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2024
El-Monshed, A. H., L. M. Khonji, M. Altheeb, M. T. E. - M. Saad, M. A. Elsheikh, A. Loutfy, A. S. Ali, H. E. El-Gazar, S. M. Fayed, and M. A. Zoromba, "Does a program-based cognitive behavioral therapy affect insomnia and depression in menopausal women? A randomized controlled trial.", Worldviews on evidence-based nursing, vol. 21, issue 2, pp. 202-215, 2024. Abstract

BACKGROUND: Menopausal women often complain of a range of physical and psychological symptoms known as menopausal syndrome. These symptoms are associated with fluctuating hormone levels, sleep disturbances, and mood swings.

AIM: This study aimed to examine the efficacy of a program-based cognitive behavioral group therapy (CBT) for insomnia and depression among women experiencing menopause.

METHODS: A randomized controlled trial of 88 women experiencing menopause was conducted in Egypt from June to September 2022 in outpatient clinics at Mansoura University Hospitals in Egypt. Participants were randomly assigned to a control group (45 women) and an intervention group (43 women). The intervention group received 7 weeks of CBT sessions. Insomnia Severity Index (ISI), Pittsburgh Sleep Quality Index (PSQI), and Beck Depression Inventory 2nd Edition (BDI-II) were administered before and after the intervention.

RESULTS: In the intervention group, there was a significant difference in scores of the subdomains of PSQI, including sleep efficiency, daytime dysfunction, subjective sleep quality, and sleep disturbance (t = 8.911, 11.77, 7.638, and 11.054, respectively), while no significant difference in domains of using sleep medication, sleep duration, and sleep latency. Significant improvements were observed between pre-and-post-intervention in the intervention group for the total scores of PSQI, ISI, and BDII-II (t = 12.711, 16.272, and 12.384, respectively), indicating a large effect size for the three studied variables (r = .81, .87, .8, respectively).

LINKING EVIDENCE TO ACTION: This study demonstrated the efficacy of group CBT for lowering insomnia and depression in women experiencing menopause. Thus, results indicated the need of considering prompt and appropriate interventions such as CBT as a safe treatment option to prevent the aggravation of sleep and emotional problems for menopausal women.

TRIAL REGISTRATION: ClinicalTrials.gov Identifier: NCT05920460.

Rania Mostafa, and M. S. Temerak, "Does consumer empowerment enhance brand page stickiness? The role of brand page experience and brand love", Journal of Research in Interactive Marketing, 2024.
Laimoud, M., P. Machado, M. G. Lo, M. J. Maghirang, and E. Hakami, "DOES LACTATE CLEARANCE PROGNOSTICATE PATIENTS WITH POSTCARDIOTOMY VENOARTERIAL EXTRACORPOREAL MEMBRANE OXYGENATION?", Journal of American College of Cardiology , vol. 83, 2024.
Benaya, A. M., M. S. Hassan, M. H. Ismail, and T. Landolsi, "Double-Faced Active Intelligent Reflecting Surfaces-Assisted Symbiotic Radio Communications", IEEE Open Journal of Vehicular Technology, vol. 5, pp. 577 – 591, 2024.
El-Beltagi, H. S., A. A. Halema, Z. M. Almutairi, H. H. Almutairi, N. A. G. W. A. I. ELARABI, A. A. Abdelhadi, A. R. Henawy, and H. A. R. Abdelhaleem, "Draft genome analysis for Enterobacter kobei, a promising lead bioremediation bacterium", Front. Bioeng. Biotechnol., vol. 11, pp. 1335854., 2024.
El-Beltagi, H. S., A. A. Halema, Z. M. Almutairi, H. H. Almutairi, N. A. G. W. A. I. ELARABI, A. A. Abdelhadi, A. R. Henawy, and H. A. R. Abdelhaleem, Draft genome analysis for Enterobacter kobei, a promising lead bioremediation bacterium, , 2024.
Mohamed I. El Sabry, A. Hassan, T.A. Ebeid, and F. Abou-Hashim, "Drinking magnetized water alters blood constituents, and structure of spleen and kidney in rabbitts", Online J. Anim. Feed Res., vol. 14, issue 2, pp. 86-94, 2024.
Abou-Hashim, F., "Drinking magnetized water alters blood constituents, and structure of ‎spleen and kidney in rabbits", Online Journal of Animal and Feed Research, vol. 14, issue 2, pp. 86-94, 2024.
Zeng, H., B. Wu, A. Elnashar, and Z. Fu, "Dryland Dynamics in the Mediterranean Region", Dryland Social-Ecological Systems in Changing Environments: Springer, 2024. Abstract

Mediterranean drylands are rich in biodiversity and play an important role in global ecosystem sustainable management. This study summarizes the characteristics, dynamic change, and change drivers of Mediterranean drylands. The drylands showed strong spatial heterogeneity, hyperarid and arid regions were dominant in North Africa and West Asia, and semiarid and dry subhumid regions were widely distributed in European countries. Mediterranean dryland is experiencing a warming trend that would become stronger under representative concentration pathways (RCP) 4.5 and 8.5, which would increase the risk of land degradation and desertification. Arid North Africa and West Asia faced rapid population growth that put considerable pressure on food supply and water consumption. The conflicts among land, water, food, and the ecosystem intensified under the warming trend. The significant expansion of cropland and urbanization was widely observed in arid areas, such as Egypt, while the rotation of land reclamation, degradation, abandonment, and reclamation was observed in arid areas and caused large-scale cross-border migration. The Mediterranean region had low food self-sufficiency due to a booming population, and the crop structure of cash crops was dominant. The expansion of cropland also significantly increased the water consumption in the arid area of the Mediterranean region, and water consumption increased by 684.54 × 106 m3 from 2000 to 2020 in Egypt. More robust models and fine spatial resolution data should be developed for the sustainable development of Mediterranean drylands.

Kadić-Maglajlić, S., C. R. Lages, and M. S. Temerak, "Dual perspective on the role of xenophobia in service sabotage", Tourism Management , vol. 101, pp. 104831, 2024.
Tantawy, M., and H. I. Abdel-Gawad, "Dynamics of molecules in torsional DNA exposed to microwave and possible impact on its deformation: stability analysis", The European Physical Journal Plus, vol. 139, no. 4: Springer Berlin Heidelberg, pp. 1–17, 2024. Abstract
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2023
Shaalan, O., "DIAGNOdent versus International Caries Detection and Assessment System in detection of incipient carious lesions: A diagnostic accuracy study", Journal of Conservative Dentistry, vol. 26, issue 2, pp. 199-206, March 1, 2023. Abstractjconservdent_2023_26_2_199_371798.pdfWebsite

Aim: The present investigation aimed at assessing the diagnostic accuracy of DIAGNOdent compared to the International Caries Detection and Assessment System-II (ICDAS-II) in the detection of facial, smooth surface noncavitated carious lesions.Settings and Design: Sixty patients were enrolled in the current study according to the eligibility criteria. There were 161 teeth with noncavitated, white spot carious lesions and 32 sound teeth.Materials and Methods: Before examination, teeth were cleaned and polished and all patients were assessed under standardized operating conditions: preset dental unit position, operating light, and prolonged air drying (approximately 5 s). All teeth were assessed by two calibrated examiners individually without any contact using ICDAS-II and DIAGNOdent.Statistical Analysis: The diagnostic accuracy of DIAGNOdent device was determined, including sensitivity, specificity, overall accuracy, positive and negative predictive values, and receiver operating characteristic curve analysis. Chi-square test was conducted to compare the distribution of ICDAS-II and DIAGNOdent scores. Inter-observer agreement between assessors was evaluated using Cohen's kappa test.Results: In the current study, DIAGNOdent had an overall accuracy of 84.45% with sensitivity and specificity of 87.58% and 96.87%, respectively, and +PV and −PV of 97.7% and 83.9%, respectively, when score 0 represented sound tooth surface, while scores 1 and 2 were considered clinically noncavitated carious lesions. Moreover, when only ICDAS score 1 was considered representing first visual change in enamel, DIAGNOdent had an accuracy of 74.15% with sensitivity and specificity of 83.53% and 90.62%, respectively, and +PV and −PV of 93% and 78.6%, respectively. In the present study, when only ICDAS score 2 was considered representing distinct visual change in enamel, DIAGNOdent had an accuracy of 100% with sensitivity and specificity of 100% and 100%, respectively, and +PV and −PV of 100% and 100%, respectively.Conclusions: The overall performance of DIAGNOdent was equivalent to the visual inspection using ICDAS-II. DIAGNOdent might be considered a useful adjunctive device for detection and monitoring development of noncavitated carious lesions on facial smooth surfaces.

Mostafa, H. I. A., "Detection of bacteriorhodopsin trimeric rotation at thermal phase transitions of purple membrane in suspension", Biophysical chemistry, vol. 300, pp. 107074, July, 2023. AbstractWebsite

Bacteriorhodopsin (bR) of purple membrane (PM) is a retinal protein that forms aggregates in the form of trimers constituting, together with archaeal lipids, the crystalline structure of PM. The rotary motion of bR inside PM may be pertinent in understanding the essence of the crystalline lattice. An attempt has been made to determine the rotation of bR trimers which has been found to be detected solely at thermal phase transitions of PM, namely lipid, crystalline lattice and protein melting phase transitions. The temperature dependences of dielectric versus electronic absorption spectra of bR have been determined. The results suggest that the rotation of bR trimers, together with concomitant bending of PM, are most likely brought by structural changes in bR which might be driven by retinal isomerization and mediated by lipid. The rupturing of the lipid-protein contact might consequently lead to rotation of trimers associated with bending, curling or vesicle formation of PM. So the retinal reorientation may underlie the concomitant rotation of trimers. Most importantly, rotation of trimers might play a role, in terms of the essence of the crystalline lattice, in the functional activity of bR and may serve physiological relevance.

Kassem, E., S. A. Khaled, M. Abdelkarim, A. G. El-Hamalawy, and M. Fahmy, "Does minimally invasive percutaneous transilial internal fixator became an effective option for sacral fractures? A prospective study with novel implantation technique", European Journal of Trauma and Emergency Surgery: Springer Science and Business Media {LLC}, jan, 2023. AbstractWebsite
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Shorim, N., M. El-Ramly, and H. Bayomi, "Design Pattern Recommendation Using Doc2vec", Eleventh International Conference on Intelligent Computing and Information Systems (ICICIS), Egypt, 21-23 November, 2023.
Al-Zahrani, H. S., T. A. A. Moussa, H. Alsamadany, R. M. Hafez, and M. P. Fuller, De Novo Transcriptome Analysis of Solanum lycopersicum cv. Super Strain B under Drought Stress, , vol. 13, issue 9: MDPI, pp. 2360, 2023. Abstract
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Hassan, S. A., A. M. Mahmoud, M. A. Kamel, S. S. Abbas, and A. M. Michael, Design of Solid-contact Ion-selective Electrode with Multiwall-carbonNanotubes for Assay of Sulfacetamide in Rabbit Aqueous Humour , , vol. 19, issue 4, pp. 320 - 329, 2023. Abstract
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Cheng, L., J. - R. Liu, J. - M. Liu, D. Guo, F. Deng, Q. Bian, H. Zhang, X. Han, A. S. Ali, W. - H. Zhang, et al., "Design, synthesis, antifungal activity and molecular docking of ring-opened pimprinine derivative containing (thio)amide structure", Pest Management Science, vol. 79, issue 6, pp. 2220 - 2229, 2023. AbstractWebsite
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Liu, J. - R., Y. Gao, B. Jin, D. Guo, F. Deng, Q. Bian, H. - F. Zhang, X. - Y. Han, A. S. Ali, M. - Z. Zhang, et al., "Design, Synthesis, Antifungal Activity, and Molecular Docking of Streptochlorin Derivatives Containing the Nitrile Group", Marine Drugs, vol. 21, issue 2, 2023. AbstractWebsite
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Abdelgwad, M., W. Tawfik, A. Zedan, and O. Radwan, Determination of Saturated and Unsaturated Hydrocarbons in Petroleum Oil Pesticides using FTIR Spectroscopy, , pp. - , 2023. AbstractWebsite

Petroleum oil pesticides are used as acaricides, insecticides, and herbicides. Oils can cause phytotoxicity if oils contain unsaturated hydrocarbons. Traditional chemical methods to determine unsulfonated residue (UR), such as the ASTM D483 and MT (57), depending on sulfuric acid 98% reagent. The present study introduces a new optical spectroscopic method using FTIR to determine unsaturated Petroleum oil pesticides. The integral intensities of the bands at about 1640 to 1643 cm−1 correspond to ν(C=C). In alkynes, (C≡C 2140-2100 cm-1). Three technical petroleum oil samples are collected from three different companies in Egypt. (El-Mostakble for Chemicals Co.(S1), Kafr El-Zayate for Pesticides and Chemicals (KZ)(S2), and Misr Petroleum Refining Co(S3). FTIR results showed peaks demonstrated more absorbance while transmittance decreased in all samples at 1643 to 1640 cm-1 and 2121 cm-1 when adding 1-octene 99% and 1-Heptyne 99% to the oil of all samples. Therefore, FTIR can detect and determine unsaturated technical petroleum oils. Moreover, the method of FTIR has advantages in preventing exposure to the toxicity of acids and can differentiate between carbon double bond and triple bond

Abdelmoneim, T. K., M. S. M. Mohamed, I. A. Abdelhamid, S. F. M. Wahdan, and M. A. M. Atia, "Development of rapid and precise approach for quantification of bacterial taxa correlated with soil health", Frontiers in Microbiology, vol. 13, 2023. Abstract

The structure and dynamic of soil bacterial community play a crucial role in soil health and plant productivity. However, there is a gap in studying the un−/or reclaimed soil bacteriome and its impact on future plant performance. The 16S metagenomic analysis is expensive and utilize sophisticated pipelines, making it unfavorable for researchers. Here, we aim to perform (1) in silico and in vitro validation of taxon-specific qPCR primer-panel in the detection of the beneficial soil bacterial community, to ensure its specificity and precision, and (2) multidimensional analysis of three soils/locations in Egypt (‘Q’, ‘B’, and ‘G’ soils) in terms of their physicochemical properties, bacteriome composition, and wheat productivity as a model crop. The in silico results disclosed that almost all tested primers showed high specificity and precision toward the target taxa. Among 17 measured soil properties, the electrical conductivity (EC) value (up to 5 dS/m) of ‘Q’ soil provided an efficient indicator for soil health among the tested soils. The 16S NGS analysis showed that the soil bacteriome significantly drives future plant performance, especially the abundance of Proteobacteria and Actinobacteria as key indicators. The functional prediction analysis results disclosed a high percentage of N-fixing bacterial taxa in ‘Q’ soil compared to other soils, which reflects their positive impact on wheat productivity. The taxon-specific qPCR primer-panel results revealed a precise quantification of the targeted taxa compared to the 16S NGS analysis. Moreover, 12 agro-morphological parameters were determined for grown wheat plants, and their results showed a high yield in the ‘Q’ soil compared to other soils; this could be attributed to the increased abundance of Proteobacteria and Actinobacteria, high enrichment in nutrients (N and K), or increased EC/nutrient availability. Ultimately, the potential use of a taxon-specific qPCR primer-panel as an alternative approach to NGS provides a cheaper, user-friendly setup with high accuracy. Copyright © 2023 Abdelmoneim, Mohamed, Abdelhamid, Wahdan and Atia.

El-Adawy, M. M., M. M. Attia, M. Y. Elgendy, M. Abdelsalam, and A. Fadel, Development of silver nano-based indirect ELISA and Dot-ELISA methods for serological diagnosis of a bacterial fish pathogen Aeromonas veronii, , vol. 211, pp. 106782, 2023. AbstractWebsite

Rapid and accurate detection of bacterial pathogens is critical in controlling disease outbreaks affecting farmed fish. The present study aimed to develop a novel serological diagnostic approach using nano‑silver based Enzyme-linked immunosorbent assay (ELISA) for speedy detection of Aeromonas veronii infections in Nile tilapia. A. veronii isolates used in ELISA assays were recovered from moribund Nile tilapia during a disease outbreak in a private fish farm in Egypt. A. veronii isolates were identified based on alignment analysis of the gyrB and 16S rRNA gene sequences. A. veronii antisera used in ELISA assays were prepared in tilapia, and the bacterial antigens were formalin-killed. The cut-off values were 0.46 and 0.48 in traditional and nano-based ELISA. There were no cross-reactions with bacterial isolates (Aeromonas hydrophila, Aeromonas caviae, Aeromonas sobria, Pseudomonas fluorescens, and Vibrio vulnificus). The lowest antigen concentration that produced positive results after checkerboard titration in indirect-ELISA (i-ELISA) and dot ELISA was 15 μg and 250 ng of prepared antigen, respectively. Nano-ELISA and nano-based dot-ELISA antigen concentration was 10 μg and 100 ng, respectively. Sera concentration was 1:100 in indirect-ELISA and dot-ELISA, while it was 1:50 in nano-based ELISA and nano dot-ELISA. The i-ELISA successfully detected anti-Aeromonas IgG antibodies with 83.33% sensitivity and 66.67% specificity, while in the dot-ELISA, the sensitivity and specificity were 83.33% and 100%, respectively. Nano dot-ELISA had 100% sensitivity, specificity, and accuracy. Nano dot-ELISA assays have higher specificity, sensitivity, and accuracy than traditional ELISAs in detecting A. veronii. Further studies are needed to develop a rapid test kit for on-site field diagnosis.

, Development of SSR Markers to Characterize Potato (Solanum tuberosum L.) Somaclones with Improved Starch Accumulation, , vol. 63, issue 3, pp. 1173 - 1185, 2023. AbstractWebsite

This study investigated potato tubers' starch and sugar content from different callus-sourced clones compared to the original cultivar Lady Rosetta. The clones were categorized based on their starch content, with some clones having similar starch content to Lady Rosetta, some with lower starch content, and others with higher starch content. The tubers were grown in a specific mixture of sand, peat moss, perlite, vermiculite, and foam for four months, after which their dry matter, starch, and sugar content were analyzed. The sugar content was determined using ethanol extraction, while the starch content was measured using the anthrone method. The results unveiled significant differences in starch and sugar contents among the tested potato clones. While soluble sugar content showed no significant correlation with starch content, a significant positive correlation was found between dry matter content and starch content. The dry matter content varied among the clones, with some showing significantly higher values than Lady Rosetta. SSR-PCR analysis was performed using six SSR primers to assess genetic diversity among potato clones. The analysis generated distinct and reproducible banding patterns, with 68 bands, of which 62 were polymorphic. The primers amplified unique bands specific to certain clones, and the polymorphic information content (PIC) was calculated for each marker. The genetic relationships among the clones were illustrated using a clustering dendrogram based on Jaccard's coefficient. In conclusion, the utilized SSR markers effectively identified the starch content in potato tubers clones originating from callus, thereby contributing to advancements in potato breeding and crop improvement endeavors.

Adly, W. M. R. M., H. S. Abdelkader, M. A. Mohamed, M. E. EL-Denary, E. S. T. Sayed, and A. S. Fouad, Development of SSR Markers to Characterize Potato (Solanum tuberosum L.) Somaclones with Improved Starch Accumulation, , vol. 63, issue 3, pp. 1173 - 1185, 2023. AbstractWebsite

This study investigated potato tubers' starch and sugar content from different callus-sourced clones compared to the original cultivar Lady Rosetta. The clones were categorized based on their starch content, with some clones having similar starch content to Lady Rosetta, some with lower starch content, and others with higher starch content. The tubers were grown in a specific mixture of sand, peat moss, perlite, vermiculite, and foam for four months, after which their dry matter, starch, and sugar content were analyzed. The sugar content was determined using ethanol extraction, while the starch content was measured using the anthrone method. The results unveiled significant differences in starch and sugar contents among the tested potato clones. While soluble sugar content showed no significant correlation with starch content, a significant positive correlation was found between dry matter content and starch content. The dry matter content varied among the clones, with some showing significantly higher values than Lady Rosetta. SSR-PCR analysis was performed using six SSR primers to assess genetic diversity among potato clones. The analysis generated distinct and reproducible banding patterns, with 68 bands, of which 62 were polymorphic. The primers amplified unique bands specific to certain clones, and the polymorphic information content (PIC) was calculated for each marker. The genetic relationships among the clones were illustrated using a clustering dendrogram based on Jaccard's coefficient. In conclusion, the utilized SSR markers effectively identified the starch content in potato tubers clones originating from callus, thereby contributing to advancements in potato breeding and crop improvement endeavors.