Publications

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Hussein, H. E., F. K. Adham, G. A. Scoles, M. W. Ueti, C. E. Suarez, F. D. Guerrero, and R. G. Bastos, "Targeted silencing of the Aquaporin 2 gene of Rhipicephalus (Boophilus) microplus reduces tick fitness", Parasites & vectors, vol. 8, issue doi:10.1186/s13071-015-1226-2, pp. 618-630, 2015. aquaporin2_full_text.pdf
Hussein, B., D. Negrut, and A. A. Shabana, "Implicit and explicit integration in the solution of the absolute nodal coordinate differential/algebraic equations", Nonlinear Dynamics, vol. 54, no. 4: Springer, pp. 283–296, 2008. Abstract
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Hussein, O. G., Y. Rostom, M. A. Kawy, M. R. Rezk, and D. A. Ahmed, "Spectrophotometric platform windows' exploitation for the green determination of Alcaftadine in presence of its oxidative degradation product.", Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, vol. 297, pp. 122737, 2023. Abstract

This study presents the determination of Alcaftadine (ALF) in its oxidative degradation product presence by applying comprehensive study comparative of four different green stability indicating spectrophotometric approaches through successful exploitation of different spectrophotometric platform windows. Window I; based on absorption spectrum zero order data manipulation using the newly developed extended absorbance difference (EAD). Window II; based on derivative spectra by second order derivative (D) data manipulation. Window III; based on ratio spectra applying constant multiplication (CM) and absorptivity centering via factorized ratio difference spectrum (ACT-FSR) methods data manipulation. Finally, window IV; based on derivative of ratio spectrum by virtue of first derivative of ratio spectral (DD) method data manipulation. Calibration curves construction were over linearity range; 1.0-14.0 µg/mL for ALF. The proposed methods accuracy, precision, and linearity range were determined and validated as per ICH guidelines. Moreover, they were able to analyze ALF in raw form, dosage form and in existence of its oxidative degradation product. Statistical comparisons were done between the proposed methods and the reported one showing no significant difference concerning accuracy and precision. Furthermore, greenness profile assessment was accomplished by means of four metric tools; namely: analytical greenness (AGREE), green analytical procedure index (GAPI), analytical eco-scale, and national environmental method index (NEMI).

Hussein, A. H., A. El-Sanousi, A. A. Youssif, M. A. Shalaby, M. S. Saber, and I. M. Reda, "Sequence Analysis of Fusion and Matrix Protein Genes of the Velogenic Viscerotropic Newcastle Disease Virus Egyptian Strain sr/76", In the First International Conference of Virology "Towards A Better Strategy for Control of Viral Diseases", Cairo, Egypt, pp. 65, 2004.
Hussein, R. Z. K., N. K. Attia, M. K. Fouad, and S. T. Elsheltawy, "Experimental investigation and process simulation of biolubricant production from waste cooking oil", Biomass and Bioenergy, vol. 144: Pergamon, pp. 105850, 2021. Abstract
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Hussein, O. G., D. A. Ahmed, M. R. Rezk, M. A. Kawy, and Y. Rostom, "Exquisite integration of quality-by-design and green analytical approaches for simultaneous determination of xylometazoline and antazoline in eye drops and rabbit aqueous humor, application to stability study.", Journal of pharmaceutical and biomedical analysis, vol. 235, pp. 115598, 2023. Abstract

This work implements a stability indicating HPLC method developed to simultaneously determine xylometazoline (XYLO) and antazoline (ANT) in their binary mixture, rabbit aqueous humor and cited drug's degradates by applying analytical quality-by-design (AQbD) combined with green analytical chemistry (GAC) experiment for the first time. This integration was designed to maximize efficiency and minimize environmental impacts, as well as energy and solvent consumption. Analytical quality-by-design was applied to achieve our aim starting with evaluation of quality risk and scouting analysis, tracked via five parameters chromatographic screening using Placket-Burman design namely: pH, temperature, organic solvent percentage, flow rate, and wavelength detection. Recognizing the critical method parameters was done followed by optimization employing central composite design and Derringer's desirability toward assess optimum conditions that attained best resolution with satisfactory peak symmetry with short run time. Optimal chromatographic separation was attained by means of an XBridge® C18 (4.6 × 250 mm, 5 µm) column through isocratic elution using a mobile phase consists of phosphate buffer (pH 3.0): ethanol (60:40, by volume) at a 1.6 mL/min flow rate and 230.0 nm UV detection. Linearity acquired over a concentration range of 1.0-100.0 µg/mL and 0.5-100.0 µg/mL for XYLO and ANT, respectively. Furthermore, imperiling cited drugs' stock solutions to stress various conditions and satisfactory peaks of degradation products were obtained indicating that cited drugs are vulnerable to oxidative degradation and basic hydrolysis. Degradates' structures were elucidated using mass spectrometry. Applying various assessment tools; namely: analytical greenness (AGREE), green analytical procedure index (GAPI), analytical eco-scale, and national environmental method index (NEMI), Greenness method's evaluation was applied and proved to be green. In fact, the developed method is established to be perceptive, accurate, and selective to assess cited drugs for routine analysis.

Hussein, G. M., S. S. Muhammad, N. A. Gomaa, M. R. Shehata, and W. M. Hosny, "A Study on the extraction of uranium(VI) from sulphate leach liquor using LIX63", JOURNAL OF DISPERSION SCIENCE AND TECHNOLOGY, vol. 38, issue 6, pp. 866–875, 2017. a_study_on_the_extraction_of_uranium_vi_from_sulphate_leach_liquor_using_lix63_2.pdf
Hussein, M. F., "Nile Water Management Assisted by Isotope Hydrogeochemical Data", Hydro Eco'2015, Vienna, Austria, 17 April, 2015. vienna_poster_april_2015_3.ppt
Hussein, N. T., and A. A. El-Midany, "Significance of conditioning pretreatment on enrichment and flotation of oil shale", PETROLEUM SCIENCE AND TECHNOLOGY, vol. 38, issue 10, pp. 713-722, 2020.
Hussein, E., and J. Teruya, "Weak D types in the Egyptian population.", American journal of clinical pathology, vol. 139, issue 6, pp. 806-11, 2013 Jun. Abstract

Patients with the most common weak D types 1, 2, and 3 can be safely considered D positive. We evaluated 1,113 Rh-negative Egyptian samples for weak D expression to propose a cost-effective strategy related to D variant testing. D variants were tested using polymerase chain reaction with sequence-specific priming. Fifty samples were D variants (4.5%): weak D type 4.2 (32%), weak D type 4.0/4.1 (16%), and weak D type 15 (2%). Fifteen (62.5%) of 24 samples were identified serologically as partial D. We also studied the probability of the development of anti-D in 52 Rh-negative children with thalassemia who were receiving units for which weak D was not tested. Anti-D alloimmunization was observed in 63.5% of patients with thalassemia. It is prudent to implement weak D typing in Egyptian donors. Weak D variants of Egyptians are significantly different compared with Caucasians. Ethnicity must be taken into consideration when developing clinical and prenatal strategies related to D variants.

Hussein, M. M., and S. M. Metwalli, "Interactive Belt Drive Mechanics Using CAD Program and Finite Element Validation", Assiut University International Conference MEATIP 5, March 29-31, Assiut, Egypt, Assiut University, pp. 301-311, 2011.
Hussein, M. E., E. A. S. Senousy, W. H. Abd-Elslam, K. A. Ahmed, H. I. El-Askary, S. M. Mouneir, and E. A. M. Fishawy, "Roselle Seed Oil and its Nano-Formulation Alleviated Oxidative Stress, Activated Nrf2 and Downregulated m-RNA Expression Genes of Pro-inflammatory Cytokines in Paracetamol-intoxicated Rat Model", Records of Natural Products, vol. 14, issue 1, pp. 1-17, 2020. roselle_seed_oil_and_its_nano-formulation_records_of_natural_products.pdf
Hussein, M. H. E., A. K. Hegazy, and H. F. Kabiel, Radionuclides uptake by some crops raised in coastal black sand soil, , Deutschland/Germany, LAMBERT Academic Publishing, 2016. radionuclides_uptake_by_crops.pdf
Hussein, S., A. Prince, A. ElBehairy, N. S. Kotb, and M. Bahaa, "Lipopolysaccharide-induced Alterations of Immune-related Genes in Goat. ", D. J. of Biomed. Res. , vol. 4, issue 1, pp. 1-5, 2017. 6._lipopolysaccharide-induced_alterations_of_immune-related_genes_in_goat.pdf
Hussein, M. F., N. A. Ahmed, and S. M. Rawi, "Effect of the organophosphorus insecticide "cyolane" on the ‎inhibitory glycine and alanine in rat brain. ‎", Proc. Zool. Soc. A.R.E. , vol. 8, pp. 140-183, 1985.
Hussein, E. R. M. A., M. A. Raafat, I. M. Elgindi, and M. S. M. Samy, "Metabolism of P23 in sheep", Pakistan J Sci Res, 1968. Abstract
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Hussein, M. E., O. G. Mohamed, A. M. El-Fishawy, H. I. El-Askary, A. S. El-Senousy, A. A. El-Beih, E. S. Nossier, A. M. Naglah, A. A. Almehizia, and A. Tripathi, "Identification of Antibacterial Metabolites from Endophytic Fungus Aspergillus fumigatus, Isolated from Albizia lucidior Leaves (Fabaceae), Utilizing Metabolomic and Molecular Docking Techniques", Molecules, vol. 27, issue 3: MDPI, pp. 1117, 2022. Abstract
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Hussein, G. M. H., M. A. Sayed, T. M. A. Abdel-Rahman, and A. H. Alwan, "Induction of acquired resistance in strawberry calli by elicitation of fungal homogenates", Journal of Food, Agriculture & Environment, vol. 10, issue (3&4), pp. 132-137, 2012.
Hussein, H. A., E. Frost, B. Talbot, M. Shalaby, E. Cornaglia, and Y. EL-Azhary, "Comparison of polymerase chain reaction and monoclonal antibodies for G-typing of group A bovine rotavirus directly from fecal material.", Veterinary microbiology, vol. 51, issue 1-2, pp. 11-7, 1996 Jul. Abstract

A reverse transcriptase and polymerase chain reaction (RT-PCR)-based assay for G-typing of bovine rotaviruses (BRV) was compared with a monoclonal antibody-based immunoassay (MAbs-ELISA) in the characterization of BRV field strains obtained from calves in different regions of Quebec between 1992 and 1994. The strains were analysed for two G types (G6 and G10) which are the most predominate BRV field strains. Fecal samples positive for BRV by polyacrylamide gel electrophoresis (n = 74) were typed by both methods revealing 77% correlation. RT-PCR detected 10 more G6 and 2 more G10 serotypes than MAbs-ELISA. Nine of the 12 discrepant samples could be cultivated and were confirmed as G6 (8) or G10 (1) by both methods. RT-PCR was able to efficiently detect artificial mixes of G6 and G10 and detected two mixed field infections. Four additional infections considered as mixed by MAbs-ELISA and as only G6 by RT-PCR were possibly MAbs-ELISA cross-reactions. RT-PCR provided a very sensitive method for typing BRV field isolates.

Hussein, H. A., M. M. Youssef, A. H. Osman, E. A. El-Ebiary, and M. A. Shalaby, "Immunopathogenesis of attenuated strain of chicken infectious anemia virus in one day old specific pathogen free chicks", Egypt. J. immunol., vol. 10, pp. 89-102 , 2003.