We investigated the potential association between endothelial nitric oxide synthase (eNOS) gene polymorphism and erectile dysfunction (ED). The study was carried on 60 males with ED. All patients were subjected to medical and sexual history taking including the international index of erectile function (IIEF-5) questionnaire, general and genital examination and combined injection and stimulation test (ICI). Patients with a negative response were evaluated through a penile dynamic duplex to clarify the etiology of ED. eNOS genotype polymorphism was determined by restriction fragment length polymorphism (RFLP). The mean age of patientswas 49.7 years and mean ED

Objective Coronary heart disease (CHD) is a complex disease with both environmental and genetic determinants. The aim of this study was to assess the presence of fibrinogen b-chain (FGB)-455 G/A and prothrombin (PT) 20210 G/A polymorphisms in CHD Egyptian patients and to correlate the co-existence of both polymorphisms with the clinical and laboratory data of the patients.

This work presents an investigation of the experimental measurements that carried out to study the
breakdown threshold dependence on gas pressure in the breakdown of molecular oxygen by a Nd: YAG laser source
operating at wavelengths 1064 nm with a pulse duration of 5.5 ns over a gas pressure range varies between 190-
3000 torr. The study is devoted to find out the role played by the physical processes in determining the threshold
intensity of molecular oxygen breakdown as a function of gas pressure. In doing so a previously developed electron
cascade model is modified and applied. The modification assigned the inclusion of an electron diffusion term to
account for electron losses out of the focal volume on the experimentally tested low pressure regime. Besides, the
model takes into account most of the physical processes which might take place during the interaction between the
laser beam and the oxygen molecules. The result of computations showed reasonable agreement between the
calculated threshold intensities and the experimentally measured ones over the tested gas pressure range. This
proves the validity of the model. In addition the study of the performed of the electron energy distribution function,
EEDF, and its correlated parameters viz, temporal evolution of: electron density, excitation rate, ionization rate and
electron mean energy as well as the variation of the EEDF during the laser pulse verified the exact correlation
between gas pressure and the physical process responsible for the production and loss of electrons or their energy
during the breakdown phase.

The effect of long-term treatment of chloroquine (CAS 54-05-7) (20 mg/kg body weight) on serum lipid components and its relation to to the retinotoxic effect was studied in albino rats. Chloroquine was found to form lamellar lysosome-like structures within the photoreceptive layer, as well as the pigment epithelium and neurooretinal layers. Biochemically, hypolipidemia in the serum was observed mainly due to the decrease in phospholipid portion. It was hypothesized that due to the inhibition of the degradation process in the defective lysosomes, the retinal cells were denied the re-use of their own phospholipids, and thereby resort to their uptake from the serum.

Pichia pinus biomass obtained from growth on methanol medium was fed to rats as 10% protein of the diet. Its nutritional, Physiological and histological effects were estimated. The protein efficiency ratio (PER) and the apparent digestibility of the biomass (Dapp) were 2.74 ± 0.107 and 73.99 ± 1.21, respectively, while that of casein (control) were 2.91 ± 0.127 and 90.16 ± 0.64, respectively. the alkaline phosphatase levels mostly showed no significant difference from the control. While the GOT levels were mostly significantly lowered, and the GPT levels did not show consistency. The urea nitrogen and creatinine levels in serum were unchanged, While that of uric acid was only significantly increased after 35 feeding days. Histologically no changes were observed.

Adult peripheral blood contains a limited number of endothelial progenitor cells that can be isolated for treatment of ischemic diseases. The adipose tissue became an interesting source of stem cells for regenerative medicine. This study aimed to investigate the phenotype of cells obtained by culturing adipose-derived mesenchymal stem cells (ad-MSCs) in the presence of endothelial growth supplements compared to endothelial cells obtained from umbilical cord blood (UCB). Passage 3 ad-MSCs and mononuclear layer from UCB were cultured in presence of endothelial growth media for 3 weeks followed by their characterization by flow cytometry and polymerase chain reaction. After culture in endothelial inductive media, ad-MSCs expressed endothelial genes and some endothelial marker proteins as CD31 and CD34, respectively. Adipose tissue could be a reliable source for easy obtaining, expanding and differentiating MSCs into endothelial-like cells for autologous cell-based therapy.

Serum ferritin, Alpha-1 Acid Glycoprotein (α1AGP), c-reactive protein (CRP), and ceruloplasmin (CER) were studied in 83 children with an age range of three to thirty six months. Fifty nine cases suffered from protein energy Malnutrition (PEM), 29 marasmus and 30 kwashiorkor (KWO). Twenty four healthy children were included as controls. the malnourished group exhibited significant reduction in anthropometric measurement scores and in serum albumin and prealbumin levels than those of the control group. CER was significantly reduced in PEM cases. Evidence of infection by an elevated α1AGP, A positive CRP and the preence of diarrhea was also demonstrated in the malnourished group but not in the control one. Ferritin level was significantly elevated in the KWO group, While in the marasmus one the elevation was non-significant. At the same time 100% of the KWO and 89.7% of the marasmus cases had a hemoglobin level of less than 11 g/dL. Also 46.4% of the KWO and 48.2% of the marasmus cases had a serum iron of less than 50 µg/dL. In such circumstances the elevation in serum ferritin level, in accordance with other acute phase proteins, could reflect increased secretion and/or possible leakage from damaged tissues especially when evidence of increased body iron stores is lacking.

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