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mohsensobhy, "Purification of Rhyzopus delmar lipase .", Egyptian J. of food sci., vol. 14, issue 1, pp. 147 – 156, 1986. Abstract
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mohsensobhy, "Purification of glucoamylase", Egyption J., . of Microbiology, vol. 12, pp. 89–99, 1977. Abstract
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mohsensobhy, "Purification of glucoamylase", Egyption J., . of Microbiology, vol. 12, pp. 89-99, 1977. Abstract
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Mohamed, M. S. M., M. S. Khalil, M. A. Tagrida, and Y. A. Mawgoud, "Purification of gelatinase from the bacteria contaminating gelatin production process", Research Journal of Pharmaceutical, Biological and Chemical Sciences, vol. 8, issue 4, pp. 914-930, 2017. 110.pdf
, "Purification of bean yellow mosaic virus.", Egypt. J. Phytopathol., vol. 18, no. (1), pp. 59–64, 1986. Abstract
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Ahmed, H., H. A. B. U. E. L. ZAHAB, and G. Alswiai, "Purification of antioxidant protein isolated from Peganum harmala and its protective effect against CCl 4 toxicity in rats.", Turkish Journal of Biology, vol. 37, no. 1, 2013. Abstract
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Abdel-Salam, A. M., K. E. M., and M. A. S. El-Kady, "Purification of an Egyptian isolate of peanut mottle virus.", Egypt, J. Phytopathol., vol. 18, no. (2), pp. 113–121, 1986. Abstract
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El-Ansary, A. L., G. A. M. El-Wahab, Eman E. Bayoumi, and E. - S. A. Nouh, "Purification of Abu-Zenima wet crude yellow cake using alkaline leaching of U(VI)", Egypt. J. Petro., vol. 27, pp. 523-530, 2018.
Albadawy, H. A., and G. A. M. Abu-El-Zahab, H.S.H. and Alswiai, "Purification of a novel antioxidant protein from Peganum harmala and its protective effect against CCl4 toxicity in rats", Turkish Journal of Biology,, vol. 37, issue 1: Turkish Journal of Biology,, pp. 39-48, 2013. Abstract
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Kord, M. A. E., E. A. Youssef, H. E. Ahmed, and E. A. Qaid, "Purification and some properties of trehalase from Triticum aestivum seeds", Proceedings of the 2nd International Conference of Physiological, Microbiological, and Ecological Plant Sciences. University of Mina, Egypt, 2012. Abstract
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El-Kady, M. A. S., G. E. - D. A. S., and A. M. Abdel-Salam, "Purification and serology of alfalfa mosaic virus in Egypt.", The 1 st Nat. Conf. of Pests and Dis. of Veg. and Field Crops in Egypt. Ismailia, Oct. 1985., 1985. Abstract
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Abdel-Salam, A. M., E. - K. M., and S. A. A., "Purification and serology of a local isolate of watermelon mosaic virus 2 (WMV-2) in Egypt: A modified technique for purification", The 4th Conf. Pests and Veg. and Fruit Dis. in Egypt: Oct. 1991., Ismailia, Egypt, 1991. Abstract
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El-Kady, M. A. S., O. - H. M. El-Banna, E. A. Salama, and S. N. Zein, "Purification and Serological Studies on Barley Stripe Mosaic Hordevirus", Egyptian J. Virol, vol. 1, pp. 149-156, 2004. Abstract
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Abdel-Salam, A. M., "Purification and serological studies on a local isolate of bean common mosaic virus in Egypt", Proc. 7 the Conf. of Microbiol., Cairo, Egypt. pp 370-378. Nov. 19-20, 1989., 1989. Abstract
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Abdel-Salam, A. M., K. M. M. A. H. M., and M. Ibrahim, "Purification and serologic studies on an Egyptian isolate of potato virus Y (PVY) isolated from pepper plants", Egypt. J. Phytopathol., vol. 21, pp. 60–64, 1989. Abstract
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Baccanari, D., A. Phillips, S. Smith, D. Sinski, and J. Burchall, "Purification and properties of Escherichia coli dihydrofolate reductase.", Biochemistry, vol. 14, issue 24, pp. 5267-73, 1975 Dec 2. Abstract

Dihydrofolate reductase has been purified 40-fold to apparent homogeneity from a trimethoprim-resistant strain of Escherichia coli (RT 500) using a procedure that includes methotrexate affinity column chromatography. Determinations of the molecular weight of the enzyme based on its amino acid composition, sedimentation velocity, and sodium dodecyl sulfate gel electrophoresis gave values of 17680, 17470 and 18300, respectively. An aggregated form of the enzyme with a low specific activity can be separated from the monomer by gel filtration; treatment of the aggregate with mercaptoethanol or dithiothreitol results in an increase in enzymic activity and a regeneration of the monomer. Also, multiple molecular forms of the monomer have been detected by polyacrylamide gel electrophoresis. The unresolved enzyme exhibits two pH optima (pH 4.5 and pH 7.0) with dihydrofolate as a substrate. Highest activities are observed in buffers containing large organic cations. In 100 mM imidazolium chloride (pH 7), the specific activity is 47 mumol of dihydrofolate reduced per min per mg at 30 degrees. Folic acid also serves as a substrate with a single pH optimum of pH 4.5. At this pH the Km for folate is 16 muM, and the Vmax is 1/1000 of the rate observed with dihydrofolate as the substrate. Monovalent cations (Na+, K+, Rb+, and Cs+) inhibit dihydrofolate reductase; at a given ionic strength the degree of inhibition is a function of the ionic radius of the cation. Divalent cations are more potent inhibitors; the I50 of BaCl2 is 250 muM, as compared to 125 mM for KCl. Anions neither inhibit nor activate the enzyme.

Youssef, M. K., Q. Wang, S. W. Cui, and S. Barbut, "Purification and partial physicochemical characteristics of protein free fenugreek gums", Food Hydrocolloids, vol. 23, no. 8: Elsevier, pp. 2049–2053, 2009. Abstract

Crude fenugreek gum (3.74% protein) was purified by dissolving in aqueous solvent and centrifugation to remove impurities which yielded a purified gum fraction containing 1.10% protein residue. Further purification of the gum was achieved by treating the gum solution with phenol to obtain protein free fenugreek gum (0.16% protein residue). The three types of fenugreek gums were evaluated for: molecular weight, surface activity and rheological performance. Surface and interfacial tension, measured by a Du Nouy ring, indicated that the removal of protein in the gum significantly reduced its surface activity. However, the crude fenugreek gum exhibited lower intrinsic viscosity and radius of gyration compared to the purified and protein free fenugreek gums. It was found that both protein residue and gum concentration affected the elastic modulus (G′), viscous modulus (G″), and complex viscosity (η∗).

Mohy-Eldin, A., Z. Kamel, and N. Hossam, "Purification and identification of surface active amphiphilic candidates produced by Geotrichum candidum MK880487 possessing antifungal property", Journal of Dispersion Science and Technology, vol. 42, issue 7, pp. 1082-98, 2021.
Elshafei, A. M., M. S. Foda, A. Aboul‐Enein, A. S. Afify, and N. H. Ali, "Purification and enzymatic properties of α‐galactosidase from Penicillium janthinellum", Acta biotechnologica, vol. 13, issue 4: Wiley Online Library, pp. 351-359, 1993. Abstract
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Seufi, A. M., G. M. El Bassiony, and S. S. Ibrahim, "Purification and Characterization of Two Antimicrobial Peptides from Bacterial-challenged Haemolymph of Bombyx mori Larva", Egypt. Acad. J. biolog. Sci., vol. 2, issue 2, pp. 231-243, 2009. 23.pdf
Kord, M., E. Youssef, H. Ahmed, and E. Qaid, "Purification and characterization of trehalase from seeds of chickpea (< i> Cicer arietinum L.)", Turkish Journal of Biology, vol. 37, no. 6: The Scientific and Technological Research Council of Turkey, pp. 661–669, 2013. Abstract
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Kandil, O. M., O. A. Mahdy, S. K. Abou-El-Doubal, and W. M. Mousa, "Purification and characterization of three larval taeniid antigens by gel filtration. ", Vet. Med. J. Giza, vol. Vol. 52.(4), pp. 449-456, 2004.
Hafez, R. M., T. M. Adel-Rahman, N. N. Abed, E. E. Mostafa, and A. Atef, "Purification and characterization of the virulent factor lipase extracted from Candida catenulate.", Plant Archives , vol. 20, issue 2, pp. 7069-7079, 2020. 7069-7079_6622.pdf
, "Purification and characterization of peroxidase from date palm (Phoenix dactylifera L.) callus cv. Barhee", J. Biol. Chem. Environ. Sci., vol. 13, issue 3, pp. 285-297, 2018. Abstract
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