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Fathy, M., M. Hamed, O. Youssif, N. Fawzy, and W. Ashour, " Association Between Environmental Tobacco Smoke Exposure and Lung Cancer Susceptibility: Modification by Antioxidant Enzyme Genetic Polymorphisms", Mol Diagn Ther, vol. 18, issue 1177-1062, pp. 55-62, 2014.
Xiong, X., S. Zhai, Z. Yao, T. Tazawa, H. -qing Zhao, Y. Li, M. R. Meselhy, N. Nakamura, T. Akao, and M. Hattori, "和漢医薬学雑誌= Journal of traditional medicines 20 (5), 201-207, 2003-10-20", Prog. Neurobiol, vol. 56, pp. 211-235, 1998. Abstract
n/a
Shoko), 河田尚子(K., 水谷周(M. Makoto), リーム・アハマド(R. Ahmad), 前野直樹(M. Naoki), 松山洋平(M. Yohei), and 松山朋子(M. Akiko), "イスラームにおける女性の位置づけーエジプトを中心に(Position of Women in Islam: focusing on Egypt)", イスラームと女性(Islam and Women), Tokyo, イスラーム信仰叢書(Islam Shinko Sosho), 2011.
NA, S., A. N, M. F. Ismail, K. YA, and S. M, "↓ Full text Ozone Therapy in Ethidium Bromide-Induced Demyelination in Rats: Possible Protective Effect. ", Cell Mol Neurobiol. , vol. 36, issue 6, pp. 943-954, 2016.
Youssef, M. A., L. Abdelsalam, R. A. Harfoush, I. M. Talaat, E. Elkattan, A. Mohey, R. M. A. Abdella, M. S. Farhan, H. A. Foad, A. M. Elsayed, et al., "• Prevalence of human papilloma virus (HPV) and its genotypes in cervical specimens of Egyptian women by linear array HPV genotyping test.", Infectious Agents and Cancer , vol. 11, 2016.
Ragai, M. F. R. F., Yasser.M.Abdlehamid, and Hamdy.A.Ahmed, "• Left ventricular hypertrophy and plasma Nitric oxide in hemodialysis patients. ", Life Science Journal, vol. 9 , issue 4, pp. 3280-3284, 2012.
Mohamed E. El Sayed, Yasir A. Bahadur, Ashraf Hassouna, and A. M. Nasr, "• High Dose Brachytherapy in Addition to External Beam Radiotherapy with or without Concurrent Chemotherapy in Cervix Uteri Cancer Patients: Clinical Results and Toxicity Profile", brachytherapy, vol. March–April, Volume 13, Pages S91–S92, issue Supplement 1,, pp. S91–S92, 2014.
EL-Maraghy, S. A., O. Adel, N. Zayed, A. Yosry, S. M. ElNahaas, and A. A. Gibriel, "• Circulatory miRNA-484, 524, 615 and 628 expression profiling in HCV mediated HCC among Egyptian patients; Implications for diagnosis and staging of hepatic cirrhosis and fibrosis ", Journal of Advanced Research , vol. 22, pp. 57-66, 2020.
Yang, J. D., E. A. Mohamed, A. A. O. Aziz, H. I. Shousha, and M. B. Hashem, "• Characteristics, management, and outcomes of patients with hepatocellular carcinoma in Africa: a multicountry observational study from the Africa Liver Cancer Consortium ", The Lancet Gastroenterology & Heptaology, vol. 2, issue 2, pp. p103–111, 2017.
Youssef, L., "“The Translation of the Term ‘Mubin’ in Surat Yasin in Yusuf Ali and Pickthall”", The Fifth International Symposium on Comparative Literature: Translation, The Department of English Language and Literature, Faculty of Arts, Cairo University, The Department of English Language and Literature, 1999.
Kamel, S., and L. Youssef, "“The Need for Standardising the Romanisation of Arabic”", Internationalizing the Arabic Language, Cairo, Cairo: Department of English, Faculty of Arts, 2015.
Youssef, L., and E. Paul and Janet Starkey, "“The Image of Nineteenth Century Cairo as a Medieval City in Kinglake’s Eothen”", Interpreting the Orient: Travellers in Egypt and the Near East, Lebanon, Ithaca Press, 2001.
Youssef, L., E. Z. Ibrahim, N. Kassabgy, and S. Aydelott, "“Teaching ‘Form’ in English Verse to Arabic Poetry Readers”", Diversity in Language, Cairo, The American University in Cairo, 2000.
Youssef, M., "“Superficial Bladder Cancer”, is a New Terminology Needed? the Problem of T1 G3 Tumours - an Egyptian Experience", European Journal of Surgical Oncology, vol. 38, issue 9, pp. 878, 2012.
Haggag, M., T. Yamashita, and H. S. Lee, "“Simulation of the North Indian Ocean Tropical Cyclones Using the Regional Environment Simulator: Application to Cyclone Nargis in 2008”: Indian Ocean Tropical Cyclones and Climate Change", Indian Ocean Tropical Cyclones and Climate Change: Springer Netherlands. https://doi.org/10.1007/978-90-481-3109-9, 2010.
Ahmad M. Yousef, Hesham M. Osman, Maged Georgy, and N. A. B. Yehia, "“Risk Assessment for Ad Hoc Construction Contracts” ", 13th Arab Structural Engineering Conference, , University of Blida, Algeria, 2015.
Youssef, L., "“Notes on the Friday Noon Prayer: Sheikh Mohammed Ghazāli"", “Toward, Around and Away From Tahrir: Tracking Expressions of Emerging Egyptian Identity, Cambridge, Cambridge Scholars Publishing, 2014.
Khalifa, O., and L. Youssef, "“Junkies on the Nile: Can the Translator of A ¼ Gram be Invisible?”", “Essays in Honour of Mohamed Enani” by Center of Languages and Translation, Cairo University, vol. Four, issue Four, pp. 299-311, 2012.
Youssef, L., "“Islamic Discourse: Shari’a or Islamic Law?”", Logos, Center for Languages and Translation, Cairo University, 2008.
Yousry, C., P. M. Zikry, H. M. Salem, E. B. Basalious, and O. N. El-Gazayerly, "“Integrated nanovesicular/selfnanoemulsifying system (INV/SNES) for enhanced dual ocular drug delivery: statistical optimization, in vitro and in vivo evaluation”", Drug Delivery and Translational Research. , vol. 3, pp. 801-814, 2020.
Nemr, R. A., M. Khalil, M. S. Sarhan, M. Abbas, H. ElSawey, H. H. Youssef, M. A. Hamza, A. T. Morsi, M. El-Tahan, M. Fayez, et al., "“In situ similis” Culturing of Plant Microbiota: A Novel Simulated Environmental Method Based on Plant Leaf Blades as Nutritional Pads", Frontiers in Microbiology, vol. 11, pp. 454, 2020. AbstractWebsite

High-throughput cultivation methods have recently been developed to accelerate the recovery of microorganisms reluctant to cultivation. They simulate in situ environmental conditions for the isolation of environmental microbiota through the exchange of growth substrates during cultivation. Here, we introduce leaf-based culture media adopting the concept of the plant being the master architect of the composition of its microbial community. Pre-physical treatments of sunflower plant leaves, namely punching, freezing, and/or autoclavation, allowed the diffusion of electrolytes and other nutrients to configure the leaf surface as a natural pad, i.e., creating an “in situ similis” environment suitable for the growth of rarely isolated microbiota. We used surface inoculation and membrane-filtration methods to assess the culturability of endophytic bacteria from the sunflower phyllosphere and rhizosphere. Both methods supported excellent colony-forming unit (CFU) development when compared to standard R2A medium, with a special affinity to support better growth of epiphytic and endophytic populations of the phyllosphere compared with the rhizosphere. A 16S rRNA gene analysis of >122 representative isolates indicated the cultivation of a diverse set of microorganisms by application of the new methods. It indicated the predominance of 13 genera of >30 potential species, belonging to Firmicutes, Proteobacteria, and Actinobacteria, and especially genera not commonly reported for sunflower, e.g., Rhizobium, Aureimonas, Sphingomonas, Paracoccus, Stenotrophomonas, Pantoea, Kosakonia, and Erwinia. The strategy successfully extended diversity and richness in the endophyllosphere compared to the endorhizosphere, while CFUs grown on the standard R2A medium mainly pertain to Firmicutes, especially Bacillus spp. MALDI-TOF MS analysis clustered the isolates according to their niche and potential functions, where the majority of isolates of the endorhizosphere were clustered away from those of the endophyllosphere. Isolates identified as Gammaproteobacteria and Alphaproteobacteria were distinguishably sub-clustered, which was in contrast to the heterogeneous isolates of Firmicutes (Bacillus spp.). In conclusion, leaf in situ similis cultivation is an effective strategy to support the future application of culturomics of plant microbiota. This is an effort to access novel isolates that are more adapted and competitive in their natural environments, especially those subjected to abiotic stresses like those prevailing in arid/semi-arid zones, and, consequently, to support the application of agro-biotechnologies, among other technologies, to improving agriculture in such zones.

Nemr, R. A., M. Khalil, M. S. Sarhan, M. Abbas, H. Elsawey, H. H. Youssef, M. A. Hamza, A. T. Morsi, M. EL-Tahan, M. Fayez, et al., "“In situ similis” Culturing of Plant Microbiota: A Novel Simulated Environmental Method Based on Plant Leaf Blades as Nutritional Pads", Frontiers in Microbiology, vol. 11, 2020. AbstractWebsite

High-throughput cultivation methods have recently been developed to accelerate the recovery of microorganisms reluctant to cultivation. They simulate in situ environmental conditions for the isolation of environmental microbiota through the exchange of growth substrates during cultivation. Here, we introduce leaf-based culture media adopting the concept of the plant being the master architect of the composition of its microbial community. Pre-physical treatments of sunflower plant leaves, namely punching, freezing, and/or autoclavation, allowed the diffusion of electrolytes and other nutrients to configure the leaf surface as a natural pad, i.e., creating an “in situ similis” environment suitable for the growth of rarely isolated microbiota. We used surface inoculation and membrane-filtration methods to assess the culturability of endophytic bacteria from the sunflower phyllosphere and rhizosphere. Both methods supported excellent colony-forming unit (CFU) development when compared to standard R2A medium, with a special affinity to support better growth of epiphytic and endophytic populations of the phyllosphere compared with the rhizosphere. A 16S rRNA gene analysis of >122 representative isolates indicated the cultivation of a diverse set of microorganisms by application of the new methods. It indicated the predominance of 13 genera of >30 potential species, belonging to Firmicutes, Proteobacteria, and Actinobacteria, and especially genera not commonly reported for sunflower, e.g., Rhizobium, Aureimonas, Sphingomonas, Paracoccus, Stenotrophomonas, Pantoea, Kosakonia, and Erwinia. The strategy successfully extended diversity and richness in the endophyllosphere compared to the endorhizosphere, while CFUs grown on the standard R2A medium mainly pertain to Firmicutes, especially Bacillus spp. MALDI-TOF MS analysis clustered the isolates according to their niche and potential functions, where the majority of isolates of the endorhizosphere were clustered away from those of the endophyllosphere. Isolates identified as Gammaproteobacteria and Alphaproteobacteria were distinguishably sub-clustered, which was in contrast to the heterogeneous isolates of Firmicutes (Bacillus spp.). In conclusion, leaf in situ similis cultivation is an effective strategy to support the future application of culturomics of plant microbiota. This is an effort to access novel isolates that are more adapted and competitive in their natural environments, especially those subjected to abiotic stresses like those prevailing in arid/semi-arid zones, and, consequently, to support the application of agro-biotechnologies, among other technologies, to improving agriculture in such zones. © Copyright © 2020 Nemr, Khalil, Sarhan, Abbas, Elsawey, Youssef, Hamza, Morsi, El-Tahan, Fayez, Patz, Witzel, Ruppel, El-Sahhar and Hegazi.

Sabet, S., M. A. George, H. A. I. D. A. N. M. EL-SHORBAGY, H. Bassiony, K. Y. Farroh, T. Youssef, and T. A. salah eldin, "“Gelatin nanoparticles enhance delivery of hepatitis C virus recombinant NS2 gene”", PLOS ONE, vol. 12, issue 7, pp. 1-15, 2017. journal.pone_.0181723-compressed.pdf
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