Spotting the scope on triple-negative breast cancer (TNBC) as the most aggressive type of breast cancer, with no targeted therapeutic options. TNBC is often characterized by having defects in DNA repair pathway making this cancer a rational target for the Doxorubicin as a chemotherapeutic agent, and for the synthetic lethality of olaparib as an inhibitory target agent of the alternative DNA repair pathway (poly ADP-ribose polymerase “PARP” inhibitor). The present study aims to evaluate the value of miRNAs-181a/b as potential biomarkers in predicting the response of TNBC to therapy. MiRNAs-181a/b expression was knocked down via transfection with anti-miRNAs-181a/b into MDA-MB-231 cell line using HiPerFect transfection reagent, the transfected and untransfected cells were subjected to doxorubicin and olaparib separately. Essential proteins involved in apoptosis and cell proliferation including Caspase-8, Bcl-2, and Ki-67 has been detected. Further, the expression level of ataxia telangiectasia mutated (ATM) was determined as a functional target of miRNAs-181a/b. A significant decrease in Caspase-8 activity, and Bcl-2, and a significant increase in cell survival, cell proliferation, and ATM protein were observed upon suppression of miRNAs-181a/b by their inhibitors followed by treatment with eithr doxorubicin or olaparib for TNBC cell line (MDA-MB-231 cells). Collectively, our data confirmed that miRNA-181a and miRNA-181b play a critical role for detecting the sensitivity of TNBC cells to treatment. As well as miRNAs-181a/b could be used as predictive biomarkers for response to therapy.

The objective of this study was to use the data of growth patterns of local
chickens accompanied with their molecular data to develop a strategy for markerassisted
selection for growth. Four lines were used and have been derived from a
naturally heat resistant local breed in Egypt. They were a homozygous normallyfeathered
selected line (CE1), homozygous naked-neck selected line (CE3) and
their corresponding control lines (CE2 and CE4). Lines CE1 and CE3 have been
selected for high 6-wk BW for five generations. Three generations were obtained
for this study. Lines CE1 and CE3 were significantly heavier at 6 weeks of age
than their corresponding control lines CE2 and CE4 by 24.15 and 27.07% in the
base generation and by 51.4 and 34.5% in the second selected generation. The
differences reached to 57.1 and 62.8% at 18 weeks of age in the base generation
and 49.3 and 28.7% in the second selected generation. Line CE1 was significantly
heavier than line CE3 throughout the growing period. The total number of alleles
per locus averaged 7.78 alleles. Polymorphism percentage averaged 50.1 and
55.1% in lines CE1 and CE3 versus 38.6 and 49.6% in lines CE2 and CE4.
Percentage of unique alleles averaged 4.3 and 4.6% in lines CE1 and CE3. Many
polymorphic allelic bands were differed in their frequencies between high and low
performed families in lines CE1 and CE3. A total of 34 polymorphic alleles in 19
loci were flown over generations and the flow trends were different in different
lines and families within lines. Principle component analysis was applied to the
data of both lines and two main components were found and three canonical
correlations were obtained. Four and five bands were persistently concomitant
with the highly performed families in lines CE1 and CE3, respectively. These
bands would have brought 6-wk BW into higher levels of performance by 25.1
and 16.6% in the second generation if they would have been considered in the
selection for 6-wk BW.