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2022
Rudrapal, M., A. P. Kothawade, S. M. Ezzat, and C. Egbuna, "Bioanalysis: methods, techniques, and applications", Analytical Techniques in Biosciences: Elsevier, 2022.
Khalil, N. M., H. M. Ali, and A. E. Ibrahim, "Biochemical Activity of Propolis Alcoholic Extracts against Fusarium oxysporum hm89", Egyptian Journal of Botany, vol. 62, issue 1, pp. 197-212 , 2022.
Abd-Allah, E. R., A. - E. El-Ghareeb, O. S. Hafez, and H. A. A. El-Rahman, "Biochemical and histopathological evaluations of thiamethoxam on the male reproductive system", Egyptian Journal of Chemistry, vol. 65, issue 11, pp. 677 - 689, 2022. 5-_olfat_paper.pdf
Hoter, A., and H. Y. Naim, "Biochemical Characterization of SARS-CoV-2 Spike RBD Mutations and Their Impact on ACE2 Receptor Binding.", Frontiers in molecular biosciences, vol. 9, pp. 893843, 2022. Abstract

Infection of mammalian cells by SARS-CoV-2 coronavirus requires primary interaction between the receptor binding domain (RBD) of the viral spike protein and the host cell surface receptor angiotensin-converting enzyme 2 (ACE2) glycoprotein. Several mutations in the RBD of SARS-CoV-2 spike protein have been reported for several variants and resulted in wide spread of the COVID pandemic. For instance, the double mutations L452R and E484Q present in the Indian B.1.617 variant have been suggested to cause evasion of the host immune response. The common RBD mutations N501Y and E484K were found to enhance the interaction with the ACE2 receptor. In the current study, we analyzed the biosynthesis and secretion of the RBD double mutants L452R and E484Q in comparison to the wild-type RBD and the individual mutations N501 and E484K in mammalian cells. Moreover, we evaluated the interaction of these variants with ACE2 by means of expression of the S protein and co-immunoprecipitation with ACE2. Our results revealed that the double RBD mutations L452R and E484Q resulted in a higher expression level and secretion of spike S1 protein than other mutations. In addition, an increased interaction of these mutant forms with ACE2 in Calu3 cells was observed. Altogether, our findings highlight the impact of continuous S1 mutations on the pathogenicity of SARS-CoV-2 and provide further biochemical evidence for the dominance and high transmissibility of the double Indian mutations.

Moustafa, A. A., T. T. El-Idreesy, and R. R. Mohamed, "Biodegradable Cross-Linked Carboxymethyl Kappa Carrageenan/Poly(Ethylene Glycol)/MMT Nanocomposites Synthesis and their Usage in Water Treatment", EC Microbiology , vol. 18, issue 3, pp. 59-76, 2022.
Kamal, I. M., N. F. Abdeltawab, Y. M. Ragab, M. A. Farag, and M. A. Ramadan, "Biodegradation, Decolorization, and Detoxification of Di-Azo Dye Direct Red 81 by Halotolerant, Alkali-Thermo-Tolerant Bacterial Mixed Cultures", Microorganisms, vol. 10, issue 5, pp. 994, 2022.
Kamal, I. M., N. F. Abdeltawab, Y. M. Ragab, M. A. Farag, and M. A. Ramadan, "Biodegradation, Decolorization, and Detoxification of Di-Azo Dye Direct Red 81 by Halotolerant, Alkali-Thermo-Tolerant Bacterial Mixed Cultures.", Microorganisms, vol. 10, issue 5, 2022. Abstract

Azo dyes impact the environment and deserve attention due to their widespread use in textile and tanning industries and challenging degradation. The high temperature, pH, and salinity used in these industries render industrial effluent decolorization and detoxification a challenging process. An enrichment technique was employed to screen for cost-effective biodegraders of Direct Red 81 (DR81) as a model for diazo dye recalcitrant to degradation. Our results showed that three mixed bacterial cultures achieved ≥80% decolorization within 8 h of 40 mg/L dye in a minimal salt medium with 0.1% yeast extract (MSM-Y) and real wastewater. Moreover, these mixed cultures showed ≥70% decolorization within 24 h when challenged with dye up to 600 mg/L in real wastewater and tolerated temperatures up to 60 °C, pH 10, and 5% salinity in MSM-Y. Azoreductase was the main contributor to DR81 decolorization based on crude oxidative and reductive enzymatic activity of cell-free supernatants and was stable at a wide range of pH and temperatures. Molecular identification of azoreductase genes suggested multiple genes per mixed culture with a possible novel azoreductase gene. Metabolite analysis using hyphenated techniques suggested two reductive pathways for DR81 biodegradation involving symmetric and asymmetric azo-bond cleavage. The DR81 metabolites were non-toxic to nauplii and seeds. This study provided evidence for DR81 degradation using robust stress-tolerant mixed cultures with potential use in azo dye wastewater treatment.

Atwa, D.M., I. S., A. B. Stani, C, B. y. Grenni, P., and W. Soliman, "Biodeterioration Assessment of a Unique Old Pharaonic Kingdom Wooden Statue Using Advanced Diagnostic Techniques", Applied Sciences, vol. 12, 2022.
Fetyan, N. A. H., A. E. -khairB. El-Sayed, F. M. Ibrahim, Y. A. Attia, and M. W. Sadik, "Bioethanol production from defatted biomass of Nannochloropsis oculata microalgae grown under mixotrophic conditions", Environmental Science and Pollution Research, vol. 29, issue 2, pp. 2588–2597, 2022. bioethanol_production_from_defatted_biomass_of_nan_1.pdf
Attia, Y. A., E. M. Abdelsalam, S. Saeed, M. S. M. Mohamed, Y. M. A. Mohamed, S. H. Abdel‑Hafez, and M. Samer, "Bioethanol production from potato peels using Saccharomyces cerevisiae treated with ZnO and ZnO/g-C3N4 nanomaterials", Egyptian Journal of Chemistry, vol. DOI: 10.21608/ejchem.2022.118978.5351, 2022.
Attia, Y. A., E. M. Abdelsalam, S. Saeed, M. S. M. Mohamed, and M. Samer, "Bioethanol production from potato peels using Saccharomyces cerevisiae treated with ZnO and ZnO/g-C3N4 nanomaterials", Egyptian Journal of chemistry, vol. 65, issue 13, pp. 309 – 315, 2022. ejchem_volume_65_issue_13_pages_309-315_1.pdf
Abuhlega, T. A., and M. R. Ali, "Biogenic amines in fish: Prevention and reduction", Journal of Food Processing and Preservation , vol. 46, issue e16883, pp. 1-15, 2022. food_processing_preservation_-_2022_-_abuhlega_-_biogenic_amines_in_fish__prevention_and_reduction.pdf
Elbialy, N. S., S. F. Aboushoushah, and N. Mohamed, "Bioinspired synthesis of protein/polysaccharide-decorated folate as a nanocarrier of curcumin to potentiate cancer therapy", International journal of pharmaceutics, vol. 613, pp. 121420, 2022.