Zhang, K., Y. Xing, G. Wang, R. Shemi, M. Duan, W. L., and X. Xie,
"Ridge-furrow with film mulching practice ameliorates soil microbial metabolic activity and carbon utilization in rhizosphere soil of rapeseed (Brassica napus L.)",
Soils and Sediments, vol. 19, pp. 2764–2776, Submitted.
Shemi, R., R. Wang, E. - S. M. S. Gheith, H. A. Hussain, L. Cholidah, K. Zhang, S. Zhang, and L. Wang,
"Role of exogenous-applied salicylic acid, zinc and glycine betaine to improve drought-tolerance in wheat during reproductive growth stages",
BMC Plant Biology, vol. 21, issue 574 (2021), Submitted.
kader, A. E. M. A. E., D. S. Abdelfattah, M. M. Kamel, and H. I. H. Shousha,
Role of Serum Amyloid A and lncRNA-AF085935 as diagnostic biomarkers in serum of Hepatocellular Carcinoma, hepatitis B virus and hepatitis C virus patients. ,
, cairo, cairo university, Submitted.
AbstractBackground:
Hepatocellular carcinoma (HCC) ranks as 5th most common cancer. Chronic infection by hepatitis viruses especially HBV and HCV lead to complicated microenvironment which may lead to hepatocarcinogenesis. Lnc RNAs and serum Amyloid A (SAA) have been suggested to have role in tumorogenesis and progression. In this work we investigated the role of lnc RNA AF085935 and SAA as a diagnostic biomarkers in sera of HBV, HCV, HCC patients.
Subjects and Methods: RT-PCR technique was used to assess Lnc RNA AF085935 quantitative expression and ELISA technique was used to detect expression level of SAA at sera of 149 adult Egyptian subjects. They were divided as 35 HCC patients on top of HCV and 24 HCC patients on top of HBV, 35 patients with HBV and 35 patients with HCV and 20 healthy donors.
Results: The level of LncRNA-AF085935 and SAA were high significantly upregulated in HCC patients, HCV patients and HBV patients compared with that in normal controls (p value = <0.001). In addition, higher expressions of Lnc RNA-AF085935 and SAA were observed in HCC patients than in HCV and HBV patients. SAA showed a relatively high accuracy for HCC screening (area under the curve (AUC) = 0.927, 95 % confidence interval (CI) = 0.858-0.997) to be differentiated from normal control. LncRNA-AF085935 showed a relatively higher accuracy for HCC screening ( AUC= 0.931, 95 % CI = 0.862-1.00) than SAA (AUC = 0.74, 95 % CI = 0.587-0.894) from HBV patients.
Conclusion: Lnc RNA-AF085935 and SAA were observed with an aberrant serum level in sera of HCC, HCV and HBV patients. Lnc RNA-AF085935 and/or SAA can be used as potential biomarkers in HCC tumorogenesis.