The mechanical vibration is applied to a spacecraft via the interface to a launch vehicle at lift-off. The spacecraft is also exposed to acoustic pressure with wide frequency range. Lightweight and large area structures, such as solar paddles, antennas, and components with relatively high natural frequencies, are sensitive to acoustic load. Although the acoustic tests are usually conducted for components and system of the spacecraft, acoustic tests and analysis of the spacecraft mounted in a fairing have not been sufficiently done. It is expected that numerical analysis can be applied to the prediction of acoustic environment inside of fairings. Japan Aerospace Exploration Agency (JAXA) has been developing a vibroacoustic prediction tool by the wave based method (WBM), which is one of the deterministic approaches and is proposed for numerical prediction up to the mid-frequency range. The existing deterministic approaches can not accurately solve vibroacoustic problems in this range. In this paper, numerical prediction results of sound transmission by the 3D WBM and FEM are compared with those by an experiment, and verification of the 3D WBM is examined.

Clear cell sarcoma of the kidney (CCSK) accounts for 2-5% of all pediatric renal malignancies, which is known for its probability to metastasize to bone and other sites. We are reporting two cases with bilateral CCSK that were diagnosed at our institution. One patient initially presented with bilateral renal masses, as well as pulmonary, hepatic and bone metastasis; while other present only with bilateral masses with no evident distant metastasis. Both patients received aggressive neo-adjuvant chemotherapy to decrease tumor size. One patient completed his designated treatment and initially showed complete remission (CR); eventually suffering from relapse. The other patient's tumor progressed during the course of chemotherapy. Both cases manifested brain dissemination at the time of relapse or progression. This emphasizes the importance of staging stratification in CCSK. This also illustrates CCSK's ability to metastasize to bone and other sites including the brain (a primary relapse site in our cases).

Green construction management is an approach that aims to promote sustainable solutions in building design and construction. However, the complexity and variability in design scenarios often pose challenges to implementing these practices effectively. This study proposes a collaborative framework for green construction management that leverages automated and semi-automated simulations to facilitate performance-based decision making. The framework utilizes the green pyramid rating system (GPRS) as a third-party certification assessment provider. It employs various BIM analysis software tools, including Dynamo codes in Autodesk Revit and add-in plug-ins using Revit API, to extract information and parameters from the BIM model. The data are used to perform quantitative calculations and comprehensive analysis that aid decision makers in investigating, analyzing, improving, and assessing sustainability aspects throughout the project. The proposed framework enables a structured approach to green construction management that incorporates the building variable consecutive level of details/development (LOD) and life cycle phases. A case study of an office building in the new administrative capital in Egypt demonstrates the effectiveness of the framework in promoting sustainable solutions. The collaborative framework for interoperability design decisions proposed in this study provides a valuable tool for implementing green construction management practices. The use of automated and semi-automated simulations, following the GPRS, helps to ensure that sustainable solutions are advocated, interrogated, and refined throughout the project. The framework’s structured approach enables decision makers to investigate, analyze, improve, and assess sustainability aspects effectively.

The retarded development of parthenote embryo could be due to aberrant epigenetic imprinting, which may disrupt many aspects and lead to conceptus demise. The present work was conducted to: 1) compare the development of in vitro produced (IVP) and parthenogenetically developed (P) buffalo embryos from the 2-cell to blastocyst stage; 2) investigate the global gene expression profile and search for new candidate transcripts differing between IVP and P buffalo blastocyst using cDNA microarray analysis (validated by Real Time PCR); 3) follow the particular expression patterns of PLAC8 and OCT4 genes at five different stages of preimplantation development by Real Time PCR; and 4) study the expression of CDX2 at the blastcocyst stage. Cleavage rate was higher (P < 0.05) in P than IVP buffalo embryos, while, progression to blastocyst and number of cells per blastocyst were lower (P < 0.05) in P than IVP blastocysts. Microarray analysis indicate that 56 differentially expressed genes between the two groups, of which 51 genes (91.07%) were up-regulated, and five genes were downregulated in IVP blastocyst, using 1.4 fold-changes as a cutoff. Differentially expressed genes are related to translation, nucleic acid synthesis, cell adhesion and placentation. Validation of candidate genes revealed that the transcript abundance of PTGS2, RPS27A, TM2D3, PPA1, AlOX15, RPLO and PLAC8 were downregulated (7/8) in parthenote blastocyst compared to the IVP blastocyst. PLAC8 gene expression was higher (P < 0.05) at 2-cell, morula and blastocyst stages in IVP embryos compared with parthenote embryos. The OCT4 gene expression was higher (P < 0.05) in 2-cell, 4-cell, 8-cell and blastocysts produced in vitro. In conclusion, the retarded development of parthenogenetic buffalo embryos could be due to downregulation of genes related to translation, nucleic acid synthesis, cell adhesion, and placental development. The low expression of PLAC8 and OCT4 during the different stages of development may be responsible, in part, to the failure of development of parthenote buffalo embryos.