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Hussein, A., V. Gaudet, H. Mostafa, and M. Elmasry, "A 16-bit High Speed Low Power Hybrid Adder", IEEE International Conference on Microelectronics (ICM 2016), Cairo, Egypt, IEEE, pp. 313-316, 2016. [PDF]
Hussein, G. M., S. S. Muhammad, N. A. Gomaa, M. R. Shehata, and W. M. Hosny, "A study on the extraction of uranium (VI) from sulphate leach liquor using LIX63", Journal of Dispersion Science and Technology, vol. 38, issue 6: Taylor & Francis, pp. 866-875, 2017. Abstract
Hussein, A. _el_ela A., N. R. Darwish, and H. A. Hefny, "Multiple-Published Tables Privacy-Preserving Data Mining: A Survey for Multiple-Published Tables Techniques", International Journal of Advanced Computer Science and Applications, issue 6, 2015.
Hussein, S., A. Prince, A. ElBehairy, N. S. Kotb, and M. Bahaa, "Lipopolysaccharide-induced Alterations of Immune-related Genes in Goat. ", D. J. of Biomed. Res. , vol. 4, issue 1, pp. 1-5, 2017. 6._lipopolysaccharide-induced_alterations_of_immune-related_genes_in_goat.pdf
Hussein, H. A., M. M. Emara, M.A. Rohaim, K. Ganapathy, and A. M. Arafa, "Sequence Analysis of Infectious Bronchitis Virus (IBV) IS/1494 Like Strain Isolated From Broiler Chicken Co-infected With Newcastle Disease Virus (NDV) In Egypt during 2012", International Journal of Poultry Science, 2014.
Hussein, A. A., C. J. Welty, N. Ameli, J. E. Cowan, M. Leapman, S. P. Porten, K. Shinohara, and P. R. Carroll, "Untreated Gleason Grade Progression on Serial Biopsies during Prostate Cancer Active Surveillance: Clinical Course and Pathological Outcomes.", The Journal of urology, vol. 194, issue 1, pp. 85-90, 2015 Jul. Abstract

PURPOSE: We describe the outcomes of patients with low risk localized prostate cancer who were upgraded on a surveillance biopsy while on active surveillance and evaluated whether delayed treatment was associated with adverse outcome.

MATERIALS AND METHODS: We included men in the study with lower risk disease managed initially with active surveillance and upgraded to Gleason score 3+4 or greater. Patient demographics and disease characteristics were compared. Kaplan-Meier curve was used to estimate the treatment-free probability stratified by initial upgrade (3+4 vs 4+3 or greater), Cox regression analysis was used to examine factors associated with treatment and multivariate logistic regression analysis was used to evaluate the factors associated with adverse outcome at surgery.

RESULTS: The final cohort comprised 219 men, with 150 (68%) upgraded to 3+4 and 69 (32%) to 4+3 or greater. Median time to upgrade was 23 months (IQR 11-49). A total of 163 men (74%) sought treatment, the majority (69%) with radical prostatectomy. The treatment-free survival rate at 5 years was 22% for 3+4 and 10% for 4+3 or greater upgrade. Upgrade to 4+3 or greater, higher prostate specific antigen density at diagnosis and shorter time to initial upgrade were associated with treatment. At surgical pathology 34% of cancers were downgraded while 6% were upgraded. Cancer volume at initial upgrade was associated with adverse pathological outcome at surgery (OR 3.33, 95% CI 1.19-9.29, p=0.02).

CONCLUSIONS: After Gleason score upgrade most patients elected treatment with radical prostatectomy. Among men who deferred definitive intervention, few experienced additional upgrading. At radical prostatectomy only 6% of cases were upgraded further and only tumor volume at initial upgrade was significantly associated with adverse pathological outcome.

Hussein, M. M. M., and M. S. AzzaArafa, "Nitrogenous nutrition of paspalum turfgrass grown in sandy soil using chemical and biofertilizers", Annals of Agrie. Sci., Moshtohor, vol. 45, pp. 337-351, 2007. Abstract
Hussein, A., M. Fawzy, W. M. Ismail, M. Refky, and H. Mostafa, "A 4-Bit 6GS/s Time-Based Analog-To-Digital Converter", IEEE International Conference on Microelectronics (ICM 2014), Doha, Qatar, IEEE, pp. 92-95, 2014. [PDF]
Hussein, H. E., G. A. R. Bohaliga, W. C. Johnson, and R. O’Connor, "Identification of a putative methyltransferase gene of Babesia bigemina as a novel molecular biomarker uniquely expressed in parasite tick stages", Parasites & Vectors , vol. 11, pp. 480-493, 2018. identification.pdf
Hussein, A. A., S. Dibaj, N. Hinata, E. Field, K. O'leary, B. Kuvshinoff, J. L. Mohler, G. Wilding, and K. A. Guru, "Development and Validation of a Quality Assurance Score for Robot-assisted Radical Cystectomy: A 10-year Analysis.", Urology, vol. 97, pp. 124-129, 2016 Nov. Abstract

OBJECTIVE: To develop quality assessment tool to evaluate surgical performance for robot-assisted radical cystectomy program.

METHODS: A prospectively maintained quality assurance database of 425 consecutive robot-assisted radical cystectomies performed by a single surgeon between 2005 and 2015 was retrospectively reviewed. Potentially modifiable factors, related to the management and perioperative care of patients, were used to evaluate patient care. Criteria included the following: preoperative (administration of neoadjuvant chemotherapy); operative (operative time <6.5 hours and estimated blood loss <500 cc); pathologic (negative soft tissue surgical margins and lymph node yield ≥20); and postoperative (no high-grade complications, readmission, or noncancer-related mortality within 30 days).The Quality Cystectomy Score (QCS) was developed (1 star: achieving ≤2 criteria or mortality within 30 days; 2 stars: 3 or 4 criteria met; 3 stars: 5 or 6 criteria met; and 4 stars: 7 or all criteria met). Univariate and multivariate Cox proportional hazard regression models were fitted to test for the association between QCS and survival outcomes.

RESULTS: Most patients (85%) achieved at least 3 stars, and more patients achieved 4 stars with time. High QCS was associated with better recurrence-free, cancer-specific, and overall survival (P values <.05). None of the patients with 1-star were alive at 1 year. Patients with 4 stars achieved the best survival rates (recurrence-free survival [62%], cancer-specific survival [70%], and overall survival [53%] at 5 years) (log rank P < .0001).

CONCLUSION: Continuous assessment for quality improvement facilitated implementation and maintenance of robot-assisted program for bladder cancer.

Hussein, A. A. E. - D. M., "Egyptian Education in The Sudan from 1943 to 1952 ", Egyptian Society for Historical Studies , vol. Annual Symposium of the Egyptian Society for Historical Studies for 2006, issue Annual Symposium 2006, pp. 30-60, 2006. ltlym_lmsr_f_lswdn.pdf
Hussein, O., N. Hamza, and H. Hefny, "Limitations of Current Security Measures to Address Information Leakage Attacks", International Journal of Computer Science and Information Security, vol. 12, issue 8, 2014.
Hussein, Y., E. M. El-Fakharany, E. A. Kamoun, S. A. Loutfy, R. Amin, T. H. Taha, S. A. Salim, and M. Amer, "Electrospun PVA/hyaluronic acid/L-arginine nanofibers for wound healing applications: Nanofibers optimization and in vitro bioevaluation.", International journal of biological macromolecules, vol. 164, pp. 667-676, 2020. Abstract

Hyaluronic acid (HA) based nanofibers (NFs) represented a novel class of bioactive wound dressings that have a vital role in wound management due to their unique properties as an extracellular-matrix and accelerating wound healing. Novel L-arginine-loaded citric acid crosslinked PVA-HA NFs were fabricated by electrospinning and proposed for potential wound healing purposes. However, poor mechanical properties of HA NFs might limit its biological usage, thus this study aims to develop reinforced PVA/HA NFs by incorporation of cellulose nanocrystals (CNCs) as nanofiller and loading L-arginine as wound healing accelerator. Results revealed that incorporation of CNCs into PVA/HA significantly improved mechanical and swelling properties of NFs, compared to CNC-free NFs. Biological performance of NFs was evaluated on normal human skin melanocyte (HFB-4) and lung fibroblast (WI38) cell-lines. PVA/HA/CNC/L-arginine NFs exhibited excellent hemocompatibility, high protein adsorption, outstanding proliferative and adhesive potential on HFB-4 cells expressed by high wound gap-closure 99.9% after 48 h of exposure. Released arginine from PVA/HA/CNC NFs showed sustained release about 46.5% and ~90% after 24 h and 48 h, respectively. Briefly, PVA/HA/CNCs/L-arginine showed adequate antibacterial activity especially against Klebsiella pneumonia, as an acute popular pathogen causing a skin infection. These results indicate that PVA/HA/CNC/L-arginine could act as promising and multifunctional wound dressings.

Hussein, A. F. A., N. N. Nawar, M. M. A.Haleim, and R. H. Elsherif, Evaluation of a Chromogenic Culture Medium Versus Polymerase Chain Reaction for Diagnosis of Clostridium Difficile in Antibiotic Induced Diarrhea, , Giza, Cairo univ , 2012. Abstract3604-7054-1-sm.pdf

Key words: Antibiotic associated diarrhea-Clostridium Difficile-
Polymerase chain reaction-Chromogenic culture media.
Background: Antibiotic associated diarrhea (AAD) can be a significant
problem resulting in incomplete duration of therapy and development of
microbial resistance and can cause severe complications e.g. electrolyte
imbalances, dehydration, pseudomembranous colitis, toxic megacolon or
even death.
Clostridium Difficile is the leading cause of antibiotic associated diarrhea
in hospitalized patients.
Materials and Methods: In this work we aimed to evaluate
Chromogenic agar versus polymerase chain reaction in diagnosis of
Clostridium Difficile infection.
The study included 100 cases of antibiotic associated diarrhea and 20
completely healthy individuals as control group
Results: The results was that by PCR for cases, 2/100 cases were positive
for toxin B,and one/100 case positive for Binary toxin, no cases were
positive for toxin A, 2/100 cases were positive for tpi gene, for control
group no samples were positive for any toxin or tpi gene.

Hussein, M. F., N. A. Ahmed, and S. M. ‎ Rawi, "The alterations of rat brain GABA and glutamine induced by ‎the organophosphorus compound cyolane.‎", Comp. Biochem. Physiol. , vol. 84C, issue 1, pp. 165-170‎, 1986.
Hussein, M. M., R. M. Gaafar, A. M. Abdel-Warith, W. A. Ahmed, Nasr Mohamed Ali Allahloubi, Ahmed Mohamed Abdel Warith, S. E. Salem, and I. M. Abdel-Salam, "Efficacy and Toxicity of Metronomic Chemotherapy in Metastatic Breast Cancer: Egyptian Experience.", Clinical breast cancer, vol. 17, issue 8, pp. 618-628, 2017 Dec. Abstract

BACKGROUND: Metronomic chemotherapy (MC) has shown efficacy in patients with metastatic breast cancer (MBC). We therefore tested the efficacy and toxicity of MC in pretreated MBC.

PATIENTS AND METHODS: This prospective phase II study included 50 patients with heavily pretreated MBC who received MC in the form of continuous oral cyclophosphamide 50 mg/day and oral methotrexate 2.5 mg twice per day on days 1 and 2 every week. The primary end point was progression-free survival (PFS), whereas the secondary end points were response rate, overall survival (OS), and safety.

RESULTS: Forty-eight patients were assessed. One patient achieved complete response and 10 patients had partial response, whereas 19 patients had stable disease. The median PFS was 5 months, whereas the median OS was 7 months. Patients with negative progesterone receptors, Eastern Cooperative Oncology Group performance status (PS) 1, achieving response, and those who developed leucopenia, neutropenia, and anemia had significant prolonged PFS, whereas patients with early stage at presentation, receiving < 5 previous treatment lines, achieving response, and experiencing anemia with MC had significant superior OS. In multivariate analysis, achieving response, PS 1, a longer time interval from initial diagnosis until starting MC, and anemia were independent prognostic factors for longer PFS. Initial stage at presentation, number of previous treatment lines, and response were independent prognostic factors for OS.

CONCLUSIONS: MC is an attractive treatment approach that is effective and less toxic. There are certain groups of patients who seem to benefit more, especially those who experienced toxicity with treatment. Larger trials are warranted to assess this approach early in the course of the disease and with other more active agents.

Hussein, M. A., I. M. Eissa, and A. A. Dahab, "Vision-Threatening Behcet's Disease: Severity of Ocular Involvement Predictors.", Journal of ophthalmology, vol. 2018, issue 2018, pp. 9518065 / 6 pages, 2018. Abstract

Purpose: To examine and spot systemic findings commonly associated with a serious form of ocular Behcet's disease. This could potentially help ophthalmologists categorize their patients based on future risk and plan treatment accordingly.

Subjects and Methods: The data of 249 patients with Behcet's disease were examined thoroughly. Correlations between systemic and ocular findings were recorded. Patients were further subgrouped by the authors as having a vision-threatening form of the disease or not. Regression analysis was done to spot predictors for a vision-threatening form of the disease.

Results: The presence of systemic vasculitis and oral and genital ulcers in a patient with Behcet's disease was found to be associated with a milder form of ocular affection or none at all and vice versa. Certain correlations between findings were also found.

Conclusion: Certain findings in Behcet's disease may act as predictors for the severity of ocular affection. Directing our attention to these factors by the internist and ophthalmologist can help plan the frequency of follow-up as well as the aggressiveness of treatment in patients with Behcet's disease.

Hussein, A. A., N. Hinata, S. Dibaj, P. R. May, J. D. Kozlowski, H. Abol-Enein, R. Abaza, D. Eun, M. S. Khan, J. L. Mohler, et al., "Development, validation and clinical application of Pelvic Lymphadenectomy Assessment and Completion Evaluation: intraoperative assessment of lymph node dissection after robot-assisted radical cystectomy for bladder cancer.", BJU international, vol. 119, issue 6, pp. 879-884, 2017 Jun. Abstract

OBJECTIVES: To develop a scoring tool, Pelvic Lymphadenectomy Appropriateness and Completion Evaluation (PLACE), to assess the intraoperative completeness and appropriateness of pelvic lymph node dissection (PLND) following robot-assisted radical cystectomy (RARC).

PATIENTS, SUBJECTS AND METHODS: A panel of 11 open and robotic surgeons developed the content and structure of PLACE. The PLND template was divided into three zones. In all, 21 de-identified videos of bilateral robot-assisted PLNDs were assessed by the 11 experts using PLACE to determine inter-rater reliability. Lymph node (LN) clearance was defined as the proportion of cleared LNs from all PLACE zones. We investigated the correlation between LN clearance and LN count. Then, we compared the LN count of 18 prospective PLNDs using PLACE with our retrospective series performed using the extended template (No PLACE).

RESULTS: A significant reliability was achieved for all PLACE zones among the 11 raters for the 21 bilateral PLND videos. The median (interquartile range) for LN clearance was 468 (431-545). There was a significant positive correlation between LN clearance and LN count (R2 = 0.70, P < 0.01). The PLACE group yielded similar LN counts when compared to the No PLACE group.

CONCLUSIONS: Pelvic Lymphadenectomy Appropriateness and Completion Evaluation is a structured intraoperative scoring system that can be used intraoperatively to measure and quantify PLND for quality control and to facilitate training during RARC.

Hussein, R. S., and A. Mahrous, "Users’ Engagement on Facebook: A Cluster Analysis", International Journal of Business and Emerging Markets, vol. 8, issue 4, pp. 426-445, 2016.
Hussein, G. M., G. A. Abu El-Heba, S. M. Abdou, and N. A. Abdallah, "Optimization of transient gene expression system in Gerbera jemosonii petals", GM crops & food, vol. 4, issue 1: Taylor & Francis, pp. 50-57, 2013. Abstract
Hussein, H. A., H. A. Sultan, A. H. El-deeb, A. A. El-Sanousi, and others, "Possible causes of re-emerging outbreaks of H5N1 avian influenza virus in vaccinated chickens in Sharkia Governorate in Egypt.", International Journal of Virology, vol. 5, no. 1: Academic Journals, pp. 36–43, 2009. Abstract