Hatcheries have the power to spread antimicrobial resistant (AMR) pathogens through the poultry value chain because of their central position in the poultry production chain. Currently, no information is available about the presence of AMR Escherichia coli strains and the antibiotic resistance genes (ARGs) they harbor within hatchezries. Therefore, this study aimed to investigate the possible involvement of hatcheries in harboring hemolytic AMR E. coli. Serotyping of the 65 isolated hemolytic E. coli revealed 15 serotypes with the ability to produce moderate biofilms, and shared susceptibility to cephradine and fosfomycin and resistance to spectinomycin. The most common β-lactam resistance gene was bla, followed by bla, bla-like bla-like bla and bla. Hierarchical clustering of E. coli isolates based on their phenotypic and genotypic profiles revealed separation of the majority of isolates from hatchlings and the hatchery environments, suggesting that hatchling and environmental isolates may have different origins. The high frequency of β-lactam resistance genes in AMR E. coli from chick hatchlings indicates that hatcheries may be a reservoir of AMR E. coli and can be a major contributor to the increased environmental burden of ARGs posing an eminent threat to poultry and human health.

Analysis of the SARS-CoV-2 sequence revealed a multibasic furin cleavage site at the S1/S2 boundary of the spike protein distinguishing this virus from SARS-CoV. Furin, the best-characterized member of the mammalian proprotein convertases, is an ubiquitously expressed single pass type 1 transmembrane protein. Cleavage of SARS-CoV-2 spike protein by furin promotes viral entry into lung cells. While furin knockout is embryonically lethal, its knockout in differentiated somatic cells is not, thus furin provides an exciting therapeutic target for viral pathogens including SARS-CoV-2 and bacterial infections. Several peptide-based and small-molecule inhibitors of furin have been recently reported, and select cocrystal structures have been solved, paving the way for further optimization and selection of clinical candidates. This perspective highlights furin structure, substrates, recent inhibitors, and crystal structures with emphasis on furin's role in SARS-CoV-2 infection, where the current data strongly suggest its inhibition as a promising therapeutic intervention for SARS-CoV-2.

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Recent years have seen growing appreciation that rapidly intensifying “flash droughts” are significant climate hazards with major economic and ecological impacts. This has motivated efforts to inventory, monitor, and forecast flash drought events. Here we consider the question of whether the term “flash drought” comprises multiple distinct classes of event, which would imply that understanding and forecasting flash droughts might require more than one framework. To do this, we first extend and evaluate a soil moisture volatility-based flash drought definition that we introduced in previous work and use it to inventory the onset dates and severity of flash droughts across the Contiguous United States (CONUS) for the period 1979-2018. Using this inventory, we examine meteorological and land surface conditions associated with flash drought onset and recovery. These same meteorological and land surface conditions are then used to classify the flash droughts based on precursor conditions that may represent predictable drivers of the event. We find that distinct classes of flash drought can be diagnosed in the event inventory. Specifically, we describe three classes of flash drought: “dry and demanding” events for which antecedent evaporative demand is high and soil moisture is low, “evaporative” events with more modest antecedent evaporative demand and soil moisture anomalies, but positive antecedent evaporative anomalies, and “stealth” flash droughts, which are different from the other two classes in that precursor meteorological anomalies are modest relative to the other classes. The three classes exhibit somewhat different geographic and seasonal distributions. We conclude that soil moisture “flash droughts” are indeed a composite of distinct types of rapidly intensifying droughts, and that flash drought analyses and forecasts would benefit from approaches that recognize the existence of multiple phenomenological pathways.

Nuclear reactions between two heavy interacting ions with nucleon transfer have been reconsidered. The direct nuclear reaction mechanism is considered. Different reaction processes are considered for single neutron or proton stripping and pickup reactions. The interacting nuclear potential of the transferred nucleon is taken to have the form of the Skyrme-type potential. With this representation for the nuclear potential of the transferred nucleon, an expression for the differential cross section is obtained by using the distorted-wave Born approximation. This expression is applied and considered for the heavy ion reactions with incident heavy ion projectiles B10, C13, O16, O18, and S32 bombarding the heavy targets Al27, Si28, Si29, Si30, and S32. The energies of the incident heavy ions have values in the range between 36.0 and 100.0 MeV. Numerical calculations of the differential cross sections are carried out. The theoretically calculated angular distributions are in good agreements with the experimental measurements. Reasonable spectroscopic factors are extracted from the present calculations. NUCLEAR REACTIONS Nucleon stripping and nucleon pickup induced by 36.0-100.0 MeV B10, C13, O16, O18, and S32 on Al27, Si28, Si29, Si30, and S32; calculated σ(θ). Finite range DWBA calculations, extracted spectroscopic factors. © 1983 The American Physical Society.