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Mohamed, H. T., V. Untereiner, I. Proult, S. A. A. Ibrahim, M. Götte, M. El-Shinawi, M. M. Mohamed, G. D. Sockalingum, and S. Brézillon, "Characterization of inflammatory breast cancer: a vibrational microspectroscopy and imaging approach at the cellular and tissue level.", The Analyst, vol. 143, issue 24, pp. 6103-6112, 2018 Dec 03. Abstract

Inflammatory breast cancer (IBC) has a poor prognosis because of the lack of specific biomarkers and its late diagnosis. An accurate and rapid diagnosis implemented early enough can significantly improve the disease outcome. Vibrational spectroscopy has proven to be useful for cell and tissue characterization based on the intrinsic molecular information. Here, we have applied infrared and Raman microspectroscopy and imaging to differentiate between non-IBC and IBC at both cell and tissue levels. Two human breast cancer cell lines (MDA-MB-231 and SUM-149), 20 breast cancer patients (10 non-IBC and 10 IBC), and 4 healthy volunteer biopsies were investigated. Fixed cells and tissues were analyzed by FTIR microspectroscopy and imaging, while live cells were studied by Raman microspectroscopy. Spectra were analyzed by hierarchical cluster analysis (HCA) and images by common k-means clustering algorithms. For both cell suspensions and single cells, FTIR spectroscopy showed sufficient high inter-group variability to delineate MDA-MB-231 and SUM-149 cell lines. Most significant differences were observed in the spectral regions of 1096-1108 and 1672-1692 cm-1. Analysis of live cells by Raman microspectroscopy gave also a good discrimination of these cell types. The most discriminant regions were 688-992, 1019-1114, 1217-1375 and 1516-1625 cm-1. Finally, k-means cluster analysis of FTIR images allowed delineating non-IBC from IBC tissues. This study demonstrates the potential of vibrational spectroscopy and imaging to discriminate between non-IBC and IBC at both cell and tissue levels.

Huben, N. B., A. A. Hussein, P. R. May, M. Whittum, C. Krasowski, Y. E. Ahmed, Z. Jing, H. Khan, H. L. Kim, T. Schwaab, et al., "Development of a Patient-Based Model for Estimating Operative Times for Robot-Assisted Radical Prostatectomy.", Journal of endourology, vol. 32, issue 8, pp. 730-736, 2018 Aug. Abstract

OBJECTIVES: To develop a methodology for predicting operative times for robot-assisted radical prostatectomy (RARP) using preoperative patient, disease, procedural, and surgeon variables to facilitate operating room (OR) scheduling.

METHODS: The model included preoperative metrics: body mass index (BMI), American Society of Anesthesiologists score, clinical stage, National Comprehensive Cancer Network risk, prostate weight, nerve-sparing status, extent and laterality of lymph node dissection, and operating surgeon (six surgeons were included in the study). A binary decision tree was fit using a conditional inference tree method to predict operative times. The variables most associated with operative time were determined using permutation tests. Data were split at the value of the variable that results in the largest difference in mean for surgical time across the split. This process was repeated recursively on the resultant data.

RESULTS: A total of 1709 RARPs were included. The variable most strongly associated with operative time was the surgeon (surgeons 2 and 4-102 minutes shorter than surgeons 1, 3, 5, and 6, p < 0.001). Among surgeons 2 and 4, BMI had the strongest association with surgical time (p < 0.001). Among patients operated by surgeons 1, 3, 5, and 6, RARP time was again most strongly associated with the surgeon performing RARP. Surgeons 1, 3, and 6 were on average 76 minutes faster than surgeon 5 (p < 0.001). The regression tree output in the form of box plots showed operative time median and ranges according to patient, disease, procedural, and surgeon metrics.

CONCLUSION: We developed a methodology that can predict operative times for RARP based on patient, disease and surgeon variables. This methodology can be utilized for quality control, facilitate OR scheduling, and maximize OR efficiency.

Shiratori, T., Y. Kyumoto-Nakamura, A. Kukita, N. Uehara, J. Zhang, K. Koda, M. Kamiya, T. Badawy, E. Tomoda, X. Xu, et al., "IL-1β Induces Pathologically Activated Osteoclasts Bearing Extremely High Levels of Resorbing Activity: A Possible Pathological Subpopulation of Osteoclasts, Accompanied by Suppressed Expression of Kindlin-3 and Talin-1.", Journal of immunology (Baltimore, Md. : 1950), vol. 200, issue 1, pp. 218-228, 2018 01 01. Abstract

As osteoclasts have the central roles in normal bone remodeling, it is ideal to regulate only the osteoclasts performing pathological bone destruction without affecting normal osteoclasts. Based on a hypothesis that pathological osteoclasts form under the pathological microenvironment of the bone tissues, we here set up optimum culture conditions to examine the entity of pathologically activated osteoclasts (PAOCs). Through searching various inflammatory cytokines and their combinations, we found the highest resorbing activity of osteoclasts when osteoclasts were formed in the presence of M-CSF, receptor activator of NF-κB ligand, and IL-1β. We have postulated that these osteoclasts are PAOCs. Analysis using confocal laser microscopy revealed that PAOCs showed extremely high proton secretion detected by the acid-sensitive fluorescence probe Rh-PM and bone resorption activity compared with normal osteoclasts. PAOCs showed unique morphology bearing high thickness and high motility with motile cellular processes in comparison with normal osteoclasts. We further examined the expression of Kindlin-3 and Talin-1, essential molecules for activating integrin β-chains. Although normal osteoclasts express high levels of Kindlin-3 and Talin-1, expression of these molecules was markedly suppressed in PAOCs, suggesting the abnormality in the adhesion property. When whole membrane surface of mature osteoclasts was biotinylated and analyzed, the IL-1β-induced cell surface protein was detected. PAOCs could form a subpopulation of osteoclasts possibly different from normal osteoclasts. PAOC-specific molecules could be an ideal target for regulating pathological bone destruction.

Kishawy, H. A., H. Hegab, U. Umer, and A. Mohany, Application of acoustic emissions in machining processes: analysis and critical review, , vol. 98, issue 5-8: Springer London, pp. 1391 - 1407, 2018. Abstract
Kluyts, H. - L., Y. le Manach, D. M. Munlemvo, F. Madzimbamuto, A. Basenero, Y. Coulibaly, S. Rakotoarison, V. Gobin, A. L. Samateh, M. S. Chaibou, et al., The ASOS Surgical Risk Calculator: development and validation of a tool for identifying African surgical patients at risk of severe postoperative complications, , vol. 121, issue 6, pp. 1357 - 1363, 2018. AbstractWebsite

BackgroundThe African Surgical Outcomes Study (ASOS) showed that surgical patients in Africa have a mortality twice the global average. Existing risk assessment tools are not valid for use in this population because the pattern of risk for poor outcomes differs from high-income countries. The objective of this study was to derive and validate a simple, preoperative risk stratification tool to identify African surgical patients at risk for in-hospital postoperative mortality and severe complications.
ASOS was a 7-day prospective cohort study of adult patients undergoing surgery in Africa. The ASOS Surgical Risk Calculator was constructed with a multivariable logistic regression model for the outcome of in-hospital mortality and severe postoperative complications. The following preoperative risk factors were entered into the model; age, sex, smoking status, ASA physical status, preoperative chronic comorbid conditions, indication for surgery, urgency, severity, and type of surgery.
The model was derived from 8799 patients from 168 African hospitals. The composite outcome of severe postoperative complications and death occurred in 423/8799 (4.8%) patients. The ASOS Surgical Risk Calculator includes the following risk factors: age, ASA physical status, indication for surgery, urgency, severity, and type of surgery. The model showed good discrimination with an area under the receiver operating characteristic curve of 0.805 and good calibration with c-statistic corrected for optimism of 0.784.
This simple preoperative risk calculator could be used to identify high-risk surgical patients in African hospitals and facilitate increased postoperative surveillance.
Clinical trial registration

Hegab, H., U. Umer, M. Soliman, and H. A. Kishawy, Effects of nano-cutting fluids on tool performance and chip morphology during machining Inconel 718, , vol. 96, issue 9-12: Springer London, pp. 3449 - 3458, 2018. Abstract
Hegab, H., H. A. Kishawy, M. H. Gadallah, U. Umer, and I. Deiab, On machining of Ti-6Al-4V using multi-walled carbon nanotubes-based nano-fluid under minimum quantity lubrication, , vol. 97, issue 5-8: Springer London, pp. 1593 - 1603, 2018. Abstract
Hegab, H., U. Umer, I. Deiab, and H. Kishawy, Performance evaluation of Ti–6Al–4V machining using nano-cutting fluids under minimum quantity lubrication, , vol. 95, issue 9-12: Springer London, pp. 4229 - 4241, 2018. Abstract
Bhangu, A., A. O. Ademuyiwa, M. L. Aguilera, P. Alexander, S. W. Al-Saqqa, G. Borda-Luque, A. Costas-Chavarri, T. M. Drake, F. Ntirenganya, E. J. Fitzgerald, et al., "Surgical site infection after gastrointestinal surgery in high-income, middle-income, and low-income countries: a prospective, international, multicentre cohort study", The Lancet Infectious DiseasesThe Lancet Infectious Diseases, vol. 18, issue 5: Elsevier, pp. 516 - 525, 2018. AbstractWebsite

BackgroundSurgical site infection (SSI) is one of the most common infections associated with health care, but its importance as a global health priority is not fully understood. We quantified the burden of SSI after gastrointestinal surgery in countries in all parts of the world.BackgroundSurgical site infection (SSI) is one of the most common infections associated with health care, but its importance as a global health priority is not fully understood. We quantified the burden of SSI after gastrointestinal surgery in countries in all parts of the world.

Salama, Z., A. Aboul-Enein, F. Abou-Elella, and undefined, "Active Constituents of Kiwi (Actinidia Deliciosa Planch) Peels and Their Biological Activities as Antioxidant, Antimicrobial and Anticancer", Research Journal of Chemistry and Environment, vol. 22, issue 9, 2018. habiba_2.pdf
Farag, N. F., S. H. ElAhmady, E. H. Abd Elrahman, A. Naumann, H. Schulz, S. M. Azzam, and undefined, "Characterization of essential oils from Myrtaceae species using ATR-IR vibrational spectroscopy coupled to chemometrics", Industrial Crops and Products, vol. 124, pp. 870-877, 2018.
Mohamed, S. H., A. L. El-Ansary, and undefined, "Determination of crystalline silica in respirable dust upon occupational exposure for Egyptian workers", Industrial Health, vol. 56, pp. 255-263, 2018.
M.B.Mostafa, A. I. Abdelgalil, and undefined, "Evaluation of Club Foot in Working Donkeys", World’s Veterinary Journal, vol. 8, issue 4, pp. 73-79, 2018. club_foot_donkeys.pdf
Mohammed, T. Y., H. A. El-Tawab, undefined, and M. O., "hekma", Der Pharmacia Lettre, vol. 10 (1), pp. 11-23, 2018.
wafaa mohamed, mai rifai, F. E. Z. Sadat, and undefined, "HPLC/DAD-MS CHARACTERISATION OF DIVERSE DYESTUFFS FROM A CASE STUDY OF HISTORIC FABRIC", Mediterranean Archaeology and Archaeometry, , vol. Vol. 18, No 1, issue 1, pp. 113-121, 2018. dye_characterisation.pdf
Zayed, E. A., A. A. Ainshoka, K. E. A. Shazly, and undefined, "Improvement of insulin resistance via increase of GLUT4 and PPARγ in metabolic syndrome‐induced rats treated with omega‐3 fatty acid or L‐carnitine", Biochem Mol Toxicol. 2018, pp. 1-6, 2018.
Ueno, N.T., Espinosa Fernandez, J.R., Cristofanilli, M., Overmoyer, B., Rea, D., Berdichevski, F., El-Shinawi, Bellon, J., Le-Petross, H.T., Lucci, A., et al., "International Consensus on the Clinical Management of Inflammatory Breast Cancer from the Morgan Welch Inflammatory Breast Cancer Research Program 10th Anniversary Conference.", J Cancer. , vol. 9, issue 8, pp. 1437-1447, 2018.
Mostafa, M. B., and undefined, "LINEAR AND ANGULAR BIOMETRIC MEASUREMENTS OF LIMBS OF CAMEL (Camelus dromedarius)", Journal of Camel Practice and Research, vol. 25 , issue 2, pp. 153-162, 2018. linear_and_angular__biometric.pdf
Abdelhady.M, undefined, and Abdelrhman.M, "Nanotechnology in surface of archaeological stones", Academia Journal of Agricultural Research, vol. 5, issue May 2018, pp. Academia Journal of Agricultural Research, 2018. abdelrhmam_muhammed.pdf
Ali, A. A., A. A. Gany, E. E. Zohairy, G. Hasabo, and undefined, "NEUROPSYCHOLOGICAL DISTURBANCE AMONG TRAMADOL USERS", international journal of biology,pharmacy and allied science, vol. 7, issue 2, pp. 192-203, 2018.
undefined, "A Novel Technique of Saddle Rhizotomy Using Thermal Radiofrequency for Intractable Perineal Pain in Pelvic Malignancy: A Pilot Study", Pain Physician 2018; 21:E651-E660 • ISSN 2150-1149, vol. 21, issue Pain Physician 2018; 21:E651-E660 • ISSN 2150-1149, pp. 651-660, 2018.
Hegab, H., H. A. Kishawy, M. H. Gadallah, U. Umer, and I. Deiab, "On machining of Ti-6Al-4V using multi-walled carbon nanotubes-based nano-fluid under minimum quantity lubrication", The International Journal of Advanced Manufacturing Technology, 2018. as6218729662873671525277477297_content_1.pdf