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Visvalingam, J., H. Wang, M. K. Youssef, J. Devos, C. O. Gill, and X. Yang, "Spatial and Temporal Distribution of Escherichia coli on Beef Trimmings Obtained from a Beef Packing Plant", Journal of Food Protection, vol. 79, issue 8, pp. 1325-1331, 2016. Abstract

The objective of this study was to determine the immediate source of Escherichia coli on beef trimmings produced at a large
packing plant by analyzing the E. coli on trimmings at various locations of a combo bin filled on the same day and of bins filled
on different days. Ten 2,000-lb (907-kg) combo bins (B1 through B10) of trimmings were obtained from a large plant on 6 days
over a period of 5 weeks. Thin slices of beef with a total area of approximately 100 cm2 were excised from five locations (four
corners and the center) at each of four levels of the bins: the top surface and 30, 60, and 90 cm below the top. The samples were
enriched for E. coli in modified tryptone soya broth supplemented with 20 mg/liter novobiocin. The positive enrichment cultures,
as determined by PCR, were plated on E. coli/coliform count plates for recovery of E. coli. Selected E. coli isolates were
genotyped using multiple-locus variable-number tandem repeat analysis (MLVA). Of the 200 enrichment cultures, 43 were
positive by PCR for E. coli, and 32 of these cultures yielded E. coli isolates. Two bins did not yield any positive enrichment
cultures, and three PCR-positive bins did not yield any E. coli isolates. MLVA of 165 E. coli isolates (30, 62, 56, 5, and 12 from
B6 through B10, respectively) revealed nine distinct genotypes. MLVA types 263 and 89 were most prevalent overall and on
individual days, accounting for 49.1 and 37.6% of the total isolates, respectively. These two genotypes were also found at
multiple locations within a bin. All nine genotypes belonged to the phylogenetic group A0 of E. coli, suggesting an animal origin.
The finding that the trimmings carried very few E. coli indicates an overall effective control over contamination of beef with E.
coli at this processing plant. The lack of strain diversity of the E. coli on trimmings suggests that most E. coli isolates may have
come from common sources, most likely equipment used in the fabrication process.

Vitrano, A., A. Meloni, W. A. Pollina, M. Karimi, A. El-Beshlawy, M. Hajipour, V. Di Marco, S. H. Ansari, A. Filosa, P. Ricchi, et al., "A complication risk score to evaluate clinical severity of thalassaemia syndromes.", British journal of haematology, vol. 192, issue 3, pp. 626-633, 2021. Abstract

The thalassaemia syndromes (TS) show different phenotype severity. Developing a reliable, practical and global tool to determine disease severity and tailor treatment would be of great value. Overall, 7910 patients were analysed with the aim of constructing a complication risk score (CoRS) to evaluate the probability of developing one or more complications. Nine independent variables were included in the investigation as predictors. Logistic regression models were used for Group A [transfusion-dependent thalassaemia (TDT)], Group B [transfused non-TDT (NTDT)] and Group C (non-transfused NTDT). Statistically significant predictors included age (years), haemoglobin levels, hepatic transaminases [alanine aminotransferase (ALT) and aspartate aminotransferase] and left-ventricular ejection fraction (LVEF) for Group A; age (years), age at first chelation (months), ALT and LVEF for Group B; and age (years), mean serum ferritin (SF) levels and LVEF for Group C. The area under the receiver operating characteristic curve was 84·5%, 82·1% and 80·0% for Groups A, Group B and Group C respectively, suggesting the models had good discrimination. Finally, the CoRS for each group was categorised into four risk classes (low, intermediate, high, and very high) using the centiles of its distribution. In conclusion, we have developed a CoRS for TS that can assist physicians in prospectively tailoring patients' treatment.

Viudes-de-Castro, M. P., R. Lavara, H. M. Safaa, F. Marco-Jiménez, G. M. K. Mehaisen, and J. S. Vicente, "Effect of freezing extender composition and male line on semen traits and reproductive performance in rabbits", animal, vol. 8, no. 05: Cambridge University Press, pp. 765–770, 2014. Abstract
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Viudes-de-Castro, M. P., R. Lavara, H. M. Safaa, F. Marco-Jiménez, G. M. K. Mehaisen, and J. S. Vicente, "Effect of freezing extender composition and male line on semen traits and reproductive performance in rabbits", Animal, issue In Press, pp. 1-6, 2014.
Viudes-de-Castro, M. P., R. Lavara, H. M. Safaa, F. Marco-Jiménez, G. M. K. Mehaisen, and J. S. Vicente, "Effect of freezing extender composition and male line on semen traits and reproductive performance in rabbits", animal, vol. 8, no. 05: Cambridge University Press, pp. 765–770, 2014. Abstract
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Viudes-de-Castro, M. P., R. Lavara, H. M. Safaa, F. Marco-Jiménez, G. M. K. Mehaisen, and J. S. Vicente, "Effect of freezing extender composition and male line on semen traits and reproductive performance in rabbits", animal, vol. 8, no. 05: Cambridge University Press, pp. 765–770, 2014. Abstract
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Vivante, A., D. - Y. Hwang, S. Kohl, J. Chen, S. Shril, J. Schulz, A. Van Der Ven, G. Daouk, N. A. Soliman, A. S. Kumar, et al., "Exome Sequencing Discerns Syndromes in Patients from Consanguineous Families with Congenital Anomalies of the Kidneys and Urinary Tract.", Journal of the American Society of Nephrology : JASN, vol. 28, issue 1, pp. 69-75, 2017 Jan. Abstract

Congenital anomalies of the kidneys and urinary tract (CAKUT) are the leading cause of CKD in children, featuring a broad variety of malformations. A monogenic cause can be detected in around 12% of patients. However, the morphologic clinical phenotype of CAKUT frequently does not indicate specific genes to be examined. To determine the likelihood of detecting causative recessive mutations by whole-exome sequencing (WES), we analyzed individuals with CAKUT from 33 different consanguineous families. Using homozygosity mapping and WES, we identified the causative mutations in nine of the 33 families studied (27%). We detected recessive mutations in nine known disease-causing genes: ZBTB24, WFS1, HPSE2, ATRX, ASPH, AGXT, AQP2, CTNS, and PKHD1 Notably, when mutated, these genes cause multiorgan syndromes that may include CAKUT as a feature (syndromic CAKUT) or cause renal diseases that may manifest as phenocopies of CAKUT. None of the above monogenic disease-causing genes were suspected on clinical grounds before this study. Follow-up clinical characterization of those patients allowed us to revise and detect relevant new clinical features in a more appropriate pathogenetic context. Thus, applying WES to the diagnostic approach in CAKUT provides opportunities for an accurate and early etiology-based diagnosis and improved clinical management.

Vivante, A., O. S. Chacham, S. Shril, R. Schreiber, S. M. Mane, B. Pode-Shakked, N. A. Soliman, I. Koneth, M. Schiffer, Y. Anikster, et al., "Dominant PAX2 mutations may cause steroid-resistant nephrotic syndrome and FSGS in children.", Pediatric nephrology (Berlin, Germany), vol. 34, issue 9, pp. 1607-1613, 2019. Abstract

BACKGROUND: Heterozygous PAX2 mutations cause renal coloboma syndrome (RCS) [OMIM no. 120330]. RCS is a renal syndromic disease encompassing retinal coloboma and sensorineural hearing loss. Recently, a causative role for PAX2 was reported in adult-onset nephrotic syndrome secondary to focal segmental glomerulosclerosis (FSGS). However, the prevalence of PAX2 mutations among large cohort of children with steroid-resistant nephrotic syndrome (SRNS) and FSGS has not been systematically studied.

METHODS: We employed whole-exome sequencing (WES) to identify the percentage of SRNS cases explained by monogenic mutations in known genes of SRNS/FSGS. As PAX2 mutations are not an established cause of childhood FSGS, we evaluated a cohort of 215 unrelated families with SRNS, in whom no underlying genetic etiology had been previously established.

RESULTS: Using WES, we identified 3 novel causative heterozygous PAX2 mutations in 3 out of the 215 unrelated index cases studied (1.3%). All three cases were detected in individuals from families with more than one affected and compatible with an autosomal dominant mode of inheritance (3/57 familial cases studied (5.2%)). The clinical diagnosis in three out of four pediatric index patients was done during routine medical evaluation.

CONCLUSIONS: Our findings demonstrate high frequency of PAX2 mutations in familial form of SRNS (5.2%) and further expand the phenotypic spectrum of PAX2 heterozygous mutations to include autosomal dominant childhood-onset FSGS. These results highlight the importance of including PAX2 in the list of genes known to cause FSGS in children.

Vivian H. Chu; Lawrence P. Park; Eugene Athan, F. D.; T. F.;;, C. L.; J. M.; D. M.; J. S.; C. M. W. Tribouilloy;, D. - M.; J. P.; N. F. لndez- H.; F. N.; H. M. Rizk;, and V. K.; E. G.; J. H.;M. H.; A. P. M. Wang;, "Association Between Surgical Indications, Operative Risk, and Clinical Outcome in Infective Endocarditis A Prospective Study From the International Collaboration on Endocarditis", Circulation, vol. 131, issue 131, pp. 131-140, 2015. circulation-2015-chu-131-40.pdf
Vlaar, A. P., P. M. T. Klooster, E. Taal, R. E. Gheith, A. K. El-Garf, J. J. Rasker, and M. A. V. de Laar, "A Cross-Cultural Study Of Pain Intensity In Egyptian And Dutch Women With Rheumatoid Arthritis", J Pain, vol. 9, issue 8, pp. 730-6, 2007.
Vlaar, A., P. ten Klooster, E. Taal, R. Gheith, A. El-Garf, J. Rasker, and M. van de Laar, "A Cross-Cultural Study of Pain Intensity in Egyptian and Dutch Women With Rheumatoid Arthritis", .J Pain, vol. 8, issue 9, pp. 730-736, 2007.
Vlasov, A. N., A. G. Shkvarunets, J. C. Rodgers, Y. Carmel, T. M. Antonsen Jr, T. M. Abuelfadl, D. Lingze, V. A. Cherepenin, G. S. Nusinovich, M. Botton, et al., "Overmoded GW-class surface-wave microwave oscillator", Plasma Science, IEEE Transactions on, vol. 28, no. 3: IEEE, pp. 550–560, 2000. Abstract
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Vogel, C. V., H. Pietraszkiewicz, O. M. Sabry, W. H. Gerwick, F. A. Valeriote, and C. D. Vanderwal, "Enantioselective divergent syntheses of several polyhalogenated Plocamium monoterpenes and evaluation of their selectivity for solid tumors.", Angewandte Chemie (International ed. in English), vol. 53, issue 45, pp. 12205-9, 2014 Nov 3. Abstract

The family of polyhalogenated monoterpenes from Plocamium counts over a hundred known members. Using glyceraldehyde acetonide as a chiral-pool precursor, an enantioselective and divergent strategy was developed that provides a blueprint for the synthesis of many of the small yet complex acyclic members of this family. The broad applicability of this approach is demonstrated with the short, eight-step synthesis of four natural products and three analogues. These syntheses are the first of any members of the acyclic polyhalogenated Plocamium monoterpenes and permitted the evaluation of their selectivity against a range of tumor cell lines.

VOGEL, C. V., H. PIETRASZKIEWICZ, S. A. B. R. Y. M. OMAR, W. H. GERWICK, F. A. VALERIOTE, and C. D. VANDERWAL, "Enantioselective, Divergent Syntheses of Several Polyhalogenated Plocamium Monoterpenes and Evaluation of their Selectivity for Solid Tumors", Angewandte Chem. Int. Ed. , vol. 53, issue 45, pp. 12205–12209, 2014. plocamium_monoterpenes.pdf
Vogl, T. J., N. - E. Nour-Eldin, S. Emad-Eldin, N. N. N. Naguib, J. Trojan, H. Ackermann, and O. Abdelaziz, "Portal Vein Thrombosis and Arterioportal Shunts: Effects on Tumor Response After Chemoembolization of Hepatocellular Carcinoma", World J. gastroenterol, vol. 17, issue 10, pp. 1267–1275, 2011. AbstractCU-PDF.pdf

IF: 2.240

Vogl, T. J., N. N. N. Naguib, T. Lehner, A. Nour-Eldin, K. Eichler, S. Zangos, and Tatjana, "Initial Experience with Repetitive Transarterial Chemoembolization (TACE) as a Third Line Treatment of Ovarian Cancer Metastasis to the Liver: Indications, Outcomes and Role in Patient's Management", Physical Review Letters, 2012. Abstract

Objective: To evaluate local tumor control and survival data after transarterial chemoembolization (TACE) with different drug combinations in the palliative third-line treatment of patients with ovarian cancer liver metastases.Methods: Sixty-five patients (mean age: 51.5 year) with unresectable hematogenous hepatic metastases of ovarian cancer who did not respond to systemic chemotherapy were repeatedly treated with TACE in 4-week intervals. The local chemotherapy protocol consisted of Mitomycin (group 1) (n=14; 21.5%),

Vogl, T. J., N. - E. A. Nour-Eldin, R. M. Hammerstingl, B. Panahi, and N. N. N. Naguib, "Microwave Ablation (MWA): Basics, Technique and Results in Primary and Metastatic Liver Neoplasms - Review Article.", RoFo : Fortschritte auf dem Gebiete der Rontgenstrahlen und der Nuklearmedizin, vol. 189, issue 11, pp. 1055-1066, 2017 Nov. Abstract

 The locoregional interventional oncological treatment approach is an accepted modality for liver neoplasms, especially for hepatocellular carcinoma (HCC) and oligonodular liver metastases.  The main aim of ablation therapies like microwave ablation (MWA) is to eradicate all malignant cells in a minimally invasive technique under imaging guidance while preserving the healthy tissue with a sufficient safety margin (at least 5 mm) surrounding the ablated lesion.  Ablation therapy can be performed via a percutaneous, laparoscopic or intraoperative approach under ultrasound, MRI or CT guidance for adequate localization and monitoring of the ablation process.  Ablation is the method of choice for oligonodular HCCs ≤ 3 cm. The technical success rate varies from 88 % to 98 % and progression-free survival (PFS) at 3 years from 27 % to 91.7 %. The same criteria apply to the therapy of liver metastases.   · Careful selection of patients proves to be essential for optimum results of MWA. · Interventionists should be familiar with all aspects of complication and rapid assessment of imaging methods in order to evaluate induced damage by thermal ablation. · MWA seems to have some advantages over radiofrequency ablation, like shorter ablation time, less pain, less heat sink effect; however, scientific proof is needed. · Vogl TJ, Nour-Eldin A, Hammerstingl RM et al. Microwave Ablation (MWA): Basics, Technique and Results in Primary and Metastatic Liver Neoplasms - Review Article. Fortschr Röntgenstr 2017; 189: 1055 - 1066.

Voils, S. A., E. J. Martin, B. M. Mohammed, A. Bayrlee, and D. F. Brophy, "Laboratory assessment of warfarin reversal with global coagulation tests versus international normalized ratio in patients with intracranial bleeding", Blood Coagulation & Fibrinolysis, vol. 26, issue 4: LWW, pp. 443-447, 2015. Abstract
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Voils, S. A., E. J. Martin, B. M. Mohammed, A. Bayrlee, and D. F. Brophy, Laboratory assessment of warfarin reversal with global coagulation tests versus international normalized ratio in patients with intracranial bleeding, , vol. 26, issue 4: LWW, pp. 443 - 447, 2015. Abstract
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Vongchan, P., M. Warda, H. Toyoda, T. Toida, R. M. Marks, and R. J. Linhardt, "Structural characterization of human liver heparan sulfate.", Biochimica et biophysica acta, vol. 1721, issue 1-3, pp. 1-8, 2005 Jan 18. Abstract

The isolation, purification and structural characterization of human liver heparan sulfate are described. 1H-NMR spectroscopy demonstrates the purity of this glycosaminoglycan (GAG) and two-dimensional 1H-NMR confirmed that it was heparan sulfate. Enzymatic depolymerization of the isolated heparan sulfate, followed by gradient polyacrylamide gel, confirmed its heparin lyase sensitivity. The concentration of resulting unsaturated disaccharides was determined using reverse phase ion-pairing (RPIP) HPLC with post column derivatization and fluorescence detection. The results of this analysis clearly demonstrate that the isolated GAG was heparan sulfate, not heparin. Human liver heparan sulfate was similar to heparin in that it has a reduced content of unsulfated disaccharide and an elevated average sulfation level. The antithrombin-mediated anti-factor Xa activity of human liver heparan sulfate, however, was much lower than porcine intestinal (pharmaceutical) heparin but was comparable to standard porcine intestinal heparan sulfate. Moreover, human liver heparan sulfate shows higher degree of sulfation than heparan sulfate isolated from porcine liver or from the human hepatoma Hep 2G cell line.

Vosoughi, A., T. Zhang, K. S. Shohdy, P. J. Vlachostergios, D. C. Wilkes, B. Bhinder, S. T. Tagawa, D. M. Nanus, A. M. Molina, H. Beltran, et al., "Common germline-somatic variant interactions in advanced urothelial cancer", Nature Communications, vol. 11, issue 1, pp. 6195 - 6195, 2020/12//. Abstract

The prevalence and biological consequences of deleterious germline variants in urothelial cancer (UC) are not fully characterized. We performed whole-exome sequencing (WES) of germline DNA and 157 primary and metastatic tumors from 80 UC patients. We developed a computational framework for identifying putative deleterious germline variants (pDGVs) from WES data. Here, we show that UC patients harbor a high prevalence of pDGVs that truncate tumor suppressor proteins. Deepening somatic loss of heterozygosity in serial tumor samples is observed, suggesting a critical role for these pDGVs in tumor progression. Significant intra-patient heterogeneity in germline-somatic variant interactions results in divergent biological pathway alterations between primary and metastatic tumors. Our results characterize the spectrum of germline variants in UC and highlight their roles in shaping the natural history of the disease. These findings could have broad clinical implications for cancer patients.

de Vries, N. L., V. van Unen, M. E. Ijsselsteijn, T. Abdelaal, R. van der Breggen, A. Farina Sarasqueta, A. Mahfouz, K. C. M. J. Peeters, T. Höllt, B. P. F. Lelieveldt, et al., "High-dimensional cytometric analysis of colorectal cancer reveals novel mediators of antitumour immunity.", Gut, 2019. Abstract

OBJECTIVE: A comprehensive understanding of anticancer immune responses is paramount for the optimal application and development of cancer immunotherapies. We unravelled local and systemic immune profiles in patients with colorectal cancer (CRC) by high-dimensional analysis to provide an unbiased characterisation of the immune contexture of CRC.

DESIGN: Thirty-six immune cell markers were simultaneously assessed at the single-cell level by mass cytometry in 35 CRC tissues, 26 tumour-associated lymph nodes, 17 colorectal healthy mucosa and 19 peripheral blood samples from 31 patients with CRC. Additionally, functional, transcriptional and spatial analyses of tumour-infiltrating lymphocytes were performed by flow cytometry, single-cell RNA-sequencing and multispectral immunofluorescence.

RESULTS: We discovered that a previously unappreciated innate lymphocyte population (LinCD7CD127CD56CD45RO) was enriched in CRC tissues and displayed cytotoxic activity. This subset demonstrated a tissue-resident (CD103CD69) phenotype and was most abundant in immunogenic mismatch repair (MMR)-deficient CRCs. Their presence in tumours was correlated with the infiltration of tumour-resident cytotoxic, helper and γδ T cells with highly similar activated (HLA-DRCD38PD-1) phenotypes. Remarkably, activated γδ T cells were almost exclusively found in MMR-deficient cancers. Non-activated counterparts of tumour-resident cytotoxic and γδ T cells were present in CRC and healthy mucosa tissues, but not in lymph nodes, with the exception of tumour-positive lymph nodes.

CONCLUSION: This work provides a blueprint for the understanding of the heterogeneous and intricate immune landscape of CRC, including the identification of previously unappreciated immune cell subsets. The concomitant presence of tumour-resident innate and adaptive immune cell populations suggests a multitargeted exploitation of their antitumour properties in a therapeutic setting.

Vural, A., H. Negm, and C. Vicini, "Rebotic Surgery of Skull Base", All Around the Nose: Springer, 2020.
Vwioko, E. D., M. A. El-Esawi, M. E. Imoni, A. A. Al-Ghamdi, H. M. Ali, M. M. El-Sheekh, E. A. Abdeldaym, and M. A. Al-Dosary, "Sodium Azide Priming Enhances Waterlogging Stress Tolerance in Okra (Abelmoschus esculentus L.)", Agronomy, vol. 9, no. 11: Multidisciplinary Digital Publishing Institute, pp. 679, 2019. Abstract
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