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Hasan, Ibrahim, and Waleed, "الاقتراض في العصر الحديث", مجلة هرمس, 2016. lqtrd_fy_lsr_lhdyth.pdf
Marie, M. - A. S., Fatma A. El-Deeb, H. S. Wafaa, and R. M. Atef, ‘Impact of seasonal water quality and trophic levels in the distribution of various freshwater snails in four Egyptian governorates.’, : Applied Ecology and Environmental Sciences, 3(4), 117-126 doi:10.12691/aees-3-4-4 , 2015.
Cebeci, T., E. E. Khalil, and J. Whitelaw, "“Calculation of Separated Boundary Layer Flows", AIAA Journal, , vol. 17, issue 2, pp. 1291-1298, 1978.
Wahed, M. A., S. A. Samy, and M. Montaser, "“Establishing an Accredited Medical Equipment Calibration Laboratory” ", 4th Cairo International Biomedical Engineering Conf. CIBEC 2008, Cairo, 2008.
Nemr, R. A., M. Khalil, M. S. Sarhan, M. Abbas, H. ElSawey, H. H. Youssef, M. A. Hamza, A. T. Morsi, M. El-Tahan, M. Fayez, et al., "“In situ similis” Culturing of Plant Microbiota: A Novel Simulated Environmental Method Based on Plant Leaf Blades as Nutritional Pads", Frontiers in Microbiology, vol. 11, pp. 454, 2020. AbstractWebsite

High-throughput cultivation methods have recently been developed to accelerate the recovery of microorganisms reluctant to cultivation. They simulate in situ environmental conditions for the isolation of environmental microbiota through the exchange of growth substrates during cultivation. Here, we introduce leaf-based culture media adopting the concept of the plant being the master architect of the composition of its microbial community. Pre-physical treatments of sunflower plant leaves, namely punching, freezing, and/or autoclavation, allowed the diffusion of electrolytes and other nutrients to configure the leaf surface as a natural pad, i.e., creating an “in situ similis” environment suitable for the growth of rarely isolated microbiota. We used surface inoculation and membrane-filtration methods to assess the culturability of endophytic bacteria from the sunflower phyllosphere and rhizosphere. Both methods supported excellent colony-forming unit (CFU) development when compared to standard R2A medium, with a special affinity to support better growth of epiphytic and endophytic populations of the phyllosphere compared with the rhizosphere. A 16S rRNA gene analysis of >122 representative isolates indicated the cultivation of a diverse set of microorganisms by application of the new methods. It indicated the predominance of 13 genera of >30 potential species, belonging to Firmicutes, Proteobacteria, and Actinobacteria, and especially genera not commonly reported for sunflower, e.g., Rhizobium, Aureimonas, Sphingomonas, Paracoccus, Stenotrophomonas, Pantoea, Kosakonia, and Erwinia. The strategy successfully extended diversity and richness in the endophyllosphere compared to the endorhizosphere, while CFUs grown on the standard R2A medium mainly pertain to Firmicutes, especially Bacillus spp. MALDI-TOF MS analysis clustered the isolates according to their niche and potential functions, where the majority of isolates of the endorhizosphere were clustered away from those of the endophyllosphere. Isolates identified as Gammaproteobacteria and Alphaproteobacteria were distinguishably sub-clustered, which was in contrast to the heterogeneous isolates of Firmicutes (Bacillus spp.). In conclusion, leaf in situ similis cultivation is an effective strategy to support the future application of culturomics of plant microbiota. This is an effort to access novel isolates that are more adapted and competitive in their natural environments, especially those subjected to abiotic stresses like those prevailing in arid/semi-arid zones, and, consequently, to support the application of agro-biotechnologies, among other technologies, to improving agriculture in such zones.

Nemr, R. A., M. Khalil, M. S. Sarhan, M. Abbas, H. Elsawey, H. H. Youssef, M. A. Hamza, A. T. Morsi, M. EL-Tahan, M. Fayez, et al., "“In situ similis” Culturing of Plant Microbiota: A Novel Simulated Environmental Method Based on Plant Leaf Blades as Nutritional Pads", Frontiers in Microbiology, vol. 11, 2020. AbstractWebsite

High-throughput cultivation methods have recently been developed to accelerate the recovery of microorganisms reluctant to cultivation. They simulate in situ environmental conditions for the isolation of environmental microbiota through the exchange of growth substrates during cultivation. Here, we introduce leaf-based culture media adopting the concept of the plant being the master architect of the composition of its microbial community. Pre-physical treatments of sunflower plant leaves, namely punching, freezing, and/or autoclavation, allowed the diffusion of electrolytes and other nutrients to configure the leaf surface as a natural pad, i.e., creating an “in situ similis” environment suitable for the growth of rarely isolated microbiota. We used surface inoculation and membrane-filtration methods to assess the culturability of endophytic bacteria from the sunflower phyllosphere and rhizosphere. Both methods supported excellent colony-forming unit (CFU) development when compared to standard R2A medium, with a special affinity to support better growth of epiphytic and endophytic populations of the phyllosphere compared with the rhizosphere. A 16S rRNA gene analysis of >122 representative isolates indicated the cultivation of a diverse set of microorganisms by application of the new methods. It indicated the predominance of 13 genera of >30 potential species, belonging to Firmicutes, Proteobacteria, and Actinobacteria, and especially genera not commonly reported for sunflower, e.g., Rhizobium, Aureimonas, Sphingomonas, Paracoccus, Stenotrophomonas, Pantoea, Kosakonia, and Erwinia. The strategy successfully extended diversity and richness in the endophyllosphere compared to the endorhizosphere, while CFUs grown on the standard R2A medium mainly pertain to Firmicutes, especially Bacillus spp. MALDI-TOF MS analysis clustered the isolates according to their niche and potential functions, where the majority of isolates of the endorhizosphere were clustered away from those of the endophyllosphere. Isolates identified as Gammaproteobacteria and Alphaproteobacteria were distinguishably sub-clustered, which was in contrast to the heterogeneous isolates of Firmicutes (Bacillus spp.). In conclusion, leaf in situ similis cultivation is an effective strategy to support the future application of culturomics of plant microbiota. This is an effort to access novel isolates that are more adapted and competitive in their natural environments, especially those subjected to abiotic stresses like those prevailing in arid/semi-arid zones, and, consequently, to support the application of agro-biotechnologies, among other technologies, to improving agriculture in such zones. © Copyright © 2020 Nemr, Khalil, Sarhan, Abbas, Elsawey, Youssef, Hamza, Morsi, El-Tahan, Fayez, Patz, Witzel, Ruppel, El-Sahhar and Hegazi.

Medhat, N., undefined, A. M. Wahed, and A. S. A. Mohamed, "“Medical Equipment Quality Assurance By Making Continuous Improvement To The System”.", 4th Cairo International Biomedical Engineering Conf. CIBEC 2008, Cairo, 2008.
Mohamed, A. S. A., A. M. Wahed, S. A. Samy, and N. Medhat, "“Medical Equipment Quality Assurance For Healthcare Facilities.”", 4th Cairo International Biomedical Engineering Conf. CIBEC 2008, Cairo, 2008.
Wahed, M. A., S. A. Samy, and M. Montaser, "“Root Cause Analysis for medical equipment calibration laboratory nonconformities“", 5th Cairo International Biomedical Engineering Conf. CIBEC 2010, Cairo, 2010.
Khalil, E. E., J. H. Whitelaw, and D. Gosman, "“The Calculation Of Two Dimensional Turbulent Recirculating Flows”", 1st Symposium On Turbulent Shear Flows, 1, 13.35, , September 1977.
W.A.Badawy, F. M. Al-Kharafi, and E. Y. Al-Hassan, "“The electrochemical Behaviour of Cadmium in Aqueous Solutions.”", Materials Science Forum , vol. 289-292, pp. 849-860, 1998.
W. Mohyeldin, M. G. Darwish, S. M. I. K., "“Video Streaming Over Ad-Hoc Networks Using Group of Picture Multiple Description Coding", International Journal of Intelligent Computing and Information Science, vol. 7, issue 2, pp. 55 – 69, 2007. Abstract

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Abdel-Aziz, H. A., M. M. Abdel-Aziz, A. G. Wassal, and A. A. Abou-Auf, "“Worst-Case Test Vectors Generation using Genetic Algorithm for the Detection of Total-Dose Leakage Current Failures", Proc. of 2010 IEEE 5th international Design and Test Workshop (IDT 2010), Abu Dhabi, UAE, Dec, 2010.
Elawady, D. M., M. Wagdy, A. Kaddah, and H. Omar, "• Evaluation of the effect of conventional and flexible acrylic denture bases on the supporting tissues in maxillary single denture cases", Egypt Dent J. 2010, vol. 59, pp. 2213 - 2218, 2010.
MME, N., E. - S. AY, S. MA, I. MA, A. - A. RA, and A. - R. WM, "• Prevalence of Factor VIII Inhibitors among Hemophilia A Patients", Nature and Science 2017;15(5), 69-73., vol. 15, issue 5, pp. 69-73, 2017. hemophilia_inh.pdf
MSM, A., A. MA, S. TM, A. AA, A. - A. WS, A. H, and K. OK, "• The effectiveness of high intensity laser therapy in the management of spinal disorders: A systematic review and meta-analysis", J Back Musculoskelet Rehabil., 2019.
AN, A. N. Z., D. Abdullah, A. Osman, E. - R. M. N. MN, N. Zayed, G. Esmat, E. W. W, and H. A. Hafez, "♣ Epstein-Barr virus and Interleukin-28B polymorphism in the prediction of response to interferon therapy in hepatitis C patients. ", .Arab J Gastroenterol. , vol. September–December 2015; 16, issue (3-4), pp. 84–89, 2015.
Wagih, A., " A Revolutionary Vision Reshaping History in Mahmoud Diyab's Gate to Conquest ", The 9th International Conference, The Department of the Greek and Latin Studies, Faculty of Arts, Cairo University, 2013.
Wagih, A., " Revival of Yoruba Heritage in Femi Osofisan's Esu and the Vagabond Minstrels ", 13th International Symposium on Comparative Literature, November 2016, 2017.
Wagih, A., " The Power of Marginalized Egyptian and Latina women: Disappointments and Aspirations ", The International Journal of the Humanities, vol. 9, issue 10, 2012.
Wagih, A., " The Power of the Silenced ", The Said and Unsaid: Papers on Language, Literature and Cultural Studies , Department of Foreign languages, University of Vlora "Ismail Qemali" Vlore, Albania, 2011.
Sherif, S. M., W. W. Wardakhan, and R. M. Mohareb, "-Enaminonitrilers in Heterocyclic Sytnthesis: A Novel one- pot Synthesis of Thiophenes and Their Fused Derivatives", J. of Chem. Research (1996), , pp. (M), 1970 (S), 356 , 1996.
Baker, G., E. Walied, and E. Hussein, " Endovascular Management of flush superficial femoral artery occlusion", Leipzig interventional course (LINC), Germany, 27 Janaury, 2013.
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