Publications

Export 853 results:
Sort by: [ Author  (Desc)] Title Type Year
A B C D E F G H I J K [L] M N O P Q R S T U V W X Y Z   [Show ALL]
L
Loutfy, S. A., M. M. Moneer, S. E. Salem, E. A. El-Moniem Abada, E. A. El-Moniem Ahmed, L. H. Ibrahim, and E. - C. B. Mohamed, "Polyomavirus infections and its clinical relevance in cancer patients: A Prospective Study", Journal of Infection and Public Health, vol. 10, issue 1, pp. 22 - 30, 2017. Abstract

BK and JC polyomaviruses (PyV) have been demonstrated to be associated with the pathogenesis of various human cancers. We aimed to investigate the impact of BK and JC polyomavirus infections on several clinical parameters in different human cancers. A total of 150 cancer patients were included in the study (51 patients with solid tumors, 48 patients with lymphomas and 51 patients with leukemias). Amplification of PyV DNA was performed using a semi-nested version of Polymerase chain reaction targeting the T genomic region of PyV. The polyomavirus load was determined using real-time PCR assay. The clinical data were collected. Polyomavirus DNA could be detected in 84 (56%) of 150 of all cancerous patients. The solid tumors had the lowest proportion of JCV (6 (11.8%) of 51), whereas had the highest proportion of JCV (200copies/μl). JCV was more frequent among NHL patients (30%) and absent in HL patients (0%). During follow-up, PyV positivity decreased significantly (p=0.004) in lymphoma patients (n=28). Although PyV positivity decreased significantly from 39% to 7% in 28 of 48 lymphoma patients after treatment, it significantly persisted in leukemic patients after treatment (from 22% to 38%). JC was more frequent among leukemic patients with leukopenia. The presence of JC polyomavirus was more frequent among leukemic patients without any significant impact on their overall survival.

loutfy, S. A., M. M. Moneer, S. E. S. Mohamed, E. A. E. - M. Abada, E. A. E. - M. Ahmed, L. Hussein, and E. L. - C. B. Mohamed, "Polyomavirus infections and its clinical relevance in cancer patients: A Prospective Study.", Journal of Infection and Public Health, vol. 10, issue 1, pp. 22-30, 2017.
Loutfy, S. A., M. B. Mohamed, N. T. Abdel-Ghani, N. Al-Ansary, W. A. Abdulla, O. M. El-Borady, Y. Hussein, and H. M. Eldin, "Metallic Nanomaterials as Drug Carriers to Decrease Side Effects of Chemotherapy (In Vitro: Cytotoxicity Study)", Journal of Nanopharmaceutics and Drug Delivery, vol. 1, pp. 1-12, 2013.
Loutfy, S. A., Z. F. Abdallah, M. Shaalan, M. Moneer, A. Karam, M. M. Moneer, I. M. Sayed, A. A. Abd El-Hafeez, P. Ghosh, and A. - R. N. Zekri, "Prevalence of MMTV-Like Sequences and Its Association with BRCA1/2 Genes Mutations Among Egyptian Breast Cancer Patients.", Cancer management and research, vol. 13, pp. 2835-2848, 2021. Abstract

Background: Mouse mammary tumor virus (MMTV) is thought to have a role in human breast cancer (BC) pathogenesis. BRCA1 and 2 genes mutations are well-established risk factors for BC. The purpose of this study was to evaluate the presence of MMTV in familial and non-familial Egyptian breast cancer patients. We also aimed to establish a correlation between BRCAs genes mutations and MMTV infection in those patients.

Patients and Methods: The study was included 80 BC patients and 10 healthy women were included as a control group. We used PCR to amplify a 250-bp MMTV-like env sequence. We also used PCR followed by direct sequencing to identify the genetic variation of exons 2, 13, 19 of BRCA1 gene and exon 9 and region f of exon 11 of BRCA2 gene. High resolution melting (HRM) analysis was used to screen the selected exons of BRCA1/2 genes in order to detect different variants.

Results: MMTV DNA-like env sequences were detected in 70%, 76% of familial and non-familial BC patients, respectively, and it was not detected in any of the control subjects. The presence of viral sequences was associated with larger tumor size in the sporadic patients. Seventy BC patients showed variations in BRCA1/2 genes according to HRM analysis and sequencing analysis showed two different sequences of polymorphism among 22 familial and non-familial BC patients.

Conclusion: MMTV DNA was present among BC patients and it was associated with increased tumor growth. This indicates a potential role for MMTV in BC patients with and without deleterious mutation in BRCA1/2 genes.

Loutfy, S. A., M. H. Elberry, K. Y. Farroh, H. T. Mohamed, A. A. Mohamed, E. C. B. Mohamed, A. H. I. Faraag, and S. A. Mousa, "Antiviral Activity of Chitosan Nanoparticles Encapsulating Curcumin Against Hepatitis C Virus Genotype 4a in Human Hepatoma Cell Lines.", International journal of nanomedicine, vol. 15, pp. 2699-2715, 2020. Abstract

Purpose: Current direct-acting antiviral agents for treatment of hepatitis C virus genotype 4a (HCV-4a) have been reported to cause adverse effects, and therefore less toxic antivirals are needed. This study investigated the role of curcumin chitosan (CuCs) nanocomposite as a potential anti-HCV-4a agent in human hepatoma cells Huh7.

Methods: Docking of curcumin and CuCs nanocomposite and binding energy calculations were carried out. Chitosan nanoparticles (CsNPs) and CuCs nanocomposite were prepared with an ionic gelation method and characterized with TEM, zeta size and potential, and HPLC to calculate encapsulation efficiency. Cytotoxicity studies were performed on Huh7 cells using MTT assay and confirmed with cellular and molecular assays. Anti-HCV-4a activity was determined using real-time PCR and Western blot.

Results: The strength of binding interactions between protein ligand complexes gave scores with NS3 protease, NS5A polymerase, and NS5B polymerase of -124.91, -159.02, and -129.16, for curcumin respectively, and -68.51, -54.52, and -157.63 for CuCs nanocomposite, respectively. CuCs nanocomposite was prepared at sizes 29-39.5 nm and charges of 33 mV. HPLC detected 4% of curcumin encapsulated into CsNPs. IC50 was 8 µg/mL for curcumin and 25 µg/mL for the nanocomposite on Huh7 but was 25.8 µg/mL and 34 µg/mL on WISH cells. CsNPs had no cytotoxic effect on tested cell lines. Apoptotic genes' expression revealed the caspase-dependent pathway mechanism. CsNPs and CuCs nanocomposite demonstrated 100% inhibition of viral entry and replication, which was confirmed with HCV core protein expression.

Conclusion: CuCs nanocomposite inhibited HCV-4a entry and replication compared to curcumin alone, suggesting its potential role as an effective therapeutic agent.

Loutfy, S., R. Shalaby, A. Hamed, M. Mohamed, A. Barakat, Z. Abdullah, H. Yousef, A. Tamim, H. Eldin, and N. Hegazy, Evaluation of cytotoxic effect of metallic nanoparticles in an in Vitro liver cancer model, , vol. 2015, pp. 470 - 487, 2015/06/30. Abstract

We aim to evaluate cytotoxic effect of silver and gold metallic nanoparticles (AgNPs & AuNPs) on human cucasian hepatocellular carcinoma cell line model (HepG2) and their possible anti-proliferative activity. This new class of engineered nanoparticles with desired physicochemical properties can be applied as new therapeutic approaches against human liver cancer disease. HepG2 was used as a model of human liver cancer cells. Metallic nanoparticles were characterized using UV-visible spectra and transmission electron microscopy (TEM). Cytotoxic effects of metallic nanoparticles on HepG2 cells were followed by colorimetric neutral red and SRB cell viability assays. Further investigation of cytotoxic effect of our nanomaterials were further investigated on a cellular and molecular level using cell cycle analysis , DNA and some apoptotic genes expression on a level of mRNA for P53, Bak, Bax, BCl 2 and β actin was served as housekeeping gene. Treatment of HepG2 with different concentrations of 22 nm diameter of AgNPs did not show alteration of cell morphology after 24 h of cell exposure. Also, when cells were treated with high concentration of AgNPs (viability was 78% after cell treatment with 10 µM and decreased to 46% after treatment of cells with 1000 µM). Cellular evaluation of AgNPs revealed progressive accumulation in the S phase of the cell cycle correlating with decreased number of cells in the G2/M phase followed by cellular DNA fragmentation. Extensive evaluation of cytotoxic effect of AgNPs showed mRNA apoptotic genes expression (P53, Bak, Bax, BCl 2) without expression of mRNA of caspase 3 gene which was expressed in untreated cells, suggesting involvement of intrinsic apoptotic caspase independent pathway. Treatment of HepG-2 with different concentrations of 34 nm diameter of AuNPs did not show alteration of cell morphology after 24 h of cell exposure. Such metallic nanoparticles did not reveal toxic effect at concentration up to 50 µM after 48 h of cell exposure. Cellular evaluation of AuNPs revealed progressive accumulation at G0/G1and at G2/M phases of cell cycle. Also the same results were obtained by treating cells with AgNPs, where the expression of mRNA of P53, Bak, Bax, BCl 2 without expression of mRNA of caspase 3 gene was observed in treated cells, suggesting intrinsic apoptotic caspase independent mechanism but may be induced by different molecules than that exerted by AgNPs. Our engineered silver nanoparticles at size of 22nm revealed genotoxic effect on human liver carcinoma cell line HepG-2 through intrinsic apoptotic caspase independent mechanisms. Further quantitative analysis and investigation of impact of time on genotoxic effect are required before reaching a final conclusion and starting in vivo assays.

Loutfy, S. A., M. M. Moneer, S. E. Salem, E. A. El-Moniem Abada, E. A. El-Moniem Ahmed, L. H. Ibrahim, and E. - C. B. Mohamed, "Polyomavirus infections and its clinical relevance in cancer patients: A Prospective Study.", Journal of infection and public health, vol. 10, issue 1, pp. 22-30, 2017 Jan - Feb. Abstract

BK and JC polyomaviruses (PyV) have been demonstrated to be associated with the pathogenesis of various human cancers. We aimed to investigate the impact of BK and JC polyomavirus infections on several clinical parameters in different human cancers. A total of 150 cancer patients were included in the study (51 patients with solid tumors, 48 patients with lymphomas and 51 patients with leukemias). Amplification of PyV DNA was performed using a semi-nested version of Polymerase chain reaction targeting the T genomic region of PyV. The polyomavirus load was determined using real-time PCR assay. The clinical data were collected. Polyomavirus DNA could be detected in 84 (56%) of 150 of all cancerous patients. The solid tumors had the lowest proportion of JCV (6 (11.8%) of 51), whereas had the highest proportion of JCV (200copies/μl). JCV was more frequent among NHL patients (30%) and absent in HL patients (0%). During follow-up, PyV positivity decreased significantly (p=0.004) in lymphoma patients (n=28). Although PyV positivity decreased significantly from 39% to 7% in 28 of 48 lymphoma patients after treatment, it significantly persisted in leukemic patients after treatment (from 22% to 38%). JC was more frequent among leukemic patients with leukopenia. The presence of JC polyomavirus was more frequent among leukemic patients without any significant impact on their overall survival.

Loutfi, M., M. Saleh, and H. Farouk, "Training Managers in the Developing World", Future of Arab Education Journal, vol. 15, no. 56: Ain Shams University Egypt, pp. 5-22, 2009. Abstract
n/a
Loutfi, M., A. Moscardini, M. Saleh, and H. Farouk, "Training Egyptian Middle Management On System Dynamics", Proceedings of the 24th International Conference of the System Dynamics Society, 2006. Abstract
n/a
Louis, D., and A. Rashad, "Formulation of Nano-sized dispersion from Calendula Officinalis: A Natural gateway to preparation of a Hair Dye", Research journal of pharmacy and technology, vol. 16, issue 4, pp. 1815-1822, 2023.
Louis, M. M., A. Bdawi, D. I. Nessem, and N. S. AbdelMalak, "Drotaverine hydrochloride gastroretentive floating mini-tablets: Formulation, in-vitro and in-vivo evaluation", journal of drug delivery science and technology, vol. 57 , pp. 101733, 2020. mina_paper_2020.pdf
Louis, D., "Formulation and Evaluation of Nanocrystals of a Lipid Lowering Agent", Iranian Journal of Pharmaceutical Sciences 2016, vol. 15, issue 1, pp. 71-82, 2016.
Louis, M. M., A. Bdawi, D. I. Nessem, and N. S. AbdelMalak, "Drotaverine hydrochloride gastroretentive floating mini-tablets: Formulation, in-vitro and in-vivo evaluation", journal of drug delivery science and technology, vol. 57 , pp. 101733, 2020. mina_paper_2020.pdf
Lotti, F., F. Frizza, G. Balercia, A. Barbonetti, H. M. Behre, A. E. Calogero, J. - F. Cremers, F. Francavilla, A. M. Isidori, S. Kliesch, et al., "The European Academy of Andrology (EAA) ultrasound study on healthy, fertile men: clinical, seminal and biochemical characteristics", Andrology, vol. 8, issue 5, pp. 1005-1020, 2020. 3_-the_european_academy_of_andrology_eaa_ultrasound_study.pdf
Lotfy, K. H., R. M. El-Sherbiny, and A. M. Salem, "Three Dimensional Effects of Vertical Stiffeners on Infinitely-Long Sheet-Piles in Cohesionless Soil", The International Foundations Congress and Equipment Expo, San Antonio, TX, USA, ASCE, GSP 252, pp. 1449-1463, 2015.
Lotfy, H. M., S. S. Saleh, and C. M. El-Maraghy, "Advanced approaches for the treatment and amplification of weakspectral signals produced by critical concentrations in whitemulticomponent systems", Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy , vol. 224, pp. 117339, 2020.
Lotfy, H. M., and S. S. Saleh, "ADVANCED AMPLITUDE CENTERING AS AN INVIGORATING MANIPULATION FOR UNIFIED WAVELENGTH SPECTRAL RESOLUTION OF TERNARY MIXTURES", International Journal of Pharmacy and Pharmaceutical Sciences, vol. Vol 9,, , issue Issue 5, pp. 43-50, 2017. advanced_amplitude_modulation_16960-87563-2-pb_1_1.pdf
Lotfy, A., A. Hasanin, M. Rashad, M. Mostafa, D. Saad, M. Mahmoud, W. Hamimy, and A. Z. Fouad, "Peripheral perfusion index as a predictor of failed weaning from mechanical ventilation", Journal of Clinical Monitoring and Computing, vol. 35, issue 2: Springer Science and Business Media B.V., pp. 405 - 412, 2021. AbstractWebsite
n/a
Lotfy, D. M., M. M. Safar, S. H. M. Hassan, and S. A. Kenawy, "Modulation of PTZ-induced convulsions in rats using topiramate alone or combined with low dose gamma irradiation: involving AKT/m-TOR pathway.", Toxicology mechanisms and methods, vol. 32, issue 1, pp. 18-26, 2022. Abstract

The current study evaluates the anticonvulsant effect low dose whole body gamma irradiation (LDR) alone or combined with topiramate against pentylenetetrazole (PTZ)-induced convulsions. Male Wister rats received either saline or PTZ (75 mg/kg i.p.). The other three groups were pretreated with single low dose radiation (0.5 Gy), topiramate (50 mg/kg, p.o., seven days) and TPM with LDR respectively before PTZ injection. Racine' score, latency, and duration of the convulsions were assessed. Glutamate and GABA were measured. AKT/m-TOR signaling pathway including AKT (protein kinase B), mammalian target of rapamycin (m-TOR), protein S6, and caspase 3 were also assessed. Measurements of markers of oxidative stress including malondialdehyde (MDA), glutathione (GSH), and nitric oxide (NO) were carried out. Histological examinations of hippocampi were done. PTZ produced behavioral changes (high Racine score, short latency, and long duration). It elevated MDA and NO contents, while reduced GSH content. TPM treatment alone or combined with LDR ameliorated the PTZ-induced convulsions and caused significant improvement in behavioral changes, brain mediators, m-TOR pathway, oxidative stress, and histological pictures in hippocampal regions. Histopathological examinations of the normal group showed normal structure with intact cells, while PTZ-treated rats exhibited necrosis, pyknosis, and atrophy of pyramidal cells. The histological findings corroborated with the amendment of biochemical parameters. The positive effects of LDR could offer a possible contributor in management of convulsions due to modulation of AkT/m-TOR signaling pathway, reduction of oxidative stress and modulation of brain amino acids. LDR improved the oxidative stress side effects of topiramate.

Lotfy, H. M., M. A. Hegazy, M. R. Rezk, and Y. R. Omran, "Novel spectrophotometric methods for simultaneous determination of timolol and dorzolamide in their binary mixture", Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy , vol. 126, issue 21, pp. 197-207, 2014.
Lotfy, H. M., H. H. Monir, A. M. Michael, Y. M. Fayez, and C. K. Nessim, "Impact Study of Mathematical Manipulation on the Resolution Efficiency of the Spectrophotometric Technique—An Application on Veterinary Binary Mixture with Overlapping Absorption Bands", Journal of AOAC International, vol. 103, issue 5, pp. 1412-1423, 2020.
Lotfy, S., B. Omar, S. Mohamed, and A. Saad, "I-Lived experiences of Egyptian \women with breast cancer receiving chemotherapy", Indian Journal of Publir Health Research & Development lnstitute of Medico-Legal Publications, vol. 10, issue 6, pp. 678-682, 2019.
Lotfy, H. M., M. A. Hegazy, S. Mowaka, and E. H. Mohamed, "Novel spectrophotometric methods for simultaneous determination of amlodipine, valsartan and hydrochlorothiazide in their ternary mixture", Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, vol. 140: Elsevier, pp. 495-508, 2015. Abstract
n/a
Tourism