghoniem, "Validated spectrophotometric methods for simultaneous determination of troxerutin and carbazochrome in dosage form", Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, vol. 139, pp. 206 - 213, 2015. AbstractWebsite

Four simple, accurate, sensitive and precise spectrophotometric methods were developed and validated for simultaneous determination of Troxerutin (TXN) and Carbazochrome (CZM) in their bulk powders, laboratory prepared mixtures and pharmaceutical dosage forms. Method A is first derivative spectrophotometry (D1) where TXN and CZM were determined at 294 and 483.5nm, respectively. Method B is first derivative of ratio spectra (DD1) where the peak amplitude at 248 for TXN and 439nm for CZM were used for their determination. Method C is ratio subtraction (RS); in which TXN was determined at its λmax (352nm) in the presence of CZM which was determined by D1 at 483.5nm. While, method D is mean centering of the ratio spectra (MCR) in which the mean centered values at 300nm and 340.0nm were used for the two drugs in a respective order. The two compounds were simultaneously determined in the concentration ranges of 5.00–50.00μgmL−1 and 0.5–10.0μgmL−1 for TXN and CZM, respectively. The methods were validated according to the ICH guidelines and the results were statistically compared to the manufacturer’s method.

Khattab, F. I., N. K. Ramadan, M. A. Hegazy, M. A. Al-Ghobashy, and N. S. Ghoniem, "Validated spectrophotometric methods for simultaneous determination of troxerutin and carbazochrome in dosage form", Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, vol. 139: Elsevier, pp. 206-213, 2015. Abstract
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Elkady, E. F., and M. A. Mahrouse, "Validated Spectrophotometric Methods for the Simultaneous Determination of Ciprofloxacin Hydrochloride and Metronidazole in Tablets", Chem. Pharm. Bull., 2011. Abstract

A binary mixture of ciprofloxacin hydrochloride (CIP) and metronidazole (MET) was determined by five simple and accurate methods, without prior separation. In the first method, CIP was determined by second derivative spectrophotometric method (2D) by measuring the amplitude at 282 nm (zero ordinate value of MET). On the other hand, the determination of MET was based on isosbestic point technique, where the total content of the mixture was determined at 294.5 nm (isosbestic point), then the content of MET could be calculated by subtraction. The second method was first derivative ratio spectrophotometric method (1DD) where the total amplitude at 261 and 285 nm and the amplitude at 295.5 nm were selected to simultaneously determine CIP and MET in binary mixture, respectively. The third method was based on dual wavelength analysis, in which two wavelengths were selected, at which the absorbances of the other component were the same.

Mahrouse, M. A., and E. F. Elkady, "Validated spectrophotometric methods for the simultaneous determination of ciprofloxacin hydrochloride and metronidazole in tablets", Chemical and Pharmaceutical Bulletin, vol. 59, issue 12, pp. 1485—1493, 2011.
Michael, A. M., M. Shehata, A. el kadi, H. M. Lotfy, and M. Rezk, "A Validated Stability - Indicating Method for the Determination of Sumatriptan and Kinetic Study of the Degradation", Analytical Chemistry , An Indian Journal , vol. 12, issue (4), pp. 127-132, 2013.
El-Bagary, R. I., N. F. Abo-talib, and B. M. N. Eldin, "Validated Stability Indicating Assay of Gemifloxacin by Different Chromatographic and Spectrophotometric Methods of Analysis", Journal of Chemical and Pharmaceutical Research, 2011. Abstract

Five chromatographic and spectrophotometric methods have been developed for the determination of gemifloxacin (GF) in bulk powder and pharmaceutical preparations. The first method depends on RP-HPLC, separation of drug and degradation products was successfully achieved on a Hypersil BDS C18 column using mobile phase consisted of citrate buffer adjusted to 2.5 pH by citric acid: Acetonitrile (70:30, v/v) at 1 ml/min flow rate and 267 nm wavelength of detection. Another chromatographic method which achieved successful separation of drug and its degradation products depends on TLC densitometry using mobile phase consisted of chloroform: methanol: toluene: diethylamine: water (33.6:33.6:16.8:10.8:6,v/v/v/v/v) with 20?l spotting volume and 260 nm wavelength of detection. Other three simple, rapid and sensitive UV methods have been developed for GF estimation in presence of its degradation products. One method depends on the first order derivative where GF shows sharp peak at 258.6 nm.

yasmin fayez, N. Mostafa, joliana fawzy, and A. E. Aziz, "Validated Stability Indicating Chromatographic Methods for Determination of IvabradineHydrochloride in the Presence of Its Acidic Degradation Product", International Journal of Research and Reviews in Pharmacy and Applied sciences, vol. 6, issue 1, pp. 1370-1380, 2016. joliana.pdf
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